scholarly journals Modeling the Effects of Temperature and Gibberellic Acid Concentration on Red Huckleberry Seed Germination

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 223-228 ◽  
Author(s):  
Omar A. Lopez ◽  
Danny L. Barney ◽  
Bahman Shafii ◽  
William J. Price
Keyword(s):  
Logistic Regression ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Temperature Regime ◽  
Potassium Salt ◽  
Logistic Regression Model ◽  
Germination Percentage ◽  
Effects Of Temperature ◽  
Lag Times ◽  
Collection Sites

Low seed germination percentages have been reported for red huckleberry (Vaccinium parvifolium Smith). Attempts to improve germination percentages and the speed of germination for red huckleberry are described. Red huckleberry seeds from two collection sites were given gibberellic acid potassium salt (GA-K) treatments (0, 500, 1000, and 1500 mg·L−1) and were germinated under three temperature regimens [constant 22 °C, 22 °C day/5 °C night (22/5 °C), and 20 °C day/13 °C night (20/13 °C) with a 12-h photoperiod]. A logistic regression model was used to assess the effects of temperature regimens and GA-K treatments on the maximum cumulative germination percentages, rates of increase, and germination lag times. For seeds untreated with GA-K, the 20/13 °C temperature regime resulted in germination percentages ranging from 30% to 61% and lag times (i.e., time to reach one-half of the maximum cumulative germination percentage) of 29 to 35 d for the two accessions. In comparison, the 22/5 °C temperature regime produced germination percentages of 12% and 38% and lag times of 38 to 64 d. The 22 °C constant temperature produced germination percentages ≤1%. Maximum germination percentages of up to 75% were obtained with 1500 mg/L GA-K. Rates of germination were generally unaffected by GA-K treatments, and germination lag times were reduced by an average of 10 d when compared with without GA-K. Improved germination percentages and reduced lag times for red huckleberry seeds were obtained by using a 20/13 °C temperature regime and 1000 to 1500 mg·L −1 GA-K.

scholarly journals Effects of temperature, gibberellic acid, and KNO3 treatments on seed germination of the wild plant Maesa japonica

2020 ◽  
Vol 48 (1) ◽  
pp. 65-72
Author(s):  
Hyuk Joon Kwon ◽  
So Lim Shin ◽  
Yu-Ri Kim ◽  
Soo-Young Kim
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Morphological Characteristics ◽  
Germination Percentage ◽  
Wild Plant ◽  
Optimal Conditions ◽  
Optimum Temperature ◽  
Dark Cycle ◽  
Germination Speed ◽  
Effects Of Temperature

We investigated the morphological characteristics of Maesa japonica seeds and the optimal conditions for germination. The seeds were exalbuminous, and their length and width were 0.85 ± 0.09 mm and 0.77 ± 0.05 mm, respectively. We tested germination at 15, 20, 25, 30 and 35°C; the optimum temperature for germination was 30°C (64% germination). To test the effects of gibberellic acid (GA3) and KNO3 on germination, the seeds were soaked in solutions containing 0, 0.2, 0.5, 1.0, 1.5 or 2.0 g GA3 L-1 or 0, 0.05, 0.10, 0.20 or 0.50 g KNO3 L-1 before sowing for germination. KNO3 treatment increased germination percentage and speed. Soaking in GA3 concentrations over 1.0 g L-1 stimulated 100% germination after 13 days. A presoaking treatment that combined 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 promoted germination speed and shortened the time in which half of the seeds germinated. Overall, we determined that the best method for germination of Maesa japonica seeds was pre-soaking in 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 at 4°C in the dark for 24 hours, followed by incubation at 25–30°C in a 16-hour light, 8-hour dark cycle for 10 days.

scholarly journals Effect of Gibberellic Acid (GA3) and Kinetin on Seed Germination of Sesbania sesban L. and Sesbania rostrata L. (Fabaceae)

2020 ◽  
pp. 32-41
Author(s):  
S. I. Mensah ◽  
C. Ekeke ◽  
N. K. Ibeagi
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Water Absorption ◽  
Glass Container ◽  
Germination Percentage ◽  
International Institute ◽  
Tropical Agriculture ◽  
Percentage Germination ◽  
Seed Coats ◽  
Ibadan Nigeria

We investigated the effects of gibberellic acid (GA3) and kinetin on seed germination of S. sesban L. and S. rostrata L. The matured seeds used for this study were obtained from International Institute for Tropical Agriculture (IITA) Ibadan, Nigeria. The seeds were harvested in 2014 and stored dry in a glass container and kept (15°C) in the refrigerator. The viability of the seeds were determined by floating the intact seeds in water and water uptake (imbibition) was carried out. Four replicates of 20 seeds per replicate were germinated and the seeds observed daily and final count was recorded after 14 days of incubation at 30°C. Intact seeds were soaked in gibberellic acid (GA3) and kinetin for 24h and germination percentage taken. The results from the water absorption demonstrated that the seeds of S. rostrata is more permeable compared to S. sesnban as indicated by higher water absorption of seeds of S. rostrata (70%) to S. sesban (25.4%) after 24hrs of incubation. The anatomy of the seed coats indicated the presence of water and gas impermeable tissues namely cuticle, macrosclereids, osteosclereids and disintegrated parenchyma layer. Generally, 0.1 mM kinetin and GA3 enhanced significant germinations compared to the control with 0% germination for the 14 days period of germination. The percentage germination of seeds of S. sesban and S. rotrata subjected to different treatments and germinated in 0.1 mM GA3 and water showed a progressive decrease in germination. From our study, 0.1 mM GA3 and Kinetin significantly enhanced seed germination of S. sesban and S. rostrata.

scholarly journals Effect of Gibberellic Acid and Indole 3-Acetic Acid on Seed Germination Performance of Horse gram (Macrotyloma uniflorum) Lam (Verdc)

2016 ◽  
Vol 1 (2) ◽  
pp. 36 ◽  
Author(s):  
J. Lalitha ◽  
Haseena Rafath ◽  
M. Subash
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Potential Growth ◽  
Macrotyloma Uniflorum ◽  
Indole 3 Acetic Acid

The experiment was undertaken with an objective to investigate the effect of various concentrations of plant growth regulators, i.e., Gibberellic acid (GA3) and Indole 3-acetic acid (IAA) on seed germination of Macrotyloma uniflorum. Seeds were soaked for 12 hours in different concentrations substances (1.0, 2.0, 3.0 and 4.0 mg/L) of IAA, GA3 and control set was soaked only in distilled water.  Three replicates of each treatment with fifty seeds per replicate were arranged for precise physiological analysis. Significant variation was found in all aspects after analysis of variance (ANOVA) of each mean value. After two weeks of seed soaking, it was noted that germination percentages were significantly accelerated by lower concentrations (1 and 2 mg) of used hormones. Amongst the two potential growth regulators, 2 mg/L was found most effective because it showed highest germination percentage for IAA (93%) and GA3 (88%). A great deal of information relating to seed germination practices shows that these plant growth regulators were efficient in overcoming dormancy leading to rapid seed germination. IAA was selected as best hormone in this study, which showed highest seed germination (93%). 

scholarly journals Sugar apple (Annona squamosa L.) seed germination affected by the application of gibberellins

2016 ◽  
Vol 34 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Fabio Ernesto Martínez M. ◽  
Diego Miranda L. ◽  
Stanislav Magnitskiy
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Positive Response ◽  
Germination Rate ◽  
Germination Percentage ◽  
Annona Squamosa ◽  
Germination Time ◽  
Mean Germination Time ◽  
Germinated Seeds

This research sought to establish the response of the germination percentage (PG), synchrony index (E), mean germination time (MGT) and mean germination rate (MGR) of Annona squamosa L. seeds from Apulo (province of Cundinamarca) and Castilla (province of Tolima), Colombia, to treatments with 0, 50, 100, 200, 400, 600, or 800 mg L-1 of gibberellic acid (GA). All of the treatments with GA increased the PG at each point of time of seed incubation. The 600 mg L-1 GA treatment resulted in higher PGs (92.3% at 16 days for Apulo and 95% at 24 days for Castilla) and lower MGTs (8.75 and 5.38 days for Apulo and Castilla, respectively) than those found with the concentration of 0 mg L-1 GA (17.68 and 10.88 days for Apulo and Castilla, respectively). Also, treating the seeds with 600 mg L-1 GA generated higher MGRs (0.18 and 0.12 germinated seeds/day for Castilla and Apulo, respectively) than those obtained with 0 mg L-1 GA (Castilla = 0.09 and Apulo = 0.06 germinated seeds/ day). Likewise, the germination was synchronized with the application of any concentration of GA. The results evidenced a positive response to the GA application, which provided a tool for the characterization of the phenomenon of dormancy in the A. squamosa seeds.

Effects of temperature, light and stratification on seed germination of Wollemi pine (Wollemia nobilis, Araucariaceae)

2001 ◽  
Vol 49 (6) ◽  
pp. 699 ◽  
Author(s):  
C. A. Offord ◽  
P. F. Meagher
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Constant Temperature ◽  
Field Observations ◽  
Early Autumn ◽  
Adult Trees ◽  
In The Wild ◽  
Effects Of Temperature

Wollemia nobilis W.G.Jones, K.D.Hill D J.M.Allen isrepresented in the wild by about 40 adult trees; therefore, understanding therequirements for seed germination is important for the conservation of thismonotypic Australian conifer. Constant-temperature experiments found that seedgermination proceeded most rapidly at temperatures between 24 and 30˚C.Few seeds germinated when incubated for 112 days at 10 and 16˚C but latergerminated when transferred to 24˚C, whereas seeds initially incubated at35˚C were killed. Unstratified seeds showed a pattern of prolongedgermination taking 130 days to achieve 40% germination at 24˚C and40 days at 27˚C. Seeds stratified at 6˚C for 14 days and incubatedat 27˚C in the light achieved 40% germination within 20 days whilethose incubated at 10 and 16˚C for 112 days and transferred to 24˚Cachieved 40% germination by 15 and 24 days, respectively. Initialgermination of unstratified seeds was fastest when incubated at 30˚C inthe light, averaging 23 days. Exposure to 12-h diurnal periods of 10–15mol m–2 s–1 lightsignificantly increased 28-dayincubation germination percentages, but for onlythose incubated at 30˚C. Additions of gibberellic acid(GA3) at 1 mM had no effect on seed germination ofW. nobilis. The field observations and laboratoryexperiments indicate that following seed shed in summer and early autumn whentemperatures are high, W. nobilis seeds germinate,especially if exposed to light. The seeds that remain ungerminated or that areshed late in the season survive over winter, but germinate rapidly oncetemperatures rise in the next spring.

Can alternating temperature, moist chilling, and gibberellin interchangeably promote the completion of germination in Clematis vitalba seeds?

Botany ◽  
2017 ◽  
Vol 95 (8) ◽  
pp. 847-852 ◽  
Author(s):  
Rosangela Picciau ◽  
Marco Porceddu ◽  
Gianluigi Bacchetta
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Plant Species ◽  
Temperature Regime ◽  
Fluctuating Temperature ◽  
Temperature Regimes ◽  
Moist Chilling ◽  
Clematis Vitalba ◽  
Alternating Temperature ◽  
Positive Effect

Each plant species has particular requirements for seed germination, and some of them respond differently to constant or alternating temperature regimes. In this study, the interchangeable effects of different treatments and temperatures on the completion of seed germination of Clematis vitalba L. were investigated. The seeds were tested with a constant (from 5 °C to 25 °C) or a fluctuating (25/10 °C) temperature regime, and the effect of gibberellic acid (GA3), incubation at warm (W) or cold (C) temperatures while being imbibed, and drying after ripening (DAR) were evaluated. The final germination percentages and the time in days required to reach 50% of germination (T50) were calculated. GA3 and C significantly enhanced completion of seed germination at all of the temperatures tested. A strong positive effect of alternating temperature was observed, which triggered completion of seed germination regardless of treatment. Under the fluctuating temperature, the chilled seeds had the most rapid germination. Low germination rates were observed for both control and DAR treatments. Seeds of C. vitalba display a certain degree of dormancy, which can be broken by moist chilling and GA3 treatments. Moreover, alternating temperature stimulates the completion of seed germination by satisfying certain physiological requirements for germination under constant temperatures.

Seed Germination and storage behavior of Pittosporum eriocarpum Banks. ex Gaertn.: An endemic species of North-West Himalaya

2019 ◽  
Vol 42 (3) ◽  
pp. 239-242
Author(s):  
Bhavana Joshi ◽  
◽  
Giriraj Singh Panwar ◽  
Kumar Ambrish ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seed Viability ◽  
Germination Percentage ◽  
North West ◽  
The North ◽  
West Himalaya ◽  
Physiological Dormancy ◽  
Storage Behavior ◽  
And Storage

Pittosporum eriocarpum Banks. ex Gaertn. (Pittosporaceae) is an endemic and threatened species of the North-West Himalaya and locally known as Agni. In this study the seed viability, seed germination and storage behavior of seeds was studied stored at different temperature. Maximum viability was reported in fresh seeds (98.21%) while the maximum germination percentage (87.11%) was observed in 6-months old seeds stored in refrigerator (polythene). Application of Gibberellic acid has improved the seed germination (54.13%) and to overcome the morpho-physiological dormancy of the seed to an extent.

Response of highbush blueberry seed germination to gibberellin A3 and 6N-benzyladenine

1989 ◽  
Vol 67 (11) ◽  
pp. 3391-3393 ◽  
Author(s):  
I. M. Dweikat ◽  
P. M. Lyrene
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Acid Concentration ◽  
Growth Regulators ◽  
Vaccinium Corymbosum ◽  
Germination Percentage ◽  
Highbush Blueberry ◽  
Gibberellin A3 ◽  
The Relationship ◽  
Germinated Seeds

Open-pollinated highbush blueberry (Vaccinium corymbosum L.) seeds were treated with 0, 2.6, 5.2, 10.4, or 20.8 mM gibberellic acid, 0, 0.4, 2.2, or 4.4 mM 6N-benzyladenine, or the two growth regulators in combination. Seeds were treated for 24 h, then germinated under intermittent mist during summer with temperatures fluctuating between 21 and 40 °C. Germinated seeds were counted weekly from day 15 to day 30, using hypocotyl exposure to indicate germination. Treatment with gibberellic acid at 10.4 mM in combination with 6N-benzyladenine at 0.4 to 2.2 mM gave the highest germination percentage. The relationship between gibberellic acid concentration and seed germination was cubic. Seedlings from the germinated seed flowered and fruited the following spring, making it possible for highbush blueberry to complete a generation in 1 year.

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