Virucidal activity of the antiseptic mouthwash and dental gel containing anionic phthalocyanine derivative: in vitro study

Abstract Aim: This research suggested an in vitro virucidal action of a dental gel and a mouthwash with phthalocyanine derivative.Purpose: The aim of this study was to report an in vitro study evaluating the virucidal capacity of mouthwash and dental gel containing anionic phtalocyanine derivate (APD).Methods: The research followed the recommendations of the National Health Surveillance Agency (ANVISA) and adapted methodology, described in the standards EN14776: 2015; ASTM E1053-11 and the Robert Koch Institute - RKI, in addition to Good Laboratory Practices (GLP). The determination of the percentage of inactivation of the SARS-CoV-2 virus particles was carried out by imposing the viral solution in contact with the respective tested products, with intervals of 30 seconds, 1 and 5 minutes, with subsequent submission of the aliquots, recovered in cell culture microplates following virus titration using the TCID50 (50% Median Tissue Culture Infectious Dose).Results: The Mouthwash APD presented 90% of viral inactivation percentage while the dental gel APD demonstrated 99.99% of viral inactivation.Conclusion: In vitro analyzes showed that mouthwash and dental gel APD can reduce the viability of SARS-CoV-2 virus particles.

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Determination of the maximum inhibitory dilution of cetylpyridinium chloride-based mouthwashes against staphylococcus aureus: an in vitro study

Journal of Applied Oral Science ◽

10.1590/s1678-77572008000400009 ◽

2008 ◽

Vol 16 (4) ◽

pp. 275-279 ◽

Cited By ~ 15

Author(s):

Evandro Watanabe ◽

Juliane Maria Guerreiro Tanomaru ◽

Andresa Piacezzi Nascimento ◽

Fumio Matoba-Júnior ◽

Mario Tanomaru-Filho ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Cetylpyridinium Chloride ◽

In Vitro Study ◽

Vitro Study

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Comparing the accuracy of two electronic apex locators in the determination of working length and the detection of root perforations: An in vitro study

Dental, Oral and Maxillofacial Research ◽

10.15761/docr.1000301 ◽

2019 ◽

Vol 5 (3) ◽

Author(s):

Kaveh Nasiri ◽

Karl-Thomas Wrbas

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Working Length

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Determination of coefficient of friction between the equine foot and different ground surfaces: an in vitro study

Equine and Comparative Exercise Physiology ◽

10.1017/s1478061506617234 ◽

2006 ◽

Vol 3 (4) ◽

pp. 191-198 ◽

Cited By ~ 8

Author(s):

Nicolas J Vos ◽

Dirk J Riemersma

Keyword(s):

Coefficient Of Friction ◽

Force Plate ◽

Ground Surface ◽

In Vitro Study ◽

Gravity Force ◽

Uniform Motion ◽

The Coefficient Of Friction ◽

Equine Industry

AbstractSlippery surfaces are a continuous concern in equine veterinary practice during both treatment and orthopaedic work-ups, especially when horses have to trot on circles. Sliding of the equine foot on the ground with the potential of injury is prevented if the horizontally acting accelerating or decelerating forces on the foot do not exceed maximal friction. Friction can be calculated and therefore anticipated if the coefficient of friction (μ) between the foot of the horse and the particular ground surface is known. Friction between shod and unshod cadaver equine hooves and different ground surfaces (concrete, tarmac and rubber) was determined by pulling the hooves horizontally in a uniform motion. Horizontal forces (Fh) were measured on a force plate and with a portable digital electronic force meter. The coefficient of friction (μ) was calculated as the quotient between Fh and the gravity force (N) of the object, hence: μ = Fh /N. This study has shown that the coefficient of friction between equine hooves and a specific ground surface can be determined using a portable digital force meter or a force plate. Friction significantly depended not only on the type of surface but also on shoeing of the equine foot. Bare feet showed more friction with the hard surfaces (bricks and tarmac), the shod feet showing more friction with the rubber surfaces. Coefficients of friction could be used to estimate the possibility of injuries occurring in the equine industry during exercise and/or lameness or pre-purchase examinations.

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Optimized Hepatitis E Virus (HEV) Culture and Its Application to Measurements of HEV Infectivity

Viruses ◽

10.3390/v12020139 ◽

2020 ◽

Vol 12 (2) ◽

pp. 139 ◽

Cited By ~ 2

Author(s):

Nicolas Capelli ◽

Martine Dubois ◽

Mélanie Pucelle ◽

Isabelle Da Silva ◽

Sébastien Lhomme ◽

...

Keyword(s):

Hepatitis E Virus ◽

Fetal Bovine Serum ◽

Hepatitis E ◽

Chronic Liver Diseases ◽

Infectious Dose ◽

Clinical Strains ◽

Fetal Bovine ◽

Culture Supernatants ◽

Tissue Culture Infectious Dose

Hepatitis E virus (HEV) is a major concern in public health worldwide. Infections with HEV genotypes 3, 4, or 7 can lead to chronic hepatitis while genotype 1 infections can trigger severe hepatitis in pregnant women. Infections with all genotypes can worsen chronic liver diseases. As virions are lipid-associated in blood and naked in feces, efficient methods of propagating HEV clinical strains in vitro and evaluating the infectivity of both HEV forms are needed. We evaluated the spread of clinical strains of HEV genotypes 1 (HEV1) and 3 (HEV3) by quantifying viral RNA in culture supernatants and cell lysates. Infectivity was determined by endpoint dilution and calculation of the tissue culture infectious dose 50 (TCID50). An enhanced HEV production could be obtained varying the composition of the medium, including fetal bovine serum (FBS) and dimethylsulfoxide (DMSO) content. This increased TCID50 from 10 to 100-fold and allowed us to quantify HEV1 infectivity. These optimized methods for propagating and measuring HEV infectivity could be applied to health safety processes and will be useful for testing new antiviral drugs.

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Determination of the reliability and repeatability of a quantitative occlusal analyzer by using a piezoelectric film sensor: An in vitro study

Journal of Prosthetic Dentistry ◽

10.1016/j.prosdent.2020.07.024 ◽

2020 ◽

Author(s):

Wonsup Lee ◽

Ho-Beom Kwon ◽

Myung-Joo Kim ◽

Young-Jun Lim

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Piezoelectric Film ◽

Film Sensor

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DEVELOPMENT OF A SPECIFIC RADIODMMUNOASSAY FOR DETERMINATION OF PEPTIDES DERIVED FROM HUMAN LEUKOCYTE ELASTASE DEGRADATION OF HUMAN FIBRIN (OGEN)

10.1055/s-0038-1643899 ◽

1987 ◽

Author(s):

R Wallin ◽

T Saldeen

Keyword(s):

Human Leukocyte ◽

In Vitro Study ◽

Molecular Size ◽

Molar Ratio ◽

Leukocyte Elastase ◽

Severe Renal Failure ◽

Human Leukocyte Elastase ◽

Edta Plasma

This paper describes a RIA for determination of the vasoactive peptide BB 30-43 and related peptides derived from leukocyte elastase degradaticn of human fibrin(ogen). The peptide was synthesized and could easily be labelled with 125I.Rabbits were iirmunized with BB 30-43 conjugated to bovine albumin. The antibody was found to bind about 5C% of the tracer in absence of BB 30-43 in a ˜1/800 diluticn. The RIA can detect peptide concentrations between 50 - 25000 pmol/L. The crossreaction with fibrinogen is very low (<0.001%) and with plas-min derived fibrin(ogen) peptides Bβ 1-42 and BB 15-42 also low (<0.2%). Plasma samples can be analyzed without any pretreatment. In an in vitro study fibrin and fibrincgen was degraded with plasmin or leukocyte elastase. Plasmin degradaticn of fibrin and fibrincgen did not release peptides which cross-reacted with our antibody, whereas leukocyte elastase degradation released peptides from both fibrin and fibrinogen which crossreact whith the antibody.The imnunolqgical activity was not changed after degrading peptide Bβ 30-43 with a) trypsin, b) plasmin, c) batraxobin, d) thrombin, e) elastase, at +37°,1 h, in a molar ratio of 1:100. Even degradaticn by elastase (1:3.5) +37°, 1 h, did not destroy the iirmunological activity.The imriunolcgical stability of peptide B< 30-43 in EDTA-plasma (+37°) seems to be very good. In citrated and heparinized plasma the activity of this peptide seems to vanish quite fast. In spite of these results we have detected high levels of iirmunolcgical activitiy in citrated or heparinized patient plasma. The molecular distribution of the peptides detected in plasma by our RIA corresponded to a fragnent containing about 25 amino acids. This fragnent seemes to be rather stable in plasma. When this fragnent was degraded with elastase in vitro a peptide with a molecular size resembling BB 30-43 was obtained. Over 300 patient samples have been studied. About 20 per cent were positive and the highest levels were found in patients with ARDS, septicaemia, severe renal failure, pulmonary embolism, pneumonia and pulmonary congestion.

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