scholarly journals Identification of Mycoplasma Species and Related Organisms From Ruminants in England and Wales During 2005 – 2019

2021 ◽  
Author(s):  
Alannah S. Deeney ◽  
Rachael Collins ◽  
Anne M. Ridley
Keyword(s):  
Respiratory Disease ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Animal Health ◽  
Small Ruminants ◽  
Health Agency ◽  
Mycoplasma Species ◽  
Rrna Gene ◽  
Contagious Bovine Pleuropneumonia ◽  
England And Wales ◽  
The Uk

Abstract Background: Mycoplasma species have been associated with economically important diseases affecting ruminants worldwide and include contagious bovine pleuropneumonia (CBPP), contagious caprine pleuropneumonia (CCPP) and contagious agalactia, listed by the World Organisation for Animal Health (OIE). The Mycoplasma Team at the Animal and Plant Health Agency provides an identification service for Mycoplasma and Ureaplasma species of veterinary importance to the United Kingdom (UK), supporting the detection of new and emerging pathogens, as well as contributing to the surveillance of endemic, and the OIE listed diseases exotic to the UK. Mycoplasma and other Mollicutes species were identified from diagnostic samples from farmed ruminants in England and Wales using a combination of culture and 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis, submitted between 2005 and 2019.Results: A total of 5578 mollicutes identifications, which include mycoplasmas and the related acholeoplasmas and ureaplasmas, were made from farmed ruminant animals during the study period. Throughout the study period, the pathogen Mycoplasma bovis was consistently the most frequently identified species, accounting for 1411 (32%) of 4447 molecular identifications in cattle, primarily detected in the lungs of pneumonic calves, followed by joints and milk of cattle showing signs of arthritis and mastitis, respectively. M. bovirhinis, M. alkalescens, M. dispar, M. arginini and Ureaplasma diversum, were also common. Mixed species, principally M. bovis with M. alkalescens, M. arginini or M. bovirhinis were also prevalent, particularly from respiratory samples. The non-cultivable blood-borne haemoplasmas Candidatus ‘Mycoplasma haemobos’ and Mycoplasma wenyonii were identified from cattle, with the latter species most often associated with milk-drop. M. ovipneumoniae was the predominant species identified from sheep and goats experiencing respiratory disease, while M. conjunctivae preponderated in ocular samples. The UK remains free of the ruminant mycoplasmas listed by OIE. Conclusions: The continued high prevalence of M. bovis identifications confirms its ongoing dominance and importance as a significant pathogen of cattle in England and Wales, particularly in association with respiratory disease. M. ovipneumoniae has seen a general increase in prevalence in recent years, notably in coughing lambs and should therefore be considered as a primary differential diagnosis of respiratory disease in small ruminants.

scholarly journals Identification of Mycoplasma species and related organisms from ruminants in England and Wales during 2005–2019

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Alannah S. Deeney ◽  
Rachael Collins ◽  
Anne M. Ridley
Keyword(s):  
Respiratory Disease ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Animal Health ◽  
Small Ruminants ◽  
Health Agency ◽  
Mycoplasma Species ◽  
Rrna Gene ◽  
Contagious Bovine Pleuropneumonia ◽  
England And Wales ◽  
The Uk

Abstract Background Mycoplasma species have been associated with economically important diseases affecting ruminants worldwide and include contagious bovine pleuropneumonia (CBPP), contagious caprine pleuropneumonia (CCPP) and contagious agalactia, listed by the World Organisation for Animal Health (OIE). The Mycoplasma Team at the Animal and Plant Health Agency provides an identification service for Mycoplasma and Ureaplasma species of veterinary importance to the United Kingdom (UK), supporting the detection of new and emerging pathogens, as well as contributing to the surveillance of endemic, and the OIE listed diseases exotic to the UK. Mycoplasma and other Mollicutes species were identified from diagnostic samples from farmed ruminants in England and Wales using a combination of culture and 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis, submitted between 2005 and 2019. Results A total of 5578 mollicutes identifications, which include mycoplasmas and the related acholeoplasmas and ureaplasmas, were made from farmed ruminant animals during the study period. Throughout the study period, the pathogen Mycoplasma bovis was consistently the most frequently identified species, accounting for 1411 (32%) of 4447 molecular identifications in cattle, primarily detected in the lungs of pneumonic calves, followed by joints and milk of cattle showing signs of arthritis and mastitis, respectively. M. bovirhinis, M. alkalescens, M. dispar, M. arginini and Ureaplasma diversum, were also common. Mixed species, principally M. bovis with M. alkalescens, M. arginini or M. bovirhinis were also prevalent, particularly from respiratory samples. The non-cultivable blood-borne haemoplasmas Candidatus ‘Mycoplasma haemobos’ and Mycoplasma wenyonii were identified from cattle, with the latter species most often associated with milk-drop. M. ovipneumoniae was the predominant species identified from sheep and goats experiencing respiratory disease, while M. conjunctivae preponderated in ocular samples. The UK remains free of the ruminant mycoplasmas listed by OIE. Conclusions The continued high prevalence of M. bovis identifications confirms its ongoing dominance and importance as a significant pathogen of cattle in England and Wales, particularly in association with respiratory disease. M. ovipneumoniae has seen a general increase in prevalence in recent years, notably in coughing lambs and should therefore be considered as a primary differential diagnosis of respiratory disease in small ruminants.

scholarly journals Equine piroplasmosis status in the UK: an assessment of laboratory diagnostic submissions and techniques

2018 ◽  
Vol 184 (3) ◽  
pp. 95-95 ◽  
Author(s):  
Robert M Coultous ◽  
Paul Phipps ◽  
Charlie Dalley ◽  
Jane Lewis ◽  
Toni-Ann Hammond ◽  
...  
Keyword(s):  
Animal Health ◽  
Health Agency ◽  
Clinical Samples ◽  
Vector Species ◽  
Equine Piroplasmosis ◽  
Tick Vector ◽  
Increased Risk ◽  
The Uk ◽  
Tick Vectors ◽  
Health Trust

Equine piroplasmosis (EP) has historically been of minor concern to UK equine practitioners, primarily due to a lack of competent tick vectors. However, increased detection of EP tick vector species in the UK has been reported recently. EP screening is not currently required for equine importation, and when combined with recent relaxations in movement regulations, there is an increased risk regarding disease incursion and establishment into the UK. This study evaluated the prevalence of EP by both serology and PCR among 1242 UK equine samples submitted for EP screening between February and December 2016 to the Animal and Plant Health Agency and the Animal Health Trust. Where information was available, 81.5 per cent of submissions were for the purpose of UK export testing, and less than 0.1 per cent for UK importation. Serological prevalence of EP was 8.0 per cent, and parasite DNA was found in 0.8 per cent of samples. A subsequent analysis of PCR sensitivity in archived clinical samples indicated that the proportion of PCR-positive animals is likely to be considerably higher. The authors conclude that the current threat imposed by UK carrier horses is not adequately monitored and further measures are required to improve national biosecurity and prevent endemic disease.

scholarly journals A spatial analysis of lime resources and their potential for improving soil magnesium concentrations and pH in grassland areas of England and Wales

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
T. Bide ◽  
E. L. Ander ◽  
M. R. Broadley
Keyword(s):  
Soil Ph ◽  
Production Systems ◽  
Animal Health ◽  
British Geological Survey ◽  
England And Wales ◽  
North East ◽  
Effective Manner ◽  
Potential Demand ◽  
The Uk ◽  
On Farm

AbstractMagnesium (Mg) is essential for animal health. Low Mg status (hypomagnesaemia) can be potentially fatal in ruminants, like cattle and sheep, and is widespread in Europe with economic impacts on farming. The application of Mg-rich agricultural lime products can help to ensure pasture forage consumed by animals contains sufficient Mg and, in areas of low pH, has the dual benefit of reducing soil acidity to levels best suited for grass production. This aim of this study was to determine if Mg-rich lime products could be used in a more effective manner in agricultural production systems. Potential resources of carbonate rocks (limestone, dolostone and chalk) in the UK, and their Mg:Ca status were identified, using datasets from the British Geological Survey (BGS). These data were combined with the locations of agricultural lime quarries, and areas where soils are likely to be deficient in Mg and/or require liming. Areas of potential demand for Mg-rich agricultural lime include areas in south east Wales, the Midlands and North East England. Although, areas where this may be an effective solution to low soil Mg values are restricted by the availability of suitable products. Conversely, areas of low soil pH in England and Wales are often found close to quarries with the ability to supply high Ca limes, suggesting that the low rates of lime use and liming is not due to supply factors. This study provides information that can help to guide on-farm decision making for use of Mg-rich and other lime resources. This could be used in conjunction with other options to reduce risks of Mg deficiency in livestock, and improve soil pH.

Detection of Mycoplasma gallinaceum by PCR amplification of the 16S rRNA gene from respiratory disease in village chickens

2018 ◽  
Author(s):  
P. Ponnusamy ◽  
T. Lurthu Reetha ◽  
B.S.M. Ronald ◽  
B. Puvarajan and R. Manicakm
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Respiratory Disease ◽  
Tamil Nadu ◽  
Abdominal Cavity ◽  
Pcr Amplification ◽  
Mycoplasma Species ◽  
Rrna Gene ◽  
Village Chickens ◽  
The 16S Rrna Gene

In the present study, Mycoplasma gallinaceum was detected by PCR amplification of 16S rRNA gene from chronic respiratory disease in village chickens of Cauvery delta region of Tamil Nadu. Necropsy was performed to find out the etiological agent in desi birds mortality. At necropsy, airsacculitis with caseous exudate were found in the thoracic and abdominal cavity. Caseous material from airsacs was collected aseptically from dead birds for detection of Mycoplasma species. DNA was extracted from caseous material by using tissue DNA extraction kit. PCR was carried out using primers to amplify 16S rRNA gene belonging to Mycoplasma species. The amplified product yielded approximately 700-bp length (703 to 713 bp) of the 16S rRNA gene specific for Mycoplasma species. Further, it was subjected to sequence analysis and confirmed as Mycoplasma gallinaceum by NCBI blast analysis. In the present communication, detection of M. gallinaceum by PCR amplification of 16S rRNA gene provides a powerful tool for rapid diagnosis. 

scholarly journals A Spatial Analysis of Lime Resources and their Potential for Improving Soil Magnesium Concentrations and pH in Grassland Areas of England and Wales

2021 ◽  
Author(s):  
T. Bide ◽  
E. L. Ander ◽  
M. R. Broadley
Keyword(s):  
Soil Ph ◽  
Production Systems ◽  
Animal Health ◽  
British Geological Survey ◽  
England And Wales ◽  
North East ◽  
Effective Manner ◽  
Potential Demand ◽  
The Uk ◽  
On Farm

Abstract Magnesium (Mg) is essential for animal health. Low Mg status (hypomagnesaemia) can be potentially fatal in ruminants, like cattle and sheep, and is widespread in Europe with economic impacts on farming. The application of Mg-rich agricultural lime products can help to ensure pasture forage consumed by animals contains sufficient Mg and, in areas of low pH, has the dual benefit of reducing soil acidity to levels best suited for grass production. This aim of this study was to determine if Mg-rich lime products could be used in a more effective manner in agricultural production systems. Potential resources of carbonate rocks (limestone, dolostone and chalk) in the UK, and their Mg:Ca status were identified, using datasets from the British Geological Survey (BGS). These data were combined with the locations of agricultural lime quarries, and areas where soils are likely to be deficient in Mg and/or require liming.Areas of potential demand for Mg-rich agricultural lime include areas in south east Wales, the Midlands and North East England. Although, areas where this may be an effective solution to low soil Mg values are restricted by the availability of suitable products. Conversely, areas of low soil pH in England and Wales are often found close to quarries with the ability to supply high Ca limes, suggesting that the low rates of lime use and liming is not due to supply factors.This study provides information that can help to guide on-farm decision making for use of Mg-rich and other lime resources. This could be used in conjunction with other options to reduce risks of Mg deficiency in livestock, and improve soil pH.

TBE in United Kingdom

2020 ◽  
Keyword(s):  
United Kingdom ◽  
Clinical Disease ◽  
England And Wales ◽  
Probable Case ◽  
The United Kingdom ◽  
South West ◽  
Louping Ill ◽  
The Uk ◽  
North And South ◽  
South West England

Until 2019, TBE was considered only to be an imported disease to the United Kingdom. In that year, evidence became available that the TBEV is likely circulating in the country1,2 and a first “probable case” of TBE originating in the UK was reported.3 In addition to TBEV, louping ill virus (LIV), a member of the TBEV-serocomplex, is also endemic in parts of the UK. Reports of clinical disease caused by LIV in livestock are mainly from Scotland, parts of North and South West England and Wales.4

HIV and other STIs in the United Kingdom: further increases

2003 ◽  
Vol 7 (48) ◽  
Author(s):  
◽  
Keyword(s):  
United Kingdom ◽  
Disease Surveillance ◽  
Communicable Disease ◽  
People Living With Hiv ◽  
Health Protection Agency ◽  
Protection Agency ◽  
England And Wales ◽  
The United Kingdom ◽  
The Uk ◽  
Living With Hiv

The Health Protection Agency Communicable Disease Surveillance Centre for England and Wales and others have reported that the number of people living with HIV in the UK has increased

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