scholarly journals Delivery of dCas9 CRISPR System Into the Hard Transfection Cells by Magnetofection Approach

2021 ◽  
Author(s):  
Mahdi Mohammadi Ghanbarlou ◽  
Shahriyar Abdoli ◽  
Hadi Bamehr ◽  
Leila Qazizadeh ◽  
Hamed Omid ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Genome Engineering ◽  
Colloidal Stability ◽  
Transfection Efficiency ◽  
Superparamagnetic Iron Oxide ◽  
Eukaryotic Cells ◽  
Dna Encoding ◽  
Hek 293 ◽  
Co Precipitation ◽  
Delivery Efficiency

Abstract Background: The CRISPR-Cas9 system, a powerful tool, has revolutionized genome engineering in eukaryotic cells and living organisms. However, this approach poses unique concerns and limitations when used by conventional transfection methods, including limited packaging size and low delivery efficiency. Here, we aim at assessing the transfection efficiency of DNA encoding for the CRISPR-Cas9 system by PEI coated Magnetic NanoParticle (MNPs) to improve the delivery of CRISPR/Cas9 constructs into eukaryotic cells. Results: Superparamagnetic iron oxide nanoparticles (SPIONs) coated with polyethylenimine (PEI) and then complexed with pCXLE-dCas9VPH-T2A-GFP-shP53 plasmid DNA. We used HEK-293 (human embryonic kidney) and Human foreskin fibroblasts (HFF) cells to express GFP after transfection to evaluate delivery efficiency with MNPs and Lipofection methods. PEI-coated nanoparticles with magnetic iron oxide core were synthesized by co-precipitation technique resulting in an average size of ~ 20 nm in diameter. Characterization of Magnetic Nano Particle (MNPs) revealed that particles have narrow size distribution sufficient colloidal stability. The result showed that the magnetofection method with an efficiency around 85.7% for HEK-293 and 28.2% for HFF. Also, transfection efficiency by lipofection method was 83.2% and 7.89% for HEK-293 and HFF respectively. Conclusion: The magnetofection was revealed to be more efficient than classic Lipofectamine transfection as measured by GFP expression. We show that PEI-MNPs enable effective delivery and improved safety of plasmids encoding CRISPR/Cas9 into eukaryote cells.

Superparamagnetic Iron Oxide Nanoarticles for Biomedical Applications: a focus on PVA as a coating

2003 ◽  
Vol 789 ◽  
Author(s):  
M. Chastellain ◽  
A. Petri ◽  
H. Hofmann
Keyword(s):  
Iron Oxide ◽  
Biomedical Applications ◽  
Large Scale ◽  
Colloidal Stability ◽  
Superparamagnetic Iron Oxide ◽  
Narrow Size Distribution ◽  
Water Based ◽  
Co Precipitation

ABSTRACTNanoscaled particles showing a superparamagnetic behavior have been intensively studied these past years for biomedical applications and water-based ferrofluids turned out to be promising candidates for various in vivo as well as in vitro applications. Nevertheless, the lack of well-defined particles remains an important problem. One of the major challenges is still the large-scale synthesis of particles with a narrow size distribution. In this work iron oxide nanoparticles are obtained by classical co-precipitation in a water-based medium and are subsequently coated with polyvinyl alcohol. The thus obtained ferrofluids are studied and a focus is made on their colloidal stability.

Sonochemical Synthesis of Silica Coated Super Paramagnetic Iron Oxide Nanoparticles

2013 ◽  
Vol 756 ◽  
pp. 74-79 ◽  
Author(s):  
Bashiru Kayode Sodipo ◽  
Azlan Abdul Aziz
Keyword(s):  
Iron Oxide ◽  
Silica Nanoparticles ◽  
Iron Oxide Nanoparticles ◽  
Ph Value ◽  
Superparamagnetic Iron Oxide ◽  
Silica Nanoparticle ◽  
Precipitation Method ◽  
Oxide Nanoparticles ◽  
Sonochemical Method ◽  
Co Precipitation

Superparamagnetic iron oxide nanoparticles (SPION) of sizes 5 to10 nm were synthesized by the co-precipitation method. They are coated with silica nanoparticles using sonication method. The SPION was produced under the optimum pH of 10, peptized in acidic medium and redispersed in water. The silica nanoparticles were produced through the Stöbermethod. Sonochemical coating of silica nanoparticle on the SPION was successfulat a pH value lower than 5. Otherwise, at higher pH value (but lower than point zero charge (PZC)), the SPION were found to be unstable. Fast hydrolysis of triethoxyvinylsilane(TEVS) shows that silica forms its own particles without coating onto the surfaces of the SPION. Under optimized experimental condition, sonochemical method of coating silica nanoparticles onto the SPION can be considered as an alternative for effective and prompt method that rely mainly on pH of the suspension.

COLLOIDAL STABILITY AND MONODISPERSIBLE MAGNETIC IRON OXIDE NANOPARTICLES IN BIOTECHNOLOGY APPLICATION

2013 ◽  
Vol 12 (06) ◽  
pp. 1330002 ◽  
Author(s):  
K. SHAMILI ◽  
E. M. RAJESH ◽  
R. RAJENDRAN ◽  
S. R. MADHAN SHANKAR ◽  
M. ELANGO ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Biomedical Applications ◽  
Colloidal Stability ◽  
Oxide Nanoparticles ◽  
Magnetic Iron Oxide Nanoparticles ◽  
Magnetic Fluid Hyperthermia ◽  
Magnetic Iron Oxide ◽  
Magnetic Resonance Imaging Mri ◽  
Co Precipitation

Magnetic iron oxide nanoparticles are promising material for various biological applications. In the recent decades, magnetic iron oxide nanoparticles (MNPs) have great attention in biomedical applications such as drug delivery, magnetic resonance imaging (MRI) and magnetic fluid hyperthermia (MFH). This review focuses on the colloidal stability and monodispersity properties of MNPs, which pay more attention toward biomedical applications. The simplest and the most promising method for the synthesis of MNPs is co-precipitation. The biocompatible MNPs are more interested in MRI application. This review also apportions synthesis, characterization and applications of MNP in biological and biomedical as theranostics and imaging.

Magnetic behavior of coated superparamagnetic iron oxide nanoparticles in ferrofluids

2001 ◽  
Vol 676 ◽  
Author(s):  
W. Voit ◽  
D. K. Kim ◽  
W. Zapka ◽  
M. Muhammed ◽  
K. V. Rao
Keyword(s):  
Particle Size ◽  
Iron Oxide ◽  
Superparamagnetic Iron Oxide ◽  
Magnetic Behavior ◽  
Blocking Temperature ◽  
Iron Oxide Particles ◽  
Physical Particle ◽  
Superparamagnetic Iron Oxide Particles ◽  
Oxide Particles ◽  
Co Precipitation

ABSTRACTWe present a study on the magnetic behavior of nanosized iron oxide particles coated with different surfactants (sodium oleate, PVA and starch) in a ferrofluid. The effect of the coating material, and different particle concentrations in the ferrofluid have been magnetically investigated to determine the effective magnetic particle size and possible interaction. The superparamagnetic iron oxide particles, synthesized by a controlled co-precipitation technique, are found to contain magnetite (Fe3O4) as a main phase with a narrow physical particle size distribution between 6 and 8 nm. The mean effective magnetic size of the particles in different ferrofluid systems are estimated to be around 4-5 nm which is smaller than the physical particle size. On a 10% dilution in the starch coated ferrofluid we observe a decrease in the blocking temperature.

Preparation, characterization and application of superparamagnetic iron oxide nanoparticles modified with natural polymers for removal of 60Co-radionuclides from aqueous solution

2017 ◽  
Vol 105 (2) ◽  
Author(s):  
Gehan E. Sharaf El-Deen ◽  
Neama G. Imam ◽  
Refaat R. Ayoub
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Precipitation Method ◽  
Oxide Nanoparticles ◽  
Natural Polymers ◽  
Ft Ir ◽  
Co Precipitation ◽  
Ft Ir Spectroscopy

AbstractSuperparamagnetic iron oxide nanoparticles (IO-MNPs) coated with natural polymers, starch (IO-S MNPs) and dextrin (IO-D MNPs), were synthesized by modified co-precipitation method. IO and hybrid-IO-MNPs were characterized by XRD, SEM, HRTEM, FT-IR spectroscopy, vibrating sample magnetometer (VSM) and zeta potential (ZP). IO-S MNPs and IO-D MNPs have IO core-shell structure with core of 10.8 nm and 13.8 nm and shell of 7.5 nm and 5.9 nm, respectively. The efficiency of the hybrid IO-MNPs for sorption of

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