SiFBA5, a cold-responsive factor from Saussurea involucrate promotes cold resilience and biomass increase in transgenic tomato plants under cold stress

Abstract Background Saussurea involucrate survives in extreme arctic conditions with strong cold resistant ability. The species occurs in rocky, mountainous habitats at elevations of approximately 2400-4100m with year-round snowfall and freezing temperatures providing a magnificent model and abundant gene pool for dissecting plant stress responses. Fructose-1,6-Bisphosphate Aldolase (FBA) mediates the reverse conversion of fructose 1,6-bisphosphate (FBP) into dihydroxyacetone phosphate (DHAP) and glycerol triphosphate (GAP) during glycolysis or gluconeogenesis, which is the key enzyme in this reaction. Saussurea involucrate can survive in extremely low temperature environment, which shows that it has extremely high photosynthesis efficiency. Significantly, the underlying mechanism of its cold tolerance is yet to be unveiled. Therefore, our work aims to explore potential molecular mechanisms. Results In this study, we identified a cold-responsive gene SiFBA5 that based on a preliminary low-temperature genome-wide transcriptional profiling in S. involucrata. Expression analysis showed that cold temperature rapidly induced transcriptional expression of SiFBA5, suggesting that SiFBA5 participates in initial stress responses. Subcellular localization showed that SiFBA5 is localized to the chloroplast. We then generated transgenic tomato plants overexpressing SiFBA5 derived by a CaMV 35S promoter. Phenotypic observation suggested that the transgenic overexpress plants displayed increased cold tolerance, photosynthesis efficiency, and carbohydrate accumulation in comparison with wild-type plants. Conclusion Collectively, our results demonstrated that SiFBA5 positively regulates plant response to cold stress, making it a promising candidate for improving cold tolerance in crops.

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SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress

BMC Plant Biology ◽

10.1186/s12870-021-02851-8 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Jianqiang Mu ◽

Yajuan Fu ◽

Bucang Liu ◽

Yao Zhang ◽

Aiying Wang ◽

...

Keyword(s):

Stress Response ◽

Low Temperature ◽

Cold Stress ◽

Cold Tolerance ◽

Crop Yield ◽

Photosynthetic Efficiency ◽

Transgenic Tomato ◽

Saussurea Involucrata ◽

Tomato Plants ◽

Transgenic Tomato Plants

Abstract Background Saussurea involucrata survives in extreme arctic conditions and is very cold-resistant. This species grows in rocky, mountainous areas with elevations of 2400–4100 m, which are snow-covered year-round and are subject to freezing temperatures. S. involucrata’s ability to survive in an extreme low-temperature environment suggests that it has particularly high photosynthetic efficiency, providing a magnificent model, and rich gene pool, for the analysis of plant cold stress response. Fructose-1, 6-bisphosphate aldolase (FBA) is a key enzyme in the photosynthesis process and also mediates the conversion of fructose 1, 6-bisphosphate (FBP) into dihydroxyacetone phosphate (DHAP) and glycerol triphosphate (GAP) during glycolysis and gluconeogenesis. The molecular mechanisms underlying S. involucrata’s cold tolerance are still unclear; therefore, our work aims to investigate the role of FBA in plant cold-stress response. Results In this study, we identified a cold-responsive gene, SiFBA5, based on a preliminary low-temperature, genome-wide transcriptional profiling of S. involucrata. Expression analysis indicated that cold temperatures rapidly induced transcriptional expression of SiFBA5, suggesting that SiFBA5 participates in the initial stress response. Subcellular localization analysis revealed that SiFBA5 is localized to the chloroplast. Transgenic tomato plants that overexpressed SiFBA5 were generated using a CaMV 35S promoter. Phenotypic observation suggested that the transgenic plants displayed increased cold tolerance and photosynthetic efficiency in comparison with wild-type plants. Conclusion Cold stress has a detrimental impact on crop yield. Our results demonstrated that SiFBA5 positively regulates plant response to cold stress, which is of great significance for increasing crop yield under cold stress conditions.

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Identification of cold stress responsive microRNAs in cold tolerant and susceptible Hemerocallis fulva by high throughput sequencing

10.21203/rs.3.rs-41470/v1 ◽

2020 ◽

Author(s):

Changbing Huang ◽

Chun Jiang ◽

limin Jin ◽

Huanchao Zhang

Keyword(s):

Low Temperature ◽

Cold Stress ◽

Cold Tolerance ◽

Temperature Stress ◽

Stress Responses ◽

Target Genes ◽

Low Temperature Stress ◽

Differentially Expressed ◽

Differentially Expressed Mirnas ◽

Cold Tolerant

Abstract Background:Hemerocallis fulva is a perennial herb belonging to Hemerocallis of Hemerocallis. Because of the large and bright colors, it is often used as a garden ornamental plant. But most varieties of H. fulva on the market will wither in winter, which will affect their beauty. It is very important to study the effect of low temperature stress on the physiological indexes of H. fulva and understand the cold tolerance of different H. fulva. MiRNA is a kind of endogenous non coding small molecular RNA with length of 21-24nt. It mainly inhibits protein translation by cutting target genes, and plays an important role in the development of organisms, gene expression and biological stress. Low temperature is the main abiotic stress affecting the production of H. fulva in China, which hinders the growth and development of plants. A comprehensive understanding of the expression pattern of microRNA in H. fulva under low temperature stress can improve our understanding of microRNA mediated stress response. Although there are many studies on miRNAs of various plants under cold stress at home and abroad, there are few studies on miRNAs related to cold stress of H. fulva. It is of great significance to explore the cold stress resistant gene resources of H. fulva, especially the identification and functional research of miRNA closely related to cold stress, for the breeding of excellent H. fulva.Results A total of 5619 cold-responsive miRNAs, 315 putative novel and 5 304 conserved miRNAs, were identified from the leaves and roots of two different varieties ‘Jinyan’ (cold-tolerant) and ‘Lucretius ’ (cold-sensitive), which were stressed under -4 oC for 24 h. Twelve conserved and three novel miRNAs (novel-miR10, novel-miR19 and novel-miR48) were differentially expressed in leaves of ‘Jinyan’ under cold stress. Novel-miR19, novel-miR29 and novel-miR30 were up-regulated in roots of ‘Jinyan’ under cold stress. Thirteen and two conserved miRNAs were deferentially expressed in leaves and roots of ‘Lucretius’ after cold stress. The deferentially expressed miRNAs between two cultivars under cold stress include novel miRNAs and the members of the miR156, miR166 and miR319 families. A total of 6 598 target genes for 6 516 known miRNAs and 82 novel miRNAs were predicted by bioinformatic analysis, mainly involved in metabolic processes and stress responses. Ten differentially expressed miRNAs and predicted target genes were confirmed by quantitative reverse transcription PCR(q-PCR), and the expressional changes of target genes were negatively correlated to differentially expressed miRNAs. Our data indicated that some candidate miRNAs (e.g., miR156a-3-p, miR319a, and novel-miR19) may play important roles in plant response to cold stress.Conclusions Our study indicates that some putative target genes and miRNA mediated metabolic processes and stress responses are significant to cold tolerance in H. fulva.

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Enhancement of low-temperature tolerance in transgenic tomato plants overexpressing Lefad7 through regulation of trienoic fatty acids

Photosynthetica ◽

10.1007/s11099-013-0014-5 ◽

2013 ◽

Vol 51 (2) ◽

pp. 238-244 ◽

Cited By ~ 10

Author(s):

X. Y. Liu ◽

Y. B. Teng ◽

B. Li ◽

Q. W. Meng

Keyword(s):

Fatty Acids ◽

Low Temperature ◽

Temperature Tolerance ◽

Transgenic Tomato ◽

Tomato Plants ◽

Low Temperature Tolerance ◽

Transgenic Tomato Plants

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Dark respiration rate of transgenic tomato plants expressing FeSOD1 gene under chloride and sulfate salinity

Russian Agricultural Sciences ◽

10.3103/s1068367414010029 ◽

2014 ◽

Vol 40 (1) ◽

pp. 14-17 ◽

Cited By ~ 5

Author(s):

Ye. N. Baranova ◽

E. N. Akanov ◽

A. A. Gulevich ◽

L. V. Kurenina ◽

S. A. Danilova ◽

...

Keyword(s):

Respiration Rate ◽

Dark Respiration ◽

Transgenic Tomato ◽

Dark Respiration Rate ◽

Tomato Plants ◽

Transgenic Tomato Plants

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Genetic transactivation of Dissociation elements in transgenic tomato plants

MGG Molecular & General Genetics ◽

10.1007/bf00330561 ◽

1989 ◽

Vol 218 (1) ◽

pp. 25-32 ◽

Cited By ~ 31

Author(s):

Michael W. Lassner ◽

Joseph M. Palys ◽

John I. Yoder

Keyword(s):

Transgenic Tomato ◽

Tomato Plants ◽

Transgenic Tomato Plants

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Genome-Wide Analysis of the NAC Transcription Factor Gene Family Reveals Differential Expression Patterns and Cold-Stress Responses in the Woody Plant Prunus mume

Genes ◽

10.3390/genes9100494 ◽

2018 ◽

Vol 9 (10) ◽

pp. 494 ◽

Cited By ~ 17

Author(s):

Xiaokang Zhuo ◽

Tangchun Zheng ◽

Zhiyong Zhang ◽

Yichi Zhang ◽

Liangbao Jiang ◽

...

Keyword(s):

Cold Stress ◽

Gene Family ◽

Cold Tolerance ◽

Stress Responses ◽

Expression Profiles ◽

Expression Patterns ◽

Freezing Stress ◽

Prunus Mume ◽

Flower Bud ◽

Freezing Resistance

NAC transcription factors (TFs) participate in multiple biological processes, including biotic and abiotic stress responses, signal transduction and development. Cold stress can adversely impact plant growth and development, thereby limiting agricultural productivity. Prunus mume, an excellent horticultural crop, is widely cultivated in Asian countries. Its flower can tolerate freezing-stress in the early spring. To investigate the putative NAC genes responsible for cold-stress, we identified and analyzed 113 high-confidence PmNAC genes and characterized them by bioinformatics tools and expression profiles. These PmNACs were clustered into 14 sub-families and distributed on eight chromosomes and scaffolds, with the highest number located on chromosome 3. Duplicated events resulted in a large gene family; 15 and 8 pairs of PmNACs were the result of tandem and segmental duplicates, respectively. Moreover, three membrane-bound proteins (PmNAC59/66/73) and three miRNA-targeted genes (PmNAC40/41/83) were identified. Most PmNAC genes presented tissue-specific and time-specific expression patterns. Sixteen PmNACs (PmNAC11/19/20/23/41/48/58/74/75/76/78/79/85/86/103/111) exhibited down-regulation during flower bud opening and are, therefore, putative candidates for dormancy and cold-tolerance. Seventeen genes (PmNAC11/12/17/21/29/42/30/48/59/66/73/75/85/86/93/99/111) were highly expressed in stem during winter and are putative candidates for freezing resistance. The cold-stress response pattern of 15 putative PmNACs was observed under 4 °C at different treatment times. The expression of 10 genes (PmNAC11/20/23/40/42/48/57/60/66/86) was upregulated, while 5 genes (PmNAC59/61/82/85/107) were significantly inhibited. The putative candidates, thus identified, have the potential for breeding the cold-tolerant horticultural plants. This study increases our understanding of functions of the NAC gene family in cold tolerance, thereby potentially intensifying the molecular breeding programs of woody plants.

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Overexpression of LeNHX2 and SlSOS2 increases salt tolerance and fruit production in double transgenic tomato plants

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2018.11.028 ◽

2019 ◽

Vol 135 ◽

pp. 77-86 ◽

Cited By ~ 2

Author(s):

Mourad Baghour ◽

Francisco Javier Gálvez ◽

M. Elena Sánchez ◽

M. Nieves Aranda ◽

Kees Venema ◽

...

Keyword(s):

Salt Tolerance ◽

Fruit Production ◽

Transgenic Tomato ◽

Tomato Plants ◽

Transgenic Tomato Plants

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Control of Ethylene Synthesis by Expression of a Bacterial Enzyme in Transgenic Tomato Plants

The Plant Cell ◽

10.2307/3869226 ◽

1991 ◽

Vol 3 (11) ◽

pp. 1187 ◽

Cited By ~ 3

Author(s):

Harry J. Klee ◽

Maria B. Hayford ◽

Keith A. Kretzmer ◽

Gerard F. Barry ◽

Ganesh M. Kishore

Keyword(s):

Transgenic Tomato ◽

Ethylene Synthesis ◽

Tomato Plants ◽

Bacterial Enzyme ◽

Transgenic Tomato Plants

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Jasmonate and Melatonin Act Synergistically to Potentiate Cold Tolerance in Tomato Plants

Frontiers in Plant Science ◽

10.3389/fpls.2021.763284 ◽

2022 ◽

Vol 12 ◽

Author(s):

Fei Ding ◽

Liming Ren ◽

Fang Xie ◽

Meiling Wang ◽

Shuoxin Zhang

Keyword(s):

Cold Stress ◽

Cold Tolerance ◽

Feedback Loop ◽

Pcr Analysis ◽

Tomato Plants ◽

Qrt Pcr ◽

Cold Condition ◽

Tomato Cultivars ◽

Cold Responses ◽

Ja Signaling

Both jasmonic acid (JA) and melatonin (MT) have been demonstrated to play positive roles in cold tolerance, however, whether and how they crosstalk in the cold responses in plants remain elusive. Here, we report that JA and MT act synergistically in the cold tolerance in tomato plants (Solanum lycopersicum). It was found that JA and MT were both substantially accumulated in response to cold stress and foliar applications of methyl jasmonate (MeJA) and MT promoted cold tolerance as evidenced by increased Fv/Fm, decreased relative electrolyte leakage (EL) and declined H2O2 accumulation in tomato plants. Inhibition of MT biosynthesis attenuated MeJA-induced cold tolerance, while inhibition of JA biosynthesis reduced MT accumulation in tomato plants under cold conditions. Furthermore, qRT-PCR analysis showed that the expressions of two MT biosynthetic genes, SlSNAT and SlAMST, were strongly induced by MeJA, whereas suppression of SlMYC2, a master JA signaling regulator, abated the expressions of SlSNAT and SlAMST under cold stress. Additionally, suppression of SlMYC2 reduced MT accumulation, decreased Fv/Fm and increased EL in cold-stressed tomato plants. Interestingly, exogenous MT promoted JA accumulation, while inhibition of MT biosynthesis significantly reduced JA accumulation in tomato plants under the cold condition. Taken together, these results suggest that JA and MT act cooperatively in cold tolerance and form a positive feedback loop, amplifying the cold responses of tomato plants. Our findings might be translated into the development of cold-resistant tomato cultivars by genetically manipulating JA and MT pathways.

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