EFFECT OF GIBBERELLIC ACID ON SEED GERMINATION PERCENTAGE AND GROWTH PERFORMANCE OF TWO CASSIA SPECIES

We investigated the effects of gibberellic acid (GA3) and kinetin on seed germination of S. sesban L. and S. rostrata L. The matured seeds used for this study were obtained from International Institute for Tropical Agriculture (IITA) Ibadan, Nigeria. The seeds were harvested in 2014 and stored dry in a glass container and kept (15°C) in the refrigerator. The viability of the seeds were determined by floating the intact seeds in water and water uptake (imbibition) was carried out. Four replicates of 20 seeds per replicate were germinated and the seeds observed daily and final count was recorded after 14 days of incubation at 30°C. Intact seeds were soaked in gibberellic acid (GA3) and kinetin for 24h and germination percentage taken. The results from the water absorption demonstrated that the seeds of S. rostrata is more permeable compared to S. sesnban as indicated by higher water absorption of seeds of S. rostrata (70%) to S. sesban (25.4%) after 24hrs of incubation. The anatomy of the seed coats indicated the presence of water and gas impermeable tissues namely cuticle, macrosclereids, osteosclereids and disintegrated parenchyma layer. Generally, 0.1 mM kinetin and GA3 enhanced significant germinations compared to the control with 0% germination for the 14 days period of germination. The percentage germination of seeds of S. sesban and S. rotrata subjected to different treatments and germinated in 0.1 mM GA3 and water showed a progressive decrease in germination. From our study, 0.1 mM GA3 and Kinetin significantly enhanced seed germination of S. sesban and S. rostrata.

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Effect of Soaking Treatment on Germination of Hard Coated Tropical Forest Tree Seeds

Uganda Journal of Agricultural Sciences ◽

10.4314/ujas.v19i2.1 ◽

2021 ◽

Vol 19 (2) ◽

pp. 1-9

Author(s):

Juventine Boaz Odoi ◽

David Mugeni ◽

Robert Kiiza ◽

Betty Apolot ◽

Samson Gwali

Keyword(s):

Seed Germination ◽

Growth Performance ◽

Seedling Growth ◽

Cold Water ◽

Block Design ◽

Forest Tree ◽

Germination Percentage ◽

Seedling Vigour ◽

Terminalia Catappa ◽

Maesopsis Eminii

Seed germination and seedling growth performance of Maesopsis eminii and Terminalia catappa under different water soaking treatments were evaluated for 120 days under nursery conditions. A total of 1400 seeds were pre-treated with hot (95oC) and cold water (ambient temperature) by soaking for 12, 24 and 48 hours with a control of no soaking. The seeds were sown directly into polythene pots filled with uniform growth medium (top forest soil, sand and clay soilmixed in a ratio of 5:3:2) to avoid disturbance of the root system after germination. The seeds were sown in a randomized block design with seven treatments and three replicates. Data were analysed using ANOVA in GenStat v18. Results indicated that soaking enhanced seed germination. Soaking of seeds in cold water for 12 hours resulted into higher germination (90% for Terminalia catappa and 85% for Maesopsis eminii) than the control (48%). Soaking period and water temperature significantly influenced seedling vigour (F value = 0.962; p = 0.038). Soaking seeds in cold water for 24 hours enhanced Maesopsis eminii seedling growth by 8.0 cm Terminalia catappa seedlings by 7.4 cm. Seed dormancy, germination percentage and growth performance in hard coated seeds such as Maesopsis eminii and Terminalia catappa can be broken by soaking in cold water for 12-24 hours. Pre-germination treatments significantly influences the germination and seedling growth. Keywords: Maesopsis eminii, Terminalia catappa, seeds, pre-germination, soaking.

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Effect of Coconut Water on Pre-Sowing Treatments Additive on Seed Germination and Initial Seedlings Growth Performance of Kamagong (Diospyros discolor)

Asian Journal of Research in Agriculture and Forestry ◽

10.9734/ajraf/2020/v6i430116 ◽

2020 ◽

pp. 58-71

Author(s):

Marne G. Origenes ◽

Renato L. Lapitan

Keyword(s):

Seed Germination ◽

Growth Performance ◽

Germination Percentage ◽

Mean Value ◽

Coconut Water ◽

Leaf Width ◽

Indigenous Species ◽

Initial Growth ◽

Seedling Height ◽

Number Of Leaves

Mabolo (Diospyros discolor), which is an indigenous species locally known as Kamagong, is popularly called “iron-wood”. With over-exploitation, these species are now threatened and are becoming extinct. Hence, it is the only way to conserved and save this economically valuable species by using nursery techniques with appropriate pre-sowing treatments. Therefore, the present study was attempted to evaluate the effect of coconut water on pre-sowing treatments additive on seed germination and initial growth performance of Kamagong (D. discolor). The seeds were extracted and subjected to the following treatments for 12 hours: Soaking in 100% water which is the control (T1), soaking with 50% of coconut water and 50% water (T2), and soaking in 100% coconut water (T3). The results showed that T3 had the highest germination mean value (78.33%), mean seedling height (11.80 cm) and mean number of leaves (2.04). By contrast, T2 (50% coconut water + 50% water) obtained the least germination mean value (76.67%), mean seedling height (11.63 cm) and mean number of leaves (2.00). However, there were no significant differences in the germination percentage, seedling height and number of leaves among all treatments at (p ≤ 0.05). The seeds subjected to T2 and T3 were significantly (p ≤ 0.05) better than seeds subjected to T1, having a mean root collar diameter (RCD) value of 3.98 mm, 3.75 mm and 3.48 mm, respectively. In terms of leaf measurements, the seedlings subjected to T2 and T3 had the highest leaf width value of 5.18 cm and 4.97 cm, respectively, whereas the seedlings subjected to T1 had the significantly lowest leaf width of 4.88 cm. Moreover, there was no significant effect among all treatments in terms of leaf and root length. Furthermore, it was observed in the study that fungal pathogens should also be taken into account as the pathogens are associated with the reduction of the germination percentage of the D. discolor seeds. Also, the study found out that seeds treated with 50% and 100% coconut water positively influenced the germination and initial seedling growth performance of D. discolor.

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Germinability and Seedling Growth Performance of Chilli (Capsicum annuum) Seeds in Response to Different Gibberellic Acid Concentrations Pre-Treatment

AgroTech Food Science, Technology and Environment ◽

10.53797/agrotech.v1i1.2.2021 ◽

2021 ◽

Vol 1 (1) ◽

pp. 10-16

Author(s):

Ryn Brenda Junaidy ◽

Shafeeqa Shahruddin

Keyword(s):

Gibberellic Acid ◽

Growth Performance ◽

Seedling Growth ◽

Crop Production ◽

Germination Rate ◽

Germination Percentage ◽

Dormancy Breaking ◽

Seedling Vigour ◽

Seed Germinability ◽

Significant Difference

Chilli seeds are always associated with physiological dormancy characteristics or endogenous dormancy that has affected their germination potential. Problems noticed on the low seed germination rate, vigour growth, further may lead to the poor seedling growth pattern of chilli crop production. Gibberellic Acid (GA3) which is usually used as a treatment solution is not a new advent of seed dormancy breaking methods for chilli. But, looking forwards to a better GA3 concentration is still needed for the discovery of this treatment potential effect on chilli seed germinability and seedling growth. Thus, this study aimed to evaluate the effect of different GA3 concentrations on seed emergence and seedling growth performance in chilli. In this experiment, chilli seeds were imbibed with 25 mg/L, 75 mg/L and 125 mg/L concentration of GA3 with three replications each; for 24 hours at room temperature (32±4°C) and the untreated seeds as a control. The data collection on final germination percentage was taken daily for 21 days after sowing. While the 15 germinated seeds were directly planted on the 5 polybags arranged in the net house of Junaidy Jonik Farm, Sabah, Malaysia. The experiment was arranged in a completely randomized design with three replications. Data were subjected to analysis of variance with SAS version 9.4 and the significant means were separated by the least significant difference test at P<0.05. Significant differences were observed in the seed germinability measured; germination rate index (GRI), mean germination time (MGT) and final germination percentage (FGP), as well as on seedling vigour index (SVI) between treated and control seeds. Conversely, plant height, number of internodes, number of leaves and fresh weight of seedlings showed no significant differences among treatments. It was concluded that the use of GA3 was able to enhance chilli seeds germinability and could display a better SVI than the control. It is recommended to use GA3 treatment at 25 mg/L of concentration, as it may give an advantage to both economic and biological importance in producing higher germinability and seedling growth performance in chilli.

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Effects of temperature, gibberellic acid, and KNO3 treatments on seed germination of the wild plant Maesa japonica

Seed Science and Technology ◽

10.15258/sst.2020.48.1.09 ◽

2020 ◽

Vol 48 (1) ◽

pp. 65-72

Author(s):

Hyuk Joon Kwon ◽

So Lim Shin ◽

Yu-Ri Kim ◽

Soo-Young Kim

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Morphological Characteristics ◽

Germination Percentage ◽

Wild Plant ◽

Optimal Conditions ◽

Optimum Temperature ◽

Dark Cycle ◽

Germination Speed ◽

Effects Of Temperature

We investigated the morphological characteristics of Maesa japonica seeds and the optimal conditions for germination. The seeds were exalbuminous, and their length and width were 0.85 ± 0.09 mm and 0.77 ± 0.05 mm, respectively. We tested germination at 15, 20, 25, 30 and 35°C; the optimum temperature for germination was 30°C (64% germination). To test the effects of gibberellic acid (GA3) and KNO3 on germination, the seeds were soaked in solutions containing 0, 0.2, 0.5, 1.0, 1.5 or 2.0 g GA3 L-1 or 0, 0.05, 0.10, 0.20 or 0.50 g KNO3 L-1 before sowing for germination. KNO3 treatment increased germination percentage and speed. Soaking in GA3 concentrations over 1.0 g L-1 stimulated 100% germination after 13 days. A presoaking treatment that combined 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 promoted germination speed and shortened the time in which half of the seeds germinated. Overall, we determined that the best method for germination of Maesa japonica seeds was pre-soaking in 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 at 4°C in the dark for 24 hours, followed by incubation at 25–30°C in a 16-hour light, 8-hour dark cycle for 10 days.

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Effect of Gibberellic Acid and Indole 3-Acetic Acid on Seed Germination Performance of Horse gram (Macrotyloma uniflorum) Lam (Verdc)

Journal of Applied and Advanced Research ◽

10.21839/jaar.2016.v1i2.24 ◽

2016 ◽

Vol 1 (2) ◽

pp. 36 ◽

Cited By ~ 2

Author(s):

J. Lalitha ◽

Haseena Rafath ◽

M. Subash

Keyword(s):

Acetic Acid ◽

Seed Germination ◽

Plant Growth ◽

Gibberellic Acid ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Germination Percentage ◽

Potential Growth ◽

Macrotyloma Uniflorum ◽

Indole 3 Acetic Acid

The experiment was undertaken with an objective to investigate the effect of various concentrations of plant growth regulators, i.e., Gibberellic acid (GA3) and Indole 3-acetic acid (IAA) on seed germination of Macrotyloma uniflorum. Seeds were soaked for 12 hours in different concentrations substances (1.0, 2.0, 3.0 and 4.0 mg/L) of IAA, GA3 and control set was soaked only in distilled water. Three replicates of each treatment with fifty seeds per replicate were arranged for precise physiological analysis. Significant variation was found in all aspects after analysis of variance (ANOVA) of each mean value. After two weeks of seed soaking, it was noted that germination percentages were significantly accelerated by lower concentrations (1 and 2 mg) of used hormones. Amongst the two potential growth regulators, 2 mg/L was found most effective because it showed highest germination percentage for IAA (93%) and GA3 (88%). A great deal of information relating to seed germination practices shows that these plant growth regulators were efficient in overcoming dormancy leading to rapid seed germination. IAA was selected as best hormone in this study, which showed highest seed germination (93%).

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Sugar apple (Annona squamosa L.) seed germination affected by the application of gibberellins

Agronomía Colombiana ◽

10.15446/agron.colomb.v33n3.53074 ◽

2016 ◽

Vol 34 (1) ◽

pp. 17-24 ◽

Cited By ~ 2

Author(s):

Fabio Ernesto Martínez M. ◽

Diego Miranda L. ◽

Stanislav Magnitskiy

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Positive Response ◽

Germination Rate ◽

Germination Percentage ◽

Annona Squamosa ◽

Germination Time ◽

Mean Germination Time ◽

Germinated Seeds

This research sought to establish the response of the germination percentage (PG), synchrony index (E), mean germination time (MGT) and mean germination rate (MGR) of Annona squamosa L. seeds from Apulo (province of Cundinamarca) and Castilla (province of Tolima), Colombia, to treatments with 0, 50, 100, 200, 400, 600, or 800 mg L-1 of gibberellic acid (GA). All of the treatments with GA increased the PG at each point of time of seed incubation. The 600 mg L-1 GA treatment resulted in higher PGs (92.3% at 16 days for Apulo and 95% at 24 days for Castilla) and lower MGTs (8.75 and 5.38 days for Apulo and Castilla, respectively) than those found with the concentration of 0 mg L-1 GA (17.68 and 10.88 days for Apulo and Castilla, respectively). Also, treating the seeds with 600 mg L-1 GA generated higher MGRs (0.18 and 0.12 germinated seeds/day for Castilla and Apulo, respectively) than those obtained with 0 mg L-1 GA (Castilla = 0.09 and Apulo = 0.06 germinated seeds/ day). Likewise, the germination was synchronized with the application of any concentration of GA. The results evidenced a positive response to the GA application, which provided a tool for the characterization of the phenomenon of dormancy in the A. squamosa seeds.

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Modeling the Effects of Temperature and Gibberellic Acid Concentration on Red Huckleberry Seed Germination

HortScience ◽

10.21273/hortsci.43.1.223 ◽

2008 ◽

Vol 43 (1) ◽

pp. 223-228 ◽

Cited By ~ 2

Author(s):

Omar A. Lopez ◽

Danny L. Barney ◽

Bahman Shafii ◽

William J. Price

Keyword(s):

Logistic Regression ◽

Seed Germination ◽

Gibberellic Acid ◽

Temperature Regime ◽

Potassium Salt ◽

Logistic Regression Model ◽

Germination Percentage ◽

Effects Of Temperature ◽

Lag Times ◽

Collection Sites

Low seed germination percentages have been reported for red huckleberry (Vaccinium parvifolium Smith). Attempts to improve germination percentages and the speed of germination for red huckleberry are described. Red huckleberry seeds from two collection sites were given gibberellic acid potassium salt (GA-K) treatments (0, 500, 1000, and 1500 mg·L−1) and were germinated under three temperature regimens [constant 22 °C, 22 °C day/5 °C night (22/5 °C), and 20 °C day/13 °C night (20/13 °C) with a 12-h photoperiod]. A logistic regression model was used to assess the effects of temperature regimens and GA-K treatments on the maximum cumulative germination percentages, rates of increase, and germination lag times. For seeds untreated with GA-K, the 20/13 °C temperature regime resulted in germination percentages ranging from 30% to 61% and lag times (i.e., time to reach one-half of the maximum cumulative germination percentage) of 29 to 35 d for the two accessions. In comparison, the 22/5 °C temperature regime produced germination percentages of 12% and 38% and lag times of 38 to 64 d. The 22 °C constant temperature produced germination percentages ≤1%. Maximum germination percentages of up to 75% were obtained with 1500 mg/L GA-K. Rates of germination were generally unaffected by GA-K treatments, and germination lag times were reduced by an average of 10 d when compared with without GA-K. Improved germination percentages and reduced lag times for red huckleberry seeds were obtained by using a 20/13 °C temperature regime and 1000 to 1500 mg·L −1 GA-K.

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Seed Germination and storage behavior of Pittosporum eriocarpum Banks. ex Gaertn.: An endemic species of North-West Himalaya

Indian Journal of Forestry ◽

10.54207/bsmps1000-2019-3a3kj3 ◽

2019 ◽

Vol 42 (3) ◽

pp. 239-242

Author(s):

Bhavana Joshi ◽

◽

Giriraj Singh Panwar ◽

Kumar Ambrish ◽

◽

...

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Seed Viability ◽

Germination Percentage ◽

North West ◽

The North ◽

West Himalaya ◽

Physiological Dormancy ◽

Storage Behavior ◽

And Storage

Pittosporum eriocarpum Banks. ex Gaertn. (Pittosporaceae) is an endemic and threatened species of the North-West Himalaya and locally known as Agni. In this study the seed viability, seed germination and storage behavior of seeds was studied stored at different temperature. Maximum viability was reported in fresh seeds (98.21%) while the maximum germination percentage (87.11%) was observed in 6-months old seeds stored in refrigerator (polythene). Application of Gibberellic acid has improved the seed germination (54.13%) and to overcome the morpho-physiological dormancy of the seed to an extent.

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