scholarly journals An Efficient Method for Callus Culture and Shoot Regeneration of Garlic (Allium sativum L.)

HortScience ◽  
1999 ◽  
Vol 34 (2) ◽  
pp. 348-349 ◽  
Author(s):  
Xabier Barandiaran ◽  
Nieves Martín ◽  
María Fernanda Rodríguez-Conde ◽  
Antonio Di Pietro ◽  
Jesus Martín
Keyword(s):  
Tissue Culture ◽  
Callus Culture ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Efficient Method ◽  
Callus Induction ◽  
Allium Sativum ◽  
Regeneration Process ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

The influence of different callus induction media on the regeneration process in garlic was tested. The auxin 2,4 dichlorophenoxyacetic acid frequently used in garlic tissue culture was found to be detrimental when used at the levels described in the literature. However, combinations of growth regulators commonly used for dicot tissue culture produced high levels of callus induction and regeneration that could be used efficiently in a transformation program.

scholarly journals PERTUMBUHAN KALUS DAUN MELON (Cucumis melo) VARIETAS MAI 119 DENGAN PEMBERIAN BAP (BENZYL AMINO PURINE) DAN 2,4-D (2,4 DICHLOROPHENOXYACETIC ACID)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nining Intan Toharah ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Cucumis Melo ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Benzyl Amino Purine

This study aims to determine the concentration of growth regulators BAP and   2,4-D which have the highest effect in stimulating the formation of callus melon plants (Cucumis melo) Mai 119 variety. Completely randomized design (CRD) was used in this research. Media used on callus induction was MS medium with addition of several concentration of BAP  (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) and 2,4-D (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) either alone or in a combination of both. Parameters measured were the time appearing of callus, callus diameter, callus texture, and callus color. Anova followed by Tukey's test was used to the analyse of time appearing of callus. Data of callus diameter was analyzed using Kruskal Wallis test followed by Mann-Whitney test. In the analysis of parameter related to the callus texture and callus color, descriptive test were used. The results showed that there were differences in the effect of growth regulators on the callus formation. The fastest callus induction and the largest diameter of callus were obtained on media with concentration of 2 mg/L BAP and 3 mg/L BAP.Keywords: BAP (benzyl amino purine), 2,4-D (2,4-dichlorophenoxyacetic acid), callus induction, melon (Cucumis melo) varieties Mai 119

scholarly journals High frequency callus induction and proliferation of MD2 pineapple (Ananas comosus)

Food Research ◽  
2020 ◽  
Vol 4 (S5) ◽  
pp. 115-123
Author(s):  
A.N. Salihan ◽  
N.A. Yusuf
Keyword(s):  
Callus Culture ◽  
Callus Induction ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Ananas Comosus ◽  
Dichlorophenoxyacetic Acid ◽  
Fresh Weight ◽  
Metabolites Production ◽  
2,4 Dichlorophenoxyacetic Acid

Ananas comosus var. MD2 is currently the most preferred pineapple variety in the international market due to its pleasant aroma and high Brix acidity ratio. In vitro approaches such as callus culture is promising in producing disease-free plantlet. However, there are limited studies reported on callus culture of MD2 variety despite the potential of in vitro regeneration through biotechnological advances. The purpose of the study was to determine the effect of plant growth regulators (PGRs) i.e., 2,4- dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and Thidiazuron (TDZ) on callus induction from leaf explant of MD2 pineapple. Leaf base explants were cultured on Murashige and Skoog (MS) media supplemented with varying concentration of 2,4-D (0.5 to 6.0 mg/L) alone and in combination with BAP (1.0 to 3.0 mg/L). The frequency of callus induction was seen significantly highest (91.67±8.33%) with maximum callus fresh weight (0.25±0.07 g) at a combination of 2.0 mg/L 2,4-D and 2.0 mg/L BAP. The shortest duration of callus formation was seen on day 12 with the lowest concentration of 2,4-D, 0.5 mg/L. There is a moderate correlation between the earliness of callus formation and the frequency of callus induction (P<0.01). The most favourable media for callus proliferation was 6.0 mg/L 2,4-D and 2.0 mg/L TDZ as the highest fresh weight of 1.52±0.03 g was recorded. Callus culture has the potential to be a source of plant material and secondary metabolites production. In this study, 2,4-D and BAP have successfully induced callus in MD2 pineapple.

scholarly journals Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D)

2019 ◽  
Vol 7 (2) ◽  
pp. 109
Author(s):  
Azharia Khalida ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Natural Science ◽  
Callus Induction ◽  
Dichlorophenoxyacetic Acid ◽  
Fresh Weight ◽  
Greenish Yellow ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid

The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.

scholarly journals PCIB Enhances Regeneration of Ipomoea cordatotriloba Dennstedt Leaf Explants and Protoplasts

HortScience ◽  
1994 ◽  
Vol 29 (4) ◽  
pp. 327-328
Author(s):  
Ruth S. Kobayashi ◽  
John C. Bouwkamp ◽  
Stephen L. Sinden
Keyword(s):  
Abscisic Acid ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Leaf Explants ◽  
Dichlorophenoxyacetic Acid ◽  
Regeneration Medium ◽  
Ipomoea Cordatotriloba ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Chlorophenoxyisobutyric Acid

Leaf callus of Ipomoea cordatotriloba was initiated by culturing explants on Linsmaier and Skoog medium containing 3 g yeast extract/liter, 18.9 μm ABA, 2.3 μm 2,4-D, and 0.15 m sucrose. Calluses were transferred to Murashige and Skoog media containing 17.8 μm BA and 0, 1, 10, or 100 μm PCIB. The number of shoots from calluses grown on medium containing 10 μm PCIB increased significantly, and the percentage of calluses exhibiting shoot regeneration almost doubled compared to calluses grown on regeneration medium without PCIB. Protoplasts isolated from stem and petiole tissues of in vitro-grown plants were cultured in Kao and Michayluk 8p medium to the callus stage. Calluses (4-6 mm) were transferred to the callus induction and regeneration media used to regenerate leaf-explant callus. Of the protoplast-derived calluses cultured on media containing 10 or 100 μm PCIB, ≈13% and 18%, respectively, regenerated shoots after 2 months; none regenerated on the medium without PCIB. Chemical names used: abscisic acid (ABA); 2,4-dichlorophenoxyacetic acid (2,4-D); N6-benzyladenine (BA); α -p-chlorophenoxyisobutyric acid (PCIB).

scholarly journals Response of Different Explants for Callus Induction in Cucumber

2021 ◽  
Vol 2 (5) ◽  
pp. 71-75
Author(s):  
Hasina Sultana ◽  
Lutfun Nahar ◽  
M. Mofazzal Hossain ◽  
Totan Kumar Ghosh ◽  
Md. Sanaullah Biswas
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Disc ◽  
Dry Weight ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Regenerate Shoot ◽  
2,4 Dichlorophenoxyacetic Acid

In vitro regeneration of cucumber is relatively difficult for genetic improvement. In this regard, different concentrations of growth regulators and three types of explants (cotyledon, hypocotyl and leaf disc) were investigated for their efficiency on callus induction potential. Among different explants explored for callus induction with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), leaf disc responded earlier (4.67 days) and showed higher percentage of callus induction (91.50%) with 2 mg/l 2,4-D supplemented Murashige and Skoog (MS) media. The same concentration of 2,4-D resulted in the maximum callus fresh (0.56 g) and dry weight (0.39 g) from leaf disc explant. Then the callus was transferred to untreated, 2.0 mg/l BAP + 0.2 mg/l NAA + 1.0 mg/l Kn, 2.0 mg/l BAP + 1.0 mg/l NAA + 1.0 mg/l Kn and 2.0 mg/l BAP + 1.5 mg/l NAA + 1.0 mg/l Kn fortified MS medium. After transferring the callus of different explants to shoot regeneration media containing different concentrations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and Kinetin (Kn), only cotyledon callus started to regenerate shoot. The combination of BAP (2 mg/l) + NAA (0.2 mg/l) + Kn (1 mg/l) showed highest shoot regeneration percentage (67.77%) and the maximum number of shoots (5.12) per explant were recorded in the treatment combination of 2 mg/l BAP + 0.2 mg/l NAA + 1 mg/l Kn. These results provided a basis for the optimization of the callus induction protocol of cucumber for genetic transformation.

scholarly journals Pertumbuhan Kalus Rejasa (Elaeocarpus grandiflorus) dari Eksplan Tangkai Daun pada Kondisi Gelap

Life Science ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Nur Wijawati ◽  
Noor Aini Habibah ◽  
Fajar Musafa ◽  
Khoirul Mukhtar ◽  
Y. Ulung Anggraito ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Growth Time ◽  
Dichlorophenoxyacetic Acid ◽  
Independent Variables ◽  
Callus Regeneration ◽  
Dark Conditions ◽  
2,4 Dichlorophenoxyacetic Acid

Rejasa (Elaeocarpus grandiflorus) is a Salatiga identity plant which is now rarely found. Rejasa produces secondary metabolites that have the potential as drugs. This study tested the growth of rejasa callus in the medium with the addition of various types and concentrations of growth regulators. The independent variables used were the type and concentration of growth regulators (2,4-dichlorophenoxyacetic acid (2,4-D) with concentrations of 1.5; 2.5 and 3.5 ppm and Picloram with a concentration of 3.5; 5; and 7.5 ppm). The dependent variable in this study was the growth of callus regeneration (percentage of callus growth, time of callus formation and morphology of callus) observed for five months in dark conditions. Explants used were young petiolus and the medium used in this study was medium Murashige and Skoog (MS). The results showed the lowest percentage of callus induction was found in explants with the addition of Picloram growth regulators with a concentration of 7.5 ppm (14%). Explants maintained in the medium with the addition of Picloram with a concentration of 5 ppm resulted in the highest percentage of callus induction. The time of callus induction is in the range of 10-22 days. The explants with the addition of Picloram growth regulator substances with a concentration of 5 ppm had the best callus induction time, which was 12 days. Most of the callus formed was friable and yellowish. Based on the results of this research, the best medium for callus induction of rejasa in dark conditions was medium with the addition of 5 ppm Picloram.   Rejasa (Elaeocarpus grandiflorus) adalah tanaman identitas Salatiga yang mulai jarang ditemukan. Pertumbuhan populasinya memiliki perkembangan yang lambat. Perkembangan generatif melalui perkecambahan biji terjadi dalam tingkat yang sangat rendah. Dalam kelangkaannya, rejasa memiliki khasiat sebagai tanaman obat melalui metabolit sekunder yang dihasilkan. Penelitian ini menguji pertumbuhan kalus rejasa dalam variasi jenis dan konsentrasi zat pengatur tumbuh. Variabel bebas yang digunakan adalah jenis dan konsentrasi zat pengatur tumbuh (2,4-dichlorophenoxyacetic acid (2,4-D) dengan konsentrasi 1,5; 2,5; dan 3,5 ppm serta pikloram dengan konsentrasi 3,5; 5; dan 7,5 ppm). Variabel terikat dalam penelitian ini adalah pertumbuhan kalus rejasa (persentase tumbuh kalus, waktu berkalus, dan morfologi kalus) dalam medium yang diamati selama 5 bulan. Eksplan yang digunakan adalah tangkai muda yang ditanam dalam medium agar Murashige & Skoog dengan penambahan zat pengatur tumbuh 2,4-D dan pikloram dalam berbagai konsentrasi dan dipelihara dalam kondisi gelap. Hasil penelitian menunjukkan persentase induksi kalus paling rendah terdapat pada eksplan dengan penambahan zat pengatur tumbuh pikloram dengan konsentrasi 7,5 ppm (14%). Eksplan yang dipelihara pada medium dengan penambahan pikloram konsentrasi 5 ppm memghasilkan persentasi induksi kalus tertinggi. Waktu induksi kalus berada dalam rentang 10-22 hari. Eksplan dengan penambahan zat pengatur tumbuh pikloram konsentrasi 5 ppm memiliki rerata waktu induksi kalus paling baik yaitu 12 hari. Kalus yang terbentuk dominan berwarna kekuningan dengan jenis meremah. Berdasarkan hasil penelitiaan, medium yang paling baik untuk induksi kalus dalam kondisi gelap adalah medium MS dengan penambahan zat pengatur tumbuh pikloram konsentrasi 5 ppm.

Evaluation of 10 Canadian barley (Hordeum vulgare L.) cultivars for tissue culture response

1993 ◽  
Vol 73 (1) ◽  
pp. 171-174 ◽  
Author(s):  
A. M. R. Baillie ◽  
K. K. Kartha ◽  
B. G. Rossnagel
Keyword(s):  
Tissue Culture ◽  
Hordeum Vulgare ◽  
Growth Regulators ◽  
Dichlorophenoxyacetic Acid ◽  
Hordeum Vulgare L ◽  
Tissue Culture Response ◽  
Barley Cultivars ◽  
Best Response ◽  
Regenerated Plants ◽  
2,4 Dichlorophenoxyacetic Acid

Ten Canadian barley cultivars — Abee, Deuce, Ellice, Harrington, Manley, Bonanza, Conquest, Duke, Heartland, and Samson — were evaluated for tissue-culture response. Callus was obtained from embryos 3–5 d post anthesis from all cultivars. Fertile plants were regenerated from eight. Abee cultures gave the best response in terms of the number of plants regenerated, while Bonanza and Samson cultures produced no regenerated plants. Heartland and Deuce were selected for further study to determine optimum growth-regulator concentrations for callus production and plant regeneration. Two growth regulators — 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) — were evaluated at five concentrations (0.5, 1.0, 2.5, 5.0 and 10 mg L−1). Maximum regeneration rates were achieved with Gamborg’s B5 medium supplemented with 2.5 mg L−1 2,4-D. Thirty-four Heartland and 19 Deuce regenerants were produced per 100 embryos cultured. Key words: Barley, growth regulators, Hordeum vulgare, regeneration, tissue culture

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

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