scholarly journals Palmitic acid increases HCK gene and protein expression levels in vascular smooth muscle cells

2021 ◽  
Author(s):  
Ghasem Ghasempour ◽  
Fahimeh Zamani-Garmsiri ◽  
Asghar Mohammadi ◽  
Mohammad Najafi
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Protein Expression ◽  
Smooth Muscle Cells ◽  
Palmitic Acid ◽  
Vascular Smooth Muscle Cells ◽  
Saturated Fatty Acids ◽  
Muscle Cells ◽  
Expression Levels ◽  
Gene And Protein Expression

Abstract Background and Aims: Saturated fatty acids are known to involve in atherosclerosis through different biologic pathways. The aim of this study was to investigate the effects of palmitic acid (PA) on the tyrosine-protein kinase (HCK) gene and protein expression levels in vascular smooth muscle cells (VSMCs). Methods: The human Vascular Smooth Muscle Cells (VSMC) were treated with palmitic acid (0.5 mM, 24 hours) based on the cellular viability studies. The HCK gene and protein expression levels were measured by real-time qRT-PCR and western blotting techniques, respectively. Oil Red O staining method was used to determine the intracellular lipid values.Results: The HCK gene expression level was increased significantly in the PA-treated VSMCs (p=0.02). The total and phosphorylated HCK (p-HCK) protein expression levels increased in VSMCs. There was a significant increase in p-HCK value (P=0.001). Conclusion: The results showed that the palmitic acid increases intracellular p-HCK value so that it may affect the HCK-mediated pathways in VSMCs.

Using gene-network landscape to dissect genotype effects of TCF7L2 genetic variant on diabetes and cardiovascular risk

2012 ◽  
Vol 44 (19) ◽  
pp. 903-914 ◽  
Author(s):  
Andre R. Vaquero ◽  
Noely E. Ferreira ◽  
Samantha V. Omae ◽  
Mariliza V. Rodrigues ◽  
Samantha K. Teixeira ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Gene Interaction ◽  
Muscle Cells ◽  
Correlation Pattern ◽  
Expression Levels ◽  
Expression Correlation ◽  
Splice Forms

The single nucleotide polymorphism (SNP) within the TCF7L2 gene, rs7903146, is, to date, the most significant genetic marker associated with Type 2 diabetes mellitus (T2DM) risk. Nonetheless, its functional role in disease pathology is poorly understood. The aim of the present study was to investigate, in vascular smooth muscle cells from 92 patients undergoing aortocoronary bypass surgery, the contribution of this SNP in T2DM using expression levels and expression correlation comparison approaches, which were visually represented as gene interaction networks. Initially, the expression levels of 41 genes (seven TCF7L2 splice forms and 40 other T2DM relevant genes) were compared between rs7903146 wild-type ( CC) and T2DM-risk ( CT + TT) genotype groups. Next, we compared the expression correlation patterns of these 41 genes between groups to observe if the relationships between genes were different. Five TCF7L2 splice forms and nine genes showed significant expression differences between groups. RXRα gene was pinpointed as showing the most different expression correlation pattern with other genes. Therefore, T2DM risk alleles appear to be influencing TCF7L2 splice form's expression in vascular smooth muscle cells, and RXRα gene is pointed out as a treatment target candidate for risk reduction in individuals with high risk of developing T2DM, especially individuals harboring TCF7L2 risk genotypes.

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