Design, Optimization and in vitro Characterization of Dasatinib loaded PLGA Nano carrier for Targeted cancer therapy: A Preliminary Evaluation

Objective: Drug nanoparticles offer a versatile platform for enhancing the dissolution rate and bioavailability of poorly water soluble drugs The present study was aimed to design and develop dasatinib (DAS) loaded Poly lactide co glycolic acid (PLGA) to enhance the dissolution rate and to study the effect of formulation variables for the BCS class II drug dasatinib for the treatment of chronic myeloid leukemia. Methods: The DAS loaded Nps were prepared by using modified double emulsion solvent evaporation method (DESE) using different stabilizers, the formulated Nps were characterized for particle size, zeta potential, Poly Dispersity Index, Surface morphology, Drug entrapment and Invitro drug release. Results: The DAS loaded NP s showed the lowest particles size of 123 nm and zeta potential of – results of Pluronic F68 loaded NP showed the lowest particle size of – and highest zeta potential of --. Surface morphology of NPs with DMAB showed distinct smooth spherical particles with the size range of 50nm. Morphology of Pluronic F68 formulated NPs showed the high degree of aggregation. In vitro drug release showed up to 24hrs in a sustained manner. Conclusion: The result of our study indicates the use of PLGA as a sustained release polymer and using DMAB as a stabilizer for better stable formulation.

Chemical stabilization of dispersed Escherichia coli for enhanced recovery with a handheld electroflotation system and detection by Loop-mediated Isothermal AMPlification

Constraints related to sample preparation are some of the primary obstacles to widespread deployment of molecular diagnostics for rapid detection of trace quantities (≤103 CFU/mL) of food-borne pathogens. In this research, we report a sample preparation method using a novel handheld electroflotation system to concentrate and recover dilute quantities (102−103 CFU/mL) of Escherichia coli (E. coli) 25922 in artificially contaminated samples for reliable, rapid detection by loop-mediated isothermal amplification (LAMP). To protect suspended cells from shear stresses at bubble surfaces, a non-ionic surfactant (Pluronic-F68) and flocculant (chitosan oligosaccharide) were used to aggregate cells and reduce their surface hydrophobicity. Effective conditions for recovery were determined through multifactorial experiments including various concentrations of Pluronic-F68 (0.001, 0.01, 0.1, 1 g L-1), chitosan oligosaccharide (0.01, 0.1, 1, 10 g L-1), bacteria (102, 103, 104 CFU/mL E. coli 25922), recovery times (10, 15 and 20 minutes), and degrees of turbulent gas flux (“high” and “low”). The automated electroflotation system was capable of concentrating effectively all of the bacteria from a large sample (380 mL 0.1 M potassium phosphate buffer containing 102 CFU/mL E. coli) into a 1 mL recovered fraction in less than 30 minutes. This enabled detection of bacterial contaminants within 2 hours of collecting the sample, without a specialized laboratory facility or traditional enrichment methods, with at least a 2–3 order of magnitude improvement in detection limit compared to direct assay with LAMP.

Effect of Pluronic-F68 fog solution on performance and morbidity of newly received heifer calves

Morbidity and mortality from bovine respiratory disease (BRD) of newly received feedlot cattle continue to be problems for the feedlot industry. The objective of this study was to evaluate the effects of utilizing a novel breathing treatment containing a nonionic surfactant (Pluronic-F68) on performance and morbidity of high-risk calves during a 45-d receiving period. Angus/Angus-cross heifer calves (n = 240) were acquired in Delhi, LA, and transported (14 h) to the research facility. Heifers were allowed 21-h rest with access to water and RAMP prior to processing. Heifers were sorted into 16 pens by processing order and randomized by pen into one of two treatments: novel breathing treatment containing 6.25% Pluronic-F68 solution, 28.13% glycerin, and 65.62% water (FOG; n = 8 pens per treat and 15 heifers per pen) and control (CON; n = 8 pens per treat and 15 heifers per pen). Control heifers were held in an enclosed stock trailer for 10 min and followed by FOG heifers, during which time treatment was administered. The person responsible for identifying signs of morbidity was blinded to treatment assignments. Data were analyzed as a completely randomized design using MIXED (continuous) or GLIMIX (binomial) models of SAS 9.4. Average daily gain was similar between treatments (P = 0.91). No differences were found in dry matter intake (P = 0.14) nor in gain efficiency (P = 0.58). There were no differences (P = 0.74) in final body weights. Morbidity was similar at first, second, and third antimicrobial administration regardless of treatment (P ≥ 0.34). The number of antimicrobial treatments required or the management of BRD was similar between treatments (P = 0.72). There was no difference (P = 0.44) in mortality between FOG and CON groups. The Pluronic-F68 solution did not improve performance or reduce morbidity of newly received heifer calves; however, further research with a different concentration and/or duration of fogging may be warranted.

PSI-28 Effect of Pluronic-F68 fog solution on performance and morbidity of newly-received heifer calves

Morbidity and mortality from bovine respiratory disease of newly-received feedlot cattle continues to be a problem for the feedlot industry. The objective of this study was to evaluate effects of utilizing a novel breathing treatment containing a non-ionic surfactant (Pluronic-F68) on performance and morbidity of high-risk calves during a 45-d receiving period. Angus/Angus-cross heifer calves (n = 240) were acquired in Delhi, LA and transported (14h) to the research facility. Heifers were allowed 21-h rest with access to water and RAMP® prior to processing. Heifers were sorted into 16 pens by processing order and randomized into one of two treatments: novel breathing treatment containing 6.25% Pluronic-F68 solution, 28.13% glycerin, and 65.62% water (FOG; n = 8 pens/treat and 15 heifers/pen) and control (CON; n = 8 pens/treat and 15 heifers/pen). Control heifers were held in an enclosed stock trailer for 10 min and followed by FOG heifers, during which time treatment was administered. The person responsible for identifying signs of morbidity was blinded to treatment assignments. Data were analyzed as a completely randomized design using MIXED (continuous) or GLIMMIX (binomial) models of SAS 9.4. Average daily gain was similar between treatments (P = 0.91). No differences were found in dry matter intake (P = 0.14) nor in feed efficiency (P = 0.58). There were no differences (P = 0.74) in final body weights. Morbidity was similar at first, second and third antimicrobial administration regardless of treatment (P ≥ 0.34). The number of antimicrobial treatments required or the management of BRD was similar between treatments (P = 0.72) There was no difference (P = 0.44) in mortality between FOG and CON groups. The Pluronic-F68 solution did not improve performance or reduce morbidity of newly received heifer calves; however, further research with a different concentration and/or duration of fogging may be warranted.

Effect of Hyaluronic Acid and Pluronic-F68 on the Surface Properties of Foam as a Delivery System for Polidocanol in Sclerotherapy

The use of foams to deliver bioactive agents and drugs is increasing in pharmaceutics. One example is the use of foam as a delivery system for polidocanol (POL) in sclerotherapy, with the addition of bioactive compounds to improve the delivery system being a current subject of study. This work shows the influence of two bioactive additives on the structure and stability of POL foam: hyaluronic acid (HA) and Pluronic-F68 (F68). HA is a natural non-surface-active biopolymer present in the extracellular matrix while F68 is a surface-active poloxamer that is biocompatible with plasma-derived fluids. Both additives increase the bulk viscosity of the sample, improving foam stability. However, HA doubled and F68 quadruplicated the foam half lifetime of POL. HA reduced the size and polydispersity of the bubble size distribution and increased the surface elasticity with respect to POL. Both facts have a positive impact in terms of foam stability. F68 also altered bubble structure and increased surface elasticity, again contributing to the enhancement of foam stability. The surface characterization of these systems is important, as in foam sclerotherapy it is crucial to assure the presence of POL at the surface of the bubbles in order to deliver the sclerosant agent in the target vein.

FORMULASI DAN KARAKTERISASI NANOSUSPENSI MORIN DENGAN METODE SONOPRESIPITASI

Pendahuluan : Morin (3,5,7, 2,4’- pentahidroksiflavone) adalah senyawa flavonoid golongan flavonol yang poten sebagai antioksidan, antikanker, antiinflamasi dan sebagai antidiabetes. Morin bersifat lipofil, tidak larut dalam air sehingga ketersediaan hayati dan aplikasi klinis menjadi terbatas. Nanosuspensi adalah sistem dispersi koloidal, 100% mengandung bahan obat dengan ukuran 10-1000 nm, tidak mengandung bahan pembawa apapun kecuali sebagai bahan penstabil. Tujuan : Penelitian ini bertujuan membuat nanosuspensi morin yang stabil dengan metode sonopresipitasi. Karakterisasi nanosuspensi meliputi ukuran partikel, indeks polidispersitas, zeta potensial, morfologi nanosuspensi dan aktivitas antioksidan. Metode : Nanosuspensi morin dibuat dengan metode sonopresipitasi dengan frekuensi 50 kHz selama 8 menit dengan variasi bahan penstabil jenis surfaktan, polimer dan kombinasi keduanya. Karakterisasi nanosuspensi meliputi ukuran partikel, indeks polidispersitas, zeta potensial, morfologi partikel (TEM), stabilitas fisik dan aktivitas antioksidan. Kesimpulan : Morin berhasil dikembangkan menjadi nanosuspensi dengan metode sonopresipitasi. Formula 7 dengan stabilizer SLS-Pluronic F68 (0,5:1) merupakan formula terpilih dengan ukuran partikel terkecil sebesar 182,7 nm, ukuran partikel seragam (PI : 0,210), stabil setelah penyimpanan 8 minggu dengan nilai zeta potensial -37,8 mV. Aktivitas antioksidan nanosuspensi morin meningkat sebesar 2-3 kali

Formulation, Development and Evaluation of Lopinavir Loaded Polymeric Micelles

Lopinavir is the anti HIV drug which is used to treat the HIV-1 infection. In this study we used the single lopinavir drug to formulate the polymeric micelle. This study was done with the two main objectives as Objective:first to enhance the solubility and bioavailability of the BCS class IV drug and second to avoid the combination of lopinavir rand ritonavir and use single lopinavir to preparation of polymeric micelle also to avoid the disadvantages related to the oral administration. Method:The different pluronic (F188 &F127) and co-solvent (Tween80) were chosen & the micelles were prepared by using different Drug: polymer ratio with or without cosolvent and drug Lopinavir. Formulations were been characterized by critical micelle concentration(CMC) value, micellesize,DSC, XRD, loading efficiency, % drug loading and stability.Result:Mixed micelle (hydrophobic &hydrophilic) obtained from optimized batch shows the highest entrapment of 29%with the pluronic F68 with the use of co-solvent and the vesicle size of 0.156µm the DSC, FTIR, XRD study was also done for lopinavir and optimized formulation .Conclusion: The pluronic F68 with the co-solvent showed fairly high entrapment efficiency, loading capacity than the mixed Pluronic in combination