scholarly journals Expressed Sequence Tags (ESTs) from a Jute (Corchorus olitorius) cDNA Library

1970 ◽  
Vol 16 (2) ◽  
pp. 95-104 ◽  
Author(s):  
J Matthew Taliaferro ◽  
Ahmad S Islam ◽  
Kanagasabapathi Sathasivan
Keyword(s):  
Arabidopsis Thaliana ◽  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Higher Plants ◽  
Open Reading Frame ◽  
Sequence Information ◽  
Corchorus Olitorius ◽  
Reading Frame ◽  
Partial Cdna ◽  
Expressed Sequence

Analysis of the genome of jute (Corchorus olitorius) was done by creating a new cDNA library of expressed sequence tags (ESTs) in pBluescript as the previous libraries reported earlier in this journal yielded only small DNA fragments from chloroplast and mitochondrial DNA. This report discusses results from a cDNA library constructed using poly A+ mRNA purified from 7-day old etiolated jute seedlings. Out of 700 recombinant plasmids obtained, 250 were analyzed using WU-BLAST (www.arabidopsis.org) for similar EST sequences in Arabidopsis thaliana and other higher plants. So far the analysis of the library has yielded several significant sequences, including the complete open reading frame of the 60S acidic ribosomal protein P3 and a partial cDNA of Class I chitinase. These results and future EST sequences from this library will be made available in Genbank and the sequence information will be used to clone full length DNA through PCR.  DOI = 10.3329/ptcb.v16i2.1110Plant Tissue Cult. & Biotech. 16(2): 95-104, 2006 (December)

Characterization of open reading frame-expressed sequence tags generated from Bos indicus and B. taurus mammary gland cDNA libraries

2004 ◽  
Vol 35 (3) ◽  
pp. 213-219 ◽  
Author(s):  
A. F. Mota ◽  
T. S. Sonstegard ◽  
C. P. Van Tassell ◽  
L. L. Shade ◽  
L. K. Matukumalli ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Expressed Sequence Tags ◽  
Bos Indicus ◽  
Open Reading Frame ◽  
Cdna Libraries ◽  
Reading Frame ◽  
Expressed Sequence

scholarly journals Construction of a cDNA Library of Vitis pseudoreticulata Native to China Inoculated with Uncinula necator and the Analysis of Potential Defense-related Expressed Sequence Tags

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 993C-993
Author(s):  
Yan Xu ◽  
Yuejin Wang
Keyword(s):  
Cdna Library ◽  
Plant Species ◽  
Expressed Sequence Tags ◽  
Cdna Clones ◽  
Sequence Information ◽  
Uncinula Necator ◽  
The Public ◽  
Perennial Plant ◽  
Vitis Pseudoreticulata ◽  
Expressed Sequence

Expressed sequence tags (ESTs) constitute a rapid and informative strategy for studying gene-expression profiles of specific stages of annual and perennial plant species. Compared with annual plants, the NCBI database has very little sequence information from perennial plant species. To date, only ∼145 ESTs of Vitis pseudoreticulata W.T. Wang have been deposited in databases. This is insufficient to understand the biology and development of this species. In this report, a cDNA library constructed from young leaf inoculated with powdery mildew pathogen [Uncinula necator (Schw.) Burr.] of Chinese wild Vitis pseudoreticulata. Leaf was harvested at various times after inoculation for total RNA extraction, which was used to generate ESTs. In our study, 107 cDNA clones were sequenced either from 5' or 3' end of the cDNAs. Among them, 60 unigenes (56%) were functionally characterized by the BLASTX matches to known function proteins, and 20 unigenes (18.6 %) matched significantly with those having unknown function in the public databases. The remaining 27 unigenes (25.2%) failed to show significant homology to any proteins in the public databases, suggesting that they represent novel sequences. Some functional genes identified from the cDNA library to be potentially associated with plant defence-related responses are discussed.

Cloning of a gar (Lepisosteus osseus) GH cDNA: trends in actinopterygian GH structure

1996 ◽  
Vol 16 (1) ◽  
pp. 73-80 ◽  
Author(s):  
D A Rubin ◽  
J H Youson ◽  
L E Marra ◽  
R M Dores
Keyword(s):  
Cdna Library ◽  
Untranslated Region ◽  
Signal Sequence ◽  
Leader Sequence ◽  
Open Reading Frame ◽  
Russian Sturgeon ◽  
Reading Frame ◽  
Terminal Codon

ABSTRACT A cDNA containing the sequence of GH was cloned and sequenced from a pituitary cDNA library for the holostean fish Lepisosteus osseus (common name: gar). The gar GH cDNA contained an open reading frame of 633 nucleotides and a 3′ untranslated region (including the terminal codon TAG) of 1058 nucleotides. The overall length of the gar GH cDNA including leader sequence, signal sequence, hormone sequence and 3′ untranslated region was 1713 nucleotides. Thus, the gar GH cDNA is the largest vertebrate GH cDNA yet cloned. A comparison of GH sequences from ancient (holostean fishes — gar and bowfin; one chondrostean fish — the Russian sturgeon) and more modern (27 species of teleosts) members of class Actinopterygii indicate that members of this class have maintained many of the invariant residues deemed necessary for GH folding motifs (intramolecular relationships) observed in mammals.

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