scholarly journals Adventitious Rooting of Stem Cuttings of Yellow-flowered Magnolia Cultivars is Influenced by Time after Budbreak and Indole-3-butyric Acid

HortScience ◽  
2006 ◽  
Vol 41 (1) ◽  
pp. 202-206 ◽  
Author(s):  
Jyotsna Sharma ◽  
Gary W. Knox ◽  
Maria Lucia Ishida

Certain cultivars of magnolia are desirable in landscapes for their uncommon yellow flowers. While cultivars derived from Magnolia acuminata L. (cucumbertree magnolia) are difficult to propagate by stem cuttings, some with mixed parentage appear easier to propagate in this manner. We propagated six yellow-flowered cultivars vegetatively by applying 0, 8, 16, or 30 g·kg–1 (0, 8,000, 16,000, or 30,000 ppm) indole-3-butyric acid (IBA) in talc to bases of terminal stem cuttings collected 5, 7, 9, or 11 weeks after budbreak. Mean rooting percentage over all cultivars increased from 12% (in the absence of IBA) to 34% (after application of 30 g·kg–1 IBA). Rooting percentage and basal stem diameter of a cutting did not seem related. For each collection date, more cuttings of `Ivory Chalice' and `Yellow Lantern' developed roots than the other cultivars. More roots (mean = 5) developed on cuttings of `Yellow Lantern' collected 5 weeks after budbreak or when treated with 30 g·kg–1 IBA than the other cultivars. `Butterflies' largely remained unresponsive, whereas rooting of `Golden Sun,' `Hot Flash,' and `Maxine Merrill' collected 5 weeks after budbreak was 31%, 22%, and 28%, respectively. When data were analyzed separately for selected cultivars, 63% rooting was observed among cuttings of `Ivory Chalice' collected 7 weeks after budbreak. Rooting percentage was higher (22%) among cuttings of `Hot Flash' collected 5 or 7 weeks after budbreak in comparison to later collection dates, but harvest date did not influence rooting of `Yellow Lantern,' which ranged from 44% to 59%. Collection of stem cuttings early in the growing season (5 weeks after budbreak) was beneficial (31% rooting) for inducing rooting among cuttings of `Golden Sun.' We conclude that `Ivory Chalice' and `Yellow Lantern' are promising choices for growers interested in clonal propagation of yellow-flowered cultivars of magnolia. To maximize rooting among these cultivars, terminal cuttings should be collected within 5 to 11 weeks after budbreak and treated with 16 or 30 g·kg–1 IBA in talc. Early collection dates (5 to 7 weeks after budbreak) improved rooting among cuttings of other cultivars but these, particularly `Butterflies,' remain variably recalcitrant and merit further study.

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1001A-1001
Author(s):  
Jyotsna Sharma ◽  
Gary Knox ◽  
Maria Ishida

We propagatedsixyellow-flowered cultivars of Magnolia vegetatively by applying 0, 8, 16, or 30 g·kg-1 indole-3-butyric acid (IBA) in talc to bases of terminal stem cuttings collected 5, 7, 9, or 11 weeks after budbreak. Mean rooting percentage increased from 12% (in the absence of IBA) to 34% (after applying 30 g·kg-1 IBA). Rooting percentage also increased with increasing basal caliper (r2 = 0.25; P< 0.0001) of a cutting. For each collection date, more cuttings of `Ivory Chalice' and `Yellow Lantern' developed roots than did other cultivars. When data were analyzed separately for selected cultivars, 63% rooting was observed among cuttings of `Ivory Chalice' collected 7 weeks after budbreak. Rooting percentage was higher (22%) among cuttings of `Hot Flash' collected 5 or 7 weeks after budbreak in comparison to later collection dates, but harvest date did not influence rooting, which ranged from 44% to 59%, among cuttings of `Yellow Lantern'. Collection of stem cuttings early in the growing season (5 weeks after budbreak) was beneficial (31% rooting) for inducing root formation among cuttings of `Golden Sun'. We conclude that `Ivory Chalice' and `Yellow Lantern' are promising choices for growers interested in clonal propagation of yellow-flowered cultivars of Magnolia. To maximize rooting, terminal cuttings should be collected within 5 to 11 weeks after budbreak and should be treated with 16 or 30 g·kg-1 IBA in talc. Early collection dates improved rooting frequencies among cuttings of other cultivars but these, particularly `Butterflies', remain variably recalcitrant and merit further study.


2018 ◽  
Vol 12 (2) ◽  
pp. 491-499
Author(s):  
Aurea Portes Ferriani ◽  
Cícero Deschamps ◽  
Wanderlei Do Amaral ◽  
Luiz Everson da Silva

Native Piper species present significant repellent, antimicrobial, inseticidal, anti-tumor and anti-protozoal biological activities. Studies on new species can discover unpublished potentialities and vegetative propagation for the development of cultivation protocols and reduce the natural extraction. The objective of this study was to evaluate the rooting of P. arboreum, P. cernuum and P. diospyrifolium stem cuttings with different treatments containing doses of indole butyric acid (0, 500; 1,000; 1,500; 2,000 and 3,000 mg L-1). Branches were collected in the “Bom Jesus Biological Reserve”, Parana state, Brazil. The cuttings, with a length of 12 cm and average diameter of 6 mm, were placed in 53 cm3 plastic tubes with the commercial substrate Tropstrato HP® and intermittent misting. After 90 days, the survival, mortality, cuttings with new shoots, number and length of the three main roots were evaluated. The percentages of rooting reached an average of 67.5% for P. arboreum, 51.6% for P. cernuum and 50.4% for P. diospyrifolium. A positive effect in the treatments containing the plant regulator was observed for rooting development, percentage of cuttings with shoots and number of roots per propagule, but there were variations in the responses of each species. Leaf retention was an important factor for the adventitious rooting formation in all evaluated species.


HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Benjamin E. Deloso ◽  
Anders J. Lindström ◽  
Frank A. Camacho ◽  
Thomas E. Marler

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.


1985 ◽  
Vol 15 (6) ◽  
pp. 1172-1176 ◽  
Author(s):  
Farrell C. Wise ◽  
Frank A. Blazich ◽  
L. Eric Hinesley

Effects of collection date, crown position, and indole-3-butyric acid concentration on rooting and subsequent vegetative growth of softwood cuttings taken from 14-year-old Fraser fir (Abiesfraseri (Pursh) Poir.) Christmas trees were studied. Indole-3-butyric acid levels producing maximum rooting (approximately 50%) varied with crown position and date of severance. Abaxial bending during rooting was greatest for cuttings from the first collection (June 23). Increasing indole-3-butyric acid concentrations decreased abaxial bending of cuttings from the first two severance dates. One year after rooting, plagiotropism was still most pronounced on cuttings collected the first date as a result of active bending of the original cutting segment. Terminal shoot growth was greatest for cuttings from the first and third collections. Lateral budbreak was superior on cuttings collected on the first date. When all factors are taken into consideration, the best combination of rooting and vegetative growth was obtained from upper-crown cuttings which were collected just prior to lignification, wounded, and treated with 1500 ppm indole-3-butyric acid. After one season (one growth flush), rooted softwood cuttings were comparable to 2-year-old plants (two flushes) propagated by hardwood cuttings. The temporal separation of rooting from shoot extension may have been responsible for improving initial vegetative growth.


2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.


2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.


HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 884d-884
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
John M. Ruter

Stem cuttings of golden euonymus (Euonymus japonicus `Aureo-marginatus'), shore juniper (Juniperus conferta `Blue Pacific'), white indian hawthorn (Rhaphiolepis indica `Alba'), and `Red Cascade' miniature rose (Rosa `Red Cascade') were successfully rooted in plugs of a stabilized organic substrate that had been soaked in aqueous solutions of the potassium salt of indole-3-butyric acid (K-IBA) at 0 to 75 mg·L–1 before inserting the cuttings. Cuttings were rooted under intermittent mist in polyethylene-covered greenhouses with rooting periods appropriate for each species. Rooting percentages showed some increase with increasing auxin concentration with juniper cuttings, but were similar among treatments for the other three species. Number of roots per rooted cutting increased with increasing auxin concentration with cuttings of juniper, Indian hawthorn, and rose, and was greatest using around 60 mg·L-1 K-IBA for cuttings of juniper and Indian hawthorn and 30 to 45 mg·L-1 K-IBA for cuttings of rose.


HortScience ◽  
2003 ◽  
Vol 38 (1) ◽  
pp. 92-96 ◽  
Author(s):  
Janine G. Haynes ◽  
John M. Smagula ◽  
Paul E. Cappiello

Stem cuttings were harvested in April from four clones of containerized bunchberry(Cornus canadensis L.) forced in the greenhouse and in June from the same four clones growing in the field. April cuttings that had produced rhizomes by transplant time produced the greatest mean number and weight of shoots during the first growing season compared to April cuttings without rhizomes, June cuttings with rhizomes, or June cuttings without rhizomes. In a second study, cuttings and single-stem divisions were taken in July; divisions produced a greater mean number of shoots than did stem cuttings when compared at the end of Oct. A third study evaluated the effect of K-IBA application to lateral buds on subsequent rhizome production, and the effect of cutting node number (two vs. three nodes) on root or rhizome development. Treating lateral buds with K-IBA was not inhibitory to rhizome formation and elongation. Compared to two-node cuttings, three-node cuttings produced greater mean rootball size, rhizome number, and rhizome length; nearly twice as many of the three-node cuttings formed rhizomes as did two-node cuttings. A fourth study showed that cuttings rooted for 5 or 6 weeks in a mist enclosure generally exhibited greater shoot and rhizome production by the end of the first growing season than cuttings rooted for 8 or 9 weeks. This was despite the finding that cuttings rooted for longer durations (8 or 9 weeks) possessed larger rootballs and greater rhizome numbers at transplant time compared to cuttings rooted for shorter durations (5 to 6 weeks). Chemical name used: indole-3-butyric acid (K-IBA).


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