Genetic Analysis and Gene Mapping of Two Whitebacked Planthopper Resistance Genes From Rice Varieties

Abstract Background The whitebacked planthopper (WBPH) has become a significant threat to rice production. Identification of WBPH-resistant germplasm and genes can drive efforts to develop resistance varieties and effectively limit pest damage.Methods Fourteen varieties of rice were surveyed for insect resistance using tests that assessed bulk seedling growth rates, insect feeding activity (via measurements of honeydew excretion weight), and insect development (by counting the number of hatched nymphs). Two resistance varieties N22 and OB677 were crossed with susceptible line 9311 to develop mapping populations, which were applied to map the resistance genes/QTLs.Results The rice variety PTB33 showed high resistance to both brown planthopper (BPH) and WBPH, and varieties N22, RBPH327, and OB677 showed moderate resistance to WBPH. Host choice test and seedling survival rates further verified the WBPH resistance of PTB33, N22, and OB677. By using two F2 mapping populations, two major resistance genes were detected in N22 and OB677. Wbph1 was mapped on chromosome 2 of N22 in a region that harbored the markers RM13650 and RM13478. Its largest logarithm of the odds (LOD) score was 3.94, which explained a 16.6% phenotypic variation. Wbph9 was mapped on chromosome 3 of OB677, where it was flanked by markers RM3513 and RM3525. It had a LOD score of 3.4, explaining a 17.2% phenotypic variation.Conclusions Four varieties PTB33, N22, RBPH327, and OB677 showed resistance to WBPH, of which OB677 was a novel resistance germplasm; and a novel resistance gene Wbph9 was mapped on chromosome 3.

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Mapping of Resistance Genes to Brown Planthopper in Untup Rajab, an Indonesian Local Rice Variety

Jurnal AgroBiogen ◽

10.21082/jbio.v14n2.2018.p75-84 ◽

2018 ◽

Vol 14 (2) ◽

pp. 75

Author(s):

Muhamad Yunus ◽

Diani Damayanti ◽

Ahmad Dadang ◽

Ahmad Warsun ◽

Dani Satyawan ◽

...

Keyword(s):

Resistance Genes ◽

Quantitative Resistance ◽

Insect Pest ◽

Rice Variety ◽

Brown Planthopper ◽

Snp Markers ◽

Chromosome 8 ◽

F2 Population ◽

Resistant Varieties ◽

Test Population

<p>Brown planthopper (BPH) is a major rice pest in Indonesia. The most economical and effective approach to control the insect pest is by using resistant varieties. Exploring for resistance genes is, therefore, a prerequisite for effective breeding program for BPH resistance. This study aimed to map BPH resistance genes in Untup Rajab, an Indonesian local rice variety. Genetic map was constructed using an F2 population from a cross between TN-1 and Untup Rajab, and SNP markers from RiceLD SNP Chip. Phenotyping was performed using bulk seedling test on F2:3 seedlings against two BPH populations, i.e. X1 and S1. Four QTLs<br />were identified on chromosomes 5, 6, 8, and 11 with PVE values of 7.63%, 9.40%, 17.66%, and 3.05%, respectively. Relatively normal distribution of resistance phenotype and the relatively low PVE values indicate that Untup Rajab has a quantitative resistance to BPH with two different resistance loci identified for each BPH test population. The QTL on chromosome 8 overlaps with OsHI-LOX gene, which is associated with resistance to BPH, and adjacent to another QTL for resistance to green leafhopper. The QTL on chromosome 6 was found near OsPLDα4 and OsPLDα5 genes which are related to BPH resistance. Meanwhile, the QTL intervals on chromosome 5 and 11 did not overlap with any known BPH QTLs or genes, which make them attractive candidates for novel BPH resistance gene discovery.</p>

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Two whitebacked planthopper resistance genes in rice share the same loci with those for brown planthopper resistance

Heredity ◽

10.1038/sj.hdy.6800398 ◽

2003 ◽

Vol 92 (3) ◽

pp. 212-217 ◽

Cited By ~ 47

Author(s):

G X Tan ◽

Q M Weng ◽

X Ren ◽

Z Huang ◽

L L Zhu ◽

...

Keyword(s):

Resistance Genes ◽

Brown Planthopper ◽

Brown Planthopper Resistance ◽

Whitebacked Planthopper

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Substitution mapping and characterization of brown planthopper resistance genes from indica rice variety, ‘PTB33’ (Oryza sativa L.)

Breeding Science ◽

10.1270/jsbbs.21034 ◽

2021 ◽

Author(s):

Cuong Dinh Nguyen ◽

Shao-Hui Zheng ◽

Sachiyo Sanada-Morimura ◽

Masaya Matsumura ◽

Hideshi Yasui ◽

...

Keyword(s):

Oryza Sativa ◽

Resistance Genes ◽

Rice Variety ◽

Brown Planthopper ◽

Substitution Mapping ◽

Brown Planthopper Resistance

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The Development and Characterization of Near-Isogenic and Pyramided Lines Carrying Resistance Genes to Brown Planthopper with the Genetic Background of Japonica Rice (Oryza sativa L.)

Plants ◽

10.3390/plants8110498 ◽

2019 ◽

Vol 8 (11) ◽

pp. 498

Author(s):

Cuong D. Nguyen ◽

Holden Verdeprado ◽

Demeter Zita ◽

Sachiyo Sanada-Morimura ◽

Masaya Matsumura ◽

...

Keyword(s):

Oryza Sativa ◽

Resistance Genes ◽

Genetic Background ◽

Oryza Sativa L ◽

Rice Variety ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Japonica Rice ◽

Rice Varieties ◽

Nilaparvata Lugens Stål

The brown planthopper (BPH: Nilaparvata lugens Stål.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice’s resistance to BPH in the context of regionally increasing levels of virulence.

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Development of InDel markers of Bph3 and pyramiding of four brown planthopper resistance genes into an elite rice variety

Molecular Breeding ◽

10.1007/s11032-020-01175-z ◽

2020 ◽

Vol 40 (10) ◽

Author(s):

Long He ◽

Ling Zou ◽

Qiuli Huang ◽

Xichen Sheng ◽

Weiren Wu ◽

...

Keyword(s):

Resistance Genes ◽

Rice Variety ◽

Brown Planthopper ◽

Indel Markers ◽

Brown Planthopper Resistance

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Mapping and breeding application of the brown planthopper ( Nilaparvata lugens )‐resistance genes derived from a durable resistant PTB33 rice variety ( Oryza sativa )

Plant Breeding ◽

10.1111/pbr.12980 ◽

2021 ◽

Author(s):

Ling Cheng ◽

Ya Zhu ◽

Jingying Li ◽

Zhe Jiang ◽

Wan Shu ◽

...

Keyword(s):

Oryza Sativa ◽

Resistance Genes ◽

Rice Variety ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Breeding Application

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The Impact of Climate Change on the Resistance of Rice Near-Isogenic Lines with Resistance Genes Against Brown Planthopper

Rice ◽

10.1186/s12284-021-00508-6 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Yun-Hung Kuang ◽

Yu-Fu Fang ◽

Shau-Ching Lin ◽

Shin-Fu Tsai ◽

Zhi-Wei Yang ◽

...

Keyword(s):

Climate Change ◽

Resistance Genes ◽

Environmental Changes ◽

Gene Pyramiding ◽

Brown Planthopper ◽

Inhibitory Effect ◽

Near Isogenic Lines ◽

Impact Of Climate Change ◽

Isogenic Lines ◽

The Impact

Abstract Background The impact of climate change on insect resistance genes is elusive. Hence, we investigated the responses of rice near-isogenic lines (NILs) that carry resistance genes against brown planthopper (BPH) under different environmental conditions. Results We tested these NILs under three environmental settings (the atmospheric temperature with corresponding carbon dioxide at the ambient, year 2050 and year 2100) based on the Intergovernmental Panel on Climate Change prediction. Comparing between different environments, two of nine NILs that carried a single BPH-resistant gene maintained their resistance under the environmental changes, whereas two of three NILs showed gene pyramiding with two maintained BPH resistance genes despite the environmental changes. In addition, two NILs (NIL-BPH17 and NIL-BPH20) were examined in their antibiosis and antixenosis effects under these environmental changes. BPH showed different responses to these two NILs, where the inhibitory effect of NIL-BPH17 on the BPH growth and development was unaffected, while NIL-BPH20 may have lost its resistance during the environmental changes. Conclusion Our results indicate that BPH resistance genes could be affected by climate change. NIL-BPH17 has a strong inhibitory effect on BPH feeding on phloem and would be unaffected by environmental changes, while NIL-BPH20 would lose its ability during the environmental changes.

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