scholarly journals BA and TDZ in the Morphogenesis in Vitro of Passiflora Setacea

2022 ◽  
Author(s):  
Leticia da Silva Araújo ◽  
Virginia Silva Carvalho ◽  
Andressa Leal Generoso ◽  
Josefa Grasiela Silva Santana ◽  
Glaziele Campbell ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Indirect Organogenesis ◽  
In Vitro Morphogenesis ◽  
Important Species ◽  
Genetic Breeding ◽  
Morphogenesis In Vitro

Abstract Passiflora setacea DC (Passifloraceae) is considered an important species in the genetic breeding of passion fruit. However, its use is limited due to low seed germination. This paper aimed to study the effect of cytokinins 6-benzyladenine (BA) and thidiazuron (TDZ) on the in vitro morphogenesis of P. setacea using three explants: hypocotyl, nodal segment, and root segment. The explants were induced to morphogenesis in MS medium modified and with different concentrations of BA and TDZ. After 55 days, the percentage of calluses and shoots were evaluated, and anatomical characterization was performed. The three explants used in the in vitro morphogenesis of P. setacea showed callus and shoots formation, but in greater numbers in the nodal segments treated with BA. TDZ isolated affected equal to or less than BA on callus and shoots formation for the three explants. Direct and indirect organogenesis was observed in the three types of explants. From the results obtained for plant regeneration via in vitro morphogenesis of P. setacea, it is recommended to use a nodal segment in MSM medium and supplemented with 2.22 μmol L-1 of BA.

scholarly journals Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (Aegle marmelos L.)

1970 ◽  
Vol 44 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Ranjoy Das ◽  
M Faruk Hasan ◽  
Harunar Rashid ◽  
Motiur Rahman
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Nodal Explant ◽  
Aegle Marmelos ◽  
Half Strength ◽  
Subsequent Regeneration

This study reports on an improved protocol for callus induction and subsequent regeneration from nodal segment of wood apple (Aegle marmelos L.) Creamish friable competent callus was achieved from nodal segments on MS medium augmented with 4.0 mg1-1 2,4-D within two weeks of inoculation. The callus produced large number of shoots when cultured on MS medium fortified with 2.0 mgl-1 BAP+0.1 mgl-1 NAA within ten days of culture. In vitro raised shoots were rooted on half strength MS medium enriched with 1.0 mgl-1 IBA within fifteen days of culture. The rooted plantlets were successfully established with 80% survival. Key words: Plant regeneration; Callus induction; Nodal explant; Aegle marmelos. DOI: 10.3329/bjsir.v44i4.4590 Bangladesh J. Sci. Ind. Res. 44(4), 415-420, 2009

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Physalis minima L. - a Perennial Medicinal Herb

2010 ◽  
Vol 44 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Shoot Tip Explants

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate. Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration DOI: 10.3329/bjsir.v44i4.4597 Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

scholarly journals Callus induction and plant regeneration of paederia foetida L., a widely used medicinal vine in Bangladesh

2013 ◽  
Vol 47 (4) ◽  
pp. 373-378
Author(s):  
AKMS Hassan ◽  
CK Roy ◽  
R Sultana ◽  
R Khatun
Keyword(s):  
Plant Regeneration ◽  
Leaf Shape ◽  
Basal Medium ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Nodular Callus ◽  
Paederia Foetida

An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obtained when the callus was sub cultured on MS medium with 0.5 mg/L BAP. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/L IBA. The survival rate of plantlets was found to be 85%. Regenerated plants were morphologically uniform having normal leaf shape and growth. Bangladesh J. Sci. Ind. Res. 47(4), 373-378, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.14066

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

scholarly journals Effect of Environmental Factors on Successful in vitro Morphogenesis of Madhuca longifolia

2020 ◽  
pp. 9-19
Author(s):  
Fatima Shirin ◽  
Deepti Bhadrawale ◽  
Sonam Singh ◽  
Shalu Panika ◽  
Trilok Gupta
Keyword(s):  
Effective Means ◽  
Shoot Multiplication ◽  
Nodal Segments ◽  
Soap Industry ◽  
Ms Medium ◽  
Step Method ◽  
In Vitro Morphogenesis ◽  
Madhuca Longifolia ◽  
Commercially Important

Madhuca longifolia is a commercially important tree species commonly known as mahua. The livelihood of large populations of tribal people depends on collection of its flowers and seeds. Almost all the parts of Mahua are utilized in diversified uses like in industry as artificial sweetner, biodiesel, food products, in soap industry etc. In the present study, a successful attempt was made to establish in vitro cultures of Mahua from nodal segments and factors influencing in vitro morphogenesis were evaluated as propagation through seeds and cuttings encounters problems. Axillary bud break (64.44%) was successfully achieved by culturing nodal segments on Murashige and Skoogs (MS) medium supplemented with 3 mg l-1Benzyladenine (BA) in nodal explants collected during the months ofJuly-September (rainy season).Shoot multiplication with maximum number of shoots, maximum number of leaves and longest shoots was achieved on MS medium supplemented with 3 mg l-1 BA when a subculture cycle of 30 days was followed. On MS medium supplemented with 2 mg l-1 Indole-3-Butyric Acid (IBA), in vitro excised shoots were successfully rooted (55.55%) after 40 days. A two step method was employed for successful hardening of rooted plantlets. Firstly, the plantlets were transferred for one week in 1/2 strength of MS liquid medium. Then, the plantlets were transferred to root trainers containing soilrite soaked with inorganic salts of ½ strength MS medium. The hardened plantlets were acclimatized firstly in a mist chamber and then in polybags in shade house. The present study provides an effective means for in vitro shoot regeneration and plantlet formation through nodal segments of Madhuca longifolia, a commercially important tropical tree with multifarious uses.

scholarly journals In vitro micropropagation of Rauvolfia serpentina (L.) Benth through induction of direct and indirect organogenesis

2013 ◽  
pp. 01-09
Author(s):  
SK Bhadra ◽  
TK Bhowmik ◽  
P Singh
Keyword(s):  
Callus Tissue ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Indirect Organogenesis ◽  
Rauvolfia Serpentina ◽  
Shoot Buds ◽  
Wide Range ◽  
Multiple Shoot Buds

Leaf and nodal segments of two months old field grown seedlings of Rauvolfia serpentina (L.) Benth were aseptically cultured on agar solidified MS medium supplemented with various combinations and concentrations of auxins (NAA, IAA, 2,4-D and picloram) and cytokinins (BAP and Kn). The nodal segments produced highest number of multiple shoot buds (5.85/explant) on MS medium supplemented with 2.0 mgl-1 BAP + 0.2 mgl-1 NAA or 2.0 mgl-1 BAP + 0.1 mgl-1 IAA. Whereas nodal segment produced callus tissue of different nature on MS medium supplemented with 1.5 mgl-1 BAP + 0.5 mgl-1 IAA+ 1.5 mgl-1 2,4-D; 3.0 mgl-1 BAP + 1.0 mgl-1 NAA + 1.5 mgl-1 Kn and 0.1 mgl-1 Pic + 1.0 mgl-1 Kn. The callus tissue of light green and nodular nature on further subculture in a wide range of plant growth regulators (PGRs) supplemented media, differentiated into multiple shoot buds that underwent rapid elongation on 2.0 mg/l BAP and 0.2 mg/l NAA supplemented media. The elongated shoot buds on further subculture in rooting media produced strong and stout roots. Half strength MS with 1.5% (w/v) sucrose was most effective for enhancing rooting. Finally those plantlets were acclimatized in field. Thus a protocol was established for rapid micropropagation of this medicinal plant through induction of direct and indirect organogenesis from nodal explant. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13401 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):01-09, 2008

scholarly journals Plant Regeneration from Leaf Explants of the Medicinal Herb Wedelia chinensis

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 407
Author(s):  
Yung-Ting Tsai ◽  
Kin-Ying To
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Liver Toxicity ◽  
Leaf Explants ◽  
Basal Medium ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Average Percentage

Wedelia chinensis, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of W. chinensis by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA3), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of W. chinensis in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant W. chinensis.

scholarly journals In vitro Propagation of Abrus precatorius L. - A Rare Medicinal Plant of Chittagong Hill Tracts

1970 ◽  
Vol 17 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Animesh Biswas ◽  
Mohashweta Roy ◽  
MA Bari Miah ◽  
SK Bhadra
Keyword(s):  
Medicinal Plant ◽  
Adventitious Shoots ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Indirect Organogenesis ◽  
Abrus Precatorius ◽  
Nodular Callus ◽  
Half Strength ◽  
Cut Surface

An efficient protocol was developed for in vitro propagation of Abrus precatorius L. through induction of indirect organogenesis in nodal segment derived callus tissue. Yellowish-green nodular callus was induced at the cut surface of the nodal segments cultured on MS fortified with 5.0 mg/l BAP and 0.5 mg/l NAA. The callus differentiated into adventitious shoots when it was subcultured on to MS supplemented with 3.0 mg/l BAP + 0.5 mg/l Kn + 0.5 mg/l NAA. On an average 6.87 ± 0.26 shoots/culture developed. These microshoots were rooted in half-strength MS containing 1.0 mg/l IBA and the rooted plantlets were transferred to soil after proper acclimatization.Key words: Abrus precatorius, Medicinal plant, Callus culture, PropagationDOI = 10.3329/ptcb.v17i1.1121Plant Tissue Cult. & Biotech. 17(1): 59-64, 2007 (June)

scholarly journals Massive in vitro Cloning of Sandalwood (Santalum album Linn.) via Cultured Nodal Segments

2019 ◽  
pp. 1-14
Author(s):  
D. Bele ◽  
Nishi Mishra ◽  
Sushma Tiwari ◽  
M. K. Tripathi ◽  
G. Tiwari
Keyword(s):  
Somatic Embryogenesis ◽  
Plantlet Regeneration ◽  
Nodal Segments ◽  
Direct Somatic Embryogenesis ◽  
Direct Organogenesis ◽  
Santalum Album ◽  
Ms Medium ◽  
Regeneration Medium ◽  
Indirect Organogenesis

Nodal segments of sandalwood were cultured on MS medium amended with different plant growth regulators in varying concentrations to search out higher in vitro response leading to plantlet regeneration via somatic embryogenesis and/or organogenesis. Higher proportion of direct somatic embryogenesis, number(s) of somatic embryo per explant and plantlet regeneration via direct organogenesis were recorded on MS medium supplemented with a moderate concentration of TDZ (1.0 mgl-1) in combination with comparatively a lower concentration of NAA (0.5 mgl-1). A relative higher concentration of BAP (1.0-2.0 mgl-1) in combination with a lower concentration of NAA (0.5 mgl-1) promoted frequency of indirect somatic embryogenesis. Ratio of organ formation directly from surface of cultured explants was recovered from culture medium fortified with a higher concentration of BA at the concentration of 4.0 mgl-1 in combination with a lower concentration of NAA (0.5 mgl-1). Maximum plantlets regenerated via somatic embryogenesis (direct and/or indirect) on regeneration medium supplemented with 2.0 mgl-1TDZ  in combination with 1.0 mg l-1GA3, while plantlets in higher frequencies via indirect organogenesis was attained with regeneration medium amended with comparatively lower concentration of TDZ (1.0 mg l-1) in combination with 0.5 mgl-1 GA3 and 0.5     mgl-1 NAA. The plantlets were transferred to pots and hardened in Environmental Growth Cabinet and Net House during preliminary weaning period and transferred to field successfully. Morphologically normal plants were recovered.

scholarly journals Micropropagation of Asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis

2017 ◽  
Vol 29 (2) ◽  
pp. 143-153
Author(s):  
Anna Pindel
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Ms Medium ◽  
Regeneration System ◽  
Indirect Organogenesis ◽  
Axillary Shoots ◽  
Single Node ◽  
Primary Explants ◽  
Regeneration Response

AbstractThe present study has described a simple protocol for efficient plant regeneration of Asparagus densiflorus ‘Sprengeri’ and ‘Myriocladus’ using single-node spear explants, and indirect organogenesis via callogenesis induced on internode explants. The results showed that the genotypes ‘Sprengeri’ and ‘Myriocladus’ regenerated to complete plants via nodal cultures and callus tissue, but the plant regeneration response was higher in secondary explants on MS medium with NAA + kinetin (1+1 mg dm-3) after transfer onto a multiplication medium with IAA+BAP (1+4 mg dm-3), and then onto a rooting medium supplemented with IBA (10 mg dm-3) or NAA + kinetin (1+1 mg dm-3). Primary explants of both cultivars showed high regenerative potential (via the callus stage) on MS medium with IAA+BAP. The cultivar Sprengeri also regenerated via somatic embryogenesis. Both kinds of ‘Meyeri’ explants have a morphogenetic potential for the formation of shoots, which, however, were not capable of rooting. This confirms that the explant, genotype and culture medium are determining factors in the in vitro plant regeneration system.

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