Antineoplastic Efectiveness of Silver Nanoparticles Synthesized from Onopordum Acanthium L. extract (AgNPs-OAL) toward MDA-MB231 Breast Cancer Cells

2021 ◽  
Author(s):  
Romina Delalat ◽  
Seyed Ataollah Sadat Shandiz ◽  
Bahareh Pakpour
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Annexin V ◽  
Spherical Shape ◽  
Hek293 Cells ◽  
Apoptosis Pathway ◽  
Onopordum Acanthium

Abstract The present research was done to investigate the anticancer properties of silver nanoparticles (AgNPs) fabricated using bioactive extract of Onopordum acanthium L. (AgNPs-OAL) against breast cancer cell MDA_MB231 in vitro. The determination studies of AgNPs-OAL were confirmed by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) analysis. Interestingly, FESEM image observed the spherical shape of AgNPs-OAL with the range of 1–100 nm. As AgNP-OAL exhibited significant cytotoxicity properties on breast cancer MDA_MB231 cells with IC50 values of 66.04 μg/mL, while lowing toxicity toward normal human embryonic kidney 293 (HEK293) cells with IC50 values of 101.04 μg/mL was evaluated. Further, up-regulation of apoptotic Bax and CAD genes expressions were confirmed by quantitative real-time reverse transcription-PCR (qRT-PCR) technique results. Moreover, enhanced cell cycle population (sub-G1), annexin V/PI staining, acridine orange and ethidium bromide (AO/EB) staining, Hoescht 33258 dye, and generation of reactive oxygen species (ROS) observed in AgNP-OAL-treated MDA_MB231 cancer cells. The green-synthesized AgNP-OAL has promising anticancer efficiency that can trigger apoptosis pathway in the MDA_MB231 breast cancer cells.

In Vivo and In Vitro Measurement of Apoptosis in Breast Cancer Cells Using99mTc-EC-Annexin V

2001 ◽  
Vol 16 (1) ◽  
pp. 73-83 ◽  
Author(s):  
David J. Yang ◽  
Ali Azhdarinia ◽  
Peng Wu ◽  
Dong-Fang Yu ◽  
Wayne Tansey ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Annexin V

scholarly journals Development of Functionalized silver Nanoparticles from Allamanda neriifolia Hook and their In Vitro Cytotoxic Effect on MCF-7 Cells

YMER Digital ◽  
2021 ◽  
Vol 20 (12) ◽  
pp. 385-396
Author(s):  
Sumathi R ◽  
◽  
Sivagamasundari K ◽  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Morphological Changes ◽  
Total Phenolic ◽  
Biosynthesized Agnps ◽  
Average Size ◽  
Mcf 7

The present work demonstrates the influence of plant extract composition (antioxidant and total phenolic content) on the size and morphology of the produced AgNPs. In this study, silver nanoparticles (AgNPs) were synthesized using aqueous flower extract of Allamanda neriifolia plant. The biosynthetic procedure was rapid and simple and was easily monitored via colour changes and examined AgNPs (AN-AgNPs) by ultraviolet-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). The results obtained from various characterizations revealed that average size of synthesized AgNPs was 50 nm and in spherical structure. The anticancer potential of AN-AgNPs was investigated against human breast cancer cells (MCF-7). The cytotoxic response was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and morphological changes by apoptosis. The biosynthesized AgNPs-induced cell death in MCF- 7 cells suggested the anticancer potential of AN-AgNPs. Therefore, they may be used to treat the breast cancer cells.

scholarly journals Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 430 ◽  
Author(s):  
Balashanmugam Pannerselvam ◽  
Prabhu Durai ◽  
Devasena Thiyagarajan ◽  
Hak Jin Song ◽  
Kwang Jin Kim ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Human Cancer ◽  
Ultra Violet ◽  
Annexin V ◽  
Spherical Shape ◽  
Human Breast ◽  
Particle Size Range ◽  
Synthesis Of Nanoparticles

Cancer is one of the most dangerous threats to human health and possibly the utmost task for current medicine. Currently, bio-based synthesis of nanoparticles from plants has gained much interest due to its potential medicinal applications. In the present study, a biological approach was employed for biogenic (green) synthesis of silver nanoparticles (AgNPs) using dried leaf extract of Asian spider flower (Asf). The biogenic synthesis of Asf-AgNPs (Asian spider flower-Silver nanoparticles) was established using ultra violet-visible (UV-vis) spectra which exhibited a wide superficial plasmon resonance of AgNPs at 445 nm. These nanoparticles clearly showed the formation of poly-disperse crystalline solids (spherical shape) with particle size range of <50 nm based on observation under a transmission electron microscope (TEM). Infrared spectroscopy (FTIR) revealed carboxylic acids (C = O stretch) known to act as a capping agent and a reductant in plant extracts. Elemental silver signal peak was observed in the graph obtained from energy-dispersive X-ray (EDX) analysis. Biocompatibility tests for Asf-AgNPs at different doses were evaluated against human breast cancer cells (MCF7) for cell viability and apoptotic analysis. According to the evaluation, biosynthesized Asf-AgNPs could prevent the explosion of human breast tumor cells (MCF7) in IC50 at a dose of 40 μg/mL after 48 h of treatment. The results obtained in the IC50 dosage treatments were statistically significant (p < 0.05) when compared with control. Nuclear damage of cells was further investigated using annexin V-FITC/PI dual staining and DAPI (4′,6-diamidino-2-phenylindole) staining method. Bright blue fluorescence with condensed and fragmented chromatin was observed. Western blot analysis showed increased expression levels of caspases-3 and 9 (apoptotic proteins). These results indicate that bio-approached AgNPs synthesized through Asf plant extract could be used as potential therapeutic medications for human cancer cells.

scholarly journals ID:2024 Synthesis and Characterization of PLGA-PEG Thymoquinone Nanoparticle and its Cytotoxicity Effects in Tamoxifen-resistant Breast Cancer Cells

2017 ◽  
Vol 4 (S) ◽  
pp. 55 ◽  
Author(s):  
Rozaina Ahmad ◽  
Noor Haida Mohd Kaus ◽  
Shahrul Hamid
Keyword(s):  
Breast Cancer ◽  
Drug Release ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Volatile Oil ◽  
Pluronic F68

Background: Drug resistance has been a continuous challenge in cancer treatment. The use of nanotechnology in the development of new cancer drugs has potential. One of the extensively studied compound is thymoquinone (TQ) and this work aims to compare two types of TQ-nanoformulation and its cytotoxicity towards resistant cancer cells. Methods: TQ-nanoparticles were prepared and optimized by using 2 different formulation with different drug to PLGA-PEG ratio (1:20 and 1:7) and different PLGA-PEG to pluronic F68 ratio (10:1 and 2:1). The morphology and size were determined using TEM and DLS. Characterization of particles were done using UV-VIS, ATR-IR, entrapment efficiency and drug release. The effects of drug, polymer and surfactants were compared between the two formulations. Cytotoxicity assay was performed using MTS assay. Results: TEM finding showed 96% of particles produced with 1:7 drug to PLGA-PEG were less than 90 nm in size and spherical in shape. This was confirmed with DLS which showed smaller particle size than those formed with 1:20 drug to PLGA-PEG ration. Further analysis showed zeta potential was negatively charge which could facilitate cellular uptake as reported previously. In addition, PDI value was less than 0.1 in both formulations indicating monodispersed and less broad in size distribution. The absorption peak of PLGA-PEG-TQ-Nps were at 255 nm. The 1:7 drug to polymer formulation was selected for further analysis where the entrapment efficiency was 79.9% and in vitro drug release showed a maximum release of TQ at 64% respectively. Cytotoxicity result showed IC50 of TQ-nanoparticle at 20.05 µM and free TQ was 8.25 µM. Conclusion: This study showed that nanoparticle synthesized with 1:7 drug to PLGA-PEG ratio and 2:1 PLGA-PEG to pluronic F68 were less than 100 nm and had spherical shape as confirmed with DLS. This could facilitate its transportation and absorption to reach its target. There was conserved TQ stability as exhibited by slow release of this volatile oil. Cytotoxicity effect was noted when resistant breast cancer cells were treated with these particles.

Cytotoxic effect of green synthesized silver nanoparticles in MCF7 and MDA-MB-231 human breast cancer cells in vitro

The Nucleus ◽  
2019 ◽  
Vol 63 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Arindam Bandyopadhyay ◽  
Bishnupada Roy ◽  
Pallab Shaw ◽  
Paritosh Mondal ◽  
Maloy Kr. Mondal ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Cytotoxic Effect ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Green Synthesized Silver Nanoparticles
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