The Residue of Tetracycline Antibiotics in Soil and Vegetable and Its Effect on the Diversity of Soil Bacterial Community

Large Scale ◽

Chicken Manure ◽

Pig Manure ◽

Livestock Manure ◽

Tetracycline Antibiotics ◽

Soil Microbial ◽

Manure Treatment

Abstract [Background]Tetracycline antibiotics (TCs) are a broad-spectrum antibiotic, widely used in livestock and poultry breeding. The residue of tetracycline antibiotics in animal manure may cause changes of soil microbial community.[Methods]On the basis of the investigation and analysis of TCs pollution in the soil of main vegetable bases and the livestock manure of major large-scale farms in Chongqing, China, the effects of tetracycline antibiotics on the structure and diversity of soil microbial community were investigated by high-throughput sequencing.[Results]The TCs content in soil was increased by applying livestock manure. The contents of TC, OTC and CTC in the soil under pig manure treatment were 171.07-660.20 μg·kg-1, 25.38-345.78 μg·kg-1 and 170.77-707.47 μg·kg-1, respectively. The contents of TC, OTC and CTC in the soil under the treatment of chicken manure were 166.62-353.61 μg·kg-1, 122.25-251.23 μg·kg-1 and 15.12-80.91 μg·kg-1 respectively. The content of tetracycline antibiotics (TCs) in edible parts of Brassica juncea var. gemmifera was increased after livestock manure treatment, among which pig manure had the greatest influence on the increase of TCs content, and the larger the dosage of pig manure was, the more significant the increase of TCs content was. Proteobacteria, Acidobacteria, Actinobacteria, Chioroflexi and bacteroidetes under livestock manure treatment were the dominant phyla, accounting for 85.2%-92.4% of the total abundance of soil bacteria.[Conclusions]The soil OTUs under the treatment of pig manure was higher than that under the treatment of chicken manure. Biogas residue after fermentation treatment can effectively reduce the environmental and ecological risks caused by antibiotic residues.

The Residue of Tetracycline Antibiotics in Soil and Vegetable and Its Effect On The Diversity of Soil Bacterial Community

10.21203/rs.3.rs-92742/v1 ◽

2020 ◽

Author(s):

Longshui Xu ◽

Weizhong Wang ◽

Weihong Xu

Keyword(s):

Microbial Community ◽

Large Scale ◽

Chicken Manure ◽

Pig Manure ◽

Livestock Manure ◽

Tetracycline Antibiotics ◽

Soil Microbial ◽

Manure Treatment

Abstract [Background] Tetracycline antibiotics (TCs) are a broad-spectrum antibiotic, widely used in livestock and poultry breeding. The residue of tetracycline antibiotics in animal manure may cause changes of soil microbial community. [Methods] On the basis of the investigation and analysis of TCs pollution in the soil of main vegetable bases and the livestock manure of major large-scale farms in Chongqing, China, the effects of tetracycline antibiotics on the structure and diversity of soil microbial community were investigated by high-throughput sequencing. [Results] The TCs content in soil was increased by applying livestock manure. The contents of TC, OTC and CTC in the soil under pig manure treatment were 171.07-660.20 μg·kg-1, 25.38-345.78 μg·kg-1 and 170.77-707.47 μg·kg-1, respectively. The contents of TC, OTC and CTC in the soil under the treatment of chicken manure were 166.62-353.61 μg·kg-1, 122.25-251.23 μg·kg-1 and 15.12-80.91 μg·kg-1 respectively. The content of tetracycline antibiotics (TCs) in edible parts of Brassica juncea var. gemmifera was increased after livestock manure treatment, among which pig manure had the greatest influence on the increase of TCs content, and the larger the dosage of pig manure was, the more significant the increase of TCs content was. Proteobacteria, Acidobacteria, Actinobacteria, Chioroflexi and bacteroidetes under livestock manure treatment were the dominant phyla, accounting for 85.2%-92.4% of the total abundance of soil bacteria. [Conclusions] The soil OTUs under the treatment of pig manure was higher than that under the treatment of chicken manure. Biogas residue after fermentation treatment can effectively reduce the environmental and ecological risks caused by antibiotic residues.

Microbial Community Changes in the Rhizosphere Soil of Healthy and Rusty Panax ginseng and Discovery of Pivotal Fungal Genera Associated with Rusty Roots

BioMed Research International ◽

10.1155/2020/8018525 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13 ◽

Cited By ~ 4

Author(s):

Xuemin Wei ◽

Xiaoyue Wang ◽

Pei Cao ◽

Zitong Gao ◽

Amanda Juan Chen ◽

...

Keyword(s):

Microbial Community ◽

Panax Ginseng ◽

Fungal Pathogens ◽

Microbial Community Composition ◽

Pathogenic Fungi ◽

Linear Discriminant ◽

Rhizosphere Soils ◽

Soil Microbial

Panax ginseng Meyer, a valuable medicinal plant, is severely threatened by rusty root, a condition that greatly affects its yield and quality. Studies investigating the relationship between soil microbial community composition and rusty roots are vital for the production of high-quality ginseng. Here, high-throughput sequencing was employed to systematically characterize changes in the soil microbial community associated with rusty roots. Fungal diversity was lower in the soils of rusty root-affected P. ginseng than in those of healthy plants. Importantly, principal coordinate analysis separated the fungal communities in the rhizosphere soils of rusty root-affected ginseng from those of healthy plants. The dominant bacterial and fungal genera differed significantly between rhizosphere soils of healthy and rusty root-affected P. ginseng, and linear discriminant analysis effect size (LEfSe) further indicated a strong imbalance in the soil microbial community of diseased plants. Significantly enriched bacterial genera (including Rhodomicrobium, Knoellia, Nakamurella, Asticcacaulis, and Actinomadura) were mainly detected in the soil of rusty root-affected P. ginseng, whereas significantly enriched fungal genera (including Xenopolyscytalum, Arthrobotrys, Chalara, Cryptococcus, and Scutellinia) were primarily detected in the soil of healthy plants. Importantly, five fungal genera (Cylindrocarpon, Acrophialophora, Alternaria, Doratomyces, and Fusarium) were significantly enriched in the soil of rusty root-affected plants compared with that of healthy plants, suggesting that an increase in the relative abundance of these pathogenic fungi (Cylindrocarpon, Alternaria, and Fusarium) may be associated with ginseng rusty roots. Additionally, this study is the first to report that an increase in the relative abundances of Acrophialophora and Doratomyces in the rhizosphere of P. ginseng may be associated with the onset of rusty root symptoms in this plant. Our findings provide potentially useful information for developing biological control strategies against rusty root, as well as scope for future screening of fungal pathogens in rusty roots of P. ginseng.

Combined effects of chlortetracycline and dissolved organic matter extracted from pig manure on the functional diversity of soil microbial community

Soil Biology and Biochemistry ◽

10.1016/j.soilbio.2014.03.005 ◽

2014 ◽

Vol 74 ◽

pp. 148-155 ◽

Cited By ~ 37

Author(s):

Bei Liu ◽

Yanxia Li ◽

Xuelian Zhang ◽

Jing Wang ◽

Min Gao

Keyword(s):

Organic Matter ◽

Microbial Community ◽

Dissolved Organic Matter ◽

Functional Diversity ◽

Pig Manure ◽

Combined Effects ◽

Soil Microbial

α-Terpineol fumigation alleviates negative plant-soil feedbacks of Panax notoginseng via suppressing Ascomycota and enriching antagonistic bacteria

Phytopathology Research ◽

10.1186/s42483-021-00090-1 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Chen Ye ◽

Yixiang Liu ◽

Junxing Zhang ◽

Tianyao Li ◽

Yijie Zhang ◽

...

Keyword(s):

Microbial Community ◽

Relative Abundance ◽

Fungal Pathogens ◽

Panax Notoginseng ◽

Dependent Manner ◽

Beneficial Bacteria ◽

Soil Microbial ◽

Plant Soil

AbstractThe accumulation of soil-borne pathogens is the main driving factor of negative plant-soil feedbacks (NPSFs), which seriously restricts the sustainable development of agriculture. Using natural volatile organic compounds (VOCs) from plants or microorganisms as biofumigants is an emerging strategy to alleviate NPSFs in an environmentally-friendly way. Here, we identified α-terpineol from the VOCs of pine needles, confirmed the ability of α-terpineol fumigation in alleviating the NPSF of Panax notoginseng via significantly reducing seed decay rate, and also deciphered the underlying mechanism by which the soil microbial community is modified. α-Terpineol fumigation could suppress culturable fungi but enrich bacteria in a dose-dependent manner. Network analysis with high-throughput sequencing data revealed that α-terpineol could distinctly modify both fungal and bacterial communities. In detail, α-terpineol significantly suppressed the relative abundance of Ascomycota from 64.04 to 32.26%, but enriched the relative abundance of Proteobacteria, Acidobacteria and Actinobacteria. Subnetwork analysis further demonstrated that α-terpineol could directly or indirectly suppress fungal pathogens and enrich plant growth-promoting rhizobacteria (PGPRs). In vitro fumigation and co-culture experiments with culturable isolates validated these findings. The antagonism between beneficial bacteria and pathogens, and the synergistic growth promotion among α-terpineol-enriched bacteria might be involved in soil microbial community assembly. In summary, α-terpineol fumigation could directly or indirectly modify the soil microbial community to alleviate NPSFs, especially by suppressing fungal pathogens and enriching beneficial bacteria. This study suggests that VOCs from natural products are worth developing as biofumigants due to their multiple functions in modifying the soil microbial community.