Mapping and breeding application of the brown planthopper ( Nilaparvata lugens )‐resistance genes derived from a durable resistant PTB33 rice variety ( Oryza sativa )

2021 ◽  
Author(s):  
Ling Cheng ◽  
Ya Zhu ◽  
Jingying Li ◽  
Zhe Jiang ◽  
Wan Shu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Genes ◽  
Rice Variety ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Breeding Application

scholarly journals The Development and Characterization of Near-Isogenic and Pyramided Lines Carrying Resistance Genes to Brown Planthopper with the Genetic Background of Japonica Rice (Oryza sativa L.)

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 498
Author(s):  
Cuong D. Nguyen ◽  
Holden Verdeprado ◽  
Demeter Zita ◽  
Sachiyo Sanada-Morimura ◽  
Masaya Matsumura ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Genes ◽  
Genetic Background ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Japonica Rice ◽  
Rice Varieties ◽  
Nilaparvata Lugens Stål

The brown planthopper (BPH: Nilaparvata lugens Stål.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice’s resistance to BPH in the context of regionally increasing levels of virulence.

scholarly journals Substitution mapping and characterization of brown planthopper resistance genes from indica rice variety, ‘PTB33’ (Oryza sativa L.)

2021 ◽  
Author(s):  
Cuong Dinh Nguyen ◽  
Shao-Hui Zheng ◽  
Sachiyo Sanada-Morimura ◽  
Masaya Matsumura ◽  
Hideshi Yasui ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Genes ◽  
Rice Variety ◽  
Brown Planthopper ◽  
Substitution Mapping ◽  
Brown Planthopper Resistance

scholarly journals Respons Beberapa Varietas Padi (Oryza sativa L.) terhadap Wereng Batang Coklat (Nilaparvata lugens Stall) Koloni Karawang

Agrikultura ◽  
2018 ◽  
Vol 29 (2) ◽  
pp. 73
Author(s):  
Dedi Darmadi ◽  
Tuti Alawiyah
Keyword(s):  
Oryza Sativa ◽  
Rice Yield ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Test Method ◽  
Rice Varieties ◽  
Directorate General ◽  
Population Origin

ABSTRACTResponse of rice varieties (Oryza sativa L.) to brown planthopper (Nilaparvata lugens Stall) of Karawang coloniesThe research was conducted to determine the resistant level of several rice varieties (Oryza sativa L.) against brown planthopper (Nilaparvata lugens Stall) Karawang colony. The experiment was done at the Entomology Laboratory of Pest Forcesting Institute, Jatisari, Directorate General of Food Crops, Ministry of Agriculture. It was conducted from February to June 2017. The resistant of rice varieties against brown planthopper was tested using honey dew test method with bromocressol green as indicator. Honey dew test method is one of methods to evaluate rice variety reaction against brown planthopper population origin of certain area. This is due to the production of honey dew by brown planthopper which positively correlated with the amount of liquid being drawn from the plant. This study used Duncan Test on 5% level. The result demonstrated the variation of rice resistant against brown planthopper based on the area of honey dew spot, brown planthover mortality, attacks intensity, the destruction level on plant, and the rice yield. Inpari 13 was the most toleranct rice variety against the infestation of brown planthopper of Karawang colony.Keywords: Brown planthopper, Honey dew, Paddy, Resistance, VarietiesABSTRAKPenelitian ini bertujuan untuk menilai tingkat ketahanan varietas padi (Oryza sativa L.) terhadap wereng batang coklat (Nilaparvata lugens Stall) koloni Karawang. Penilitian dilaksanakan di Laboratorium Entomologi Balai Besar Peramalan Organisme Pengganggu Tumbuhan (BBPOPT) Jatisari, Direktorat Jenderal Tanaman Pangan, Kementerian Pertanian. Penelitian berlangsung dari Bulan Februari sampai Bulan Juni 2017. Pengujian ketahanan varietas padi terhadap wereng batang coklat dilakukan dengan menggunakan metode uji embun madu (honeydew test) dengan memakai indicator bromocressol green. Metode uji embun madu merupakan salah satu metode untuk mengevaluasi reaksi suatu varietas terhadap populasi wereng batang coklat yang berkembang di suatu wilayah tertentu, karena jumlah embun madu yang dikeluarkan oleh wereng batang coklat mempunyai hubungan yang positif dengan jumlah cairan tanaman yang dihisap. Uji lanjut yang digunakan adalah uji lanjut Duncan pada taraf 5%. Hasil penelitian menunjukkan terdapat variasi ketahanan antar varietas padi yang diuji dilihat dari luas bercak embun madu yang dihasilkan pada masing-masing varietas padi, mortalitas wereng batang coklat, intensitas serangan, tingkat kerusakan tanaman, dan bobot hasil panen padi. BErdasarkan parameter tersebut di atas, Inpari 33 merupakan varietas padi yang tahan terhadap serangan wereng batang coklat koloni Karawang.Kata kunci : Embun madu, Ketahanan, Padi, Varietas, Wereng batang coklat

scholarly journals Mapping of Resistance Genes to Brown Planthopper in Untup Rajab, an Indonesian Local Rice Variety

2018 ◽  
Vol 14 (2) ◽  
pp. 75
Author(s):  
Muhamad Yunus ◽  
Diani Damayanti ◽  
Ahmad Dadang ◽  
Ahmad Warsun ◽  
Dani Satyawan ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Quantitative Resistance ◽  
Insect Pest ◽  
Rice Variety ◽  
Brown Planthopper ◽  
Snp Markers ◽  
Chromosome 8 ◽  
F2 Population ◽  
Resistant Varieties ◽  
Test Population

<p>Brown planthopper (BPH) is a major rice pest in Indonesia. The most economical and effective approach to control the insect pest is by using resistant varieties. Exploring for resistance genes is, therefore, a prerequisite for effective breeding program for BPH resistance. This study aimed to map BPH resistance genes in Untup Rajab, an Indonesian local rice variety. Genetic map was constructed using an F2 population from a cross between TN-1 and Untup Rajab, and SNP markers from RiceLD SNP Chip. Phenotyping was performed using bulk seedling test on F2:3 seedlings against two BPH populations, i.e. X1 and S1. Four QTLs<br />were identified on chromosomes 5, 6, 8, and 11 with PVE values of 7.63%, 9.40%, 17.66%, and 3.05%, respectively. Relatively normal distribution of resistance phenotype and the relatively low PVE values indicate that Untup Rajab has a quantitative resistance to BPH with two different resistance loci identified for each BPH test population. The QTL on chromosome 8 overlaps with OsHI-LOX gene, which is associated with resistance to BPH, and adjacent to another QTL for resistance to green leafhopper. The QTL on chromosome 6 was found near OsPLDα4 and OsPLDα5 genes which are related to BPH resistance. Meanwhile, the QTL intervals on chromosome 5 and 11 did not overlap with any known BPH QTLs or genes, which make them attractive candidates for novel BPH resistance gene discovery.</p>

scholarly journals Recent Strategies for Detection and Improvement of Brown Planthopper Resistance Genes in Rice: A Review

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1202
Author(s):  
Bello Sani Haliru ◽  
Mohd Y. Rafii ◽  
Norida Mazlan ◽  
Shairul Izan Ramlee ◽  
Isma’ila Muhammad ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Large Scale ◽  
Insect Pest ◽  
Durable Resistance ◽  
Brown Planthopper ◽  
Gene Clusters ◽  
Nilaparvata Lugens ◽  
Scale Production ◽  
Rice Varieties ◽  
Resistant Genes

Brown planthopper (BPH; Nilaparvata lugens Stal) is considered the main rice insect pest in Asia. Several BPH-resistant varieties of rice have been bred previously and released for large-scale production in various rice-growing regions. However, the frequent surfacing of new BPH biotypes necessitates the evolution of new rice varieties that have a wide genetic base to overcome BPH attacks. Nowadays, with the introduction of molecular approaches in varietal development, it is possible to combine multiple genes from diverse sources into a single genetic background for durable resistance. At present, above 37 BPH-resistant genes/polygenes have been detected from wild species and indica varieties, which are situated on chromosomes 1, 3, 4, 6, 7, 8, 9, 10, 11 and 12. Five BPH gene clusters have been identified from chromosomes 3, 4, 6, and 12. In addition, eight BPH-resistant genes have been successfully cloned. It is hoped that many more resistance genes will be explored through screening of additional domesticated and undomesticated species in due course.

Relationships of brown planthopper resistance to tungro virus and grain characteristics in rice

1987 ◽  
Vol 109 (3) ◽  
pp. 609-610 ◽  
Author(s):  
G. S. V. Prasad ◽  
M. V. S. Sastry ◽  
J. R. K. Rao ◽  
A. Ghosh ◽  
Y. Kondala Rao
Keyword(s):  
Oryza Sativa ◽  
Insect Pests ◽  
Effective Means ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Cultivars ◽  
Genetics Of Resistance ◽  
Green Leafhopper ◽  
Resistant Varieties ◽  
Brown Planthopper Resistance

Brown planthopper, Nilaparvata lugens (Stal), is one of the most serious insect pests of rice (Oryza sativa L.) throughout Asia. Introduction of resistant varieties could be an effective means of minimizing losses from the pest. Several hundred rice cultivars resistant to the pest have been identified and the genetics of resistance has been analysed. Studies have shown that the genes for resistance to brown planthopper, bph-4, and to green leafhopper, Glh-3, are linked (Sidhu & Khush, 1979). Ideka & Kaneda (1983) reported that bph-2 for brown planthopper resistance was linked with the gene d2 for dwarfness. The present study sought to ascertain relationships between resistance to brown planthopper and tungro virus and grain characteristics.

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