scholarly journals Quercetin and a plant substance, identified using a multi-copy number of DNA topoisomerase I in recombinant Pichia pastoris, inhibited MDA-MB-231 proliferation via different manners.

2021 ◽  
Author(s):  
Zhao Gang ◽  
Chao Sin Pei ◽  
Nur Adila Fadzil ◽  
Lim Shern Kwok ◽  
Chew Ai Lan ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Mrna Expression ◽  
Copy Number ◽  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Growth Inhibitors ◽  
Dna Topoisomerase ◽  
Cycle Arrest ◽  
Gene Mrna

Abstract The study employed an in vivo strategy to construct a multi-copy number of human DNA topoisomerase I (hTopI) gene using pPIC3.5K vector in GS115 strain of Pichia. The yeast transformant (GS115-pPIC3.5K-hTopI; clone) was then used to investigate the preliminary growth effect of a pure compound (quercetin) and a standardised subfraction of ethanolic red onion peel extract (F1). The clones’ cell density was likely to be unaffected; only the total protein expression and enzyme activity were increased following the increased copy number of hTop1 in the host. The clone that showed the target enzyme's highest activity is said to respond specifically to growth inhibitors, whereby both quercetin and F1 were proven to be potential growth inhibitors as assessed by the MTT assay. In the process, quercetin reduced cell proliferation by inducing apoptosis and cell cycle arrest (S phase only), whereas F1 reduced cell proliferation by inducing cell cycle arrest only (S and G2 phases). Quercetin and F1 induced CYP1A1 and CYP1B1 (carcinogenicity) gene mRNA expression, but only F1 induced CYP2S1 (cytotoxicity) gene mRNA expression in the treated cells, suggesting that both quercetin and F1 inhibited the cell proliferation of MDA-MB-231 via different manners. The newly developed GS115-pPIC3.5K-hTopI can be used to select various potential substances for breast cancer treatment in the future.

scholarly journals Quercetin and a plant substance, identified using a multi-copy number of DNA topoisomerase I in recombinant Pichia pastoris, inhibited MDA-MB-231 proliferation via different manners.

2021 ◽  
Author(s):  
Zhao Gang ◽  
Chao Sin Pei ◽  
Nur Adila Fadzil ◽  
Lim Shern Kwok ◽  
Chew Ai Lan ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Mrna Expression ◽  
Copy Number ◽  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Growth Inhibitors ◽  
Dna Topoisomerase ◽  
Cycle Arrest ◽  
Gene Mrna

Abstract The study aimed to employ an in vivo strategy to construct a multi-copy number of human DNA topoisomerase I (hTopI) gene using pPIC3.5K vector in GS115 strain of Pichia. The yeast transformant (GS115-pPIC3.5K-hTopI) was then used to investigate the preliminary growth effect of a pure compound (quercetin) and a standardised subfraction of ethanolic red onion peel extract (F1). The underlying mechanisms of quercetin and F1 were then tested on MDA-MB-231 for the cell cycle profile and apoptosis by flow cytometry, and the mRNA expression of CYP genes by real-time PCR. His + yeast transformants (clones) with multi-copy inserts resistant to various concentrations of Geneticin were successfully selected in the study. The clones’ cell density was likely to be unaffected; only the total protein expression and enzyme activity were increased following the increased copy number of hTop1 in the host. The clone that showed the target enzyme's highest activity is said to respond specifically to growth inhibitors, whereby both quercetin and F1 were proven to be potential growth inhibitors as assessed by the MTT assay. In the process, quercetin reduced cell proliferation by inducing apoptosis and cell cycle arrest (S phase only), whereas F1 reduced cell proliferation by inducing cell cycle arrest only (S and G2 phases). Quercetin and F1 induced CYP1A1 and CYP1B1 (carcinogenicity) gene mRNA expression, but only F1 induced CYP2S1 (cytotoxicity) gene mRNA expression in the treated cells, suggesting that both quercetin and F1 inhibited the cell proliferation of MDA-MB-231 via different manners. The newly developed GS115-pPIC3.5K-hTopI can be used to select various potential substances for breast cancer treatment in the future.

DNA topoisomerase I and II activities during cell proliferation and the cell cycle in cultured mouse embryo fibroblast (C3H 10T1/2) cells

1985 ◽  
Vol 158 (1) ◽  
pp. 1-14 ◽  
Author(s):  
James V. Tricoli ◽  
Beni M. Sahai ◽  
Patrick J. McCormick ◽  
Sandra J. Jarlinski ◽  
John S. Bertram ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Mouse Embryo ◽  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Mouse Embryo Fibroblast ◽  
Dna Topoisomerase

scholarly journals DNA Topoisomerase I Gene Copy Number and mRNA Expression Assessed as Predictive Biomarkers for Adjuvant Irinotecan in Stage II/III Colon Cancer

2015 ◽  
Vol 22 (7) ◽  
pp. 1621-1631 ◽  
Author(s):  
Sune Boris Nygård ◽  
Ben Vainer ◽  
Signe Lykke Nielsen ◽  
Fred Bosman ◽  
Sabine Tejpar ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Mrna Expression ◽  
Copy Number ◽  
Topoisomerase I ◽  
Gene Copy Number ◽  
Predictive Biomarkers ◽  
Gene Copy ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
I Gene

scholarly journals A Novel DNA Topoisomerase I Inhibitor with Different Mechanism from Camptothecin Induces G2/M Phase Cell Cycle Arrest to K562 Cells

Biochemistry ◽  
2010 ◽  
Vol 49 (47) ◽  
pp. 10131-10136 ◽  
Author(s):  
Ning Wu ◽  
Xi-Wei Wu ◽  
Keli Agama ◽  
Yves Pommier ◽  
Jun Du ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Topoisomerase I ◽  
K562 Cells ◽  
Phase Cell ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Topoisomerase I Inhibitor ◽  
Cycle Arrest ◽  
M Phase

DNA Damage Induced by DNA Topoisomerase I- and Topoisomerase II- Inhibitors Detected by Histone H2AX Phosphorylation in Leukemic Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4240-4240
Author(s):  
Dorota H. Halicka ◽  
Xuan Huang ◽  
Fevzi M. Ozkaynak ◽  
Karen Seiter ◽  
Frank Traganos ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Topoisomerase I ◽  
Caspase 3 ◽  
S Phase ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Dna Topoisomerase I ◽  
H2ax Phosphorylation ◽  
Dna Topoisomerase ◽  
Histone H2ax

Abstract Histone H2AX is phosphorylated on Ser-139 by ATM kinase in response to damage that induces dsDNA breaks. Immunocytochemical detection of phosphorylated H2AX (γH2AX), thus, reveals the presence of dsDNA breaks in chromatin. Multiparameter cytometry was presently used to correlate the appearance of γH2AX with: (a) cell cycle phase; (b) caspase-3 activation; and (c) apoptosis-associated DNA fragmentation in individual human leukemic HL-60 cells treated with the DNA topoisomerase I (topo1) inhibitors topotecan (TPT) and camptothecin (CPT) or with the topo2 inhibitor mitoxantrone (MTX). In response to TPT or CPT maximal increase of γH2AX immunofluorescence was seen in S-phase cells by 90 min. In contrast, following MTX treatment the maximal rise of γH2AX was detected at 2 h in G1 cells and the cell cycle phase specificity was much less apparent. A linear relationship between the drug concentration and increase of γH2AX immunofluorescence was seen only up to 200 nM TPT; a decline in γH2AX was apparent at a concentration range between 0.4 and 1.6 μM TPT. Thus, the intensity of γH2AX immunofluorescence, as a marker of cell survival following TPT treatment, can be used only within a limited range of drug concentration. Following treatment with TPT, CPT or MTX the peak of H2AX phosphorylation preceded caspase-3 activation and the appearance of apoptosis-associated DNA fragmentation, both selective to S-phase cells. Progression of apoptosis was paralleled by a decrease in γH2AX immunofluorescence. On the basis of our laboratory results, the present clinical study is evaluating ex vivo the feasibility of assessing DNA damage induced by treatment with topoisomerase inhibitors in patients with acute leukemias.

DNA Topoisomerase I and Cell Proliferation: Analysis of top1 Gene Expression in Different Carrot Tissues

1995 ◽  
Vol 129 (4) ◽  
pp. 933-934
Author(s):  
Alma Balestrazzi ◽  
Ilaria Toscano ◽  
Mei-Zhong Luo ◽  
Giovanni Bernacchia ◽  
Daniela Carbonera
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase

scholarly journals Induction of cell cycle arrest and apoptosis by copper complex Cu(SBCM)2 towards oestrogen-receptor positive MCF-7 breast cancer cells

RSC Advances ◽  
2019 ◽  
Vol 9 (32) ◽  
pp. 18359-18370 ◽  
Author(s):  
Jhi Biau Foo ◽  
Li Shan Ng ◽  
Ji Hui Lim ◽  
Pau Xien Tan ◽  
Yan Zhi Lor ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Topoisomerase I ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Dna Topoisomerase ◽  
Cycle Arrest ◽  
Mcf 7

Cu(SBCM)2 binds to DNA topoisomerase I, which, in turn, induces cell cycle arrest and apoptosis in MCF-7 breast cancer cells, possibly via p53 signalling pathway.

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