MicroRNA-21 Expression, Serum Tumor Markers, and Immunohistochemistry in Canine Mammary Tumors

Abstract Background: Canine mammary tumors (CMTs) are one of the most common malignancies in dogs and are associated with significant mortality. Serum tumor markers and non-coding microRNAs have recently gained widespread popularity in human oncology studies. We aimed to investigate the expression of microRNA-21 (miR21), changes in serum tumor markers (CEA and CA 15-3), and immunohistochemistry in CMTs diagnosed by clinical examination, radiology, and histopathology. This study enrolled 17 female dogs: 10 with mammary tumors and seven controls without tumors. Blood samples were collected to measure miR-21, CEA, and CA 15-3, and histological samples were prepared for histological grading and immunohistochemistry. Results: CA 15-3 was elevated in all animals, whereas CEA levels showed no change compared with controls. miR-21 was upregulated 12.84-fold in animals with CMT. The most frequently recorded CMT was mixed type. Myoepithelial cells were identified by P63 immunoreactivity, but not SMA. High expression of miR-21 was observed with positive vimentin immunoreactivity, indicating a mesenchymal origin of the tumor cells. Conclusion: The present study showed that miR-21 was elevated to a greater extent than CA 15-3 (12.84-fold vs. threefold). Mixed-type tumors with positive vimentin immunoreactivity showed the highest miR-21 levels. These findings support the hypothesis that miR-21 may be a more sensitive, noninvasive indicator for CMT.

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MicroRNA-21 expression, serum tumor markers, and immunohistochemistry in canine mammary tumors

Veterinary Research Communications ◽

10.1007/s11259-021-09861-9 ◽

2021 ◽

Author(s):

Eman S. Ramadan ◽

Noha Y. Salem ◽

Ibrahim A. Emam ◽

Naglaa A. AbdElKader ◽

Haithem A. Farghali ◽

...

Keyword(s):

Tumor Markers ◽

Mammary Tumors ◽

Serum Tumor Markers ◽

Canine Mammary Tumors

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Serum Tumor Markers and Circulating Tumor Cells

Breast Cancer 2nd edition ◽

10.1007/978-0-387-34952-7_10 ◽

2008 ◽

pp. 309-322

Author(s):

Francisco J. Esteva ◽

Herbert A. Fritsche ◽

James M. Reuben ◽

Massimo Cristofanilli

Keyword(s):

Tumor Cells ◽

Circulating Tumor Cells ◽

Tumor Markers ◽

Serum Tumor Markers

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Tumor Chemoconversion Following Surgery, Chemotherapy, and Normalization of Serum Tumor Markers in a Woman with a Mixed Type Germ Cell Ovarian Tumor

Gynecologic Oncology ◽

10.1006/gyno.2001.6541 ◽

2002 ◽

Vol 84 (3) ◽

pp. 464-467 ◽

Cited By ~ 11

Author(s):

Yehuda Ben David ◽

Amir Weiss ◽

Lea Shechtman ◽

Eliezer Shalev

Keyword(s):

Germ Cell ◽

Tumor Markers ◽

Ovarian Tumor ◽

Mixed Type ◽

Serum Tumor Markers

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Elevated CA 19-9 Levels in Mature Cystic Teratoma of the Ovary

The International Journal of Biological Markers ◽

10.1177/172460080902400108 ◽

2009 ◽

Vol 24 (1) ◽

pp. 52-56 ◽

Cited By ~ 6

Author(s):

Min Sun Kyung ◽

Joong Sub Choi ◽

Seung Hwa Hong ◽

Hak Soon Kim

Keyword(s):

Tumor Markers ◽

Elevated Serum ◽

Cystic Teratoma ◽

Ovarian Torsion ◽

Mature Cystic Teratoma ◽

Ca 125 ◽

Clinical Value ◽

Blood Samples ◽

Serum Tumor Markers

The purpose of this study was to evaluate the clinical value of serum tumor markers in patients with ovarian mature cystic teratoma (MCT). We retrospectively evaluated 163 women who underwent surgery for MCT of the ovary between March 2003 and August 2007 and who provided preoperative blood samples for the measurement of CA 19-9 and CA 125. The rates of elevated serum CA 19-9 and CA 125 levels were 31.9% (52/163) and 13.5% (22/163), respectively. The rate of ovarian torsion was 12.9% (21/163). There were significant differences between the elevated CA 19-9 group and the normal CA 19-9 group in the diameters of the tumors and the rates of ovarian torsion. Elevated serum CA 19-9 levels correlated with larger tumor diameters and higher torsion rates. CA 19-9 may be a useful tool for the diagnosis of ovarian MCT. Elevated CA 19-9 levels appear to correlate with larger tumor diameters and higher rates of ovarian torsion.

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High sensitive detection of circulating tumor cell by multimarker lipid magnetic nanoparticles and clinical verifications

Journal of Nanobiotechnology ◽

10.1186/s12951-019-0548-1 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 2

Author(s):

Jingde Chen ◽

Lin Chen ◽

Shibin Du ◽

Jing Wu ◽

Ming Quan ◽

...

Keyword(s):

Tumor Cells ◽

Tumor Markers ◽

Separation Efficiency ◽

Circulating Tumor Cell ◽

The Self ◽

Blood Samples ◽

Combined Use ◽

Positive Rate ◽

Clinical Detection ◽

Antibody Derivatives

AbstractTumor cells with heterogeneity and diversity can express different markers. At present, positive separation of circulating tumor cells (CTC) taking EpCAM as the marker was used in most cases which could be one-sided, while this study successfully prepared four antibody-modified magnetic immunoliposomes (MIL) by using the self-assembled liposome with antibody derivatives. This study aims to explore the separation efficiency and clinical detection feasibility of single or combined use of MIL with multi-tumor markers on different tumors. Captured CTC were stained with CK-FITC, CD45-PE and DAPI, and fluorescence microscope was used for the observation, analysis and calculation. The result indicated that the CTC number positive rate in blood samples of four different magnetic balls on the same patient could be up to 87.5% in 32 patients with 14 different kinds tumors. While the effect of directly mixed separation by four kinds of magnetic balls was not satisfying. It suggested that the MIL of multi-tumor markers could be a powerful tool for CTC separation in application of tumor screening and prognosis.

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