Construction of A High-Density SNP Genetic Map by Genotyping-By-Sequencing and Location of Seed Quality traits in Flax (Linum usitatisimum L.)

Abstract Background Oil and five main fatty acid content(especially linolenic acid content (LNA) )are six main quality traits in flax, and also the important target traits in flax breeding. However, the reports on genetic map construction and QTL mapping of linolenic acid content in flax are limited, which results in low accuracy and efficiency of quality breeding in oil flax. Construction the genetic linkage map and location the QTLs to discover the stable genetic QTLs sites related to oil content and fatty acid content, so as to provide targeted targets for breeders and improve breeding efficiency. Results In this study, a final integrated map consisting of 2,239 single nucleotide polymorphism (SNP) markers on 15 linkage groups (LGs) with an average distance of 0.46 cM between adjacent markers was generated using genotyping-by-sequencing (GBS) technique. A total of 21 quantitative trait loci (QTL)s for the six traits, i.e., palmitic acid content (PAL), stearic acid content (STE), oleic acid content (OLE), linoleic acid content (LIN), linolenic acid content (LNA), and oil content (OIL) in the RIL population under three environments. One QTL cluster harbored two QTLs for LIN and LNA trait, respectively was also identified. Especially, QTL qLIN-Group12-2 for LIN and QTL qLNA-Group12-2 for LNA on chromosome 12 were detected in multi-environments. Conclusions A high-density SNP genetic map with total 2239 markers was constructed with GBS technique, The total genetic distance of the SNP map was 1032.90 cM, with the average genetic distance of 0.46 cM per marker. A total of 21 quantitative trait loci (QTL)s for the six traits under three environments were determined. One QTL cluster harbored two QTLs for LIN and LNA trait, respectively was also identified. QTL qLIN-Group12-2 for LIN and QTL qLNA-Group12-2 for LNA on chromosome 12 were detected in multi-environments. These results provide more information for determining the seed quality related candidate genes and contribute to the marker-assisted selection in flax breeding.

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Effect of transplanting date on the fatty acid composition, oil content and yield of evening primrose (Oenothera biennis L.) seed

Canadian Journal of Plant Science ◽

10.4141/cjps94-023 ◽

1994 ◽

Vol 74 (1) ◽

pp. 129-131 ◽

Cited By ~ 1

Author(s):

Robert C. Roy ◽

Peter H. White ◽

Alex F. More ◽

John G. Hendel ◽

Robert Pocs ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Key Words ◽

Linolenic Acid ◽

Acid Content ◽

Oil Content ◽

Linolenic Acid Content ◽

Oenothera Biennis ◽

Evening Primrose ◽

One Year

The influence of transplanting time on the growth of evening primrose (Oenothera biennis L.) was investigated for 3 yr. Delaying transplanting reduced the yield of seed in two of these years. The amount of oil in the seed was not influenced by time of transplanting, and only in one year did the γ-linolenic acid content of the oil decrease with delays in transplanting. Key words: Evening primrose, transplanting date, oil content, γ-linolenic acid

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Goat yoghurt drinks with elevated α-linolenic acid content and enriched with yacon fiber

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1031 ◽

2019 ◽

Vol 13 (1) ◽

pp. 150-156

Author(s):

Markéta Borková ◽

Miloslav Šulc ◽

Alena Svitáková ◽

Klára Novotná ◽

Jana Smolová ◽

...

Keyword(s):

Fatty Acid ◽

Linolenic Acid ◽

Fatty Acid Profile ◽

Acid Content ◽

Linseed Oil ◽

Fatty Acid Content ◽

Goat Milk ◽

Linolenic Acid Content ◽

Omega 3 ◽

Feed Composition

Goat milk and goat milk products are very valuable in human nutrition because of their favorable nutrient composition which can be further boosted by the addition of prebiotic fiber and probiotic bacteria. It has also been possible to change the fatty acid profile of goat milk through feed composition. The aim of this study was to increase the nutritional value of goat milk by producing a probiotic yoghurt drink made from milk with elevated omega-3 fatty acids and enriched with natural yacon prebiotics. Goat nutrition is one of the key factors how we can naturally increase omega-3 fatty acid content in goat milk. In our study, twenty four White Shorthair goats were divided into the control and experimental group which was supplemented with 55 mL of linseed oil per day for eight weeks to increase the monounsaturated and polyunsaturated fatty acid content in the milk. The yoghurt milk drinks were formulated from individual goat milk samples with added bifidobacteria and yacon prebiotics. Our results showed that goat feed supplementation with linseed oil indeed positively changed fatty acid profile of goat milk in which α-linolenic acid content increased while, at the same time, lauric, myristic and palmitic acid contents decreased. Also, yoghurt drinks enriched with yacon prebiotics have shown higher bifidobacteria counts compared to the control.

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Effets physiologiques de la benzyladénine sur Lemna minor I. Influence sur la composition lipidique

Canadian Journal of Botany ◽

10.1139/b82-099 ◽

1982 ◽

Vol 60 (6) ◽

pp. 758-764 ◽

Cited By ~ 4

Author(s):

R. Bérubé ◽

G. Beaumont ◽

G. Grenier

Keyword(s):

Fatty Acid ◽

Linoleic Acid ◽

Linolenic Acid ◽

Acid Content ◽

Neutral Lipids ◽

Lemna Minor ◽

Fatty Acid Content ◽

Linolenic Acid Content ◽

High Doses ◽

Higher Doses

Doses from 0.002 to 2.0 ppm of benzyladenine (BA) increased the water content in 15-day-old plants of Lemna minor L. However, a concentration of 5.0 ppm decreased it. The total esterified fatty acid content increased up to 2.0 ppm and decreased at 5.0 ppm, compared with controls. The BA increased the percentages of total palmitic and α-linolenic acids and decreased the percentage of total linoleic acid, mainly for higher doses. At 2.0 and 5.0 ppm of BA, the proportions of phospholipids increased strongly as compared with galactolipids (especially the diacylgalactosylglycerol) and total neutral lipids. In phosphatidylcholine, phosphatidylethanolamine, and phosphotidylinositol, the α-linolenic acid content exhibited a marked increase at the expense of linoleic acid of these phospholipids. The percentage of α-linolenic acid in diacylgalactosylglycerol remained constant in the presence of BA, but it decreased in diacyldigalactosylglycerol. At concentrations of 2.0 and 5.0 ppm, the α-linolenic acid content of total neutral lipids increased greatly. The modifications observed in the lipid composition of L. minor, at 2.0 and 5.0 ppm in BA, suggest that the cell membranes (particularly those of chloroplasts) and their functions may be altered by these high doses of cytokinin.

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