scholarly journals Meta-QTL Analysis for Stripe Rust Resistance in Wheat

2021 ◽  
Author(s):  
Irfat Jan ◽  
Gautam Saripalli ◽  
Kuldeep Kumar ◽  
Anuj Kumar ◽  
Rakhi Singh ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Consensus Map ◽  
Sugar Transporters ◽  
Wheat Diseases ◽  
Genes Encoding ◽  
Genomic Regions

Abstract Stripe rust caused by Puccinia striiformis f. sp. tritici Eriks. & E. Henn (Pst) is one of the most prevalent wheat diseases causing upto 70% yield losses worldwide. The present study was conducted in wheat for the first time to identify important meta-QTL (MQTL) regions for their use in developing stripe rust resistant wheat cultivars and to understand the genetic architecture of stripe rust resistance in wheat. For this purpose, a dense consensus map consisting of 76,753 markers was constructed and 353 QTLs from earlier studies were projected on this consensus map. As many as 61 MQTLs were identified using 184 (out of 353) original QTLs. Ten important genomic regions including six breeders’ MQTLs (PVE >20%) and four MQTL hotspots were selected to be used by wheat breeders. As many as 409 important candidate genes (CGs) were also identified, which either encoded known R proteins (265) or showed differential expression (144) due to stripe rust infection. These included genes encoding the following proteins: NBS-LRR, WRKY domains, ankyrin repeat domains, sugar transporters, etc. Overall, the present study provided robust MQTLs and underlying CGs which may be potential targets for molecular breeding for development of stripe rust resistant wheat cultivars or may be the target for future molecular studies to understand the mechanism of stripe rust resistance.

scholarly journals Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Qingdong Zeng ◽  
Jianhui Wu ◽  
Shengjie Liu ◽  
Xianming Chen ◽  
Fengping Yuan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

scholarly journals Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars From the Huang-Huai-Hai Region of China

Plant Disease ◽  
2020 ◽  
Vol 104 (6) ◽  
pp. 1763-1770
Author(s):  
Liang Huang ◽  
Xing Zhi Xiao ◽  
Bo Liu ◽  
Li Gao ◽  
Guo Shu Gong ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Molecular Testing ◽  
Wheat Cultivars ◽  
Rust Resistance Genes ◽  
Yr Genes

Wheat stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious fungal disease worldwide, especially in the Huang-Huai-Hai region, a main wheat production area in China. Gene postulation, molecular testing, and pedigree analysis were conducted to determine the presence of stripe rust resistance genes to 15 Pst races in 66 selected commercial wheat cultivars released from 2000 to 2016. In addition, races CYR32, CYR33, and CYR34 were used to evaluate resistance to Pst at the adult-plant stage of wheat in the field. Four Yr genes (Yr9, Yr10, Yr26, and Yr32) were postulated in 24 wheat cultivars either singly or in combination. Thirty-six cultivars might contain unknown Yr genes, whereas no identified Yr gene was postulated in six cultivars. Yr9 was detected at a frequency of 28.8%, and no cultivars carried Yr5, Yr15, or Yr18. Ten cultivars (15.2%) exhibited adult-plant resistance in the field tests with three predominant races. Three cultivars (Langyan 43, Xinong 889, and Yunfeng 139) had all-stage resistance. These results are useful to growers selecting cultivars and to breeders aiming to use more resistance genes to develop new cultivars with effective resistance in order to reduce stripe rust damage.

Genome-wide Wheat 55K SNP-based Mapping of Stripe Rust Resistance Loci in Wheat Cultivar Shaannong 33 and Their Alleles Frequencies in Current Chinese Wheat Cultivars and Breeding Lines

Plant Disease ◽  
2020 ◽  
Author(s):  
Shuo Huang ◽  
Shengjie Liu ◽  
Yibo Zhang ◽  
Yanzhou Xie ◽  
Xiaoting Wang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Seedling Resistance ◽  
Resistant Parent ◽  
Breeding Lines ◽  
Chinese Wheat

Wheat cultivar Shannong 33 (SN33) has remained highly resistant to stripe rust in the field since its release in 2009. To unravel the genetic architecture of stripe rust resistance, seedlings of 161 recombinant inbred lines (RILs) from the cross Avocet S × SN33 were evaluated with two isolates (PST-Lab.1 and PST-Lab.2) of the stripe rust pathogen (Puccinia striiformis f. sp. tritici) in the greenhouse, and the RILs were evaluated in naturally and/or artificially inoculated field sites during two cropping seasons. The RILs and parents were genotyped with the wheat 55K single nucleotide polymorphism (SNP) array. Three genomic regions conferring seedling resistance were mapped on chromosomes 1DS, 2AS, and 3DS, and four consistent quantitative trait loci (QTL) for adult-plant resistance (APR) were detected on 1BL, 2AS, 3DL, and 6BS. The 2AS locus conferring all-stage resistance was identified as the resistant gene Yr17 located on 2NS translocation. The QTL identified on 1BL and 6BS likely correspond to Yr29 and Yr78, respectively. An APR QTL on 3DL explaining 5.8–12.2% of the phenotypic variation is likely to be new. Molecular marker detection assays with the 2NS segment (Yr17), Yr29, Yr78, and QYrsn.nwafu-3DL on a panel of 420 current Chinese wheat cultivars and breeding lines indicated that these genes were present in 11.4%, 7.6%, 14.8%, and 7.4% entries, respectively. The interactions among these genes/QTL were additive suggesting their potential value in enhancing stripe rust resistance breeding materials as observed in the resistant parent. In addition, we also identified two leaf necrosis genes, Ne1 and Ne2, however, the F1 plants from cross Avocet S × SN33 survived indicating that SN33 probably has another allele of Ne1 which allows to harvest seeds.

scholarly journals Evaluation of Stripe Rust Resistance in Hungarian Winter Wheat Cultivars in China

2019 ◽  
Vol 47 (4) ◽  
pp. 636-644
Author(s):  
D. Huang ◽  
H. Zhang ◽  
M. Tar ◽  
Y. Zhang ◽  
F. Ni ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Winter Wheat Cultivars

Distribution, Frequency and Variation of Stripe Rust Resistance Loci Yr10, Lr34/Yr18 and Yr36 in Chinese Wheat Cultivars

2012 ◽  
Vol 39 (11) ◽  
pp. 587-592 ◽  
Author(s):  
Cuiling Yuan ◽  
Hui Jiang ◽  
Honggang Wang ◽  
Kun Li ◽  
Heng Tang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Chinese Wheat ◽  
Distribution Frequency

scholarly journals Stripe rust resistance and genes in Chinese wheat cultivars and breeding lines

Euphytica ◽  
2013 ◽  
Vol 196 (2) ◽  
pp. 271-284 ◽  
Author(s):  
Qing-Dong Zeng ◽  
De-Jun Han ◽  
Qi-Lin Wang ◽  
Feng-Ping Yuan ◽  
Jian-Hui Wu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Chinese Wheat

Construction of a hexaploid wheat (Triticum aestivumL.) bacterial artificial chromosome library for cloning genes for stripe rust resistance

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 1028-1036 ◽  
Author(s):  
P Ling ◽  
X M Chen
Keyword(s):  
Triticum Aestivum ◽  
Stripe Rust ◽  
Hexaploid Wheat ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Stripe Rust Resistance ◽  
Insert Size ◽  
Bac Clones

A hexaploid wheat (Triticum aestivum L.) bacterial artificial chromosome (BAC) library was constructed for cloning Yr5 and other genes conferring resistance to stripe rust (Puccinia striiformis f. sp. tritici). Intact nuclei from a Yr5 near-isogenic line were used to isolate high molecular weight DNA, which was partially cleaved with HindIII and cloned into pECBAC1 and pIndigoBAC-5 vectors. The wheat BAC library consisted of 422 400 clones arrayed in 1100 micro-titer plates (each plate with 384 wells). Random sampling of 300 BAC clones indicated an average insert size of 140 kb, with a size range from 25 to 365 kb. Ninety percent of the clones in the library had an insert size greater than 100 kb and fewer than 5% of the clones did not contain inserts. Based on an estimated genome size of 15 966 Mb for hexaploid wheat, the BAC library was estimated to have a total coverage of 3.58× wheat genome equivalents, giving approximately 96% probability of identifying a clone representing any given wheat DNA sequence. Twelve BAC clones containing an Yr5 locus-specific marker (Yr5STS7/8) were successfully selected by PCR screening of 3-dimensional BAC pools. The results demonstrated that the T. aestivum BAC library is a valuable genomic resource for positional cloning of Yr5. The library also should be useful in cloning other genes for stripe rust resistance and other traits of interest in hexaploid wheat.Key words: BAC library, BAC pools, hexaploid wheat, Puccinia striiformis f. sp. tritici, resistance gene, stripe rust, Triticum aestivum.

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