scholarly journals Assessment of Nutritional And Free Radical Scavenging Activity of Selected Genotypes of Cordia Myxa L.: A Potential Underutilized Fruit Crop of Indian Arid Zone

Author(s):  
Purkha Ram Meghwal ◽  
Dalpat Singh ◽  
Saurabh Swami ◽  
Akath Singh

Abstract Cordia myxa is one of the important underutilized fruit plants suitable for arid and semi-arid regions of India having potential for commercial exploitation in vegetable and pickle industry. Fruits of four improved genotypes of Cordia myxa were analyzed for nutritional value, total phenol content and free radical scavenging activity. Acetone extracts from dried fruit samples were prepared to determine total phenol content and free radical scavenging activity using DPPH essay. The fruits harvested at 20 or 30 days after fruit set recorded higher crude fibre and crude protein as compared to at full ripening stage. The total phenol content was found highest in fruits harvested earliest irrespective of genotypes. The correlation coefficient between total phenol content and free radical scavenging activity of fruit extract was found positive and significant. The results suggest that the fruits of Cordia myxa should be harvested at 20 to 30 days after fruit set to get maximum benefits of its anti-oxidant and other nutritional properties.

2011 ◽  
Vol 3 (2) ◽  
pp. 108-113 ◽  
Author(s):  
Sandhyarani Devi KHOMDRAM ◽  
Potsangbam Kumar SINGH

Eight plants of Lamiaceae under subfamily Nepetoideae found in Manipur, India were selected for estimation of their polyphenolic compounds and free radical scavenging activity which is expressed on dry weight basis. In this present study, the total phenol and flavonoid contents as well as the free radical scavenging activity were studied using spectrophotometric method. The total phenol content was determined based on Folin-Ciocalteau reagent, flavonoid was determined by aluminium chloride spectrophotometric method and tannin by Folin Dennis Method. The free radical scavenging activity was determined by using DPPH radical which is expressed as IC50 (μg/ml). The total phenolic content varied from 21.39±0.927 to 46.28±0.543 mg/g, flavonoids content in the selected samples varied from 13.30±0.684 to 26.03±0.217 mg/g and tannin content varied from 8.72±0.160 to 17.04±0.206 mg/g. The free radical scavenging activity among the selected samples varied from 11.67±0.221 to 38.29±0.532 μg/ml. The correlation between the free radical scavenging activity with total phenol content (R2=0.511), with flavonoids (R2=0.241) and with tannin (R2=0.690) was calculated and maximum correlation value was found between tannin content and the free radical scavenging activity of the plant samples. The result supports that tannins were more responsible for free radical scavenging activity in the presently selected plants.


2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Mathelda Kurniaty Roreng ◽  
Elva Intan Permatasari ◽  
Dolly Citra Manalu ◽  
...  

Akway (Drimys piperita Hook. f) merupakan tumbuhan yang termasuk dalam kelompok tumbuhan berkayu, berdaun tebal aromatik dan termasuk kerabat winteraceae. Tumbuhan ini dimanfaatkan sebagai obat tradisional untuk mengobati malaria dan untuk meningkatkan vitalitas tubuh. Beberapa penelitian kandungan fitokimia ekstrak akway telah dilakukan untuk mengetahui potensi bioaktivitas akway. Ekstrak kulit kayu akway dilaporkan mengandung kelompok senyawa alkaloid, saponin, tanin, flavonoid, terpenoid dan glikosida. Minyak atsiri kulit kayu akway mengandung linalool, β-pinen, α-pinen, nerolidol dan terpineol. Senyawa-senyawa tersebut dilaporkan memiliki aktivitas antioksidan yang tinggi. Tujuan penelitian ini adalah untuk menentukan kapasitas antioksidan ekstrak kulit kayu akway secara in vitro dan vitamin C sebagai kontrol positif. Pengujian kapasitas antioksidan yang dilakukan meliputi kandungan total fenol dengan metode Folin-Ciocalteu, kandungan flavonoid dengan metode aluminum klorida, kapasitas penangkalan radikal bebas menggunakan metode DPPH (1,1-Diphenyl-2-picrylhydrazyl)-radical scavenging assay dan daya reduksi menggunakan metode reduksi Fe+3 menjadi Fe+2. Hasil penelitian menunjukkan bahwa ekstrak metanol memiliki kandungan total fenol dan flavonoid yang tertinggi dibandingkan dengan ekstrak etanol dan etilasetat. Kandungan total fenol dan flavonoid ekstrak metanol masing-masing sebesar 18,22 dan 14,32%. Ekstrak metanol dan vitamin C memiliki kapasitas menangkal radikal bebas DPPH dan daya reduksi yang paling tinggi kemudian diikuti ekstrak etanol dan etilasetat. Kemampuan menangkal radikal bebas ekstrak metanol dan vitamin C pada konsentrasi 200 µg/ml masing-masing sebesar 90% dan 88,31% sedangkan daya reduksi masing-masing sebesar 0,54 dan 0,62. Kesimpulannya, ekstrak metanol memiliki kapasitas antioksidan yang paling tinggi dibandingkan dengan ekstrak etanol dan etilasetat. Abstract Free Radical Scavenging Activity and Reducing Power of Akway (Drimys piperita Hook. f.) Bark ExtractsAkway (Drimys piperita Hook. f) was a woody and aromatic plant of winteraceae. This plant was used as traditional medical plant to heal malaria and to enhance vitality of body. Some studies were done to know bioactivity potency of akway extracts. D. piperita bark extract contains alkaloid, saponin, tanin, flavonoid, terpenoid and glycoside. The bark essential oil of the plant consists of lynalool, β-pinene, α-pinene, nerolidol and terpineol. Those compounds were exhibited high antioxidant activity. The objectives of this research were to determine total phenol and flavonoid of the extracts and its antioxidant capacity which was compared to antioxidant capacity of vitamin C. The assay of antioxidant capacity comprised of total phenol and flavonoid content, free radical scavenging activity, and reducing power. Total phenol and flavonoid was determined using Folin-Ciocalteu and aluminum chloride method, respectively while determination of free radical scavenging activity and reducing power using DPPH-radical scavenging and Fe+3 to Fe+2 reducing power method, respectively. The results indicated that methanol extract had the highest in total phenol and flavonoid content. Total phenol and flavonoid of methanol extract were 18.22% and 14.32%, respectively. Vitamin C and methanol extract had the highest DPPH (1,1-Diphenyl-2-picrylhydrazyl)-radical scavenging activity and reducing power, respectively. DPPH-radical scavenging activity of methanol extract and vitamin C in concentration of 200 µg/ml were 90% and 88.31%, respectively while its reducing power were 0.54 and 0,62, respectively. As conclusion, methanol extract had the highest antioxidant activity compared with ethanol and ethyl acetate extracts.


Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 165-171
Author(s):  
A. Razzak ◽  
K.R. Roy ◽  
U. Sadia ◽  
H. Mominul ◽  
T. Suvro ◽  
...  

Profile of physicochemical and antioxidant activity of dried Moringa leaves from Bangladesh are presented. Moringa is beneficial for health because it has a lot of nutritional and medicinal values. The leaves were collected and washed with distilled water at different temperatures in an oven dryer, and then the fine powder is taken as a sample by grinding and sieving method. This research was done to compare the changes in physicochemical and antioxidant elements at different temperatures (60°C, 70°C and 80°C) and to find the right temperature at which the nutrient loss will be the lowest. This study showed that as the drying temperature changed, so did the nutrient component of Moringa leaves. Physicochemical parameters (moisture, ash, protein, carbohydrate, fat, color) and antioxidant activity (Total phenol content, DPPH free radical scavenging activity, vitamin C, and ß-carotene) were extracted using a variety of methods. The protein content, carbohydrate content was estimated by the Kjeldahl and phenol sulfuric acid method respectively. Total phenol content (38.30 mg/100g), DPPH (77.79%), and ßcarotene (22.71mg/100g) were measured by the spectrophotometric method. And the colorimeter instrument is used for determining the optical properties. It can be seen that the moisture, ash, protein, carbohydrate, Total phenol content, Vitamin C, DPPH free radical scavenging activity, ß-carotene contents decrease significantly with increasing drying temperature, whereas fat content increases. At 60°C drying temperature the nutrient loss was lowest compared to 70°C and 80°C drying temperature, so it can be concluded that 60°C is the most suitable temperature for drying Moringa leaves.


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