scholarly journals Role of Cassava CC-type Glutaredoxin MeGRXC3 in Regulating Sensitivity to Mannitol-Induced Osmotic Stress Dependent on its Nuclear Activity

2021 ◽  
Author(s):  
Meng-Bin Ruan ◽  
Xiao-Ling Yu ◽  
Xin Guo ◽  
Ping-Juan Zhao ◽  
Ming Peng
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Nuclear Localization ◽  
Potential Role ◽  
Nuclear Activity ◽  
Transactivation Activity ◽  
Transcription Factor Genes ◽  
Related Transcription Factor ◽  
Stress Dependent

Abstract BackgroundWe previously identified six drought-inducible CC-type glutaredoxins in cassava cultivars, however, less is known about their potential role in the molecular mechanism by which cassava adapted to abiotic stress. ResultsHerein, we investigate one of cassava drought-responsive CC-type glutaredoxins, namely MeGRXC3, that involved in regulation of mannitol-induced inhibition on seed germination and seedling growth in transgenic Arabidopsis. MeGRXC3 overexpression up-regulates several stress-related transcription factor genes, such as PDF1.2, ERF6, WRKY40, and WRKY53 in Arabidopsis. Protein interaction assays show that MeGRXC3 interacts with Arabidopsis TGA2 and TGA5 in the nucleus. Eliminated nuclear localization of MeGRXC3 failed to result mannitol-induced inhibition of seed germination and seedling growth in transgenic Arabidopsis. Mutation analysis of MeGRXC3 indicates the importance of conserved motifs for its transactivation activity in yeast. Additionally, these motifs are also indispensable for its functionality in regulating mannitol-induced inhibition of seed germination and enhancement of the stress-related transcription factors in transgenic Arabidopsis.ConclusionsMeGRXC3 overexpression confers mannitol sensitivity in transgenic Arabidopsis possibly through interaction with TGA2/5 in the nucleus, and nuclear activity of MeGRXC3 is required for its function.

Role of HD2 genes in seed germination and early seedling growth in Arabidopsis

2011 ◽  
Vol 30 (10) ◽  
pp. 1969-1979 ◽  
Author(s):  
Adam Colville ◽  
Reem Alhattab ◽  
Ming Hu ◽  
Hélène Labbé ◽  
Tim Xing ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Early Seedling Growth ◽  
Early Seedling

scholarly journals The fate of the primary cilium during myofibroblast transition

2014 ◽  
Vol 25 (5) ◽  
pp. 643-657 ◽  
Author(s):  
Matthew Rozycki ◽  
Monika Lodyga ◽  
Jessica Lam ◽  
Maria Zena Miranda ◽  
Károly Fátyol ◽  
...  
Keyword(s):  
Growth Factor ◽  
Sonic Hedgehog ◽  
Primary Cilium ◽  
Potential Role ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Related Transcription Factor ◽  
Necessary And Sufficient ◽  
Organ Fibrosis

Myofibroblasts, the culprit of organ fibrosis, can originate from mesenchymal and epithelial precursors through fibroblast–myofibroblast and epithelial–myofibroblast transition (EMyT). Because certain ciliopathies are associated with fibrogenesis, we sought to explore the fate and potential role of the primary cilium during myofibroblast formation. Here we show that myofibroblast transition from either precursor results in the loss of the primary cilium. During EMyT, initial cilium growth is followed by complete deciliation. Both EMyT and cilium loss require two-hit conditions: disassembly/absence of intercellular contacts and transforming growth factor-β1 (TGFβ) exposure. Loss of E-cadherin–dependent junctions induces cilium elongation, whereas both stimuli are needed for deciliation. Accordingly, in a scratch-wounded epithelium, TGFβ provokes cilium loss exclusively along the wound edge. Increased contractility, a key myofibroblast feature, is necessary and sufficient for deciliation, since constitutively active RhoA, Rac1, or myosin triggers, and down-regulation of myosin or myocardin-related transcription factor prevents, this process. Sustained myosin phosphorylation and consequent deciliation are mediated by a Smad3-, Rac1-, and reactive oxygen species–dependent process. Transitioned myofibroblasts exhibit impaired responsiveness to platelet-derived growth factor-AA and sonic hedgehog, two cilium-associated stimuli. Although the cilium is lost during EMyT, its initial presence contributes to the transition. Thus myofibroblasts represent a unique cilium-less entity with profoundly reprogrammed cilium-related signaling.

scholarly journals Boron induces seed germination and seedling growth of Hordeum vulgare L. under Nacl stress

2018 ◽  
Vol 8 ◽  
pp. 1224-1234
Author(s):  
Saud A. Alamri ◽  
Manzer H Siddiqui ◽  
Mutahhar Y. Al-Khaishani ◽  
Hayssam M. Ali
Keyword(s):  
Hordeum Vulgare ◽  
Seed Germination ◽  
Seedling Growth ◽  
Growth Traits ◽  
Nacl Stress ◽  
High Dose ◽  
Barley Plant ◽  
Hordeum Vulgare L ◽  
Viable Cells

Boron (B), an essential micronutrient, helps the plants to complete their life cycle successfully. Therefore, the present experiment was conducted to study (1) the role of B in seed germination and seedling growth, (2) the toxicity effect of B in seed germination and seedling growth and (3) the role of B in tolerance of barley (Hordeum vulgare L. var. ‘Bakore’) to NaCl stress. Under NaCl stress and non-stress conditions, application of high levels of B (100 µM) decreased parameters of germination (G%, VI, GI and MGT), growth (RL, SL, RFW, SFW, RDW and SDW), except the accumulation of Pro and MDA in barley seedlings. Also, a fluorescence study reveals that production of ROS (H2O2 and O2 •—) and non-viable cells increased in roots of barley seedlings treated with NaCl and high dose of B. An alteration in anatomical structure of barley seedlings was observed with the application of NaCl and high dose of B. However, a low concentration of B (50 µM) proved best and increased all germination and growth traits of barley seedlings by increasing further accumulation of Pro. Also, 50 µM of B significantly increased the biosynthesis of photosynthetic pigments (Chl a, b and total Chl) and deceased formation of ROS and viable cells in roots. Therefore, concluded that sufficient dose of B could be beneficial for barley plant in improving the tolerance to NaCl stress.

Potential role of fungi and bacteria in Chinese fir replant soil

1994 ◽  
Vol 72 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Qishui Zhang ◽  
John C. Zak
Keyword(s):  
Seedling Growth ◽  
Root Rot ◽  
Soil Bacteria ◽  
Soil Fungi ◽  
Potential Role ◽  
Pathogenic Fungi ◽  
Chinese Fir ◽  
Biotic Factors ◽  
Replant Problem

The role of soil fungi and Bacillus in the Chinese fir (Cunninghamia lanceolata) replant problem was investigated. Several pathogenic fungi isolated were more abundant in replant than in non-replant woodland soil, but the species composition of soil bacteria showed no significant differences. Fumigation of replant soils with methyl bromide significantly increased the growth of Chinese fir compared with nonfumigated soils. Inoculation of steam-sterilized soil with the pathogenic fungi caused significant reduction of growth of Chinese fir seedlings, while amendment with bacteria from the soil had no effect on seedling growth. However, the combination of pathogenic fungi and bacteria greatly reduced plant growth and increased root rot compared with pathogenic fungi or bacteria alone. It is suggested that fungi alone or in combination with bacteria may be important biotic factors in the Chinese fir replant problem. Key words: fungi, bacteria, Chinese fir, replanted soil, seedling growth.

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