A dual electro-optical biosensor based on Chlamydomonas reinhardtii immobilised on paper-based nanomodified screen-printed electrodes for herbicide monitoring

Abstract The indiscriminate use of herbicides in agriculture contributes to soil and water pollution, with important endangering consequences on the ecosystems. Among the available analytical systems, algal biosensors have demonstrated to be valid tools thanks to their high sensitivity, cost-effectiveness, and eco-design. Herein, we report the development of a dual electro-optical biosensor for herbicide monitoring, based on Chlamydomonas reinhardtii whole cells immobilised on paper-based screen-printed electrodes modified with carbon black nanomaterials. To this aim, a systematic study was performed for the selection and characterisation of a collection among 28 different genetic variants of the alga with difference response behaviour towards diverse herbicide classes. Thus, CC125 strain was exploited as case study for the study of the analytical parameters. The biosensor was tested in standard solutions and real samples, providing high sensitivity (detection limit in the pico/nanomolar), high repeatability (RSD of 5% with n = 100), long lasting working (10 h) and storage stability (3 weeks), any interference in the presence of heavy metals and insecticides, and low matrix effect in drinking water and moderate effect in surface one.

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A dual electro-optical biosensor based on Chlamydomonas reinhardtii immobilised on paper-based nanomodified screen-printed electrodes for herbicide monitoring

Journal of Nanobiotechnology ◽

10.1186/s12951-021-00887-4 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Amina Antonacci ◽

Raouia Attaallah ◽

Fabiana Arduini ◽

Aziz Amine ◽

Maria Teresa Giardi ◽

...

Keyword(s):

Chlamydomonas Reinhardtii ◽

High Sensitivity ◽

Optical Biosensor ◽

Whole Cells ◽

Screen Printed Electrodes ◽

And Storage ◽

High Repeatability ◽

High Sensitivity Detection ◽

Screen Printed

AbstractThe indiscriminate use of herbicides in agriculture contributes to soil and water pollution, with important endangering consequences on the ecosystems. Among the available analytical systems, algal biosensors have demonstrated to be valid tools thanks to their high sensitivity, cost-effectiveness, and eco-design. Herein, we report the development of a dual electro-optical biosensor for herbicide monitoring, based on Chlamydomonas reinhardtii whole cells immobilised on paper-based screen-printed electrodes modified with carbon black nanomaterials. To this aim, a systematic study was performed for the selection and characterisation of a collection among 28 different genetic variants of the alga with difference response behaviour towards diverse herbicide classes. Thus, CC125 strain was exploited as case study for the study of the analytical parameters. The biosensor was tested in standard solutions and real samples, providing high sensitivity (detection limit in the pico/nanomolar), high repeatability (RSD of 5% with n = 100), long lasting working (10 h) and storage stability (3 weeks), any interference in the presence of heavy metals and insecticides, and low matrix effect in drinking water and moderate effect in surface one.

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Nanocomposite based on restacked crystallites of β-NiS and Ppy for the determination of theophylline and uric acid on screen-printed electrodes

New Journal of Chemistry ◽

10.1039/c9nj04246f ◽

2019 ◽

Vol 43 (48) ◽

pp. 19397-19407

Author(s):

P. Muthukumaran ◽

R. Ramya ◽

P. Thivya ◽

J. Wilson ◽

G. Ravi

Keyword(s):

Uric Acid ◽

Electrocatalytic Activity ◽

High Sensitivity ◽

Screen Printed Electrodes ◽

Screen Printed

We synthesized calcinated β-NiS with a highly crystalline porous nature and mixed it with Ppy to prepare a nanocomposite, which exhibited high electrocatalytic activity and this was then used to detect theophylline and uric acid with high sensitivity and stability.

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Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose

Diagnostics ◽

10.3390/diagnostics10080517 ◽

2020 ◽

Vol 10 (8) ◽

pp. 517 ◽

Cited By ~ 3

Author(s):

Nicolae-Bogdan Mincu ◽

Veronica Lazar ◽

Dana Stan ◽

Carmen Marinela Mihailescu ◽

Rodica Iosub ◽

...

Keyword(s):

High Sensitivity ◽

Accurate Diagnosis ◽

Diagnostic Purpose ◽

Pathogenic Microorganisms ◽

Screen Printed Electrodes ◽

High Incidence ◽

In Vitro Diagnostic ◽

Sensitivity And Selectivity ◽

Screen Printed

Due to rapidly spreading infectious diseases and the high incidence of other diseases such as cancer or metabolic syndrome, there is a continuous need for the development of rapid and accurate diagnosis methods. Screen-printed electrodes-based biosensors have been reported to offer reliable results, with high sensitivity and selectivity and, in some cases, low detection limits. There are a series of materials (carbon, gold, platinum, etc.) used for the manufacturing of working electrodes. Each version comes with advantages, as well as challenges for their functionalization. Thus, the aim is to review the most promising biosensors developed using screen-printed electrodes for the detection/quantification of proteins, biomarkers, or pathogenic microorganisms.

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Graphene based electrochemical immunosensor for the ultra-sensitive label free detection of Alzheimer’s beta amyloid peptides Aβ(1-42)

Nanoscale Advances ◽

10.1039/d0na00801j ◽

2021 ◽

Author(s):

Hina Yaqub Abbasi ◽

Zari Tehrani ◽

Anitha Devadoss ◽

Muhammad Ali ◽

Soraya Moradi-Bachiller ◽

...

Keyword(s):

Alzheimer’S Disease ◽

High Sensitivity ◽

Beta Amyloid ◽

Electrochemical Immunosensor ◽

Label Free ◽

Amyloid Peptides ◽

Label Free Detection ◽

High Sensitivity Detection ◽

Sensitivity Detection ◽

Screen Printed

An immunosensor, capable of high sensitivity detection of beta-amyloid peptides, shown to be a reliable biomarker for Alzheimer’s disease, has been developed using screen printed graphene electrodes (SPGEs) modified with...

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Electrochemiluminescence Biosensors Using Screen-Printed Electrodes

Biosensors ◽

10.3390/bios10090118 ◽

2020 ◽

Vol 10 (9) ◽

pp. 118

Author(s):

Emiliano Martínez-Periñán ◽

Cristina Gutiérrez-Sánchez ◽

Tania García-Mendiola ◽

Encarnación Lorenzo

Keyword(s):

Dna Analysis ◽

Dynamic Range ◽

Chemical Properties ◽

High Sensitivity ◽

Portable Devices ◽

Research Fields ◽

Sensing Applications ◽

Screen Printed Electrodes ◽

Wide Range ◽

Screen Printed

Electrogenerated chemiluminescence (also called electrochemiluminescence (ECL)) has become a great focus of attention in different fields of analysis, mainly as a consequence of the potential remarkably high sensitivity and wide dynamic range. In the particular case of sensing applications, ECL biosensor unites the benefits of the high selectivity of biological recognition elements and the high sensitivity of ECL analysis methods. Hence, it is a powerful analytical device for sensitive detection of different analytes of interest in medical prognosis and diagnosis, food control and environment. These wide range of applications are increased by the introduction of screen-printed electrodes (SPEs). Disposable SPE-based biosensors cover the need to perform in-situ measurements with portable devices quickly and accurately. In this review, we sum up the latest biosensing applications and current progress on ECL bioanalysis combined with disposable SPEs in the field of bio affinity ECL sensors including immunosensors, DNA analysis and catalytic ECL sensors. Furthermore, the integration of nanomaterials with particular physical and chemical properties in the ECL biosensing systems has improved tremendously their sensitivity and overall performance, being one of the most appropriates research fields for the development of highly sensitive ECL biosensor devices.

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High-sensitivity detection of gold labels by high-angle dark-field STEM imaging

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162028 ◽

1990 ◽

Vol 48 (3) ◽

pp. 892-893 ◽

Cited By ~ 1

Author(s):

Max T. Otten

Keyword(s):

High Sensitivity ◽

Dark Field ◽

Bright Field ◽

Backscattered Electron Imaging ◽

Backscattered Electrons ◽

Average Atomic Number ◽

Highly Sensitive ◽

Backscattered Electron ◽

Electron Imaging ◽

High Sensitivity Detection

Labelling of antibodies with small gold probes is a highly sensitive technique for detecting specific molecules in biological tissue. Larger gold probes are usually well visible in TEM or STEM Bright-Field images of unstained specimens. In stained specimens, however, the contrast of the stain is frequently the same as that of the gold labels, making it virtually impossible to identify the labels, especially when smaller gold labels are used to increase the sensitivity of the immunolabelling technique. TEM or STEM Dark-Field images fare no better (Figs. 1a and 2a), again because of the absence of a clear contrast difference between gold labels and stain.Potentially much more useful is backscattered-electron imaging, since this will show differences in average atomic number which are sufficiently large between the metallic gold and the stains normally used. However, for the thin specimens and at high accelerating voltages of the STEM, the yield of backscattered electrons is very small, resulting in a very weak signal. Consequently, the backscattered-electron signal is often too noisy for detecting small labels, even for large spot sizes.

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Reservoir Characterization and Storage Capacity Evaluation for Carbon Capture and Storage: A Case Study From East Natuna

Proc of Indonesian Petroleum Association 42nd Annual Convention ◽

10.29118/ipa19.g.204 ◽

2018 ◽

Author(s):

K. Prabowo

Keyword(s):

Carbon Capture ◽

Reservoir Characterization ◽

Storage Capacity ◽

Carbon Capture And Storage ◽

Capacity Evaluation ◽

And Storage

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Facile and Fast Detection of Genistein in Derris scandens by Square Wave Voltammetry using a Cobalt(II) Phthalocyanine-Modified Screen-Printed Electrochemical Sensor

Current Analytical Chemistry ◽

10.2174/1573411014666180521091053 ◽

2020 ◽

Vol 16 (3) ◽

pp. 341-348

Author(s):

Surinya Traipop ◽

Suchada Chuanuwatanakul ◽

Orawon Chailapakul ◽

Eakkasit Punrat

Keyword(s):

Electrochemical Sensor ◽

Square Wave Voltammetry ◽

Silver Chloride ◽

Reference Electrode ◽

High Sensitivity ◽

Plastic Substrate ◽

Fast Analysis ◽

Square Wave ◽

Wave Voltammetry ◽

Screen Printed

Background: Recently, Derris scandens, a Thai herbal medicine with anti-inflammatory activity, is widely used as beverage and supplementary food. When the traditional medicine is a choice for health therapy, the simple and reliable equipment is required to control the suitable consuming amount of the active component. Objective: To develop the electrochemical sensor for genistein determination in Derris scandens with high sensitivity and rapid operation. Methods: An in-house screen-printed electrochemical sensor consisting of a three-electrode system was developed for genistein determination. A silver/silver chloride (Ag/AgCl) reference electrode, a carbon counter electrode and a carbon working electrode were prepared on a 0.3-mm-thick plastic substrate by the screen-printing technique using conductive ink. The dimensions of each sensor were 2.5×1.0 cm. Only 50 µL of sample solution was required on this device for the determination of genistein concentration by rapid response square wave voltammetry. Results: The oxidation peak of genistein appeared with good response in acidic media at a peak potential of 0.6 V. Moreover, the signal was enhanced by modifying the conductive carbon ink with cobalt( II) phthalocyanine. Under the optimized conditions, the linear range was found to be 2.5-150 µM and the detection limit was 1.5 µM. Moreover, the small volume extraction was successfully developed without any further pre-concentration. This proposed method was applied to determine genistein in Derris scandens with satisfying results. Conclusion: The proposed method is promising as an alternative method for genistein determination with facile and fast analysis.

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