scholarly journals Novel oxygen saturation imaging endoscopy to assess anastomotic integrity in a porcine ischemia model

2020 ◽  
Author(s):  
Hiro Hasegawa ◽  
Nobuyoshi Takeshita ◽  
Masaaki Ito

Abstract Background: Establishing anastomotic integrity is crucial for avoiding anastomotic complications in colorectal surgery. This study aimed to evaluate the safety and feasibility of assessing anastomotic integrity using novel oxygen saturation imaging endoscopy in a porcine ischemia model.Methods: In three pigs, a new endoscope system was used to check the mechanical completeness of the anastomosis and capture the tissue oxygen saturation (StO2) images. This technology can derive the StO2 images from the differences in the absorption coefficient in the visible light region between oxy- and deoxy-hemoglobin. Bowel perfusion at the proximal rectum was assessed before and after the anastomosis, and one minute and thirty minutes after the ligation of the cranial rectal artery (CRA).Results: The completeness of the anastomoses was confirmed by the absence of air leakage. Intraluminal oxygen saturation imaging was successfully performed in all animals. There was no significant difference in the StO2 level before and after the anastomosis (52.6 ± 2.0 vs. 52.0 ± 2.6; p = 0.76, respectively). The StO2 level of the intestine on the oral side of the anastomosis one minute after the CRA ligation was significantly lower than immediately after the anastomosis (15.9 ± 6.0 vs. 52.0 ± 2.6; p = 0.006, respectively). There was no significant difference in the StO2 level between one minute after and thirty minutes after the CRA ligation (15.9 ± 6.0 vs. 12.1 ± 5.3; p = 0.41, respectively).Conclusion: Novel oxygen saturation imaging endoscopy was safe and feasible to assess the anastomotic integrity in the experimental model.

2020 ◽  
Author(s):  
Hiro Hasegawa ◽  
Nobuyoshi Takeshita ◽  
Masaaki Ito

Abstract Background: Establishing anastomotic integrity is crucial for avoiding anastomotic complications in colorectal surgery. This study aimed to evaluate the safety and feasibility of assessing anastomotic integrity using novel oxygen saturation imaging endoscopy in a porcine ischemia model.Methods: In three pigs, a new endoscope system was used to check the mechanical completeness of the anastomosis and capture the tissue oxygen saturation (StO2) images. This technology can derive the StO2 images from the differences in the absorption coefficient in the visible light region between oxy- and deoxy-hemoglobin. Bowel perfusion at the proximal rectum was assessed before and after the anastomosis, and one minute and thirty minutes after the ligation of the cranial rectal artery (CRA).Results: The completeness of the anastomoses was confirmed by the absence of air leakage. Intraluminal oxygen saturation imaging was successfully performed in all animals. There was no significant difference in the StO2 level before and after the anastomosis (52.6 ± 2.0 vs. 52.0 ± 2.6; p = 0.76, respectively). The StO2 level of the intestine on the oral side of the anastomosis one minute after the CRA ligation was significantly lower than immediately after the anastomosis (15.9 ± 6.0 vs. 52.0 ± 2.6; p = 0.006, respectively). There was no significant difference in the StO2 level between one minute after and thirty minutes after the CRA ligation (15.9 ± 6.0 vs. 12.1 ± 5.3; p = 0.41, respectively).Conclusion: Novel oxygen saturation imaging endoscopy was safe and feasible to assess the anastomotic integrity in the experimental model.


BMC Surgery ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Hiro Hasegawa ◽  
Nobuyoshi Takeshita ◽  
Masaaki Ito

Abstract Background Establishing anastomotic integrity is crucial for avoiding anastomotic complications in colorectal surgery. This study aimed to evaluate the safety and feasibility of assessing anastomotic integrity using novel oxygen saturation imaging endoscopy in a porcine ischemia model. Methods In three pigs, a new endoscope system was used to check the mechanical completeness of the anastomosis and capture the tissue oxygen saturation (StO2) images. This technology can derive the StO2 images from the differences in the absorption coefficient in the visible light region between oxy- and deoxy-hemoglobin. Bowel perfusion at the proximal rectum was assessed before and after the anastomosis, and 1 min and 30 min after the ligation of the cranial rectal artery (CRA). Results The completeness of the anastomoses was confirmed by the absence of air leakage. Intraluminal oxygen saturation imaging was successfully performed in all animals. There was no significant difference in the StO2 level before and after the anastomosis (52.6 ± 2.0 vs. 52.0 ± 2.6; p = 0.76, respectively). The StO2 level of the intestine on the oral side of the anastomosis one minute after the CRA ligation was significantly lower than immediately after the anastomosis (15.9 ± 6.0 vs. 52.0 ± 2.6; p = 0.006, respectively). There was no significant difference in the StO2 level between 1 min after and 30 min after the CRA ligation (15.9 ± 6.0 vs. 12.1 ± 5.3; p = 0.41, respectively). Conclusion Novel oxygen saturation imaging endoscopy was safe and feasible to assess the anastomotic integrity in the experimental model.


2020 ◽  
Author(s):  
Hiro Hasegawa ◽  
Nobuyoshi Takeshita ◽  
Masaaki Ito

Abstract Background: Establishing anastomotic integrity is crucial for avoiding anastomotic complications in colorectal surgery. This study aimed to evaluate the safety and feasibility of assessing anastomotic integrity using novel oxygen saturation imaging endoscopy in a porcine ischemia model.Methods: In three pigs, a new endoscope system was used to check the mechanical completeness of the anastomosis and capture the tissue oxygen saturation (StO2) images. This technology can derive the StO2 images from the differences in the absorption coefficient in the visible light region between oxy- and deoxy-hemoglobin. Bowel perfusion at the proximal rectum was assessed before and after the anastomosis, and one minute and thirty minutes after the ligation of the cranial rectal artery (CRA).Results: The completeness of the anastomoses was confirmed by the absence of air leakage. Intraluminal oxygen saturation imaging was successfully performed in all animals. There was no significant difference in the StO2 level before and after the anastomosis (52.6 ± 2.0 vs. 52.0 ± 2.6; p = 0.76, respectively). The StO2 level of the intestine on the oral side of the anastomosis one minute after the CRA ligation was significantly lower than immediately after the anastomosis (15.9 ± 6.0 vs. 52.0 ± 2.6; p = 0.006, respectively). There was no significant difference in the StO2 level between one minute after and thirty minutes after the CRA ligation (15.9 ± 6.0 vs. 12.1 ± 5.3; p = 0.41, respectively).Conclusion: Novel oxygen saturation imaging endoscopy was safe and feasible to assess the anastomotic integrity in the experimental model.


2020 ◽  
Author(s):  
Hiro Hasegawa ◽  
Nobuyoshi Takeshita ◽  
Masaaki Ito

Abstract Background: Establishing anastomotic integrity is crucial for avoiding anastomotic complications in colorectal surgery. This study aimed to evaluate the safety and feasibility of assessing anastomotic integrity using novel oxygen saturation imaging endoscopy in a porcine ischemia model.Methods: In three pigs, a new endoscope system was used to check the mechanical completeness of the anastomosis and capture the tissue oxygen saturation (StO2) images. This technology can derive the StO2 images from the differences in the absorption coefficient in the visible light region between oxy- and deoxy-hemoglobin. Bowel perfusion at the proximal rectum was assessed before and after the anastomosis, and one minute and thirty minutes after the ligation of the cranial rectal artery (CRA).Results: The completeness of the anastomoses was confirmed by the absence of air leakage. Intraluminal oxygen saturation imaging was successfully performed in all animals. There was no significant difference in the StO2 level before and after the anastomosis (52.6 ± 2.0 vs. 52.0 ± 2.6; p = 0.76, respectively). The StO2 level of the intestine on the oral side of the anastomosis one minute after the CRA ligation was significantly lower than immediately after the anastomosis (15.9 ± 6.0 vs. 52.0 ± 2.6; p = 0.006, respectively). There was no significant difference in the StO2 level between one minute after and thirty minutes after the CRA ligation (15.9 ± 6.0 vs. 12.1 ± 5.3; p = 0.41, respectively).Conclusion: Novel oxygen saturation imaging endoscopy was safe and feasible to assess the anastomotic integrity in the experimental model.


2021 ◽  
Vol 11 (4) ◽  
pp. 55-61
Author(s):  
Rajesh Prabhakar Gaikwad ◽  
Akshaya Bhupesh Banodkar ◽  
Vaibhavi Pandurang Nandgaonkar

Background: Outbreak of novel corona virus has led World Health Organization (WHO) to come up with healthcare guidelines which includes wearing of N95 mask. The aim of this study was to evaluate whether blood oxygen saturation level is affected by wearing of N95 mask for the duration of 4 hours. Materials and Methods: A total of 90 exam going students were included in this study, who were instructed to wear N95 mask. Blood oxygen saturation level (SpO2) was checked both before and after wearing of N95 mask for a period of 4 hours. Results: It was observed that, before wearing N95 mask at 9 am, SpO2 values were 96.64±0.196 while at 1 pm after removing N95 mask, the saturation values were 95.68±0.235 (p=0.000) among all the students. There was a statistically highly significant difference seen for the values between the time intervals (p<0.01) with higher values at 9 am and lesser at 1pm. On comparing the SpO2 levels among males and females, a statistically nonsignificant difference was seen. Thus, reduction in blood oxygen saturation level can be a major parameter to evaluate the respiratory consequences of N95 mask. Conclusion: Various adverse effects of N95 mask were quoted in literature but respiratory consequences due to reduction in blood oxygen saturation level can be harmful. Thus, to avoid such consequences, precautionary measures are advised to be taken. Key words: N95 mask, SpO2, Blood oxygen saturation, COVID-19.


2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Aldo Calcante ◽  
Francesco M. Tangorra

The present study was aimed to measure the haemoglobin oxygen saturation and the pulse rate at teat level on dairy cows after and before milking, using a low cost pulse oximeter developed especially. The pulse oximeter has been tested during a three days of field test involving 18 Holstein Friesian cows raised in a commercial farm located in Northern Italy. The results highlighted that there is a significant difference both in haemoglobin oxygen saturation (SpO2) and pulse rate before and after milking considering the entire sample of animals. By dividing the sample according to the milking time (fast < 8 min and slow > 8 min), a significant difference between fast and slow cows has been observed for SpO2, whilst no difference has been noted considering the lactation stage (< 70 DIM and 71-140 DIM). About the pulse rate, on the contrary, milking time and lactation stage were not significantly different. This confirms that machine milking can create stress to the teat evoking circulatory impairment of its tissue and that pulse oximetry could be useful for detecting machine milking-induced alterations of teats. In perspective, the pulse oximeter could be used as a part of a monitoring system of the milking machine, enabling to change its operating parameters in order to minimize the mechanical stress on the teats.


2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Brenda N. E. Pelealu ◽  
Maya E.W. Moningka ◽  
Jimmy F. Rumampuk

Abstract: During physical activity, blood flow and oxygen usage will increase in order to transport oxygen which is required by muscles during exercise. One of the parameters to check the amount of oxygen that is bound to hemoglobin is oxygen saturation. This study aimed to determine the level of blood oxygen saturation of those who consumed mineral water and high oxygenated water during zumba gymnastics. This was an analytical experiments method with pre and post test approach. Total samples were 31 people who met the criteria and were willing to become the respondents. Data were analyzed using Wilcoxon Test. The results showed that the oxygen saturation before and after consuming mineral water (97.5±0.9) and (97.7±1.03) meanwhile before and after consuming high oxygenated water (97.6±0.75) and (98.7±0.44). The Wilcoxon test showed that there was a significant difference of blood oxygen saturation between before and after consuming high-oxygenated water (p=0.000) whereas for other respondents who consumed mineral water, there was no significant difference in their condition before and after consuming mineral water (p=0.198). Conclusion: There was a higher increase in blood oxygen saturation value before and after consuming oxygenated water compared to before and after consuming mineral water.Keywords: oxygen saturation, mineral water, and oxygenated water.Abstrak: Ketika beraktivitas fisik, aliran darah dan penggunaan oksigen akan meningkat untuk mengangkut oksigen yang diperlukan pada otot selama latihan. Salah satu parameter untuk melihat kadar oksigen yang berikatan dengan hemoglobin adalah Saturasi Oksigen. Penelitian ini bertujuan untuk mengetahui nilai saturasi oksigen darah pada orang yang mengkonsumsi air mineral dan air beroksigen tinggi pada senam zumba. Penelitian ini menggunakan metode analitik eksperimental dengan pendekatan pre and post test. Sampel penelitian berjumlah 31 orang yang memenuhi kriteria dan bersedia menjadi responden. Analisis data dilakukan dengan menggunakan uji Wilcoxon test. Hasil penelitian menunjukkan bahwa saturasi oksigen sebelum dan sesudah mengonsumsi air mineral (97,5±0,9) dan (97,7±1,03) sedangkan sebelum dan sesudah mengonsumsi air beroksigen tinggi (97,6±0,75) dan (98,7±0,44). Hasil uji Wilcoxon test menunjukkan perbedaan yang signifikan pada responden sebelum dan sesudah mengonsumsi air beroksigen (p=0,000) sedangkan ketika responden mengonsumsi air mineral terdapat perbedaan yang tidak signifikan pada kondisi sebelum dan sesudah mengkonsumsi air mineral (p=0,198). Simpulan: Terjadi peningkatan yang lebih tinggi dalam nilai saturasi oksigen darah sebelum dan sesudah mengkonsumsi air beroksigen dibandingkan dengan air mineral.Kata kunci: saturasi oksigen, air mineral, air beroksigen.


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