scholarly journals Effects of temperature, gibberellic acid, and KNO3 treatments on seed germination of the wild plant Maesa japonica

2020 ◽  
Vol 48 (1) ◽  
pp. 65-72
Author(s):  
Hyuk Joon Kwon ◽  
So Lim Shin ◽  
Yu-Ri Kim ◽  
Soo-Young Kim

We investigated the morphological characteristics of Maesa japonica seeds and the optimal conditions for germination. The seeds were exalbuminous, and their length and width were 0.85 ± 0.09 mm and 0.77 ± 0.05 mm, respectively. We tested germination at 15, 20, 25, 30 and 35°C; the optimum temperature for germination was 30°C (64% germination). To test the effects of gibberellic acid (GA3) and KNO3 on germination, the seeds were soaked in solutions containing 0, 0.2, 0.5, 1.0, 1.5 or 2.0 g GA3 L-1 or 0, 0.05, 0.10, 0.20 or 0.50 g KNO3 L-1 before sowing for germination. KNO3 treatment increased germination percentage and speed. Soaking in GA3 concentrations over 1.0 g L-1 stimulated 100% germination after 13 days. A presoaking treatment that combined 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 promoted germination speed and shortened the time in which half of the seeds germinated. Overall, we determined that the best method for germination of Maesa japonica seeds was pre-soaking in 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 at 4°C in the dark for 24 hours, followed by incubation at 25–30°C in a 16-hour light, 8-hour dark cycle for 10 days.

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 223-228 ◽  
Author(s):  
Omar A. Lopez ◽  
Danny L. Barney ◽  
Bahman Shafii ◽  
William J. Price

Low seed germination percentages have been reported for red huckleberry (Vaccinium parvifolium Smith). Attempts to improve germination percentages and the speed of germination for red huckleberry are described. Red huckleberry seeds from two collection sites were given gibberellic acid potassium salt (GA-K) treatments (0, 500, 1000, and 1500 mg·L−1) and were germinated under three temperature regimens [constant 22 °C, 22 °C day/5 °C night (22/5 °C), and 20 °C day/13 °C night (20/13 °C) with a 12-h photoperiod]. A logistic regression model was used to assess the effects of temperature regimens and GA-K treatments on the maximum cumulative germination percentages, rates of increase, and germination lag times. For seeds untreated with GA-K, the 20/13 °C temperature regime resulted in germination percentages ranging from 30% to 61% and lag times (i.e., time to reach one-half of the maximum cumulative germination percentage) of 29 to 35 d for the two accessions. In comparison, the 22/5 °C temperature regime produced germination percentages of 12% and 38% and lag times of 38 to 64 d. The 22 °C constant temperature produced germination percentages ≤1%. Maximum germination percentages of up to 75% were obtained with 1500 mg/L GA-K. Rates of germination were generally unaffected by GA-K treatments, and germination lag times were reduced by an average of 10 d when compared with without GA-K. Improved germination percentages and reduced lag times for red huckleberry seeds were obtained by using a 20/13 °C temperature regime and 1000 to 1500 mg·L −1 GA-K.


2021 ◽  
Vol 43 ◽  
Author(s):  
Nancy Araceli Godínez-Garrido ◽  
Juan Gabriel Ramírez-Pimentel ◽  
Jorge Covarrubias-Prieto ◽  
Francisco Cervantes-Ortiz ◽  
Artemio Pérez-López ◽  
...  

Abstract: Chitosan is a biopolymer obtained from deacetylation of chitin; it has multiple applications in agriculture as an antifungal, soil conditioner, inducer of defense mechanisms, fruits postharvest coating, leaves and seeds, among others. The objective in this research was to evaluate the effect of chitosan coatings mixed with fungicide (dithiocarbamate) on the germination and germination speed of bean and maize seeds in storage and to determine the retention capacity of the fungicide in the coated seeds under different times of imbibition. Two coating treatments at concentrations of 0.1 and 0.5% chitosan in water, two coatings treatments at 0.1 and 0.5% chitosan supplemented with 0.5% fungicide and a coating without chitosan using only 0.5% fungicide in water were used in bean and maize seed; and as control seeds imbibed in distilled water were used; after treatments, germination percentage and germination speed were determined, also fungicide release were determined at 0, 1, 2 and 6 h of imbibition, and the effect of storage time on germination and germination speed was determined at 30, 60, 90, 120, 150 and 180 days of storage at 4 °C and 45% relative humidity. The fungicide release effect was determined by inhibiting Fusarium oxysporum conidia germination. There were no negative effects of coatings on seed germination after storage. The treatment that provided both greater retention of the fungicidal agent and released it gradually, was 0.5% chitosan mixed with fungicide concentration. Chitosan coating seeds mixed with fungicide do not cause negative changes in seed germination or germination rate.


2011 ◽  
Vol 13 (4) ◽  
pp. 507-511 ◽  
Author(s):  
J.M. Vasconcelos ◽  
M.A. Rodrigues ◽  
S.C. Vasconcelos Filho ◽  
J.F. Sales ◽  
F.G. Silva ◽  
...  

"Quina" (Strychnos pseudoquina A. St. Hil) is a medicinal plant species from the Brazilian Cerrado. As its seeds show dormancy, they were subjected to the treatments pre-cooling at 5ºC during 7 days, pre-heating at 40ºC during 7 days, pre-soaking in sulfuric acid PA during 5 and 15 min, pre-soaking in boiling water during 5 and 15 min, pre-soaking in 100 and 200 ppm gibberellic acid during 48 h, pre-soaking in distilled water during 24 and 48 h, and mechanical scarification to break dormancy. Counts were daily conducted from the 2nd day after the experiment implementation until the germination stabilization at the 65th day. The germination speed index (GSI) and the germination percentage were evaluated. Germination rates above 96% were reached in seeds pre-soaked in water during 48 h and substrate moistened with water or KNO3.


2019 ◽  
Vol 32 (2) ◽  
pp. 411-418
Author(s):  
CLAUDIA KELY PIRES DE MATTOS SORANA ◽  
CARLOS HENRIQUE QUEIROZ REGO ◽  
FERNANDA BRITO CARDOSO ◽  
TIAGO ROQUE BENETOLI DA SILVA ◽  
ANA CARINA DA SILVA CÂNDIDO ◽  
...  

ABSTRACT The aim of the present study was to evaluate the effects of temperature, substrate, and luminosity on chia seeds in order to determine adequate conditions for a germination test. The experiment consisted of a completely randomized design, in a 4 × 5 × 2 factorial scheme (four substrates × five temperatures × two luminosity conditions), with four replications. The following variables were analyzed: first germination count, germination, germination speed index, and mean germination time. Germination was best at 15, 20, 25, and 30 °C for the on-paper, paper roll, and sand substrates. However, in the presence of luminosity and at 25 °C, the on-paper substrate had the highest mean. For all substrates, the lowest germination was achieved at 35 °C, proving that high temperatures reduce chia seed germination. Germination speed index was highest at 25 and 30 °C for on-paper, paper roll, and sand substrates, in both the presence and absence of luminosity. Chia seeds are considered to be neutral photoblasts. Germination tests should be performed with the on-paper substrate, at 25 °C, and with a duration of seven days. The first count should be carried out four days after establishment of the test.


Author(s):  
S. I. Mensah ◽  
C. Ekeke ◽  
N. K. Ibeagi

We investigated the effects of gibberellic acid (GA3) and kinetin on seed germination of S. sesban L. and S. rostrata L. The matured seeds used for this study were obtained from International Institute for Tropical Agriculture (IITA) Ibadan, Nigeria. The seeds were harvested in 2014 and stored dry in a glass container and kept (15°C) in the refrigerator. The viability of the seeds were determined by floating the intact seeds in water and water uptake (imbibition) was carried out. Four replicates of 20 seeds per replicate were germinated and the seeds observed daily and final count was recorded after 14 days of incubation at 30°C. Intact seeds were soaked in gibberellic acid (GA3) and kinetin for 24h and germination percentage taken. The results from the water absorption demonstrated that the seeds of S. rostrata is more permeable compared to S. sesnban as indicated by higher water absorption of seeds of S. rostrata (70%) to S. sesban (25.4%) after 24hrs of incubation. The anatomy of the seed coats indicated the presence of water and gas impermeable tissues namely cuticle, macrosclereids, osteosclereids and disintegrated parenchyma layer. Generally, 0.1 mM kinetin and GA3 enhanced significant germinations compared to the control with 0% germination for the 14 days period of germination. The percentage germination of seeds of S. sesban and S. rotrata subjected to different treatments and germinated in 0.1 mM GA3 and water showed a progressive decrease in germination. From our study, 0.1 mM GA3 and Kinetin significantly enhanced seed germination of S. sesban and S. rostrata.


Author(s):  
V. N. Sorokopudov ◽  

This study is aimed at investigating the impact of the environment temperature and stratification on the Citrus aurantium seed germination. The results show that the optimum environment temperature for the seed germination is found to be 25°C with the maximum percentage of 90%. The stratification of seeds leads to the seed germination percentage decrease by 69% and germination speed increase by 7 days.


CERNE ◽  
2011 ◽  
Vol 17 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Marlene de Matos Malavasi ◽  
Glaucia Bravo Dias ◽  
Ubirajara Contro Malavasi

This work aims to evaluate treatments that may help improve speed and uniformity of germination in fruit tree species tarumã. Data were obtained from pyrenes (stones) of ripe fruits which in turn were picked from adult trees in the municipality of Vera Cruz do Oeste-PR. Treatments consisted of applying GA3 solutions at various concentrations (zero, 50 mg L-1, 100 mg L-1 or 200 mg L-1) to pyrenes via immersion for 47 hours, and thermoperiods of alternate 20ºC and 30ºC (8 hours at 20ºC in light conditions, and 16 hours at 30ºC in dark conditions) and constant 30ºC with the same photoperiod. The combination of alternate temperatures of 20ºC and 30ºC with a 200 mg L-1 GA3 solution resulted in an increase both in germination percentage, from 19.2% to 56.2%, and in the germination speed index, from 0.19 to 3.12, with resulting normal, uniform seedlings.


2016 ◽  
Vol 40 (4) ◽  
pp. 649-660 ◽  
Author(s):  
Márcia Adriana Carvalho dos Santos ◽  
Mailson Monteiro do Rego ◽  
Manoel Abilio de Queiroz ◽  
Bárbara França Dantas ◽  
Wagner Campos Otoni

ABSTRACT The Brazilian guava (Psidium guineense Swartz) is seed-propagated and, being native to the Caatinga biome, may frequently have uneven germination.Thus, we aimed to evaluate the synchronization of the in vitro seed germination of three accessions of the Brazilian guava, using water, polyethyleneglycol (PEG 6000), and potassium nitrate (KNO3) at different potentials and times of osmotic priming. Seeds from three accessions of the Brazilian guava (Y85, Y93,and Y97) from the UNEB/BA Germplasm Active Bank were subjected to the following pretreatments: -0.6, -1.0, -1.4, and -1,8 MPa PEG 6000; 10 and 20% KNO3 for 24h; 10 and 20% KNO3 for 48h; water for 24 and 48h; and non-primed seeds as the control. The experimental design was therefore a 10x3+1 factorial scheme. We assessed the germination percentage (G), mean germination time (MGT), germination speed (GS), and germination speed index (GSI). Data was subjected to analysis of variance followed by a means test (Duncan at 5% probability) and regression. There was interaction between the priming treatments and accessions for all evaluated features, except G. PEG 6000 decreased the MGT (from 6 to 8 days) and increased GS and GSI of seeds from all three accessions at potentials -1.0 to -1.5 MPa.Water-priming had a positive effect on MGT, GS, and GSI of accession Y85 seeds. KNO3 negatively affected germination of seeds from all three accessions. Thereby, we could synchronize seed germination of accessions Y85 and Y97 with PEG 6000.


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