Above Ground Biomass Estimation of Syzygium aromaticum using structure from motion (SfM) derived from Unmanned Aerial Vehicle in Paninggahan Agroforest Area, West Sumatra

Above ground biomass (AGB) is all living organic matters above the soil including stem, seed and leaves. This study aimed to estimate the individual clove (Syzygium aromaticum) and it’s above ground biomass using Unmanned Aerial Vehicle in the Agroforestry area in Paninggahan, West Sumatra. This study used a photogrammetry method to calculate trees and estimated the AGB. We detected 257 numbers of trees based on aerial image analysis and observed 270 after we validated on ground check in the field. The result was slightly different between estimated AGB from UAV and observed AGB from our ground validation. The estimated AGB was 5.9 ton/ Ha where the surveyed AGB was 5.6 ton/Ha. The difference between estimated AGB and observed AGB was 0.3 ton/Ha.

Distribution Pattern and Mapping of Invasive Alien Species Bellucia pentamera in Conservation Area of PT. Tidar Kerinci Agung (TKA) Solok Selatan

Bellucia pentamera is one of the most dangerous invasive alien species for environment. This species had been invaded many forests in Indonesia. This species would invade more area due to deforestation. The objectives of this study were to know the mapping and distribution pattern of B. pentamera; to know the effects of distance from road and light intensity to distribution of B. pentamera. This study was conducted from March to August 2020 in Conservation Area of PT. TKA Solok Selatan using belt transect method by plotting 20x50 m2. Data was analyzed using Morishita Index and Linear Regression. Distribution pattern of B. pentamera in PT. TKA was clumped, showed by 1.17 of Morishita index. Seedlings and saplings of B. pentamera were dominant at the edge of conservation forest while trees were distributed from the middle to inside of the forest. Distance from road gave positively effects and significantly towards distribution of B. pentamera showing result 0.702 of R2 and 0.007 of p-Value. Light intensity did not give effects significantly towards distribution of B. pentamera showing result 0.0806 of R2 and 0.427 of p-Value.

Ethnomedicinal Study of the Use of Zingiberaceae by the Mentawai People in Siberut, West Sumatra, Indonesia

The Mentawai archipelago is situated to the west of mainland Sumatra and is part of the West Sumatra province. The Mentawai people are indigenous to this archipelago and are well known for their traditional healing practices performed by their Sikerei healers. Only a few studies on the traditional plant medicines of the Mentawai people have been published, which mostly suggest that Zingiberaceae is one of the most widely used families. This study examines the indigenous knowledge of Zingiberaceae by the Mentawai people living in Siberut. Field surveys were undertaken at four locations in the island where the Sikerei healers were interviewed directly to obtain information about medicinal treatments using plants from the Zingiberaceae family. Voucher specimens were collected, dried and deposited at the Herbarium of Andalas University (ANDA), Padang, West Sumatra. The study suggests that at least 32 Zingiberaceae species are used in the Mentawai’s traditional medicines. The floristic aspects, the plant part used, and the type of disease treated are discussed.

The Response of Callus Formation from Tacca Chantrieri Leaves with Various Concentrations of 2,4-D and BAP by In Vitro

The an annual herbaceous tropical plant which is one of the species of the genus Tacca from the Dioscoreaceae family is Tacca chantrieri. T. chantrieri has a unique inflorescence morphology like that of a bat. The people of Southeast China and Thailand have used by T. chantrieri rhizome as traditional medicine because the methanol extract contains secondary metabolites such as diarylheptanoids, pseudofurostan, withanolide, taccalonolide, and saponins. To maintain its sustainability, it is necessary to propagate T. chantrieri by using in vitro culture techniques such as callus culture. The purpose of this study was to determine the response of T. chantrieri leaf callus formation and to determine the optimal concentration with various concentrations of 2,4-D and BAP in vitro. This study used a completely randomized design consisting of control treatments, 1 and 2 mg L-1 2,4-D and concentrations of 0.5; 1.0; 1.5; 2.0 2,4-D combined with 3 mg L-1 BAP. The observations were made for 60 days after planting. The results showed that the concentration of 1.5 mg L-1 2,4-D + 3 mg L-1 BAP affected the percentage of live explants and the percentage of callus formation by 100% respectively, and the time of callus appeared 18.75 days after planting.

Bioassessment of Batang Kandis River Water Quality Using Macrozoobenthos in Koto Tangah district, Padang City

This study was conducted from July to December 2019 which was aimed to determine the composition of macrozoobenthic community and to assess the water quality of the Batang Kandis river based on the BMWP ASPT index. A survey method was used in this study. The study site was determined by purposive sampling based on environmental condition which was divided into four stations. Macrozoobenthos samples were taken using a surber net (30 x 30 cm2). The physical and chemical factors of water condition were measured during sampling time. The results showed that 23 genera of macrozoobenthic communities were found in the Batang Kandis river which were consisted of two classes i.e., Hirudinea (1 order, 1 family, 1 genus) and Insect (7 orders, 14 families, 22 genera). These macrozoobenthic communities had an average density ranges from 220.00 - 706.67 ind. m-2 with the highest density was found at Station II and the lowest one at Station IV. Variation in the predominant genus was found at every station: Orthocladius and Elophila (station I); Polypedilum, Orthocladius, Elophila, and Hydropsyche (station II); Polypedylum, Macropelopia, and Caenis (station III); and Polypedylum, Orthocladius, and Caenis (station IV). The water quality of Batang Kandis river was classified into not polluted (station I, II and III) to slightly polluted (station IV) based on the BMWP-ASPT indexes.

Diversity of Bivalvia, Gastropoda and Holothuroidea in Intertidal Zone of North Javan Sea Coastal, Indonesia

This study aims to know diversity of Bivalvia, Gastropoda and Holothuroidea. Measures used in this study were Density Index (D), Diversity Index (H’), Evennes Index (E’) dan Important Value Index (INP). This study used transect sampling method by 1 × 1 meter quadrant. Target species of this study were member of Bivalvia, Gastropoda and Holothuroidea that inhabit in intertidal zone of north Javan Sea coastal. This intertidal zone was divided into 3 zone; zone I is 50 meter, zone II is 75 meter and zone III is 100 meter from coastal line to sea. Total sample of this species were 1064 individual, that divided into 7 species of Bivalvia, 10 species of Gastropoda and 2 spesies of Holothuridea. The highest Diversity Index was for Gastropoda in zone I (253 individual/m2). Diversity Index was in moderate category (1< H’ < 2) while the zone III was the highest. Evennes Index of all zones were relatively similar. The highest Important Value Index was species of Clypeomorus clypeomorus, that has value 53,06%.

Addition of Benzyl Amino Purine (BAP) to Induction of Drendan Seeds Buds (Lansium domesticum var. aqueum (Jack) Miq.) by In Vitro

Drendan (Lansium domesticum var. aqueum (Jack) Miq. is one of Indonesia's native tropical fruits. Drendan has high antioxidant activity and contains high nutrients, but has started to rarely be found in the field because its management and cultivation is rarely done due to its sour taste in vitro culture is a method or technique of plant propagation which can be an alternative to obtaining drendan seedlings in large quantities and in a short time vegetatively. The purpose of this study was to determine the effect of BAP on shoot induction from drendan seed explants and determine the best concentration of BAP on shoot growth of drendan seed explants in vitro. This study used a completely randomized design with BAP concentrations of 0, 1, 3, 5 and 7 mg / L BAP. The result of this research is the addition of BAP has been able to increase the percentage of live explants and the percentage of shoot formation. Giving BAP did not significantly affect the number of shoots and had a significant effect on shoot emergence time and shoot length. The addition of BAP resulted in slower shoot emergence time. Giving BAP was not able to stimulate shoot induction, but the addition of BAP could increase shoot length growth at a concentration of 5 mg / L BAP of 0.52 cm.

Antimicrobial Activity of Skin Secretion of Rana hosii Frog (Anura: Ranidae) against several pathogenic microbes

The research on antimicrobial activity of skin secretion derived from Rana hosii (Anura: Ranidae) against several pathogenic microbes had been conducted at Microbiology Laboratory and at Genetics and Biomolecular Laboratory, both in the Biology Department, Universitas Andalas. The research used to survey and experimental methods. The study aimed to determine the effectivity of Rana hosii skin secretion to inhibit microbial activity, hence it tested onto some pathogenic microbes such as Escherichia coli, Staphylococcus aureus and Candida albicans. The results showed that the biggest inhibition zone formed against E. coli was 10.96 + 0.63 mm, on S. aureus was 11.74 + 1.00 mm, while on C. albicans was 7.42 + 0.80 mm. The results indicated that the skin secretions from Rana hosii frog could be a potential broad-spectrum antibacterial, and antifungal activity against C. albicans.

Plant Breeding Through Protoplast Fusion

Protoplast culture (protoplast fusion) is one method of tissue culture that is widely used in plant breeding programs in a relatively short time. This method is used to overcome the problem of plants that are difficult or impossible to cross conventionally as well as used for species improvement by transferring the desired gene from the donor plant to the target plant via protoplast fusion. Protoplast fusion makes it possible to produce plants that are resistant to a disease and various abiotic stresses, rapid growth rates and have a better quantity and quality of metabolites than their parents. Various factors affect the success of fusion and regeneration of protoplasts into whole plants, including the source of explants, the composition of the enzyme solution and the duration of incubation, fusagen type and culture media for regeneration.

The Growth of Coastal Cottonwood (Hibiscus tiliaceus Linn.) Seedlings by Inoculating Arbuscular Mychorrhiza Fungi (AMF) on Sand Beach Planting Media

Hibiscus tiliaceus L. is a seaside plant that has several benefits, one of which is as a producer of paper fiber. H. tiliaceus need a better nutrient to grow on coastal land that is still constrained by the high level of salinity. By this, a special treatment is needed to support its growth. One of them is the application of mycorrhizal inoculants. Mycorrhizae is known as one of the fungi that can help plants tolerate salinity. The aims of the research were to determine the effect of AMF inoculants and determine the best doses of AMF to the seedling growth. The research used completely randomized design with 5 treatments and 5 replications. The treatments were (A) without AMF (control), (B) inoculation AMF 10 g/plant, (C) inoculation AMF 15 g/plant, (D) inoculation AMF 20 g/plant and (E) inoculation AMF 25 g/plant. The result showed that AMF can infect the roots of hibiscus seedlings with an infection degree of 18-34%. However, it has not been able to have a significant effect on shoot length, number of leaves, dry weight of root, and dry weight of shoot.