scholarly journals The Temporal Structure and Association Networks of Endophytic Bacteria in Pea Roots and Nodules

2021 ◽  
Author(s):  
Xin Lv ◽  
Qiankun Wang ◽  
Xiaoyan Zhang ◽  
Junjie Hao ◽  
Li Li ◽  
...  
Keyword(s):  
Life Cycle ◽  
Bacterial Community ◽  
Endophytic Bacteria ◽  
Developmental Stages ◽  
Temporal Structure ◽  
Mature Stage ◽  
Rrna Gene ◽  
Plant Life ◽  
Endophytic Bacterial Community ◽  
Plant Life Cycle

Abstract Background and aims Endophytic bacteria from legume roots and nodules play important roles in regulating plant growth and health. However, little is known about how the endophytic bacteria community changes and how it interacts with each other throughout the plant life cycle. Methods We collected twenty pea cultivars to study the composition and structure of endophytic bacterial community during the whole developmental period using Illumina sequencing of 16S rRNA gene. Results Here we show that the development period significantly affected the structure of root endophytic bacterial community and peas recruit different root microbes during different developmental stages. The complexity of microbial community first increased and then decreased with the growth of pea. Rhizobium began to accumulate in pea seedling roots, and the content peaked at flowering stage and remained at high levels during the mature stage. In the flowering and mature stage, the relative abundance of Pseudomonas increased. Conclusions These findings can deepen the overall understanding of the community structure and interaction network of endophytic bacteria from pea root and nodules, and provide a detail for the establishment of root endophytic bacteria throughout the plant life cycle.

scholarly journals Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

2007 ◽  
Vol 73 (22) ◽  
pp. 7259-7267 ◽  
Author(s):  
Rodrigo Mendes ◽  
Aline A. Pizzirani-Kleiner ◽  
Welington L. Araujo ◽  
Jos M. Raaijmakers
Keyword(s):  
Bacterial Community ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Burkholderia Cepacia ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Biochemical Characterization ◽  
Burkholderia Cepacia Complex ◽  
Rrna Gene ◽  
Endophytic Bacterial Community

ABSTRACT Bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in Brazil. Endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. Many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16S rRNA gene sequence analysis revealed that the selected isolates of the endophytic bacterial community of sugarcane belong to the genera of Burkholderia, Pantoea, Pseudomonas, and Microbacterium. Bacterial isolates belonging to the genus Burkholderia were the most predominant among the endophytic bacteria. Many of the Burkholderia isolates produced the antifungal metabolite pyrrolnitrin, and all were able to grow at 37°C. Phylogenetic analyses of the 16S rRNA gene and recA gene sequences indicated that the endophytic Burkholderia isolates from sugarcane are closely related to clinical isolates of the Burkholderia cepacia complex and clustered with B. cenocepacia (gv. III) isolates from cystic fibrosis patients. These results suggest that isolates of the B. cepacia complex are an integral part of the endophytic bacterial community of sugarcane in Brazil and reinforce the hypothesis that plant-associated environments may act as a niche for putative opportunistic human pathogenic bacteria.

scholarly journals The Dynamics of Endophytic Bacterial Community Structure in Rice Roots under Different Field Management Systems

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1623
Author(s):  
Guan-Ying Lin ◽  
Chia-Yu Lin ◽  
Su-Jein Chang ◽  
Wei-Yi Lin
Keyword(s):  
Bacterial Community ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Stage ◽  
Bacterial Community Composition ◽  
Farming Systems ◽  
Rrna Gene ◽  
Rice Roots ◽  
Field Management ◽  
Endophytic Bacterial Community

The structure of endophytic bacteria is important to plant health and stress tolerance, but little is known about the impacts of farming systems on temporal changes in diversity of endophytic bacteria. In this study, we investigated the endophytic microbiome in rice roots under organic and conventional farming systems at the seedling and tillering stages over two consecutive cropping seasons using 16S rRNA gene sequencing. Although the composition of the microbiome was significantly influenced by a field management system, growth stage, and interaction between the two factors, the growth stage alone explained more than 40% of variance. Soil pH and organic matter content also partially influenced the endophytic bacterial community composition. In addition, through indicator species analysis and the functional profiles predicted by phylogenetic analysis, we revealed the selection force of rice roots at the tillering stage to recruit endophytes, which are beneficial to plant growth. In summary, our data show that the dynamics of rice root bacterial composition are mainly influenced by the plant growth stage. Our findings offer new insights regarding the control of the root microbiome by host plants and the influence of farming systems.

H2S action in plant life cycle

2021 ◽  
Author(s):  
Mingjian Zhou ◽  
Heng Zhou ◽  
Jie Shen ◽  
Zhirong Zhang ◽  
Cecilia Gotor ◽  
...  
Keyword(s):  
Life Cycle ◽  
Plant Life ◽  
Plant Life Cycle

scholarly journals Datenintegration im Plant Life Cycle - Schneller, effizienter Informationsfluss zwischen den an Entwicklung, Bau und Betrieb einer Anlage Beteiligten

2012 ◽  
Vol 84 (8) ◽  
pp. 1226-1226
Author(s):  
J. S. Kussi ◽  
H. Richert ◽  
U. P. Stramma ◽  
M. Rittmeister ◽  
S. Zeck
Keyword(s):  
Life Cycle ◽  
Plant Life ◽  
Plant Life Cycle

scholarly journals Here, there and everywhere – the importance of neutral lipids in plant growth and development

2021 ◽  
Author(s):  
Agnieszka Zienkiewicz ◽  
Marta Saldat ◽  
Krzysztof Zienkiewicz
Keyword(s):  
Life Cycle ◽  
Neutral Lipids ◽  
Single Layer ◽  
Pollen Grains ◽  
Dynamic Nature ◽  
Plant Life ◽  
Plant Environment ◽  
Plant Life Cycle ◽  
Diverse Plant

In plants, lipids serve as one of the major and vital cellular constituents. Neutral lipids reserves play an essential role in the plant life cycle by providing carbon and energy equivalents for periods of active metabolism. The most common form of lipid storage are triacylglycerols (TAGs) packed into specialized organelles called lipid droplets (LDs). They have been observed in diverse plant organs and tissues, like oil seeds or pollen grains. LDs consist of a core, composed mostly of TAGs, enclosed by a single layer of phospholipids that is decorated by a unique set of structural proteins. Moreover, the recent advances in exploration of LDs proteome revealed a plethora of diverse proteins interacting with LDs. This is likely the result of a highly dynamic nature of these organelles and their involvement in many diverse aspect of cellular metabolism, tightly synchronized with plant developmental programs and directly related to plant-environment interactions. In this review we summarize and discuss the current progress in understanding the role of LDs and their cargo during plants life cycle, with a special emphasis on developmental aspects.

scholarly journals Diversity Analysis of Endophytic Bacterial Community in Two Citrus Cultivars With Different Resistance to Citrus Canker

2021 ◽  
Author(s):  
Bing Liu ◽  
Jiahao Lai ◽  
Simeng Wu ◽  
Junxi Jiang ◽  
Weigang Kuang
Keyword(s):  
Bacterial Community ◽  
Endophytic Bacteria ◽  
Citrus Canker ◽  
Navel Orange ◽  
Xanthomonas Citri ◽  
Canker Disease ◽  
Satsuma Mandarin ◽  
Endophytic Bacterial Community ◽  
Dominant Bacteria ◽  
The Relationship

Abstract The selective infection of Xanthomonas citri pv. citri to citrus cultivars is universally known, but it is not clarified whether there is a relationship between endophytic bacteria and the resistance of host variety to canker disease. In order to explore the relationship, Satsuma mandarin and Newhall navel orange were collected respectively as samples of resistant or susceptible cultivars to citrus canker disease, and endophytic bacterial community of two citrus cultivars were analyzed by using a next-generation, Illumina-based sequencing approach. Simultaneously, the seasonal dynamics of endophytic bacterial community and dominant genera were analyzed. The results showed that there were four dominant groups including Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes in all samples at phylum level. Endophytic bacteria were the most abundant in spring samples, then in summer and autumn samples. There were some differences between endophytic bacterial community of resistant citrus and that of susceptible citrus to canker disease, and the endophytic bacteria of Satsuma mandarin are more abundant than that of Newhall navel orange. According to the analysis of dominant bacteria in two citrus cultivars, it was found that some endophytic bacteria with antagonistic characteristics existed universally in all samples, although the dominant bacteria in different seasonal sample were different. However, in Newhall navel orange of susceptible citrus to canker disease, there were not only some bacteria against Xanthomonas citri pv. citri, but also some cooperative bacteria of canker occurrence like Stenotrophomonas.

scholarly journals Root-Associated Endophytic Bacterial Community Composition of Pennisetum sinese from Four Representative Provinces in China

2019 ◽  
Vol 7 (2) ◽  
pp. 47 ◽  
Author(s):  
Zhen-Shan Deng ◽  
Bao-Cheng Zhang ◽  
Xiang-Ying Qi ◽  
Zhi-Hong Sun ◽  
Xiao-Long He ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Community Composition ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Bacterial Community Composition ◽  
Shannon Index ◽  
Plant Production ◽  
Total Carbon ◽  
Shaanxi Province ◽  
Endophytic Bacterial Community

Pennisetum sinese, a source of bio-energy with high biomass production, is a species that contains high crude protein and will be useful for solving the shortage of forage grass after the implementation of “Green for Grain” project in the Loess plateau of Northern Shaanxi in 1999. Plants may receive benefits from endophytic bacteria, such as the enhancement of plant growth or the reduction of plant stress. However, the composition of the endophytic bacterial community associated with the roots of P. sinese is poorly elucidated. In this study, P. sinese from five different samples (Shaanxi province, SX; Fujian province, FJ; the Xinjiang Uyghur autonomous prefecture, XJ and Inner Mongolia, including sand (NS) and saline-alkali land (NY), China) were investigated by high-throughput next-generation sequencing of the 16S rDNA V3-V4 hypervariable region of endophytic bacteria. A total of 313,044 effective sequences were obtained by sequencing five different samples, and 957 effective operational taxonomic units (OTUs) were yielded at 97% identity. The phylum Proteobacteria, the classes Gammaproteobacteria and Alphaproteobacteria, and the genera Pantoea, Pseudomonas, Burkholderia, Arthrobacter, Psychrobacter, and Neokomagataea were significantly dominant in the five samples. In addition, our results demonstrated that the Shaanxi province (SX) sample had the highest Shannon index values (3.795). We found that the SX (308.097) and NS (126.240) samples had the highest and lowest Chao1 richness estimator (Chao1) values, respectively. Venn graphs indicated that the five samples shared 39 common OTUs. Moreover, according to results of the canonical correlation analysis (CCA), soil total carbon, total nitrogen, effective phosphorus, and pH were the major contributing factors to the difference in the overall composition of the bacteria community in this study. Our data provide insights into the endophytic bacteria community composition and structure of roots associated with P. sinese. These results might be useful for growth promotion in different samples, and some of the strains may have the potential to improve plant production in future studies.

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