scholarly journals Three Mussaenda Cultivars Propagated by Stem Cuttings Exhibit Variation in Rooting in Response to Hormone and Rooting Conditions

2000 ◽  
Vol 10 (4) ◽  
pp. 780-784
Author(s):  
Rolston St. Hilaire ◽  
Carlos A. Fierro Berwart
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Clonal Propagation ◽  
Stem Cuttings ◽  
Root Number ◽  
Root Numbers ◽  
Rooting Medium ◽  
Rooting Of Cuttings

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.

scholarly journals Indole -3- Butyric Acid Induces Plant Regeneration From Stem Cuttings Of Three Medicinal Plants

2020 ◽  
Vol 37 (1) ◽  
pp. 109-121
Author(s):  
C.U. Okafor ◽  
E.U. Njoku ◽  
F.C. Ike ◽  
C.C. Onyekwuluje
Keyword(s):  
Medicinal Plants ◽  
Butyric Acid ◽  
Survival Rates ◽  
Control Treatment ◽  
Stem Cuttings ◽  
Root Number ◽  
Growth And Survival ◽  
Root Numbers ◽  
Significant Difference ◽  
Leaf Formation

Field regeneration of three (3) medicinal plants - Securidaca longepedunculata (violet tree), Ocimum gratissimum (scent leaf) and Pterocarpus mildebraedii by means of rooting of stem cuttings at different lengths of 10 -15 cm and 15 -20 cm were studied. The effect of the plant hormone Indole-3-Butyric acid (IBA) at 5g/l was tested on rooting, bud sprout and leaf formation of the rooted stem cuttings of the plant species as well as the effect of the length of the stem cuttings on their growth and survival rates. The results of the study revealed that stem cuttings of O. gratissimum treated with IBA and the control treatment sprouted within 5 - 8 days. The treated O. gratissimum stem cuttings produced a slightly higher number of buds (2.58 ± 0.86) when compared to the control (2.00 ± 0.89) with no significant difference at P ≤ 0.05. Stem cuttings of the treated P. mildbraedii cuttings sprouted within 8 -10 days with 1.40 ± 0.37 number of buds, with the control showing no sprouts. Consequently, IBA application produced more leaves (13.00 ± 5.58) and roots (1.33 ± 0.01) than the control. However, O. gratissimum stem cuttings had the highest leaf (13.08 ± 4.47) and root numbers (135.00 ± 13.45) followed by the control with values- 13.00 ± 5.58 for leaf development and 61.66 ± 2.34 for root number respectively. Irrespective of the treatments, none of the stem cuttings of S. longepedunculata sprouted. This study showed that exogenous application of IBA to stem cuttings of the tested plants, except S. Longepedunculata, improved the root number, number of leaves and number of buds. Keywords: Stem cuttings, Indole-3-Butyric acid (IBA), Rooting

scholarly journals Propagation of Thuja x ‘Green Giant’ by Stem Cuttings: Effects of Growth Stage, Type of Cutting, and IBA Treatment

1998 ◽  
Vol 16 (4) ◽  
pp. 212-214
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney
Keyword(s):  
Root Length ◽  
Growth Stage ◽  
Growth Stages ◽  
Stem Cuttings ◽  
Root Number ◽  
Stock Plant ◽  
Indolebutyric Acid ◽  
First Order ◽  
Rooting Medium ◽  
Plant Growth Stages

Abstract Stem cuttings of Thuja L. x ‘Green Giant’ (‘Green Giant’ arborvitae), consisting of 22 cm (8.6 in) terminals (tips of first-order laterals) or 20 cm (7.8 in) laterals (side shoots removed from those portions of terminal cuttings inserted into the rooting medium), were collected on three dates associated with specific stock plant growth stages (softwood, semi-hardwood, and hardwood) and treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 9000 ppm (0.9%). Regardless of cutting type or auxin treatment, cuttings rooted in high percentages at each growth stage. Overall rooting for softwood, semi-hardwood, and hardwood, cuttings was 85%, 86%, and 96%, respectively. Semi-hardwood cuttings were the only cuttings in which percent rooting was affected by IBA treatment or cutting type, with lateral cuttings rooting in higher percentages than terminal cuttings (92% vs. 79%, respectively), while IBA concentrations of 3000 (0.3%) or 6000 ppm (0.6%) proved optimal for both cutting types. Mean root number and mean root length varied by growth stage, cutting type, and IBA concentration. In general, rooting percentages and root length were optimized at the hardwood stage with either cutting type treated with 3000, 6000 or 9000 ppm (0.3%, 0.6% or 0.9%) IBA, resulting in >10 roots per cutting.

scholarly journals Cutting Type and IBA Treatment Duration Affect Teucrium fruticans Adventitious Root Quality

2014 ◽  
Vol 42 (2) ◽  
pp. 478-481 ◽  
Author(s):  
Leo SABATINO ◽  
Fabio D’ANNA ◽  
Giovanni IAPICHINO
Keyword(s):  
Acid Solution ◽  
Butyric Acid ◽  
Root Development ◽  
Root Length ◽  
Treatment Duration ◽  
Acid Treatment ◽  
Stem Cuttings ◽  
Root Number ◽  
Rooted Cuttings ◽  
Root Quality

Root development of stem cuttings of Silver Germander (Teucrium fruticans) was investigated in relation to cutting type and indole-3-butyric acid (IBA) treatment. Terminal cuttings of a clone grown in Sicily were trimmed to three types: terminal cuttings with apex (TWA), terminal cuttings without apex (TWOA) or sub-terminal cuttings (ST). To verify the cutting response to exogenous auxin, cuttings were dipped to a 2.0 cm depth in a 0.5% indole-3-butyric acid solution for 0, 5 or 7 minutes. Overall percent survival was 97 to 98%. Rooting percent, root number and root length were affected by cutting type and indole-3-butyric acid treatment. In general, TWA cuttings demonstrated a higher capacity to form roots than cuttings without apex (TWOA and ST cuttings). In absence of indole-3-butyric acid treatment, TWA and ST cuttings gave higher rooting percentages than TWOA cuttings. Exposing cuttings to indole-3-butyric acid improved percent rooting, number of roots per cutting and root length. The best results in terms of rooting percentage and root number per cuttings were obtained with TWA cuttings in combination with 7 min indole-3-butyric acid basal dip. However, cuttings taken farther down the stem, such as sub-terminal cuttings gave satisfactory rooting performance as well. We suggest that the use of all cutting types tested associated to indole-3-butyric acid basal dip for 5 or 7 min may be beneficial to propagators wishing to produce T. fruticans rooted cuttings with well-developed root system.

scholarly journals Rooting Response of Stem Cuttings of Shantung Maple (Acer truncatum) to Time of Year, Cutting Position, and Auxin Concentration, Formulation, and Solvent

2014 ◽  
Vol 32 (3) ◽  
pp. 163-166
Author(s):  
Justin A. Brock ◽  
Jason J. Griffin
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Stem Cuttings ◽  
Root Number ◽  
Solvent Water ◽  
Auxin Concentration ◽  
Time Of Year ◽  
Rooting Response

The influence of time of year, cutting position, and auxin concentration, formulation, and solvent on rooting of stem cuttings of shantung maple (Acer truncatum Bunge) was investigated in seven experiments. Softwood, semi-hardwood, second-flush softwood, and hardwood stem-tip cuttings were compared. Semi-hardwood cuttings [15 cm (6 in) in length] rooted best (55%). Auxin treatments [indole-3-butyric acid (IBA) or the potassium (K) salt of IBA (K-IBA)] ranged from 0 to 15,000 ppm (0 to 1.5%). Generally, rooting percentage decreased as auxin concentration increased. Cutting position (terminal or subterminal), auxin formulation (liquid or powder), and auxin solvent [water or ethanol:water (1:1 v/v)] did not affect percent rooting. Mean root number and mean root length were unaffected by all treatments. Results suggest shantung maple roots best from semi-hardwood cuttings treated with auxin at concentrations of 0 or 2,500 ppm (0.25%).

scholarly journals   Alanine conjugate of indole-3-butyric acid improves rooting of highbush blueberries

2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi
Keyword(s):  
Butyric Acid ◽  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Stem Cuttings ◽  
Practical Application ◽  
Rooting Of Cuttings ◽  
Highbush Blueberries ◽  
Set Up ◽  
Indole 3 Acetic Acid

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry.  

Effect of Indole-3-Butyric Acid on Clonal Propagation of Mulberry (Morus alba L.) Stem Cuttings: Rooting and Associated Biochemical Changes

2015 ◽  
Vol 87 (1) ◽  
pp. 161-166 ◽  
Author(s):  
Azamal Husen ◽  
Muhammad Iqbal ◽  
Shadab Naseer Siddiqui ◽  
Sayed Sartaj Sohrab ◽  
Getinet Masresha
Keyword(s):  
Butyric Acid ◽  
Clonal Propagation ◽  
Morus Alba ◽  
Biochemical Changes ◽  
Stem Cuttings

scholarly journals Effects of Auxin Concentration and Medium Temperature on Four Woody Plant Taxa Propagated by Stem Cuttings using Recirculating Subirrigation in a Growth Chamber

2003 ◽  
Vol 21 (3) ◽  
pp. 126-130
Author(s):  
James S. Owen ◽  
William A. Johnson ◽  
Brian K. Maynard
Keyword(s):  
Root Formation ◽  
Woody Plant ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Number ◽  
Medium Temperature ◽  
Root Numbers ◽  
Auxin Treatment ◽  
Auxin Concentration ◽  
Propagation Medium

Abstract Four woody plant taxa (‘Sparkleberry’ holly, ‘Mariesii’ viburnum, ‘Shasta’ viburnum, and Red Sunset® maple) were propagated from softwood stem cuttings in a recirculating subirrigation propagation system to evaluate the effect on adventitious root formation of three auxin (Dip-n-Grow®) concentrations [0 (water), 20: 1, or 10: 1] and three medium temperatures [20C (68F), 23C (74F), or 26C (79F)]. All taxa showed a positive rooting response to auxin treatment. Compared to cuttings treated with water, root number of all taxa doubled when treated with the highest auxin concentration (10: 1 Dip-n-Grow® dilution). Length of the longest root of rooted cuttings increased with auxin treatment of two taxa. Medium temperature also affected rooting of all taxa. The intermediate temperature evaluated, 23C (74F), was optimal for all rooting parameters on cuttings of ‘Mariesii’ viburnum, and for root length and percentage rooting on cuttings of ‘Shasta’ viburnum and Red Sunset® maple. A propagation medium temperature of 26C (79F) resulted in the greatest root number on cuttings of ‘Shasta’ viburnum, while 20C (68F) was optimal for root number on cuttings of Red Sunset® maple. Root numbers on cuttings of ‘Mariesii’ viburnum were reduced at 20C (68F) and 26C (79F), which appeared to be sub-and supra-optimal temperatures, respectively, for root initiation. All rooting parameters on cuttings of ‘Sparkleberry’ holly increased with propagation medium temperature. Temperature-auxin interactions were apparent for root number and length on cuttings of ‘Mariesii’ and ‘Shasta’ viburnum. The recirculating subirrigation system was useful for evaluating the basal temperature and auxin dose interactions and was effective for rooting softwood stem cuttings without intermittent mist irrigation.

scholarly journals Comparison of Rootability of Stem Cuttings from Seedlings of Aesculus sp. and Mature Aesculus × arnoldiana ‘Autumn Splendor’

1988 ◽  
Vol 6 (2) ◽  
pp. 69-71
Author(s):  
B.A. Bergmann ◽  
W.P. Hackett ◽  
H. Pellett
Keyword(s):  
Butyric Acid ◽  
Developmental Stages ◽  
Stem Cuttings ◽  
Mature Tree ◽  
Mature Trees ◽  
Old Growth ◽  
Rooting Of Cuttings ◽  
Root Suckers ◽  
Stem Segments

Rootability was evaluated for stem cuttings taken at varying positions and developmental stages from seedlings of Aesculus and from the mature trees of Aesculus × arnoldiana ‘Autumn Splendor’. Rooting was 100% for cuttings taken from 2-week-old seedlings and 0% for those obtained from the crown of the mature tree 2 months after commencement of spring growth flush. Intermediate rootability was found for cuttings taken from 1-month-old seedlings and from the 2 week-old growth of suckers at the base of the mature tree. Rooting of cuttings with intermediate rooting potential was increased by a quick dip treatment with 2500 ppm indole-3-butyric acid (IBA) or by using apical rather than basal stem segments. Commercially acceptable rooting was not achieved using cuttings from root suckers or the crown of Aesculus ‘Augumn Splendor’.

scholarly journals Shading and IBA Treatment Do Not Improve Rooting of Stem Cuttings of Quercus phillyraeoides ‘Emerald Sentinel’

1999 ◽  
Vol 17 (3) ◽  
pp. 123-125
Author(s):  
Jason J. Griffin ◽  
Frank A. Blazich ◽  
Thomas G. Ranney
Keyword(s):  
Solar Irradiance ◽  
Root Length ◽  
Growth Stage ◽  
Shoot Tip ◽  
Stem Cuttings ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Rooted Cutting ◽  
Quercus Phillyraeoides

Abstract Shoot tip cuttings of Quercus phillyraeoides A. Gray ‘Emerald Sentinel’ (‘Emerald Sentinel’ oak), in a transitional growth stage between softwood and semi-hardwood, were collected from containerized plants growing under uniform fertility on June 4, 1998. Cuttings were treated with talc formulations of indolebutyric acid (IBA), ranging from 0 to 8000 ppm (0.8%), and placed under intermittent mist with shade levels excluding 0%, 30%, 60% or 90% of full solar irradiance. After 14 weeks, percent rooting averaged 18% and was unaffected by either IBA concentration or shading regime. Similarly, both mean root number per rooted cutting and mean root length were unaffected by IBA concentration or shading. Roots per rooted cutting and mean root length averaged 2.6 and 41 mm (1.6 in), respectively. Results suggest that shading stem cuttings of ‘Emerald Sentinel’ during propagation does not affect rooting.

scholarly journals Indolebutyric acid on boxwood propagation by stem cuttings

2018 ◽  
Vol 24 (4) ◽  
pp. 347-352 ◽  
Author(s):  
Leandro Marcolino Vieira ◽  
Silvano Kruchelski ◽  
Erik Nunes Gomes ◽  
Katia Christina Zuffellato-Ribas
Keyword(s):  
Statistical Difference ◽  
Callus Formation ◽  
Adventitious Rooting ◽  
Commercial Production ◽  
Stem Cuttings ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Significant Percentage ◽  
Root Numbers ◽  
Buxus Sempervirens

Buxus sempervirens L., popularly known as boxwood, is a woody shrub widely used in gardening and landscaping. One of the most important factors for B. sempervirens commercial production is its propagation. The objective of this study was to evaluate the effect of indolebutyric acid (IBA) concentrations on the adventitious rooting of semihardwood stem cuttings of the species. Branches with new shoots were collected from stock plants with approximately 17 years of growth. Stem cuttings of six centimeters in length with two leaves in the upper region were made with a straight cut at the apex and a bevel cut at the base. Cuttings bases were immersed for 10 seconds in hydroalcoholic solutions (50% v v-1) with IBA at 0, 1500, 3,000 or 6,000 mg L-1. The cuttings were evaluated at 64 and 116 days after planting. Rooting, mortality, callus formation and sprouting percentages as well as root number and root length were measured. At 64 days, no significant percentage of rooting was observed in B. sempervirens stem cuttings and sprouting and callus formation were not significantly affected by the treatments. Keeping the cuttings in the greenhouse for 116 days led to rooting rates up to a 97.5%, with no statistical difference among treatments. Root lengths and root numbers did not differ as a function of treatments. The required period for B. sempervirens stem cuttings rooting is around 116 days; IBA application does not affect rooting, survival or sprouting of B. sempervirens stem cuttings.

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