Characterization of the Liver Tissue Interstitial Fluid (TIF) Proteome Indicates Potential for Application in Liver Disease Biomarker Discovery

2010 ◽  
Vol 9 (2) ◽  
pp. 1020-1031 ◽  
Author(s):  
Wei Sun ◽  
Jie Ma ◽  
Songfeng Wu ◽  
Dong Yang ◽  
Yujuan Yan ◽  
...  
Keyword(s):  
Liver Disease ◽  
Liver Tissue ◽  
Interstitial Fluid ◽  
Biomarker Discovery ◽  
Disease Biomarker ◽  
Tissue Interstitial Fluid

scholarly journals Characterization of LC-MS based urine metabolomics in healthy subjects across the life span

2020 ◽  
Author(s):  
Xiaoyan Liu ◽  
Xiaoyi Tian ◽  
Qinghong Shi ◽  
Haidan Sun ◽  
Jing Li ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
High Resolution Mass Spectrometry ◽  
Estrogen Metabolism ◽  
Healthy Children ◽  
Urine Metabolites ◽  
Clinical Cohort ◽  
Disease Biomarker ◽  
Gender And Age ◽  
Urine Metabolomics

AbstractPrevious studies reported that gender and age could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. In this study, we provided a comprehensive lifespan characterization of urine metabolomics in a cohort of 348 healthy children and 315 adults aged 1 to 70 years using liquid chromatography coupled with high resolution mass spectrometry. Our results suggested that gender-dependent urine metabolites are much greater in adults than in children. The pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the old stage. Based on the above analysis, metabolomic characteristics of each age stage were provided. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.

scholarly journals Characterization of LC-MS Based Urine Metabolomics in Healthy Subjects Across the Life Span

2020 ◽  
Author(s):  
Xiaoyan Liu ◽  
Xiaoyi Tian ◽  
Qinghong Shi ◽  
Haidan Sun ◽  
Jing Li ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
High Resolution Mass Spectrometry ◽  
Estrogen Metabolism ◽  
Healthy Children ◽  
Urine Metabolites ◽  
Clinical Cohort ◽  
Disease Biomarker ◽  
Gender And Age ◽  
Urine Metabolomics

Abstract Background: Previous studies reported that gender and age could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. Results: In this study, we provided a comprehensive lifespan characterization of urine metabolomics in a cohort of 348 healthy children and 315 adults aged 1 to 70 years using liquid chromatography coupled with high resolution mass spectrometry. Our results suggested that gender-dependent urine metabolites are much greater in adults than in children. The pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the old stage. Conclusion: Based on the above analysis, metabolomic characteristics of each age stage were provided. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.

scholarly journals In-depth proteomic analysis of tissue interstitial fluid for hepatocellular carcinoma serum biomarker discovery

2017 ◽  
Vol 117 (11) ◽  
pp. 1676-1684 ◽  
Author(s):  
Jian Zhang ◽  
Ning Hao ◽  
Wei Liu ◽  
Min Lu ◽  
Longqin Sun ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Proteomic Analysis ◽  
Interstitial Fluid ◽  
Biomarker Discovery ◽  
Serum Biomarker ◽  
Tissue Interstitial Fluid

scholarly journals Characterization of LC-MS Based Urine Metabolomics in Healthy Children and Adults

2021 ◽  
Author(s):  
Xiaoyan Liu ◽  
Xiaoyi Tian ◽  
Qinghong Shi ◽  
Haidan Sun ◽  
Jing Li ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
Estrogen Metabolism ◽  
Healthy Children ◽  
Age Group ◽  
Urine Metabolites ◽  
Disease Biomarker ◽  
Gender And Age ◽  
Comprehensive Characterization ◽  
Urine Metabolomics

Abstract Previous studies reported that gender, age, diets or lifestyles could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. In this study, we provided a comprehensive characterization of urine metabolomics in a cohort of 348 healthy children (Aged 1~18) and 315 adults (Aged 20-78) for evaluation gender and age effects. Our results suggested that urine metabolites showed larger gender differences in children than in adults. For both male/boy and female/girl, each age group showed specific metabolic characterization. Especially, the pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the old stage. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.

Detection and characterization of Leptospira spp. in dogs diagnosed with kidney and/or liver disease in Selangor, Malaysia

2021 ◽  
pp. 104063872110245
Author(s):  
Sabri A. Rahman ◽  
Kuan H. Khor ◽  
Siti Khairani-Bejo ◽  
Seng F. Lau ◽  
Mazlina Mazlan ◽  
...  
Keyword(s):  
Liver Disease ◽  
Mortality Rate ◽  
Direct Detection ◽  
Bacterial Disease ◽  
Pathogenic Leptospira ◽  
Wide Range ◽  
Leptospira Spp ◽  
Rrna Sequencing ◽  
The Common

Leptospirosis is a serious bacterial disease that affects both humans and animals. A wide range of symptoms have been described in humans; the disease in dogs is commonly associated with kidney and/or liver disease. In Malaysia, information about the common serovars infecting dogs is limited. Therefore, we investigated the occurrences of leptospirosis in 124 pet dogs diagnosed with kidney and/or liver disease. Blood, urine, abdominal effusion, and/or kidney and liver were collected from the dogs. Based on microscopic agglutination testing, 53 of 124 (42.7%) dogs were seropositive for leptospiral exposure. Sera were frequently positive to serovars Bataviae ( n = 12), Javanica ( n = 10), and Icterohaemorrhagiae ( n = 10). Direct detection using PCR showed that 42 of 124 (33.9%) of the whole blood and 36 of 113 (31.9%) urine samples were positive for pathogenic Leptospira spp. By PCR, 2 of 23 (9.1%) kidney and 2 of 23 (9.1%) liver were positive for pathogenic Leptospira spp. Abdominal effusion from 4 dogs were PCR-positive for pathogenic Leptospira spp. The species detected were L. interrogans, L. borgpetersenii, L. kirschneri, and L. kmetyi by partial 16S rRNA sequencing. We further identified and characterized 11 Leptospira spp. isolates from 8 dogs as serovars Bataviae, Javanica, and Australis. The mortality rate of the Leptospira-infected dogs was high (18 of 53; 34%).

scholarly journals On the characterization of interstitial fluid flow in the skeletal muscle endomysium

2020 ◽  
Vol 102 ◽  
pp. 103504 ◽  
Author(s):  
Qiuyun Wang ◽  
Shaopeng Pei ◽  
X. Lucas Lu ◽  
Liyun Wang ◽  
Qianhong Wu
Keyword(s):  
Skeletal Muscle ◽  
Fluid Flow ◽  
Interstitial Fluid ◽  
Interstitial Fluid Flow
Sign in / Sign up

Export Citation Format

Share Document