Superparamagnetic Iron Oxide Nanoarticles for Biomedical Applications: a focus on PVA as a coating

2003 ◽  
Vol 789 ◽  
Author(s):  
M. Chastellain ◽  
A. Petri ◽  
H. Hofmann

ABSTRACTNanoscaled particles showing a superparamagnetic behavior have been intensively studied these past years for biomedical applications and water-based ferrofluids turned out to be promising candidates for various in vivo as well as in vitro applications. Nevertheless, the lack of well-defined particles remains an important problem. One of the major challenges is still the large-scale synthesis of particles with a narrow size distribution. In this work iron oxide nanoparticles are obtained by classical co-precipitation in a water-based medium and are subsequently coated with polyvinyl alcohol. The thus obtained ferrofluids are studied and a focus is made on their colloidal stability.

2021 ◽  
Vol 17 (2) ◽  
pp. 205-215
Author(s):  
Zhenbo Sun ◽  
Mingfang Luo ◽  
Jia Li ◽  
Ailing Wang ◽  
Xucheng Sun ◽  
...  

Imaging-guided cancer theranostic is a promising strategy for cancer diagnostic and therapeutic. Photodynamic therapy (PDT), as an approved treatment modality, is limited by the poor solubility and dispersion of photosensitizers (PS) in biological fluids. Herein, it is demonstrated that superparamagnetic iron oxide (SPIO)-based nanoparticles (SCFs), prepared by conjugated with Chlorin e6 (Ce6) and modified with folic acid (FA) on the surface, can be used as versatile drug delivery vehicles for effective PDT. The nanoparticles are great carriers for photosensitizer Ce6 with an extremely high loading efficiency. In vitro fluorescence imaging and in vivo magnetic resonance imaging (MRI) results indicated that SCFs selectively accumulated in tumor cells. Under near-infrared laser irradiation, SCFs were confirmed to be capable of inducing low cell viability of RM-1 cells In vitro and displaying efficient tumor ablation with negligible side effects in tumor-bearing mice models.


2010 ◽  
Vol 19 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Po-Wah So ◽  
Tammy Kalber ◽  
David Hunt ◽  
Michael Farquharson ◽  
Alia Al-Ebraheem ◽  
...  

Determination of the dynamics of specific cell populations in vivo is essential for the development of cell-based therapies. For cell tracking by magnetic resonance imaging (MRI), cells need to internalize, or be surface labeled with a MRI contrast agent, such as superparamagnetic iron oxide nanoparticles (SPIOs): SPIOs give rise to signal loss by gradient-echo and T2-weighted MRI techniques. In this study, cancer cells were chemically tagged with biotin and then magnetically labeled with anti-biotin SPIOs. No significant detrimental effects on cell viability or death were observed following cell biotinylation. SPIO-labeled cells exhibited signal loss compared to non-SPIO-labeled cells by MRI in vitro. Consistent with the in vitro MRI data, signal attenuation was observed in vivo from SPIO-labeled cells injected into the muscle of the hind legs, or implanted subcutaneously into the flanks of mice, correlating with iron detection by histochemical and X-ray fluorescence (XRF) methods. To further validate this approach, human mesenchymal stem cells (hMSCs) were also employed. Chemical biotinylation and SPIO labeling of hMSCs were confirmed by fluorescence microscopy and flow cytometry. The procedure did not affect proliferation and multipotentiality, or lead to increased cell death. The SPIO-labeled hMSCs were shown to exhibit MRI signal reduction in vitro and was detectable in an in vivo model. In this study, we demonstrate a rapid, robust, and generic methodology that may be a useful and practical adjuvant to existing methods of cell labeling for in vivo monitoring by MRI. Further, we have shown the first application of XRF to provide iron maps to validate MRI data in SPIO-labeled cell tracking studies.


2019 ◽  
Vol 21 ◽  
pp. 102063 ◽  
Author(s):  
Vladimir Mulens-Arias ◽  
José Manuel Rojas ◽  
Laura Sanz-Ortega ◽  
Yadileiny Portilla ◽  
Sonia Pérez-Yagüe ◽  
...  

Author(s):  
Wenzhen Zhu ◽  
Xiang Li ◽  
Zhouping Tang ◽  
Suiqiang Zhu ◽  
Jianpin Qi ◽  
...  

2013 ◽  
Vol 12 (06) ◽  
pp. 1330002 ◽  
Author(s):  
K. SHAMILI ◽  
E. M. RAJESH ◽  
R. RAJENDRAN ◽  
S. R. MADHAN SHANKAR ◽  
M. ELANGO ◽  
...  

Magnetic iron oxide nanoparticles are promising material for various biological applications. In the recent decades, magnetic iron oxide nanoparticles (MNPs) have great attention in biomedical applications such as drug delivery, magnetic resonance imaging (MRI) and magnetic fluid hyperthermia (MFH). This review focuses on the colloidal stability and monodispersity properties of MNPs, which pay more attention toward biomedical applications. The simplest and the most promising method for the synthesis of MNPs is co-precipitation. The biocompatible MNPs are more interested in MRI application. This review also apportions synthesis, characterization and applications of MNP in biological and biomedical as theranostics and imaging.


2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Liang Ma ◽  
Ming-wei Li ◽  
Yu Bai ◽  
Hui-hui Guo ◽  
Sheng-chao Wang ◽  
...  

Tracking transplanted stem cells is necessary to clarify cellular properties and improve transplantation success. In this study, we investigate the effects of fluorescent superparamagnetic iron oxide particles (SPIO) (Molday ION Rhodamine-B™, MIRB) on biological properties of human dental pulp stem cells (hDPSCs) and monitor hDPSCs in vitro and in vivo using magnetic resonance imaging (MRI). Morphological analysis showed that intracellular MIRB particles were distributed in the cytoplasm surrounding the nuclei of hDPSCs. 12.5–100 μg/mL MIRB all resulted in 100% labeling efficiency. MTT showed that 12.5–50 μg/mL MIRB could promote cell proliferation and MIRB over 100 μg/mL exhibited toxic effect on hDPSCs. In vitro MRI showed that 1 × 106cells labeled with various concentrations of MIRB (12.5–100 μg/mL) could be visualized. In vivo MRI showed that transplanted cells could be clearly visualized up to 60 days after transplantation. These results suggest that 12.5–50 μg/mL MIRB is a safe range for labeling hDPSCs. MIRB labeled hDPSCs cell can be visualized by MRI in vitro and in vivo. These data demonstrate that MIRB is a promising candidate for hDPSCs tracking in hDPSCs based dental pulp regeneration therapy.


2008 ◽  
Vol 4 (4) ◽  
pp. 318-329 ◽  
Author(s):  
Chengli Yang ◽  
Antonina Rait ◽  
Kathleen F. Pirollo ◽  
John A. Dagata ◽  
Natalia Farkas ◽  
...  

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