scholarly journals High sensitive detection of circulating tumor cell by multimarker lipid magnetic nanoparticles and clinical verifications

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Jingde Chen ◽  
Lin Chen ◽  
Shibin Du ◽  
Jing Wu ◽  
Ming Quan ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Tumor Markers ◽  
Separation Efficiency ◽  
Circulating Tumor Cell ◽  
The Self ◽  
Blood Samples ◽  
Combined Use ◽  
Positive Rate ◽  
Clinical Detection ◽  
Antibody Derivatives

AbstractTumor cells with heterogeneity and diversity can express different markers. At present, positive separation of circulating tumor cells (CTC) taking EpCAM as the marker was used in most cases which could be one-sided, while this study successfully prepared four antibody-modified magnetic immunoliposomes (MIL) by using the self-assembled liposome with antibody derivatives. This study aims to explore the separation efficiency and clinical detection feasibility of single or combined use of MIL with multi-tumor markers on different tumors. Captured CTC were stained with CK-FITC, CD45-PE and DAPI, and fluorescence microscope was used for the observation, analysis and calculation. The result indicated that the CTC number positive rate in blood samples of four different magnetic balls on the same patient could be up to 87.5% in 32 patients with 14 different kinds tumors. While the effect of directly mixed separation by four kinds of magnetic balls was not satisfying. It suggested that the MIL of multi-tumor markers could be a powerful tool for CTC separation in application of tumor screening and prognosis.

Preoperative detection of circulating tumor cells in patients with colorectal carcinoma: Correlation with clinicopathologic variables and recurrence.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 10578-10578
Author(s):  
Hideyasu Sakihama ◽  
Nozomi Kobayashi ◽  
Tohru Funakoshi ◽  
Tatsushi Shimokuni ◽  
Shigenori Homma ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Peripheral Blood ◽  
Tumor Recurrence ◽  
Strong Predictor ◽  
Depth Of Invasion ◽  
Blood Samples ◽  
Positive Rate ◽  
Colorectal Cancer Patients

10578 Background: The role of circulating tumor cells (CTC) in the management of colorectal cancer patients has not fully established. The aims of this study are to investigate the relationship between the presence of CTC with clinicopathological variables and recurrence by magnetic activated cell sorting (MACS) system. Methods: Peripheral blood samples were collected from 80 patients. Enrichment of CTC was performed by direct immunomagnetic labeling of EpCAM positive cells in peripheral blood. Subsequently, double immunofluorescence for cytokeratin and CD45 was performed to detect CTC. Peripheral blood samples from twenty healthy volunteers were used as controls. Results: Preoperative positive rate of CTC was 35% while specificity was 100%. No CTC was found in peripheral blood from healthy volunteers. No correlation was found between the presence of CTC and location of tumors, grade of differentiation, vessel invasion, lymph node metastasis or TNM stages. On the other hand, the depth of invasion (0% in Tis, 11.1% in T1, 18.2% in T2 and 34.7% in T3+T4, P=0.05) and tumor recurrence (28.2% for initial operation and 88.9% for reoperative surgery for tumor recurrence, P<0.001) closely correlated with the presence of CTC. Preoperative positive rate of CTC among patients who have recurred postoperatively was 75%. Conclusions: Our results indicate that detections of CTCs correlate with the depth of invasion and tumor recurrence. Preoperative presence of CTCs might be a strong predictor for tumor recurrence.

scholarly journals Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells

2020 ◽  
Vol 21 (7) ◽  
pp. 2401 ◽  
Author(s):  
Nicolai T. Sand ◽  
Tobias B. Petersen ◽  
Sara R. Bang-Christensen ◽  
Theresa D. Ahrens ◽  
Caroline Løppke ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Cell ◽  
Cancer Progression ◽  
Magnetic Beads ◽  
Liquid Biopsies ◽  
Blood Samples ◽  
Non Invasive ◽  
Clinical Detection

Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.

scholarly journals MicroRNA-21 Expression, Serum Tumor Markers, and Immunohistochemistry in Canine Mammary Tumors

2021 ◽  
Author(s):  
Noha Y. Salem ◽  
Eman S. Ramadan ◽  
Ibrahim A. Emam ◽  
Naglaa A AbdElKader ◽  
Haithem A. Farghali ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Tumor Markers ◽  
Mixed Type ◽  
Mammary Tumors ◽  
Myoepithelial Cells ◽  
Histological Grading ◽  
Blood Samples ◽  
Serum Tumor Markers ◽  
Canine Mammary Tumors ◽  
Significant Mortality

Abstract Background: Canine mammary tumors (CMTs) are one of the most common malignancies in dogs and are associated with significant mortality. Serum tumor markers and non-coding microRNAs have recently gained widespread popularity in human oncology studies. We aimed to investigate the expression of microRNA-21 (miR21), changes in serum tumor markers (CEA and CA 15-3), and immunohistochemistry in CMTs diagnosed by clinical examination, radiology, and histopathology. This study enrolled 17 female dogs: 10 with mammary tumors and seven controls without tumors. Blood samples were collected to measure miR-21, CEA, and CA 15-3, and histological samples were prepared for histological grading and immunohistochemistry. Results: CA 15-3 was elevated in all animals, whereas CEA levels showed no change compared with controls. miR-21 was upregulated 12.84-fold in animals with CMT. The most frequently recorded CMT was mixed type. Myoepithelial cells were identified by P63 immunoreactivity, but not SMA. High expression of miR-21 was observed with positive vimentin immunoreactivity, indicating a mesenchymal origin of the tumor cells. Conclusion: The present study showed that miR-21 was elevated to a greater extent than CA 15-3 (12.84-fold vs. threefold). Mixed-type tumors with positive vimentin immunoreactivity showed the highest miR-21 levels. These findings support the hypothesis that miR-21 may be a more sensitive, noninvasive indicator for CMT.

scholarly journals Modulation of Early Neutrophil Granulation: The Circulating Tumor Cell-Extravesicular Connection in Pancreatic Ductal Adenocarcinoma

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2727
Author(s):  
Harrys Kishore Charles Jacob ◽  
John Lalith Charles Richard ◽  
Rossana Signorelli ◽  
Tyler Kashuv ◽  
Shweta Lavania ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Cluster Formation ◽  
Circulating Tumor Cell ◽  
Systemic Circulation ◽  
Cell Junction ◽  
Ductal Adenocarcinoma ◽  
Signaling Proteins ◽  
Companion Cells ◽  
Single Ctcs ◽  
Junction Proteins

Tumor cells dissociate from the primary site and enter into systemic circulation (circulating tumor cells, CTCs) either alone or as tumor microemboli (clusters); the latter having an increased predisposition towards forming distal metastases than single CTCs. The formation of clusters is, in part, created by contacts between cell–cell junction proteins and/or cytokine receptor pairs with other cells such as neutrophils, platelets, fibroblasts, etc. In the present study, we provide evidence for an extravesicular (EV) mode of communication between pancreatic cancer CTCs and neutrophils. Our results suggest that the EV proteome of CTCs contain signaling proteins that can modulate degranulation and granule mobilization in neutrophils and, also, contain tissue plasminogen activator and other proteins that can regulate cluster formation. By exposing naïve neutrophils to EVs isolated from CTCs, we further show how these changes are modulated in a dynamic fashion indicating evidence for a deeper EV based remodulatory effect on companion cells in clusters.

Controllable Synthesis of Cobalt Porphyrin Nanocrystals through Micelle Confinement Self-Assembly

MRS Advances ◽  
2020 ◽  
Vol 5 (42) ◽  
pp. 2147-2155
Author(s):  
Sudi Chen ◽  
Xitong Ren ◽  
Shufang Tian ◽  
Jiajie Sun ◽  
Feng Bai
Keyword(s):  
Self Assembly ◽  
Water Oxidation ◽  
Separation Efficiency ◽  
Noncovalent Interactions ◽  
Building Blocks ◽  
The Self ◽  
Hexagonal Prism ◽  
Assembly Process ◽  
Surfactant Micelles ◽  
Cobalt Porphyrin

AbstractThe self-assembly of optically active building blocks into functional nanocrystals as high-activity photocatalysts is a key in the field of photocatalysis. Cobalt porphyrin with abundant catalytic properties is extensively studied in photocatalytic water oxidation and CO2 reduction. Here, we present the fabrication of cobalt porphyrin nanocrystals through a surfactant-assisted interfacial self-assembly process using Co-tetra(4-pyridyl) porphyrin as building block. The self-assembly process relies on the combined noncovalent interactions such as π-π stacking and axial Co-N coordination between individual porphyrin molecules within surfactant micelles. Tuning different reaction conditions (temperature, the ratio of co-solvent DMF) and types of surfactant, various nanocrystals with well-defined 1D to 3D morphologies such as nanowires, nanorods and nano hexagonal prism were obtained. Due to the ordered accumulation of molecules, the nanocrystals exhibit the properties of the enhanced capability of visible light capture and can conduce to improve the transport and separation efficiency of the photogenerated carriers, which is important for photocatalysis. Further studies of photocatalytic CO2 reduction are being performed to address the relationship between the size and shape of the nanocrystals with the photocatalytic activity.

Establishment and clinical application of a highly sensitive TAT-2 time-resolved fluorescence immunoassay detection

2021 ◽  
Author(s):  
Xindong Chen ◽  
Jianfeng Hong ◽  
Han Zhao ◽  
Zhongyi Xiang ◽  
Yuan Qin ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Nasopharyngeal Cancer ◽  
Reaction Rates ◽  
Measurement Range ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Combined Use ◽  
Highly Sensitive ◽  
Positive Rate

Abstract Background: A rapid and highly sensitive assay for tumor-associated trypsinogen-2 (TAT-2) based on the time-resolved fluorescence immunoassay (TRFIA) detection technique was developed for the determination of serum TAT-2 levels in cancers. Results: The measurement range of TAT-2-TRFIA was 1.53-300 ng/mL. The within-run and between-run coefficients of variation of TAT-2-TRFIA were 4.38% and 7.82%, respectively. The recovery rate of TAT-2-TRFIA was 103.0%. The cross-reaction rates of trypsin and T-cell immunoglobulin mucin 3 were 0.02% and 0.82%, respectively. The TAT-2-positive rates in lung cancer, liver cancer, nasopharyngeal cancer, cholangiocarcinoma, brain cancer, and pancreatic cancer were 45.9%, 50.0%, 45.0%, 64.3%, 50.0%, and 41.7%, respectively, with the areas under ROC curves of 0.788, 0.734, 0.862, 0.720, 0.887, and 0.585, respectively. In patients with lung cancer, the positive rate of the single indicator CEA was 28.4%, which increased to 60.6% after combined use with TAT-2. In patients with cholangiocarcinoma, the positive rate of CA-199 was 35.7%, which increased to 71.4% after combined use with TAT-2. Conclusions: TAT-2 is expected to be used as an auxiliary diagnostic indicator for the combined use of tumor markers to improve the positive rate and accuracy of detection.

scholarly journals Tm-Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Yeqi Fu ◽  
Mingwei Wang ◽  
Xinxin Yan ◽  
Auwalu Yusuf Abdullahi ◽  
Jianxiong Hang ◽  
...  
Keyword(s):  
Ancylostoma Ceylanicum ◽  
Shift Method ◽  
Accuracy Test ◽  
Zoonotic Risk ◽  
Detectable Concentration ◽  
Positive Rate ◽  
Clinical Detection ◽  
Shift Detection ◽  
The Stability ◽  
Detection Effect

To develop a Tm-shift method for detection of dog-derived Ancylostoma ceylanicum and A. caninum, three sets of primers were designed based on three SNPs (ITS71, ITS197, and ITS296) of their internal transcribed spacer 1 (ITS1) sequences. The detection effect of the Tm-shift was assessed through the stability, sensitivity, accuracy test, and clinical detection. The results showed that these three sets of primers could distinguish accurately between A. ceylanicum and A. caninum. The coefficient of variation in their Tm values on the three SNPs was 0.09% and 0.15% (ITS71), 0.18% and 0.14% (ITS197), and 0.13% and 0.07% (ITS296), respectively. The lowest detectable concentration of standard plasmids for A. ceylanicum and A. caninum was 5.33 × 10−6 ng/μL and 5.03 × 10−6 ng/μL. The Tm-shift results of ten DNA samples from the dog-derived hookworms were consistent with their known species. In the clinical detection of 50 fecal samples from stray dogs, the positive rate of hookworm detected by Tm-shift (42%) was significantly higher than that by microscopic examination (34%), and the former can identify the Ancylostoma species. It is concluded that the Tm-shift method is rapid, specific, sensitive, and suitable for the clinical detection and zoonotic risk assessment of the dog-derived hookworm.

Sign in / Sign up

Export Citation Format

Share Document