In Vitro Multiplication of Potato (Solanum tuberosum L.) cv. Cristal II—Microcutting Origin

The potato cultivar Cristal recently released by the CPACT/EMBRAPA Breeding Program has high dry matter and low reduce sugars. These are desirable characteristics as industry processing is concerned. Nevertheless, this is a recalcitrant cultivar. The meristem culture is difficult to establish along with a very low multiplication rate. The aim of this work was to improve the multiplication rate for this cultivar. Two-bud microcuttings derived from apical, mid, and basal regions were inoculated in test tubes with 10 ml MS culture media and vitamins as follows; myo-inositol (100 mg·L–1); sucrose (10 g·L–1). No growth regulator was added. All treatments were placed in a growth room in a 16-hour photoperiod; 25 ± 2°C and 2000 lux. One month later, although it was observed that the final growth was more pronounced for basal microcuttings, no difference could be detected for number of shoots and multiplication rate. It was concluded that it makes no difference whatsoever kind of microcutting is used to start the micropropagation process.

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In Vitro Multiplication of Potato (Solanum tuberosum L.) cv. Cristal under Different MS Salt and Sucrose Concentrations

HortScience ◽

10.21273/hortsci.32.3.471e ◽

1997 ◽

Vol 32 (3) ◽

pp. 471E-471

Author(s):

Gerson R. de L. Fortes ◽

Luciana B. Andrade ◽

Marisa de F. Oliveira ◽

Nilvane T.G. Müller ◽

Janine T. C. Faria

Keyword(s):

Culture Media ◽

Solanum Tuberosum L ◽

Sucrose Concentration ◽

Sugar Content ◽

Potato Cultivar ◽

In Vitro Multiplication ◽

Full Strength ◽

High Dry Matter ◽

The Media

The potato cultivar Cristal has recently been released by the CPACT/EMBRAPA Breeding Program. Such cultivar was selected for having high dry matter and low sugar content, which makes it desirable for the chip industry. However, this is a recalcitrant cultivar as far as in vitro multiplication is concerned. The aim of this work was to improve the rate of multiplication for this cultivar when it was submitted to different MS salt and sucrose concentrations in the culture media. Two-bud microcuttings were inoculated in test tubes (20 × 150) mm with 10 ml MS media at 3/4-, 1/2-, and full-strength and MS vitamins added to: myo-inositol (100 mg·L–1), agar (7.0 g·L–1) and sucrose as follows: 10, 20 and 30 g·L-1. Each treatment was repeated eight times and each replicate had eight explants. After inoculation the whole material was kept in a growth room at 25 ± 2°C, 16-hr photoperiod and 2000 lux. The evaluation was done 35 days later. It was found and increase in the number of buds as the sucrose concentration in the media decreased. As far as MS salts are concerned no difference in bud number was observed. The rate of multiplication was slightly higher for MS media at full strength and sucrose at low concentration (10 g·L–1). This treatment could be recommended for this cultivar.

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114 In Vitro Multiplication of Potato (Solanum tuberosum L.) as Affected by the Microcutting Characteristics

HortScience ◽

10.21273/hortsci.34.3.461b ◽

1999 ◽

Vol 34 (3) ◽

pp. 461B-461

Author(s):

Gerson R. de L. Fortes ◽

Rosilene França ◽

Adriana C. M. Dantas

Keyword(s):

Tissue Culture ◽

Solanum Tuberosum ◽

Light Intensity ◽

In Vitro Culture ◽

Solanum Tuberosum L ◽

Temperate Climate ◽

Multiplication Rate ◽

In Vitro Multiplication ◽

Growth Room

This work was carried out in the Tissue Culture Laboratory of Embrapa Temperate Climate aiming to maximize the protocol for in vitro culture of potato cv. Baronesa. The treatments consisted of multiplication of microcuttings with one, two, or three buds with/without leaves and originated from different regions of the shoot: apical, middle, or basal. Each treatment was repeated five times with each replication composed of five explants that were inoculated in 250-ml flasks with 40 ml of the medium containing MS salts and vitamins added to: sucrose (30 g·L-1), myo-inositol (100 mg·L-1), agar (6 g·L-1). The pH was adjusted to 5.6 before autoclaving. After inoculation, the flasks remained in a growth room at 25 ± 2 °C, 16-h photoperiod, and 19 μmol·m-2·s-1 light intensity provided by cool-white fluorescents lamps. Observations were done every 5 days. Final evaluation was performed after 30 days. It was observed that basal microcuttings provided longer shoots and that microcuttings with leaves bore the best ones. This kind of explant also favored a higher number of shoots, axilary buds, and better multiplication rate. The presence of leaves in the microcutting is important when basal explants are used once it can improve the number of axillary buds and the rate of multiplication. The higher the number of buds in the microcutting the lower the rate of multiplication. The in vitro multiplication of potato could be improved by using one-leaf bud basal microcutting.

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Induksi Kalus Tanaman Kentang Dombu (Solanum tuberosum L.) Secara In Vitro Dengan Pemberian ZPT 2,4-D (Dichlorophenoxy Acetid Acid)

Natural Science Journal of Science and Technology ◽

10.22487/25411969.2019.v8.i2.13538 ◽

2019 ◽

Vol 8 (2) ◽

Author(s):

Siti RLR Idris ◽

Asri Pirade Paserang

Keyword(s):

Solanum Tuberosum ◽

Callus Induction ◽

Culture Media ◽

Callus Formation ◽

Solanum Tuberosum L ◽

Randomized Design ◽

Completely Randomized Design

This research was aimed to determine the effect of the PGR 2.4-D (Dichlorophenoxy Acetid Acid) in various concentrations on induceing callus of Dombu potato (Solanum tuberosum L.). This research was performed based on Completely Randomized Design (RAL) with 6 treatments and 3 times repetation, so there were 18 experiment units. Each experiment used 3 explants so as there were 54 explants. The combination of concentration of the tested PGR in the culture media were T1 = MS0+ 2.4-D 0 ppm (control), T2 = MS0+ 2.4-D 0.5 ppm, T3 = MS0+ 2.4-D 1 ppm, T4= MS0+ 2.4-D 1.5 ppm, T5 = MS0+ 2.4-D 2 ppm and T6 = MS0+ 2.4-D 2.5 ppm. The results showed that callus induction was appeared in the concentration of 1.0, 1.5, 2.0, and 2.5 ppm. Callus was generally formed on the Day-8 after plantation. Callus color was mostly greenish transparent, callus texture was mostly crumb type, and callus formation percentage was almost 100%. The best media for inducing the callus was in treatment T4 (1.5 ppm 2.4-D), it referred to the formed callus biomass

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Efficiency of Transient Expression in Protoplasts of Various Potato Varieties

Biotekhnologiya ◽

10.21519/0234-2758-2020-36-3-16-24 ◽

2020 ◽

Vol 36 (3) ◽

pp. 16-24

Author(s):

S.R. Strelnikova ◽

N.E. Zlobin ◽

P.N. Kharchenko ◽

R.A. Komakhin

Keyword(s):

Solanum Tuberosum ◽

Genome Editing ◽

Transient Expression ◽

Solanum Tuberosum L ◽

Transient Gene Expression ◽

Potato Cultivar ◽

Potato Genome ◽

Technological Stage ◽

Genetic Constructs

Production of mesophilic protoplasts in potato (Solanum tuberosum L.) for transient gene expression is a mandatory technological stage in testing efficiency of new genetic constructs and CRISPR/Cas genome editing. In this study, isolation of protoplasts was carried out from leaves of potato plants of nine Russian cultivars grown in vitro for 6-7 weeks in vessels with foil caps, which prevented gas exchange with the environment. We first ever demonstrated the possibility of obtaining 2.4∙106 to 4.6∙106 viable protoplasts from one gram of aseptic plant leaves depending on the used potato cultivar. We established that the level of transfection of protoplasts with the genetic construct pHBT-sGFP-NosT amounts from 10 to 49% depending on the cultivar, which is sufficient for subsequent successful analysis of the efficiency of genetic constructs and potato genome editing. potato, Solanum tuberosum, protoplasts, plasmid DNA, transfection, transient expression, gfp The work was financially supported by the Comprehensive Research Program «Development of Potato Breeding and Seed Production».

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Interactive effect of genotype and medium on microtuberization of potatoes (Solanum tuberosum L.) grown in vitro

Acta Scientifica Naturalis ◽

10.2478/asn-2020-0003 ◽

2020 ◽

Vol 7 (1) ◽

pp. 8-25

Author(s):

Kadi Zahia ◽

Rached Kanouni Malika ◽

Khammar Sara ◽

Chebout Abderrezeq ◽

Khammar Hichem ◽

...

Keyword(s):

Solanum Tuberosum ◽

First Generation ◽

Culture Media ◽

Solanum Tuberosum L ◽

Interactive Effect ◽

Potato Varieties ◽

Morphological Aspects ◽

Growing Conditions

AbstractThis work investigates the interactive effect of six culture media and three photoperiods (darkness, 16h/8h, 8h/16h) on the microtuberization of four potato varieties (Spunta, Désirée, Kondor and Bartina). The objective is to determine the best tuberization under these growing conditions. The measured parameters which are related to the suitability of the tuberization characteristics are: the morphological aspects (shape, position) and the biometric ones (number and diameter of tubers). The obtained results permit to valorize the meristems that constitute the starting explant, and their good organogenetic skills to provide the first generation of micropropagation with healthy vitroplants in a sufficient quantity. Thus, the grown vitroplants on the medium (MS/2+BAP+COU) presented the best values which are related to the percentage of tuberization, the number of microtubercles / vitroplants and weight of tubers. Moreover, the Bartina genotype showed a remarkable superiority over its media and under a 16h/8h photoperiod except for the diameter of the tubers, where this genotype had the best diameter over the medium (MS/2+KIN) under an 8h photoperiod.

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Respon Pemberian Aspirin dan Kinetin Terhadap Pembentukan Umbi Mikro Kentang (Solanum tuberosum L.) Secara In Vitro

10.25047/agropross.2017.15 ◽

2017 ◽

Author(s):

Yunita Prameswari ◽

FNU Djenal ◽

FNU Damanhuri

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L

Kebutuhan kentang yang semakin tinggi menyebabkan permintaan semakin meningkat. Rendahnya produksi kentang mengakibatkan berbagai upaya untuk peningkatan produksi terus dilakukan. Penggunaan metode kultur jaringan yaitu metode untuk mengisolasi bagian tanaman seperti protoplasma, sel, sekelompok sel, jaringan dan organ dalam kondisi aseptik, sehingga dapat diperbanyak dan beregenerasi menjadi tanaman utuh dapat dijadikan alternatif pemenuhan kebutuhan. Penelitian ini bertujuan untuk mengetahui kecepatan pembentukan umbi mikro kentang (Solanum Tuberosum L.) varietas granola kembang secarain vitro dengan menggunakan dua faktor dan 3 kali ulangan. Faktor pertama yaitu aspirin dengan tiga taraf (5,10,15) ppm. Faktor kedua yaitu kinetin dalam tiga taraf (8,10,12) ppm. Penelitian menggunakan seluruh propagul kentang yang berumur 30 hari setelah subkultur dan data yang didapat dianalisis menggunakan ANOVA. Hasil penelitian menunjukan bahwa interaksi aspirin dan kinetin tidak berpengaruh terhadap jumlah akar, kedinian umbi, dan bobot umbi. Interaksi perlakuan terbaik bagi pembentukan tunas yaitu A2K1 aspirin 10 ppm dan kinetin 8 ppm sedangkan Interaksi perlakuan terbaik pada parameter jumlah umbi yaituA3K2 aspirin 15 ppm dan kinetin 10.

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Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

JURNAL BIOS LOGOS ◽

10.35799/jbl.9.2.2019.24416 ◽

2019 ◽

Vol 9 (2) ◽

pp. 91

Author(s):

Ghea Dotulong ◽

Stella Umboh ◽

Johanis Pelealu

Keyword(s):

Solanum Tuberosum ◽

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Leaf Extract ◽

Solanum Tuberosum L ◽

In Vitro Toxicity ◽

Potato Plants ◽

Colony Diameter ◽

In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

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