New Breeding Lines Resistant to Tomato Mosaic Virus and Tomato Spotted Wilt Virus within the ‘De la Pera’ Tomato Type: UMH 1353 and UMH 1354

Abstract A protocol for the reverse transcription-helicase-dependent amplification (RT–HDA) of isothermal DNA was developed for the detection of tomato spotted wilt virus (TSWV). Specific primers, which were based on the highly conserved region of the N gene sequence in TSWV, were used for the amplification of virus's RNA. The LOD of RT–HDA, reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP), and reverse transcriptase-polymerase chain reaction (RT-PCR) assays were conducted using 10-fold serial dilution of RNA eluates. TSWV sensitivity in RT–HDA and RT-LAMP was 4 pg RNA compared with 40 pg RNA in RT-PCR. The specificity of RT–HDA for TSWV was high, showing no cross-reactivity with other tomato and Tospovirus viruses including cucumber mosaic virus (CMV), tomato black ring virus (TBRV), tomato mosaic virus (ToMV), or impatiens necrotic spot virus (INSV). The RT–HDA method is effective for the detection of TSWV in plant samples and is a potential tool for early and rapid detection of TSWV.

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Incidence of viruses infecting pepper in Thailand

BioMolecular Concepts ◽

10.1515/bmc-2019-0021 ◽

2019 ◽

Vol 10 (1) ◽

pp. 184-193

Author(s):

Amisa Laprom ◽

Somrudee Nilthong ◽

Ekachai Chukeatirote

Keyword(s):

Mosaic Virus ◽

Mixed Infection ◽

Tomato Spotted Wilt Virus ◽

Potato Virus Y ◽

Enzyme Linked Immunosorbent Assay ◽

Mixed Infections ◽

The North ◽

Tomato Spotted Wilt ◽

Wilt Virus ◽

Chilli Veinal Mottle Virus

AbstractThis study was conducted to determine the incidence, diversity and distribution of viruses infecting pepper (Capsicum spp.) in the central, northern and northeastern parts of Thailand. During a survey in 2016 - 2019, a total of 2,149 leaf samples from symptomatic and asymptomatic peppers were collected randomly from farmer’s fields, and preliminary tested by an enzyme-linked immunosorbent assay (ELISA) using 7 antibodies specific for cucumber mosaic virus (CMV), chilli veinal mottle virus (ChiVMV), tomato necrotic ringspot virus (TNRV), tobacco mosaic virus (TMV), potato virus Y (PVY), tomato spotted wilt virus (TSWV), and begomoviruses. Our data revealed that the incidence of the viruses infecting pepper in Thailand was high, accounting for nearly 70% (1,482 infected samples). The highest viral incidence was found in the central part (96%), followed by the north (74.4%) and the northeastern (52.8%), respectively. Begomoviruses, CMV, ChiVMV, and TNRV were detected in the samples at varying rates, whereas PVY, TMV, and TSWV were not detected. Of these, the most frequently found virus was Begomoviruses accounting for nearly 33%, with the highest rate (ca. 82%) in the central Provinces of Thailand. In addition, of the 1,482 infected samples, mixed infections among the four viruses were also found in 616 samples (ca. 42%), and CMV + ChiVMV (approximately 11%) was the most common mixed infection. This is the first report describing an occurrence of viruses in pepper of Thailand, and the results obtained have revealed that viruses infecting pepper are widespread, which may pose a threat to pepper production in Thailand.

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Existence of Antiviral Principles (AVPs) in Seed Extracts of Harpullia cupanioides (Roxb.) against Tomato Spotted Wilt Virus (TSWV), Rice Tungro Virus (RTV) and Cowpea Aphid Borne Mosaic Virus (CABMV)

Acta Phytopathologica et Entomologica Hungarica ◽

10.1556/aphyt.38.2003.1-2.13 ◽

2003 ◽

Vol 38 (1-2) ◽

pp. 109-114 ◽

Cited By ~ 5

Author(s):

C. Bharathimatha ◽

Sabitha Doraiswamy ◽

R. Rabindran ◽

P. Renukadevi ◽

R. Velazhahan

Keyword(s):

Mosaic Virus ◽

Tomato Spotted Wilt Virus ◽

Tomato Spotted Wilt ◽

Cowpea Aphid ◽

Wilt Virus ◽

Seed Extracts

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Bakterijske i virusne bolesti rajčice i paprike u Hrvatskoj

Glasnik zaštite bilja ◽

10.31727/gzb.42.3.6 ◽

2019 ◽

Vol 42 (3) ◽

pp. 36-42

Author(s):

Dario Ivić

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Pseudomonas Syringae ◽

Tomato Spotted Wilt Virus ◽

Clavibacter Michiganensis ◽

Tomato Spotted Wilt ◽

Wilt Virus

U proteklih nekoliko godina u Hrvatskoj su zabilježene relativno visoke štete od pojedinih bakterijskih i virusnih bolesti u proizvodnji rajčice i paprike. Uzročnici tih bolesti bile su bakterije Pseudomonas syringae pv. tomato i Clavibacter michiganensis ssp. michiganensis te virusi Tomato spotted wilt virus (TSWV) i Cucumber mosaic virus (CMV). U članku su ukratko opisani spomenuti patogeni, njihovo širenje te mjere zaštite.

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High Levels of Field Resistance to Tomato spotted wilt virus in Peanut Breeding Lines Derived from hypogaea and hirsuta Botanical Varieties

Peanut Science ◽

10.3146/0095-3679(2005)32[20:hlofrt]2.0.co;2 ◽

2005 ◽

Vol 32 (1) ◽

pp. 20-24 ◽

Cited By ~ 31

Author(s):

A. K. Culbreath ◽

D. W. Gorbet ◽

N. Martinez-Ochoa ◽

C. C. Holbrook ◽

J. W. Todd ◽

...

Keyword(s):

Tomato Spotted Wilt Virus ◽

Field Resistance ◽

Breeding Lines ◽

Tomato Spotted Wilt ◽

Wilt Virus ◽

Botanical Varieties

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BREEDING TRANSGENIC TOMATOES FOR RESISTANCE TO TOMATO SPOTTED WILT VIRUS AND CUCUMBER MOSAIC VIRUS

Acta Horticulturae ◽

10.17660/actahortic.1996.431.40 ◽

1996 ◽

pp. 442-448 ◽

Cited By ~ 5

Author(s):

C. Gonsalves ◽

B. Xue ◽

S.Z. Pang ◽

R. Provvidenti ◽

J.L. Slightom ◽

...

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Tomato Spotted Wilt Virus ◽

Tomato Spotted Wilt ◽

Transgenic Tomatoes ◽

Wilt Virus

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Variation Among Virginia Market-Type Peanut Genotypes in Susceptibility to Tomato Spotted Wilt Virus Vectored by Thrips (Thysanoptera: Thripidae)

Peanut Science ◽

10.3146/ps07-105.1 ◽

2008 ◽

Vol 35 (2) ◽

pp. 92-100 ◽

Cited By ~ 1

Author(s):

S. D. Riniker ◽

R. L. Brandenburg ◽

G. G. Kennedy ◽

T. G. Isleib ◽

D. L. Jordan

Keyword(s):

North Carolina ◽

Tomato Spotted Wilt Virus ◽

Field Tests ◽

Breeding Line ◽

Breeding Lines ◽

Tomato Spotted Wilt ◽

Foliar Symptoms ◽

Arachis Hypogaea L ◽

Highly Correlated ◽

Wilt Virus

Abstract Tomato spotted wilt virus (TSWV), a thrips-vectored tospovirus, is an important pathogen of peanut (Arachis hypogaea L.). Development of tolerant cultivars has proven to be one of the most promising methods to manage the disease. Twenty-four genotypes of virginia market-type peanut were monitored in field tests for thrips damage, and TSWV incidence and severity during 2004 and 2005 in North Carolina. The cultivar Gregory had a higher density of adult thrips in foliage than any other genotype, while breeding lines N01057 and N03054E had the lowest density. No significant correlation was detected between thrips density or injury and TSWV incidence. Line N03036EJ had the greatest TSWV incidence, but did not differ from cultivars Gregory or Perry in incidence. Line N00033 had the least TSWV incidence and differed from the cultivars Gregory and Perry. The occurrence of late-season chlorosis or peanut yellowing death (PYD) was highly correlated with TSWV infection (P < 0.0001). Breeding line N02051ol had the greatest incidence of PYD, but did not differ statistically from Gregory or Perry. Lines N03023EF and N01083 had the least PYD incidence. Plants infected with TSWV not expressing foliar symptoms were found in far greater abundance than plants that were infected and symptomatic. Line N03036EJ had the greatest proportion of infected but asymptomatic plants; line N03054E had the least. Susceptible lines are more likely to become infected, rather than just more likely to show spotted wilt symptoms.

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Phenotypic and Molecular Evaluation of Interspecific Peanut (Arachis) Lines

Peanut Science ◽

10.3146/pnut.31.2.0001 ◽

2004 ◽

Vol 31 (2) ◽

pp. 65-70 ◽

Cited By ~ 3

Author(s):

W. F. Anderson ◽

G. Kochert ◽

C. C. Holbrook ◽

H. T. Stalker

Keyword(s):

Wild Species ◽

Leaf Spot ◽

Tomato Spotted Wilt Virus ◽

Plant Morphology ◽

Interspecific Crosses ◽

Root Knot Nematode ◽

Breeding Lines ◽

Resistant Disease ◽

Tomato Spotted Wilt ◽

Wilt Virus

Abstract Peanut breeders are constantly in search of new sources of genes that confer tolerance or resistance to biotic and abiotic stresses to improve the production and quality. The objective of this study was to evaluate peanut lines generated from interspecific crosses for amounts of wild species introgression, including genes for resistance to peanut root-knot nematodes, tomato spotted wilt virus and leaf spot diseases. Nine diploid Arachis species were crossed with peanut breeding lines and 130 different interspecific hybrid lines were developed. These lines were evaluated for the amount of introgression using RFLP analyses, plant morphology, and disease resistant phenotypes. Based on RFLPs, 41 lines showed measurable introgression and 12 hexaploid-derived lines were polymorphic for at least four probes. Greenhouse and field evaluations indicated that resistance was not present in the lines tested for tomato spotted wilt virus, early leaf spot, or Cylindrocladium black rot. However, resistance approaching that of the wild species was found for the peanut root-knot nematode (Meloidogyne arenaria) among lines derived from crosses with Arachis diogoi, A. correntina, A. batizocoi, and A. cardenasii. Introgression lines were resistant (disease ratings of 1.5 to 4.5 and lesion numbers 8 to 63) compared to Southern Runner (ratings of 5.5 to 6 and lesion numbers of nearly 500) for late leaf spot (Cercosporidium personation) in field evaluations performed in Gainesville, FL over 2 yr. The greatest resistance was found among lines from crosses with A. batizocoi, A. duranensis, A. stenosperma, A. magma, and A. diogoi. Results indicate that it should be possible to identify molecular markers to tag resistance genes for use in conventional breeding programs and stack these genes in highly productive peanut cultivars.

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