scholarly journals IN VITRO GROWTH RATE OF Kappaphycus alvarezii MICROPROPAGULE AND EMBRYO BY ENRICHMENT MEDIUM WITH SEAWEED EXTRACT

2015 ◽  
Vol 10 (1) ◽  
pp. 13
Author(s):  
Emma Suryati ◽  
Rosmiati Rosmiati ◽  
Andi Parenrengi ◽  
Andi Tenriulo
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Liquid Medium ◽  
Callus Induction ◽  
Kappaphycus Alvarezii ◽  
Seaweed Extract ◽  
In Vitro Growth ◽  
Seaweed Cultivation ◽  
Enrichment Medium

The development of micropropagule and embryo of seaweed depend on nutrient and fertilizer used. Seaweed has been reported contain hormone regulators such as auxine, cytokinine, gibbereline, and various minerals applied in stimulating the growth ocra plant and wheat culture. The objectives of this study were to determine the potential of Kappaphycus alvarezii extract and its optimal concentration in accelerating of Kappaphycus alvarezii micropropagule and embryo growth. Micropropagule and embryo produced through callus induction were planted into PES 1/20 liquid medium supplemented with seaweed extract at the concentrations of 0 (control), 25, 50, 75, and 100 μL in 10 mL of medium. The results showed that medium enrichment with 50 μL of seaweed extract had the highest survival rate and growth of thallus. In addition, this concentration was also resulted in a good performance of K. alvarezii thallus with the lighter color. The advantage of this study for seaweed cultivation in Indonesia, among others, seaweed can be used as fertilizer, especially in the maintenance of seaweed seed, so that cultivation can be better develop.

scholarly journals In Vitro Culture of Seaweed Kappaphycus alvarezii under Different Formulation of Growth Stimulating Substances and Culture Media

2019 ◽  
Vol 7 (1) ◽  
pp. 274
Author(s):  
Mega D. Dalero ◽  
Grevo S. Gerung ◽  
Edwin L.A. Ngangi ◽  
Lawrence J.L. Lumingas ◽  
Markus T. Lasut
Keyword(s):  
Abscisic Acid ◽  
Survival Rate ◽  
In Vitro Culture ◽  
Culture Media ◽  
Kappaphycus Alvarezii ◽  
Seed Stock ◽  
In Vitro Growth ◽  
Parent Plant ◽  
Lower Growth

This study aims at obtaining a sustainably superior seed stock following the characteristics of the parent plant, determining the best formulation of the growth stimulating substance. In general, cytokinin and auxin combination was used, but this study also added with the combination of cytokinin and giberelin and cytokinin and abscisic acid (AA).Parameters measured were bud length, number of buds, and survival rate. Bacterial Vibrio sp test was also done as a cause of the explant mortality. Results showed that the longest bud was recorded in treatment C (S+A 1:2.5) cultured in a jar, 1.343 mm long, 38% of survival, while the highest number of buds was found in treatment B (S+A 1 : 2) 8.86. The shortest bud was recorded in treatment J (S + AA 1:2.5) cultured in a jar, 0.093 mm long, 2.64 buds, 10% of survival, while the explant cultured in the bottle had a length of 0.051 mm long, 1.50 buds, 4% of survival. As conclusion, the best growth stimulating substance was found in the treatment C for the bud length and the survival rate, while the best number of bud was recorded in the treatment B. The best culture tank was topless bottle (aerated). In vitro culture could also use S + G formulation. The explant mortality was caused by Vibrio charchariae. The use of S + AA formulation had lower growth than that of control treatment.Keywords :in vitro, growth stimulating substance, culture media, Kappaphycus alvarezii, Vibrio charchariae ABSTRAKPenelitian ini bertujuan untuk memperoleh benih unggul secara berkelanjutan yang mengikuti karakteristik dari tanaman induk, menentukan formulasi terbaik dari substansi pertumbuhan merangsang. Secara umum, kombinasi sitokinin dan auksin digunakan, tetapi penelitian ini juga menambahkankombinasi sitokinin, giberelin, sitokinin dan asam absisat (AA). Parameter yang diukur adalah panjang tunas, jumlah tunas, dan tingkat kelangsungan hidup. Bakteri Vibrio Uji sp juga dilakukan sebagai penyebab kematian eksplan . Hasil penelitian menunjukkan bahwa tunas terpanjang terdapat pada perlakuan C (S + A 1: 2,5)  kultur dalam toples, 1,343 mm, 38% hidup, sementara jumlah tertinggi tunas ditemukan pada perlakuan B (S + A 1: 2) 8.86 . Jumlah tunas paling sedikit terdapat pada perlakuan J (S + AA 1: 2,5) yang dikultur dalam toples, 0,093 mm, 2,64 tunas, 10% hidup, sedangkan eksplan yang dikultur dalam botol memiliki panjang 0.051 mm, 1. 50 tunas , 4% bertahan hidup. Sebagai kesimpulan, pertumbuhan terbaik merangsang zat ditemukan dalam perlakuan C untuk panjang tunas dan tingkat kelangsungan hidup, sementara jumlah tunas terbanyak ditemukan pada perlakuan B. Penggunaan wadah budaya terbaik adalah topless yang diaerasi. Kultur in vitro juga dapat menggunakan formulasi S + G. Kematian eksplan disebabkan oleh Vibrio charchariae . Penggunaan formulasi S + AA memiliki pertumbuhan yang lebih rendah dari pada pengobatan kontrol .Kata kunci : in vitro, zat perangsang tumbuh, media kultur, Kappaphycus alvarezii, Vibrio charchariae

Growth rate of the carrageenophyte Kappaphycus alvarezii (Rhodophyta, Gigartinales) in vitro

2001 ◽  
Vol 49 (3) ◽  
pp. 155-161 ◽  
Author(s):  
Edison Jose De Paula ◽  
Cintia Erbert ◽  
Ricardo Toledo Lima Pereira
Keyword(s):  
Growth Rate ◽  
Kappaphycus Alvarezii

Effects of salinity on the growth rate, carrageenan yield, and cellular structure of Kappaphycus alvarezii (Rhodophyta, Gigartinales) cultured in vitro

2010 ◽  
Vol 23 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Leila Hayashi ◽  
Gabriel S. M. Faria ◽  
Beatriz G. Nunes ◽  
Carmen S. Zitta ◽  
Lidiane A. Scariot ◽  
...  
Keyword(s):  
Growth Rate ◽  
Cellular Structure ◽  
Kappaphycus Alvarezii ◽  
Carrageenan Yield

scholarly journals MICROPROPAGATION OF MUSA ON A STATIONARY LIQUID MEDIUM

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696g-696
Author(s):  
Joan P. Harris
Keyword(s):  
Liquid Medium ◽  
Mutation Rate ◽  
Multiplication Rate ◽  
In Vitro Growth ◽  
Liquid Media ◽  
Stationary Liquid ◽  
One Year

In vitro growth of banana, Musa sp., using a modified Murashige and Skoog liquid medium was compared with the same formulation solidified by the addition of agar. Plants grown on the stationary liquid weighed more and had a higher multiplication rate. The best volume of liquid and number of plants per vessel were determined. Four hundred plants grown in liquid media were studied for one year after planting in the field. Flowering occurred at 7 months and harvesting 3 months later. The mutation rate observed was below 5%.

scholarly journals Linear Correlation Analysis of Zymoseptoria tritici Aggressiveness with In Vitro Growth Rate

2016 ◽  
Vol 106 (11) ◽  
pp. 1255-1261 ◽  
Author(s):  
Fangfang Zhan ◽  
Yiekun Xie ◽  
Wen Zhu ◽  
Danli Sun ◽  
Bruce A. McDonald ◽  
...  
Keyword(s):  
Growth Rate ◽  
Pathogenic Fungus ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
In Vitro Growth ◽  
Zymoseptoria Tritici ◽  
Average Growth ◽  
Positive Correlation ◽  
Linear Correlation Analysis

Zymoseptoria tritici is a globally distributed plant-pathogenic fungus causing Septoria tritici blotch of wheat. In this study, the in vitro growth rates and aggressiveness of 141 genetically distinct isolates sampled from four wheat fields on three continents were assessed to determine the association of these two ecological parameters. Aggressiveness was assessed on two spring wheat cultivars (‘Toronit’ and ‘Greina’) in a greenhouse using percentages of leaf area covered by lesions and pycnidia. We found a positive correlation between aggressiveness of pathogen strains on the two cultivars, consistent with a quantitative and host-nonspecific interaction in this pathosystem. We also found a positive correlation between aggressiveness and average growth rate at two temperatures, suggesting that in vitro pathogen growth rate may make a significant contribution to pathogen aggressiveness.

25 Inhibition of the in vitro growth rate of L1210 leukemic cells by platinum(II) derivatives

Biochimie ◽  
1978 ◽  
Vol 60 (9) ◽  
pp. 1054-1055 ◽  
Author(s):  
J.L. Butour ◽  
J.P. Macquet ◽  
C. Paoletti
Keyword(s):  
Growth Rate ◽  
Leukemic Cells ◽  
In Vitro Growth

scholarly journals Kultur Embrio Pisang Liar Musa acuminata ssp. sumatrana yang Langka (Embryo Culture of Endangered Wild Banana Musa acuminata ssp. sumatrana)

2019 ◽  
Vol 28 (1) ◽  
pp. 25
Author(s):  
Ika Roostika ◽  
Agus Sutanto ◽  
NFN Edison ◽  
Nurwita Dewi
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Combined Treatment ◽  
Shoot Multiplication ◽  
Musa Acuminata ◽  
In Vitro Conservation ◽  
Plant Materials ◽  
Randomized Design ◽  
Wild Banana

<p><em>Musa acuminata</em> ssp. sumatrana adalah pisang liar yang langka dan perlu dilestarikan. Teknik kultur in vitro dapat diterapkan untuk melestarikannya. Penerapan teknik konservasi secara in vitro memerlukan penguasaan metode regenerasi, termasuk formulasi media tumbuh. Penelitian bertujuan mengetahui pengaruh BA, TDZ, dan PVP terhadap daya hidup dan regenerasi embrio M. acuminata ssp. sumatrana. Rancangan percobaan disusun secara faktorial dalam lingkungan rancangan acak lengkap dengan empat ulangan. Faktor pertama adalah BA (0, 1, 3, dan 5 mg/L), faktor kedua adalah TDZ (0 dan 0,1 mg/L) dan faktor ketiga adalah PVP (100 dan 300 mg/L). Peubah yang diamati adalah persentase daya hidup, persentase daya tumbuh, persentase pembentukan akar, jumlah tunas, jumlah akar, dan jumlah daun yang terbentuk. Hasil penelitian menunjukkan terdapat interaksi yang nyata antara taraf BA, TDZ, dan PVP terhadap seluruh peubah yang diamati. Kombinasi perlakuan BA 5 mg/L dengan TDZ 0,1 mg/L dan PVP 300 mg/L menghasilkan persentase hidup, persentase tumbuh, jumlah tunas, dan jumlah daun yang paling tinggi, berturut-turut 100% daya hidup, 100% daya tumbuh, lima tunas/eksplan, dan 15 daun/tunas. Media tersebut dapat diterapkan untuk perbanyakan in vitro M. acuminata ssp. sumatrana dalam penyediaan materi untuk konservasi in vitro.</p><p><strong>Keywords</strong></p><p><em>Musa acuminata</em> ssp. <em>sumatrana</em>; Pisang liar langka; Regenerasi embrio</p><p><strong>Abstract</strong></p><p><em>Musa acuminata</em> ssp. sumatrana is an endangered wild banana species that should be conserved. The in vitro culture can be applied for conserving wild banana accessions. The establishment of regeneration method, including the use of growth medium, is required in the application of in vitro conservation. The influence of BA, TDZ, and PVP to the survival and growth rate of embryos of M. acuminata ssp. sumatrana will be discussed in this study. The factorial in compeletely randomized design with four replications was used in this study. The first factor was BA (0, 1, 3, and 5 mg/L), the second factor was TDZ (0 and 0.1 mg/L), and the third factor was PVP (100 and 300 mg/L). Variables observed were the percentage of survival rate, the percentage of growth rate, the percentage of root formation, number of shoot, root, and leaf. The result a significant interaction between the concentration of BA, TDZ, and PVP to all observed variables. The combined treatment between 5 mg/L BA, 0.1 mg/L TDZ, and 300 mg/L PVP provided the highest survival rate (100%), growth rate (100%), shoot multiplication (five shoots/explant), and number of leaf (15 leaves/shoot). This medium can be applied for micropropagation of M. acuminata ssp. sumatrana in supplying plant materials for in vitro conservation.</p>

scholarly journals In vitro GROWTH OF ECTOMYCORRHIZAL FUNGI ASSOCIATED WITH Pinus radiata PLANTATIONS IN CHILE

2017 ◽  
Vol 40 (4) ◽  
pp. 415-423
Author(s):  
José-Leonardo García-Rodríguez ◽  
Jesús Pérez-Moreno ◽  
Darcy Ríos-Leal ◽  
Patricia Sáez-Delgado ◽  
Cristian Atala-Bianchi ◽  
...  
Keyword(s):  
Growth Rate ◽  
Ectomycorrhizal Fungi ◽  
Pinus Radiata ◽  
Radial Growth ◽  
Fungal Species ◽  
In Vitro Growth ◽  
Radial Growth Rate ◽  
Mycelial Biomass ◽  
Rhizopogon Luteolus

A comparative study of in vitro growth of three species of ectomycorrhizal fungi (ECMF) (Rhizopogon luteolus, Suillus granulatus and Suillus luteus) was performed. Fungal material was collected in adult Pinus radiata plantations. Isolation and purification of the strains were performed on potato-dextrose-agar medium and the evaluation of the radial growth rate and the increase in mycelial biomass, under different culture conditions, was performed on the Modified Melin Norkrans growth medium. The effects of temperature (24, 28 and 32 ºC) and pH (4.8, 5.3, 5.8, 6.3 and 6.8) of the growth medium were tested for the three fungal species in two independent assays. The results indicate that the temperature had a significant effect on the radial growth rate (RG) and mycelial biomass increase (MB) in all of the evaluated fungal species. The highest RGR and MBI were recorded in R. luteolus, and the lowest values for these variables were registered in S. luteus. Rhizopogon luteolus had the highest sensitivity to pH changes. Meanwhile, there was no pattern in S. granulatus and S. luteus growth response under different pH conditions. When cultivated in vitro, the three studied species of ECMF presented adaptation, exponential, declining and stationary growth phases. The in vitro growth responses recorded in the present study showed the great potential of R. luteolus to be used in future programs using mycorrhizal inoculation in the production of Pinus radiata trees in nurseries in Chile.

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