Analysis of Internal Transcribed Spacer Regions II Gene and Morphology of Paragonimus From Yunnan Province, China

Author(s):  
Binbin Yang ◽  
Juan Li ◽  
Benjiang Zhou

Abstract For a long time, there is no clear-cut to identify some species of paragonimus in Yunnan Province, China. This paper involved the distribution of Paragonimus in Jinping country and Baoshan city, Yunnan province. In this experiment, the metacercariae, excysted metacercariae, eggs, adult worms were obtained from different hosts were observed and measured. Cats have been described as the appropriate definitive host of paragonimus sp., which are closed to P. cheni according to morphology. Especially the ovaries of adult worms are few and has no third branches. With SEM observation, their spines in the surface are single, a sharp-pointed knife or half-moon in shape and the end of a few of spines are bifurcate. While the clustered sequences strains of this study in the ITS2 tree clustered of Yunan is out sider of P. skirjabini complex and have the genetically greater distances than other isolates of the P. skrjabini complex. Therefore, the Paragonimus sp. of this study from Jinping County and Baoshan city are the same subspecies of P. skrjabini complex.

2007 ◽  
Vol 45 (3) ◽  
pp. 1079-1079
Author(s):  
S. N. Leaw ◽  
H. C. Chang ◽  
H. F. Sun ◽  
R. Barton ◽  
J.-P. Bouchara ◽  
...  

Phytotaxa ◽  
2021 ◽  
Vol 482 (3) ◽  
pp. 251-260
Author(s):  
TIAN-JUN YUAN ◽  
OLIVIER RASPÉ ◽  
YONG-JUN LI ◽  
LI WANG ◽  
KAI-MEI SU ◽  
...  

A new hypogeous species, Choiromyces cerebriformis sp. nov. is described and illustrated from Yunnan province, China. Both morphological evidence and sequence analyses of the internal transcribed spacer region (ITS1-5.8S-ITS2) support the species as new to science. C. cerebriformis differs from other Choiromyces species in having ascomata with larger lobes and light orange-brown gleba, and globose ascospores with short stick-like sparse spines ornamentation. Morphological differences and genetic distances with the similar species C. helanshanensis and C. alveolatus are discussed. A phenotypic key including related species is provided.


Phytotaxa ◽  
2018 ◽  
Vol 348 (3) ◽  
pp. 187 ◽  
Author(s):  
SAJEEWA S.N. MAHARACHCHIKUMBURA ◽  
ZONG-LONG LUO ◽  
HONG-YAN SU ◽  
ABDULLAH M. AL-SADI ◽  
RATCHADAWAN CHEEWANGKOON

Asexual members of Reticulascaceae (Glomerellales, Hypocreomycetidae) are generally hyphomycetes and usually found as saprobes occurring on various hosts in lentic habitats. A brief survey of the freshwater fungi in Yunnan Province, China, was carried out and four species of Reticulascaceae were isolated. Morphological examination and sequence analysis of large subunit of the nuclear ribosomal RNA (LSU), RNA polymerase II gene encoding the second largest protein subunit (RPB2) and internal transcribed spacer (ITS) regions provide evidence for two taxonomic novelties of Kylindria (K. aquatica and K. chinensis). Two other collections comprised two previously described species of Cylindrotrichum (C. clavatum and C. gorii). Descriptions and illustrations of the above taxa are provided and new species are discussed with comparable taxa.


2006 ◽  
Vol 52 (Special Issue) ◽  
pp. S28-S34 ◽  
Author(s):  
V.V. Podrázský

Results obtained during 14 years of the experiment are presented. Extreme site conditions are determined by: 8<sup>th</sup> vegetation altitudinal zone, podzolic soil and hard climate – average annual temperature 4°C, average annual precipitation around 1,200 mm. Effects of surface experimental liming were studied on immission clear-cuts in long time period, study area is located on the Velká Deštná locality on the main Orlické hory Mts. range. Research plots were established and the basic survey was done in spring of 1988, studies of liming effects were performed in the period 1987–2002. On particular plots, 0, 1,308, 2,826, 3,924 and 8,478 kg/ha of fine limestone (grain size under 1 mm) was distributed by hands on the soil surface, as an application of 3 and 9 t/ha of “fine” and “coarse” material (together 5 variants including the control one). Results are available for the forestry practice, research has to continue in the future – potential of negative liming impacts is topical here, consisting in humus mineralization and nutrients losses. Maximum effects on the soil reaction were observed in 8–10 years after limestone application at the soil surface (F – horizon) and in the period 10–15 years lower (H, A – horizons). Base saturation dynamics shows the time delay 2–4 years following pH values. For the site protection, efficient forest stands are vitally important of pioneer as well as climax species.


2011 ◽  
Vol 62 (2) ◽  
pp. 797-803 ◽  
Author(s):  
Shafiquzzaman Siddiquee ◽  
Weng Yan Yee ◽  
Khanam Taslima ◽  
Nur Hasan Nudin Fatihah ◽  
S. Vijay Kumar ◽  
...  

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