scholarly journals Biomolecular Characterization of Bacillus tequilensis A1C1 Isolated from Soil and Chromatographic Analysis of D-serine in its Cellular Fraction

2021 ◽  
Author(s):  
Shikha Kapil ◽  
Monika Bhattu ◽  
Tarun Kumar ◽  
Vipasha Sharma
Keyword(s):  
Extracellular Enzymes ◽  
Growth Parameters ◽  
Maximum Growth ◽  
Standard Curve ◽  
Intracellular Fraction ◽  
Cellular Fraction ◽  
Negative Results ◽  
Lactose Fermentation ◽  
Bacillus Tequilensis ◽  
Soil Site

Abstract The bacteria isolated from the pomace dumping soil site (bacteria id A1C1) showed maximum growth (O.D600 = 1.97 ± 0.4 X 109 cells/ml) within 48h in the minimal salt media supplemented with L-serine. The isolated strain was identified as ‘Bacillus tequilensis’ through 16sRNA sequencing. The strain was quantified for D-serine content by using RP-HPLC. The D-serine concentration was calculated as 0.919 ± 0.02 nM in the bacterial cellular fraction by using a standard curve plot and linear curve equation. Further, recovery % was also calculated for the spiked samples which vary from 85–90%. The study’s peculiarity reflects the fact that the isolated strain was explored for the first time to detect the presence of serine enantiomers. The biochemical features also showed 70% similarity to the standard strain Bacillus tequilensis 10bT. The optimum growth parameters were recorded as 37℃±0.5, 150 ± 0.5 RPM, and 7 ± 0.5pH. The strain was Gram-positive and synthesized endospores. Morphological results showed its rod shape, large, irregular, and off-white-coloured colonies. A1C1 was also tested for the production of secondary metabolites and enzymes. A1C1 showed positive results for indole production, lactose fermentation, protease, and gelatinase whereas, negative results for catalase, MR-VP, citrate utilization, cellulase, amylase, and pectinase. Further, the strain was assayed for PGPR attributes and showed a negative phosphate solubilization index and IAA production. The antibacterial assay showed 5% and 2% efficacy of the extracellular fraction against MTCC 40 and MTCC 11949 respectively. These results demonstrate that Bacillus tequilensis A1C1 has antibacterial activity, the potential to secrete extracellular enzymes, and D-serine content in the intracellular fraction of the cultivated cells.

scholarly journals Biomolecular Characterization of Bacillus tequilensis A1C1 Isolated from Soil and Chromatographic Analysis of D-serine in its Cellular Fraction

2021 ◽  
Author(s):  
Shikha Kapil ◽  
Monika Bhattu ◽  
Tarun Kumar ◽  
Vipasha Sharma
Keyword(s):  
Extracellular Enzymes ◽  
Growth Parameters ◽  
Maximum Growth ◽  
Standard Curve ◽  
Intracellular Fraction ◽  
Cellular Fraction ◽  
Negative Results ◽  
Lactose Fermentation ◽  
Bacillus Tequilensis ◽  
Soil Site

Abstract The bacteria isolated from the pomace dumping soil site (bacteria id A1C1) showed maximum growth (O.D600 = 1.97±0.4 X 109 cells/ml) within 48h in the minimal salt media supplemented with L-serine. The isolated strain was identified as ‘Bacillus tequilensis’ through 16sRNA sequencing. The strain was quantified for D-serine content by using RP-HPLC. The D-serine concentration was calculated as 0.919±0.02 nM in the bacterial cellular fraction by using a standard curve plot and linear curve equation. Further, recovery % was also calculated for the spiked samples which vary from 85-90%. The study’s peculiarity reflects the fact that the isolated strain was explored for the first time to detect the presence of serine enantiomers. The biochemical features also showed 70% similarity to the standard strain Bacillus tequilensis 10bT. The optimum growth parameters were recorded as 37℃±0.5, 150±0.5 RPM, and 7±0.5pH. The strain was Gram-positive and synthesized endospores. Morphological results showed its rod shape, large, irregular, and off-white-coloured colonies. A1C1 was also tested for the production of secondary metabolites and enzymes. A1C1 showed positive results for indole production, lactose fermentation, protease, and gelatinase whereas, negative results for catalase, MR-VP, citrate utilization, cellulase, amylase, and pectinase. Further, the strain was assayed for PGPR attributes and showed a negative phosphate solubilization index and IAA production. The antibacterial assay showed 5% and 2% efficacy of the extracellular fraction against MTCC 40 and MTCC 11949 respectively. These results demonstrate that Bacillus tequilensis A1C1 has antibacterial activity, the potential to secrete extracellular enzymes, and D-serine content in the intracellular fraction of the cultivated cells.

scholarly journals Biomolecular Characterization of Bacillus tequilensis A1C1 Isolated from Soil and Chromatographic Analysis of D-serine in its Cellular Fraction

2021 ◽  
Author(s):  
Shikha Kapil ◽  
Monika Bhattu ◽  
Tarun Kumar ◽  
Vipasha Sharma
Keyword(s):  
Extracellular Enzymes ◽  
Growth Parameters ◽  
Maximum Growth ◽  
Standard Curve ◽  
Intracellular Fraction ◽  
Cellular Fraction ◽  
Negative Results ◽  
Lactose Fermentation ◽  
Bacillus Tequilensis ◽  
Soil Site

Abstract The bacteria isolated from the pomace dumping soil site (bacteria id A1C1) showed maximum growth (O.D600 = 1.97±0.4 X 109 cells/ml) within 48h in the minimal salt media supplemented with L-serine. The isolated strain was identified as ‘Bacillus tequilensis’ through 16sRNA sequencing. The strain was quantified for D-serine content by using RP-HPLC. The D-serine concentration was calculated as 0.919±0.02 nM in the bacterial cellular fraction by using a standard curve plot and linear curve equation. Further, recovery % was also calculated for the spiked samples which vary from 85-90%. The study’s peculiarity reflects the fact that the isolated strain was explored for the first time to detect the presence of serine enantiomers. The biochemical features also showed 70% similarity to the standard strain Bacillus tequilensis 10bT. The optimum growth parameters were recorded as 37℃±0.5, 150±0.5 RPM, and 7±0.5pH. The strain was Gram-positive and synthesized endospores. Morphological results showed its rod shape, large, irregular, and off-white-coloured colonies. A1C1 was also tested for the production of secondary metabolites and enzymes. A1C1 showed positive results for indole production, lactose fermentation, protease, and gelatinase whereas, negative results for catalase, MR-VP, citrate utilization, cellulase, amylase, and pectinase. Further, the strain was assayed for PGPR attributes and showed a negative phosphate solubilization index and IAA production. The antibacterial assay showed 5% and 2% efficacy of the extracellular fraction against MTCC 40 and MTCC 11949 respectively. These results demonstrate that Bacillus tequilensis A1C1 has antibacterial activity, the potential to secrete extracellular enzymes, and D-serine content in the intracellular fraction of the cultivated cells.

scholarly journals Biomolecular Characterization of Bacillus tequilensis A1C1 Isolated from Soil and Chromatographic Analysis of D-serine in its Cellular Fraction

2021 ◽  
Author(s):  
Shikha Kapil ◽  
Monika Bhattu ◽  
Tarun Kumar ◽  
Vipasha Sharma
Keyword(s):  
Extracellular Enzymes ◽  
Growth Parameters ◽  
Maximum Growth ◽  
Bacterial Cells ◽  
Standard Curve ◽  
Intracellular Fraction ◽  
Cellular Fraction ◽  
Lactose Fermentation ◽  
Bacillus Tequilensis ◽  
Soil Site

Abstract The current work was carried out to investigate serine enantiomers in bacterial cells. The bacteria isolated from the pomace dumping soil site (bacteria id A1C1) showed maximum growth (O.D600 = 1.97±0.4 X 109cells/ml) within 48h in the minimal salt media supplemented with L-serine. The isolated strain was identified as ‘Bacillus tequilensis’ through 16sRNA sequencing. The study’s peculiarity reflects the fact that the isolated strain was explored for the first time to detect the presence of serine enantiomers. The strain was quantified for D-serine content by using RP-HPLC. The D-serine concentration was calculated as 0.919±0.02 nM in the bacterial cellular fraction by using a standard curve plot and linear curve equation. Further, recovery % was also calculated for the spiked samples which vary from 85-90%. The optimum growth parameters were recorded as 37℃±0.5, 150±0.5 RPM, and 7±0.5pH. The strain was Gram-positive, rod shape, large, irregular, off-white-coloured, and synthesized endospores. A1C1 showed positive results (within 14±2h of incubation) for indole production, lactose fermentation, and protease (0.9 mm, clear zone). The antibacterial assay showed 5% and 2% efficacy of the extracellular fraction against MTCC 40 and MTCC 11949 respectively within 12h of incubation. These results demonstrate that Bacillus tequilensis A1C1 has antibacterial activity, the potential to secrete extracellular enzymes, and D-serine content in the intracellular fraction of the cultivated cells. Given results demonstrate the industrial significance and implication of the isolated strain for the synthesis of commercially valuable products.

scholarly journals Isolation and Characterization of a Bacterial Strain Enterobacter cloacae (Accession No. KX438060.1) Capable of Degrading DDTs Under Aerobic Conditions and Its Use in Bioremediation of Contaminated Soil

2021 ◽  
Vol 14 ◽  
pp. 117863612110242
Author(s):  
Sonal Suman ◽  
Tanuja
Keyword(s):  
Contaminated Soil ◽  
Growth Parameters ◽  
Chemical Properties ◽  
Enterobacter Cloacae ◽  
Maximum Growth ◽  
Bacterial Degradation ◽  
Biochemical Tests ◽  
Isolation And Characterization ◽  
16S Rna ◽  
Degrading Bacteria

DDT is one of the most persistent pesticides among all the different types of organo-chlorine pesticides used. Among all the degradation methods, bacterial degradation of DDT is most effective. The present study was conducted to isolate different bacteria present in waste samples which have the ability to degrade DDT present in the soil in the minimum possible period of time and to observe the effect of different physical and chemical properties of the soil samples. Many pesticide degrading bacteria were isolated and identified through cultural, biochemical tests and further identified by 16S RNA sequencing method. The most potent strain DDT 1 growth in mineral salt medium supplemented with DDT as the only source of carbon (5-100 PPM) and was monitored at an optical density of 600 nm. The growth parameters at different physio-chemical conditions were further optimized. The result showed that Enterobacter cloacae had maximum growth in 15 days. FTIR analysis of the residual DDT after 15 days incubation showed that Enterobacter cloacae was able to degrade pesticide into its further metabolites of DDD, DDE, DDNU and other components can be used for biodegradation of DDT present in contaminated soil and water ecosystems.

scholarly journals Efficacy of Experimental Mouth Rinses on Caries-Related Biofilms in vitro

2021 ◽  
Vol 2 ◽  
Author(s):  
Josiana Steiger ◽  
Olivier Braissant ◽  
Tuomas Waltimo ◽  
Monika Astasov-Frauenhoffer
Keyword(s):  
Growth Rate ◽  
Antimicrobial Agents ◽  
Growth Parameters ◽  
Screening Method ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Screening Process ◽  
Mouth Rinses ◽  
Amine Fluoride

This study assessed the efficacy of tin and Polyethylenglycol (PEG-3) tallow aminopropylamine in different concentrations on Streptococcus mutans (S. mutans) biofilms to establish a new screening process for different antimicrobial agents and to gain more information on the antibacterial effects of these agents on cariogenic biofilms. Isothermal microcalorimetry (IMC) was used to determine differences in two growth parameters: lag time and growth rate; additionally, reduction in active biofilms was calculated. Experimental mouth rinses with 400 and 800 ppm tin derived from stannous fluoride (SnF2) revealed results (43.4 and 49.9% active biofilm reduction, respectively) similar to meridol mouth rinse (400 ppm tin combined with 1,567 ppm PEG-3 tallow aminopropylamine; 55.3% active biofilm reduction) (p > 0.05), while no growth of S. mutans biofilms was detected during 72 h for samples treated with an experimental rinse containing 1,600 ppm tin (100% active biofilm reduction). Only the highest concentration (12,536 ppm) of rinses containing PEG-3 tallow aminopropylamine derived from amine fluoride (AmF) revealed comparable results to meridol (57.5% reduction in active biofilm). Lower concentrations of PEG-3 tallow aminopropylamine showed reductions of 16.9% for 3,134 ppm and 33.5% for 6,268 ppm. Maximum growth rate was significantly lower for all the samples containing SnF2 than for the samples containing control biofilms (p < 0.05); no differences were found between the control and all the PEG-3 tallow aminopropylamine (p > 0.05). The growth parameters showed high reproducibility rates within the treated groups of biofilms and for the controls; thus, the screening method provided reliable results.

scholarly journals Azolla as an Alternative of Mineral Nitrogen for Chamomile Plant (Matricaria chamomilla L.) Fertilization in Sandy Soil

2020 ◽  
pp. 1-13
Author(s):  
Kawthar, A. E. Rabie ◽  
M. H. El-Sherif ◽  
R. M. El-Shahat ◽  
Fatma, S. I. Ali
Keyword(s):  
Antioxidant Activity ◽  
Sandy Soil ◽  
Growth Parameters ◽  
Chemical Constituents ◽  
Foliar Spray ◽  
Maximum Growth ◽  
Volatile Oil ◽  
Mineral Nitrogen ◽  
Total Phenolic ◽  
Nitrate And Nitrite

Two pot experiments were carried out during the two successive seasons of 2010/2011 and 2011/ 2012 to apply mineral nitrogen and/or Azolla at different methods of addition with a reduction of mineral nitrogen fertilizer and its effect on vegetative growth, the yield of inflorescences, total phenolic concentration, antioxidant activity, nitrate and nitrite concentrations and the volatile oil concentrations and composition of chamomile inflorescences in sandy soil. Data indicated that dry Azolla (DA) treatments increased significantly plant height, number of branches/plant, shoot fresh and dry weights and number of inflorescences/plant against the rest treatments. It is clear that DA singly induced maximum growth parameters in both seasons and the reverse was true with fresh Azolla (FA) alone. It is obvious that DA achieved the highest total phenolic concentrations, antioxidant activity and volatile oil (VO) concentrations, and the opposite response was true for nitrate & nitrite concentrations. Addition of 50% ammonium nitrate to 50% FA or 50% Azolla extract as foliar (spray Azolla) SA have positive effect on chemical constituents except nitrate and nitrite concentrations. It is evident that the highest chamazulene and α ̶ bisabolol oxide ̶ B were achieved with DA and the reverse was true for α ̶ bisabolol oxide ̶ A. Dry Azolla treatment alone was the most efficient one in increasing the yield production with increment in active substances of chamomile plant in sandy soil.

scholarly journals Optimum Potassium Concentrations in Recirculating Subirrigation for Selected Greenhouse Crops

HortScience ◽  
2004 ◽  
Vol 39 (6) ◽  
pp. 1441-1444 ◽  
Author(s):  
Trisha Blessington Haley ◽  
David Wm. Reed
Keyword(s):  
Growth Parameters ◽  
New Guinea ◽  
Middle Layer ◽  
Maximum Growth ◽  
Bottom Layer ◽  
Growing Medium ◽  
K Fertilizer ◽  
K Concentration ◽  
New Guinea Impatiens ◽  
Recommended Level

Two experiments were conducted to investigate the effect of K fertilizer rates on growth of New Guinea impatiens (Impatiens Hawkeri Bull.), vinca (Catharanthus roseus (L.) G. Don) and petunia (Petunia ×hybrida Hort. Vilm.-Andr.) in a recirculating subirrigation system. Based on a variety of growth parameters, a broad range of K concentrations allowed maximum growth, notably 1 to 6 mM for New Guinea impatiens `Ovation Salmon Pink Swirl', 2 mm for New Guinea impatiens `Cameo' and `Illusion', 2 to 8 mm for vinca `Pacifica Apricot', and 2 to 16 mm for petunia `Trailing Wave Misty Lilac'. Thus, the lowest concentration that allowed maximum growth was 1 to 2 mm K. A third experiment compared the optimum K concentration and K balance of vinca grown with recirculating subirrigation versus top-watering. Based on a variety of growth parameters of vinca `Pacifica Red', the lowest concentration that allowed maximum growth was 2 mm K with recirculating subirrigation and 4 mm K with top-watering. The K balance demonstrated that subirrigated plants were twice as efficient in K use compared to the top-watered plants. Leachate loss was the major contributor to inefficiency in top-watered plants. Electrical conductivity (EC) of the growing medium remained below the recommended level of 1.2 dS·m-1 in both irrigation methods at K concentrations of 16 mm and below in the bottom layer and 8 mm and below in the middle layer. In the top layer of the growing medium, EC was above the recommended level at all K concentrations tested in subirrigation at all concentrations, and in top-watering at 16 mm and above.

scholarly journals (126) Response of Six Begonia Species to Different Substrate EC

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1085D-1085
Author(s):  
Ka Yeon Jeong ◽  
Claudio Pasian ◽  
David Tay
Keyword(s):  
Growth Parameters ◽  
Block Design ◽  
Growth Parameter ◽  
Dry Weight ◽  
Maximum Growth ◽  
Stem Length ◽  
Stem Cuttings ◽  
Limited Information ◽  
Spad Value ◽  
Parameter Values

There is limited information on optimal substrate EC level for begonia species (noncultivated hybrids). The objective of this study was to evaluate the response of six species to different substrate EC in a greenhouse. Begonia albopicta, B. cucullata var. cucullata, B. echinosepala var. elongatifolia, B. holtonis, B. fuchsioides (red) and B. fuchsioides (pink) plants were propagated by stem cuttings, and transplanted into plastic pots using a soilless mix. Five concentrations (20, 80, 200, 400, and 600 mg·L-1 N) of 17–5–17 fertilizer were applied as irrigation water to derive the five substrate EC levels. This experiment was a factorial randomized complete-block design. Substrate EC was measured weekly using the PourThru method and averaged for each treatment of each species. Inflorescence number, the longest stem length, SPAD readings, leaf area, and dry weight of each plant were measured as growth parameters. There were significant responses to substrate EC level and species on begonia growth parameters. The highest growth parameters of B. albopicta and B. cucullata were obtained at EC 5.7 and 6.6 mS·cm-1, respectively. The maximum growth of B. echinosepala and B. holtonis was observed at 2.6 and 3.0 mS·cm-1, respectively. B. fuchsioides, grown at 1.2 mS·cm-1, had the best growth parameter values. As EC level increased, SPAD value for B. fuchsioides (pink) and B. holtonis also increased. The highest SPAD reading was observed at EC 3.7 mS·cm-1 for B. albopicta, EC 6.6 mS·cm-1 for B. cucullata, EC 2.6 mS·cm-1 for B. echinosepala, and EC 4.1 mS·cm-1 for B. fuchsioides (red). Plant mortality of several begonia species was observed when grown at EC value above 6.4 or below 4.4 mS·cm-1.

scholarly journals Sex-specific growth of nestlings of the Whiskered Tern Chlidonias hybrida, a species with sexual size dimorphism and female brood desertion

2021 ◽  
Author(s):  
Agata Banach ◽  
Grzegorz Neubauer ◽  
Adam Flis ◽  
Mateusz Ledwoń
Keyword(s):  
Growth Rate ◽  
Body Mass ◽  
Sexual Size Dimorphism ◽  
Growth Parameters ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Head Length ◽  
Whiskered Tern ◽  
Total Head ◽  
Chlidonias Hybrida

AbstractThe growth of a nestling during the first few weeks after hatching is crucial for its further life and is sensitive to the conditions experienced during this period. Among species exhibiting Sexual Size Dimorphism (SSD), one of the most important factors influencing growth parameters is the sex of the hatchlings. In this study, we tested whether sex and other factors (hatching date, egg volume, brood size and sex ratio in the brood) were related to sex on growth parameters in Whiskered Tern (Chlidonias hybrida) chicks. In this species, males are 3%–10% larger than females when adult. At hatching, only the total head length was greater in males than females, but values close to fledging were greater for most of the measured parameters (3%—tarsus, 4%—total head length, 13%—body mass). Moreover, the maximum body mass growth rate was higher in males but females entered the rapid growth phase somewhat earlier and achieved the final total head length sooner. Nestling growth was also significantly correlated with brood size and hatching date, though mostly negatively (e.g. lower tarsus and total head length asymptote, lower maximum growth rate and growth rate constant of total head length in bigger and later clutches). The occurrence of SSD during the nestling period and higher maximum growth rate of body mass in males indicates that the costs of raising sons may be higher than of raising daughters. However, we did not find evidence of either sex-biased nestling mortality or skewed sex ratio.

scholarly journals A description of the growth of sheep and its genetic analysis

2002 ◽  
Vol 74 (1) ◽  
pp. 51-62 ◽  
Author(s):  
R.M. Lewis ◽  
G.C. Emmans ◽  
W.S. Dingwall ◽  
G. Simm
Keyword(s):  
Growth Rate ◽  
Growth Parameters ◽  
Live Weight ◽  
Great Majority ◽  
Maximum Growth ◽  
Rate Parameter ◽  
Daily Growth ◽  
Lean Growth ◽  
Lumped Parameter ◽  
Mature Size

AbstractThe Gompertz is one of a family of growth functions that, when the environment (e.g. food, housing) is non-limiting, provides a useful description of growth as a comparatively simple, single equation. It has three parameters of which the important ones are mature size, A, and the rate parameter, B. Estimates of A and B, however, are highly correlated and defining their separate values for individual animals is problematic. This problem was explored using five methods for estimating the parameters, or transformations of them, to describe the growth of two genotypes of Suffolk sheep kept under non-limiting conditions. One genotype was under selection for high lean growth rate and the other was its control. Live weights that were collected at least fortnightly from near birth to 150 days of age over a 9-year period on 1934 lambs were used. The Gompertz form adequately described the growth of the great majority of the lambs evaluated. When considering A and B as a lumped parameter, Z = A·B, and fitting Z, B and an initial condition (a transformed birth weight) as the parameters, the problems in estimation were substantially overcome as shown by a low correlation of Z with estimates of B both within and across animals. Usefully Z has a biological interpretation in that Z/e is the maximum daily growth rate. Since the Gompertz form adequately described growth in these sheep, the extent of genetic co-variation for the growth parameters values (A, B, Z) was estimated to determine if they were amenable to selection. A weighted univariate animal model was fitted. Mature size, A, and the rate parameter, B, were moderately heritable (0·37 (s.e. 0·04) and 0·38 (s.e. 0·05), respectively) as was live weight at 150 days of age (0·31 (s.e. 0·06)). However there was a substantial negative genetic relationship between A and B (–0·48). Z was highly heritable (0·72 (s.e.0·05)). After 9 years of selection, the genotype selected for high lean growth rate was heavier (P < 0·001) at 150 days of age (5·2 kg) and at maturity (6·6 kg), with a maximum growth rate (Z/e) that was 1·12 times that of the control. Our lumped parameter Z, in effect a rate parameter scaled for mature size, avoided problems in estimating A and B and, in so doing, offers a general and robust description of lamb growth amenable to selection.

Sign in / Sign up

Export Citation Format

Share Document