scholarly journals Mono(2-ethylhexyl) Phthalate Modulates the Lipid Accumulation and Reproductive Signaling in Daphnia Magna

2021 ◽  
Author(s):  
Hyunki Cho ◽  
Yohan Seol ◽  
Seungyun Baik ◽  
Baeckkyoung Sung ◽  
Chang Seon Ryu ◽  
...  
Keyword(s):  
Daphnia Magna ◽  
Exposure Time ◽  
Lipid Accumulation ◽  
Aquatic Organisms ◽  
Treated Group ◽  
Ecdysone Receptor ◽  
Female Adult ◽  
Primary Metabolite ◽  
Lipid Metabolites ◽  
Ethylhexyl Phthalate

Abstract Mono(2-ethylhexyl)-phthalate (MEHP) is the primary metabolite of di(2-ethylhexyl)-phthalate (DEHP), which is widely used in industry as a plasticizer. Previous studies showed that both DEHP and MEHP have been found in not only human urine samples but also natural aquatic environments, and well documented as toxicants for reproduction and endocrine disruptors, However, the effects of MEHP exposure on aquatic organisms, including invertebrates such as Daphnia magna (D. magna), are still scarce. In the present study, the lipid alterations caused by MEHP in D. magna have been identified by analyzing lipid accumulation and nontarget metabolomics. Additionally, the expressions of reproduction were investigated. Ecotoxicologically relevant concentrations (1 to 2 mg/L) and exposure time ranges (24 h to 21 days) have been tested. MEHP resulted in no mortality to D. magna with all exposure conditions, but the number of lipid droplets increased after 96 h of exposure and enhanced reproduction of female adult daphnids was observed in the 21-day of exposure. Besides, we observed that MEHP enhanced lipid metabolism with the identification of 283 potential lipid metabolites, including glycerolipids, glycerophospholipids, and sphingolipids, following 48 h of exposure. MEHP treated group exhibited significantly higher expression of ecdysone receptor (EcR) and vitellogenin 2 (Vtg2) at 6 h and 24 h. Meanwhile at 48 h, EcR and Vtg2 were downregulated in 1 and 2 mg/L MEHP exposure. Our data show that the changes in EcR pathway with different exposure time could be associated with the lipid accumulation due to lipids increment subsequently to increased reproduction in MEHP-exposed D. magna.

Interactive toxic effects of agrochemicals on aquatic organisms

1999 ◽  
Vol 40 (1) ◽  
pp. 357-364 ◽  
Author(s):  
A. Kungolos ◽  
P. Samaras ◽  
A. M. Kipopoulou ◽  
A. Zoumboulis ◽  
G. P. Sakellaropoulos
Keyword(s):  
Daphnia Magna ◽  
Marine Bacterium ◽  
Methyl Parathion ◽  
Vibrio Fischeri ◽  
Interactive Effect ◽  
Algal Growth ◽  
Aquatic Organisms ◽  
Interactive Effects ◽  
Selenastrum Capricornutum ◽  
Toxic Compound

The effects of three common agrochemicals, lindane, methyl parathion and atrazine, on crustacean Daphnia magna, alga Selenastrum capricornutum and marine bacterium Vibrio fischeri were investigated in this study. Methyl parathion was the most toxic compound towards all three organisms, while lindane was more toxic to Daphnia magna and Vibrio fischeri than atrazine, and atrazine was more toxic to Selenastrum capricornutum than lindane. Among the three aquatic organisms, Selenastrum capricornutum was most sensitive in detecting lindane and atrazine toxicity, while Daphnia magna was most sensitive in detecting methyl parathion toxicity. The interactive effects of the pesticides were also investigated. The interactive effect between lindane and methyl parathion on survival of Daphnia magna was synergistic, while the ones between lindane and atrazine and between methyl parathion and atrazine were generally additive. The interactive effect of the three pesticides applied together on Daphnia magna was synergistic. The interactive effect of the three pesticides on the growth of Selenastrum capricornutum was antagonistic with few cases of addition, while the effect of all the three pairs of pesticides on algal growth was also antagonistic. The interactive effect of lindane and methyl parathion on Vibrio fischeri was additive.

scholarly journals Development of Daphnia magna under exposure to the xenobiotic octylphenol

2014 ◽  
Vol 6 (2) ◽  
pp. 155-158
Author(s):  
Thanh Son Dao ◽  
Thi My Chi Vo ◽  
Hong Lan Chi Do ◽  
Phuoc Dan Nguyen
Keyword(s):  
Developing Country ◽  
Daphnia Magna ◽  
Aquatic Organisms ◽  
Environmental Concerns ◽  
Human Beings ◽  
Ecological Health ◽  
Potential Toxicity ◽  
Chronic Effects ◽  
High Concentrations ◽  
Significant Mortality

Xenobiotics are of human and environmental concerns due to their potential toxicity. Octylphenol is one of the very common and daily used xenobiotics in door and out door activities of human beings. Toxicity of octylphenol to aquatic organisms, especially to zooplankton (e.g. Daphnia magna) was investigated but not fully understood. In this study we evaluated the chronic effects of octylphenol at the concentrations of 5, 50 and 500 µg L-1 on Daphnia magna over a period of 14 days. The results showed that low concentration of octylphenol (5 µg L-1) stimulated the maturation while high concentrations of the chemical (50 and 500 µg L-1) caused a significant mortality to the Daphnia. Besides, all the tested concentrations of octylphenol had serious impacts on fecundity and growth of the animals. Investigations on the presence, distribution, fate and toxicity of xonobiotics including octylphenol in the developing country environment are suggested for human, environmental and ecological health protection. Những hợp chất tổng hợp đang là mối quan ngại cho con người và môi trường vì khả năng gây độc của chúng. Octylphenol là một trong những hợp chất tổng hợp được sử dụng phổ biến và thường xuyên trong những hoạt động của con người trong nhà và ngoài trời. Độc tính của octylphenol đối với thủy sinh vật, đặc biệt đối với động vật phù du (vd. Daphnia magna) mặc dù đã được nghiên cứu nhưng vẫn chưa được hiểu biết đầy đủ. Trong nghiên cứu này, chúng tôi đánh giá ảnh hưởng mãn tính của octylphenol ở các nồng độ 5, 50 và 500 µg/lít lên Daphnia magna trong thời gian 14 ngày. Kết quả cho thấy ở nồng độ octylphenol thấp (5 µg/lít) kích thích sự thành thục của sinh vật trong khi ở nồng độ cao hơn (50 và 500 µg/lít) gây chết đáng kể Daphnia. Bên cạnh đó, tất cả các nồng độ ocytlphenol dùng trong thí nghiệm gây ảnh hưởng nghiêm trọng lên sức sinh sản và sinh trưởng của sinh vật. Nghiên cứu về sự hiện diện, phân bố, phát tán và độc tính của những chất tổng hợp bao gồm octylphenol ở các nước đang phát triển nên được tiến hành vì mục tiêu bảo vệ sức khỏe con người, môi trường và hệ sinh thái.

Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells

2019 ◽  
Vol 184 ◽  
pp. 109611 ◽  
Author(s):  
Yuezhu Zhang ◽  
Shuyue Wang ◽  
Tianyang Zhao ◽  
Liwei Yang ◽  
Shuangyu Guo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Accumulation ◽  
Ethylhexyl Phthalate

scholarly journals Bioaccumulation Potential of CPs in Aquatic Organisms: Uptake and Depuration in Daphnia magna

2019 ◽  
Vol 53 (16) ◽  
pp. 9533-9541 ◽  
Author(s):  
Mafalda Castro ◽  
Anna Sobek ◽  
Bo Yuan ◽  
Magnus Breitholtz
Keyword(s):  
Daphnia Magna ◽  
Aquatic Organisms

Effect of the TYK-2/STAT-3 pathway on lipid accumulation induced by mono-2-ethylhexyl phthalate

2019 ◽  
Vol 484 ◽  
pp. 52-58 ◽  
Author(s):  
Wen Qi ◽  
Liting Zhou ◽  
Tianye Zhao ◽  
Shuang Ding ◽  
Qi Xu ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Ethylhexyl Phthalate

scholarly journals Embryonic exposures to mono-2-ethylhexyl phthalate induce larval steatosis in zebrafish independent of Nrf2a signaling

2020 ◽  
pp. 1-9
Author(s):  
Karilyn E. Sant ◽  
Hadley M. Moreau ◽  
Larissa M. Williams ◽  
Haydee M. Jacobs ◽  
Anna M. Bowsher ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Steatosis ◽  
Antioxidant Response ◽  
Abnormal Development ◽  
Fish Length ◽  
Peroxisome Proliferator ◽  
Primary Metabolite ◽  
Peroxisome Proliferator Activated Receptor ◽  
Expression Of Genes ◽  
Ethylhexyl Phthalate

Abstract Mono-2-ethylhexyl phthalate (MEHP) is the primary metabolite of the ubiquitous plasticizer and toxicant, di-2-ethylhexyl phthalate. MEHP exposure has been linked to abnormal development, increased oxidative stress, and metabolic syndrome in vertebrates. Nuclear factor, Erythroid 2 Like 2 (Nrf2), is a transcription factor that regulates gene expression in response to oxidative stress. We investigated the role of Nrf2a in larval steatosis following embryonic exposure to MEHP. Wild-type and nrf2a mutant (m) zebrafish embryos were exposed to 0 or 200 μg/l MEHP from 6 to either 96 (histology) or 120 hours post fertilization (hpf). At 120 hpf, exposures were ceased and fish were maintained in clean conditions until 15 days post fertilization (dpf). At 15 dpf, fish lengths and lipid content were examined, and the expression of genes involved in the antioxidant response and lipid processing was quantified. At 96 hpf, a subset of animals treated with MEHP had vacuolization in the liver. At 15 dpf, deficient Nrf2a signaling attenuated fish length by 7.7%. MEHP exposure increased hepatic steatosis and increased expression of peroxisome proliferator-activated receptor alpha target fabp1a1. Cumulatively, these data indicate that developmental exposure alone to MEHP may increase risk for hepatic steatosis and that Nrf2a does not play a major role in this phenotype.

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