scholarly journals Comparison of Cold Hardiness Evaluation of Woody Species by ELLT and TTCLT

HortScience ◽  
2020 ◽  
Vol 55 (8) ◽  
pp. 1228-1232
Author(s):  
Hui-qing Li ◽  
Qing-he Li ◽  
Lei Xing ◽  
Gao-jie Sun ◽  
Xiu-lian Zhao
Keyword(s):  
Electrolyte Leakage ◽  
Cold Hardiness ◽  
Woody Species ◽  
Stem Tissue ◽  
Lethal Temperature ◽  
Triphenyltetrazolium Chloride ◽  
Extreme Minimum Temperature ◽  
Humidity Chamber ◽  
One Year ◽  
Dead Tissue

Cold hardiness evaluation is important for screening woody species in cold areas. We compared cold hardiness by estimating the 50% lethal temperature (LT50) using electrolyte leakage test (ELLT50) and triphenyltetrazolium chloride test (TTCLT50) for 26 woody species in the Bashang region of China. One-year-old shoots were collected in January and exposed to five subfreezing temperatures in a programmable temperature and humidity chamber. LT50 was estimated by fitting relative electrolyte leakage and percentage of dead tissue against test temperature. For all tested species, triphenyltetrazolium chloride (TTC) staining of the pith was weak and the cambium TTCLT50 was lower than the extreme minimum temperature (−37 °C) recorded in the region. The cambium TTCLT50 and the sd were lower than that for the phloem and xylem. The phloem TTCLT50 was lower than the xylem TTCLT50, and the two sds were similar. The ELLT50 showed no significant correlation with any TTCLT50. For most species, the ELLT50 was higher than the cambium and phloem TTCLT50 and was not significant different with the xylem TTCLT50. The ELLT50 showed higher sd than any tissue TTCLT50. Based on results obtained in this study, when choosing cold hardiness of single stem tissue as an indicator for screening woody species, the xylem should be considered first, followed by the phloem; the cambium and pith were unsuitable. The cold hardiness estimated by ELLT50 was more suitable as indicator for screening woody species than that of stem tissue in winter estimated by TTCLT50.

Thaw effects on cold-hardiness parameters in yellow birch

2002 ◽  
Vol 80 (4) ◽  
pp. 390-398 ◽  
Author(s):  
X B Zhu ◽  
R M Cox ◽  
C -PA Bourque ◽  
P A Arp
Keyword(s):  
Climate Change ◽  
Electrolyte Leakage ◽  
Cold Hardiness ◽  
Freezing Injury ◽  
Climate Change Scenarios ◽  
Betula Alleghaniensis ◽  
Yellow Birch ◽  
Triphenyltetrazolium Chloride ◽  
Root Pressure ◽  
Cold Temperatures

One-year-old, cold-hardened, container-grown yellow birch (Betula alleghaniensis Britt.) seedlings were exposed to cold treatments after being pretreated with a simulated winter thaw. Freezing injury to roots and shoots was assessed by relative electrolyte leakage and triphenyltetrazolium chloride reduction. Growth characteristics were also determined after 60 days under greenhouse conditions. Relative electrolyte leakage and triphenyltetrazolium chloride reduction measurements showed that roots became increasingly damaged with decreasing cold-treatment temperatures. However, plants pretreated with thaws showed significantly lower stem increment, shoot length, and leaf area in response to the cold temperatures than did the unthawed plants. Variation in these growth parameters was also significantly correlated with both root and shoot freezing injury parameters. Cold hardiness under different thaw pretreatments was assessed using the highest freezing temperature that caused significant injury, referred to as the critical temperature. For seedlings without the thaw pretreatment, shoot and root critical temperatures were estimated as –52.5 and 23.8°C, respectively. Following 12 days of thaw, these temperatures increased to –24.08°C for shoots and –13°C for roots. Twelve days of thaw, or growing degree-day (>4°C) accumulations greater than 66 during a thaw, could sufficiently deharden roots and shoots such that they would be susceptible to freezing damage at ambient temperatures commonly encountered in the Canadian Maritimes. We also observed that root pressure declined significantly with increasing root freezing injury. Sufficient root pressure is required for springtime refilling of xylem embolisms caused by winter cavitation of the vessels in this species. Weak root pressure caused by freezing injury would represent a risk of shoot dieback and tree decline due to the remaining embolisms reducing water flow to the developing foliage. The rapid reduction of shoot cold hardiness may also indicate the threat of late-spring frosts to this species. These induced changes are especially important under climate change scenarios that suggest increases in winter temperatures and changes in seasonality in eastern Canada.Key words: climate change, cold hardiness, electrolyte leakage, growth, root pressure, TTC reduction.

Seasonal and geographic origin effects on cold hardiness of white spruce buds, foliage, and stems

1994 ◽  
Vol 24 (5) ◽  
pp. 1066-1070 ◽  
Author(s):  
David G. Simpson
Keyword(s):  
Thermal Analysis ◽  
Differential Thermal Analysis ◽  
Low Temperature ◽  
North American ◽  
Electrolyte Leakage ◽  
Cold Hardiness ◽  
Geographic Origin ◽  
White Spruce ◽  
Stem Tissue ◽  
Early Fall

Cold hardiness of dormant vegetative buds from white spruce (Piceaglauca (Moench) Voss) trees originating from throughout the species' North American range was measured by differential thermal analysis between August and April at Vernon, B.C. Buds were least hardy in early fall (low temperature exotherm −12 to −25 °C) and most hardy midwinter (low temperature exotherms −20 to −34 °C). Buds of northerly origin trees were more hardy in early fall (September–October) than buds of more southerly origin trees. Similar results were observed for foliage and stem tissue in early fall using electrolyte leakage measurements following −16 °C freezing.

scholarly journals Relating Freeze-induced Electrolyte Leakage Measurements to Lethal Temperature in Red Raspberry

2000 ◽  
Vol 125 (4) ◽  
pp. 429-435 ◽  
Author(s):  
Leena Lindén ◽  
Pauliina Palonen ◽  
Mikael Lindén
Keyword(s):  
Electrolyte Leakage ◽  
Cold Hardiness ◽  
Rubus Idaeus ◽  
Data Sets ◽  
Red Raspberry ◽  
Lethal Temperature ◽  
Gompertz Function ◽  
Visual Rating ◽  
Richards Function ◽  
Response Percentage

Seasonal cold hardiness of red raspberry (Rubus idaeus L.) canes was measured by freeze-induced electrolyte leakage test and visual rating of injury. Leakage data were transformed to percentage-adjusted injury values and related to lethal temperature by graphical interpolation and by the midpoint (T50) and inflection point (Tmax) estimates derived from three sigmoid (the logistic, Richards, and Gompertz) functions. Tmax estimates produced by Richards and Gompertz functions were corrected further using two different procedures. The 10 leakage-based hardiness indices, thus derived, were compared to lethal-temperature estimates based on visual rating. Graphical interpolation and Tmax of the logistic or T50 of the Gompertz function yielded lethal-temperature estimates closest to those obtained visually. Also, Tmax values of the Gompertz function were well correlated with visual hardiness indices. The Richards function yielded hardiness estimates deviating largely from visual rating. In addition, the Richards function displayed a considerable lack of fit in several data sets. The Gompertz function was preferred to the logistic one as it allows for asymmetry in leakage response. Percentage-adjusted injury data transformation facilitated curve-fitting and enabled calculation of T50 estimates.

COLD HARDENING AND DEHARDENING RESPONSES IN WINTER WHEAT AND WINTER BARLEY

1975 ◽  
Vol 55 (2) ◽  
pp. 529-535 ◽  
Author(s):  
M. K. POMEROY ◽  
C. J. ANDREWS ◽  
G. FEDAK
Keyword(s):  
Winter Wheat ◽  
Cold Hardiness ◽  
Maximum Level ◽  
Freezing Temperature ◽  
Triticum Aestivum L ◽  
Winter Barley ◽  
Lethal Temperature ◽  
Hordeum Vulgare L ◽  
Wheat Seedlings ◽  
Time Required

Increasing the duration of freezing of Kharkov winter wheat (Triticum aestivum L.) demonstrated that severe injury does not occur to plants at a freezing temperature (−6 C) well above the lethal temperature for at least 5 days, but progressively more damage occurs as the temperature approaches the killing point (−20 C). High levels of cold hardiness can be induced rapidly in Kharkov winter wheat if seedlings are grown for 4–6 days at 15 C day/10 C night, prior to being exposed to hardening conditions including diurnal freezing to −2 C. The cold hardiness of Kharkov and Rideau winter wheat seedlings grown from 1-yr-old seed was greater than that from 5-yr-old seed. Cold-acclimated Kharkov winter wheat and Dover winter barley (Hordeum vulgare L.) demonstrated the capacity to reharden after varying periods under dehardening conditions. The time required to reharden and the maximum level of hardiness attained by the plants was dependent on the amount of dehardening. Considerable rehardening was observed even when both dehardening and rehardening were carried out in the dark.

scholarly journals Woody species do not differ in dormancy progression: differences in time to budbreak due to forcing and cold hardiness

2021 ◽  
Author(s):  
Al Kovaleski
Keyword(s):  
Maximum Rate ◽  
Path Length ◽  
Cold Hardiness ◽  
Woody Species ◽  
Two Dimensions ◽  
Spring Phenology ◽  
Wide Range ◽  
The Difference ◽  
Response To Temperature ◽  
Phenological Phases

AbstractBudbreak is one of the most observed and studied phenological phases in perennial plants. Two dimensions of exposure to temperature are generally used to model budbreak: accumulation of time spent at low temperatures (chilling); and accumulation of heat units (forcing). These two effects have a well-established negative correlation: the more chilling, the less forcing required for budbreak. Furthermore, temperate plant species are assumed to vary in amount of chilling required to complete endodormancy and begin the transition to breaking bud. Still, prediction of budbreak remains a challenge. The present work demonstrates across a wide range of species how bud cold hardiness must be accounted for to study dormancy and accurately predict time to budbreak. Cold hardiness defines the path length to budbreak, meaning the difference between the cold hardiness buds attain during the winter, and the cold hardiness at which deacclimated buds are predicted to open. This distance varies among species and throughout winter within a species. Increases in rate of cold hardiness loss (deacclimation) measured throughout winter show that chilling controls deacclimation potential – the proportion of the maximum rate response attained at high chill accumulation – which has a sigmoid relationship to chilling accumulation. For forcing, rates of deacclimation increase non-linearly in response to temperature. Comparisons of deacclimation potential show a dormancy progresses similarly for all species. This observation suggests that comparisons of physiologic and genetic control of dormancy requires an understanding of cold hardiness dynamics and the necessity for an update of the framework for studying dormancy and its effects on spring phenology.

scholarly journals Slow-release fertilizers and possibilities of their utilizing in nursery

2004 ◽  
Vol 52 (2) ◽  
pp. 155-164
Author(s):  
Petr Salaš
Keyword(s):  
Slow Release ◽  
Ornamental Plants ◽  
Woody Species ◽  
Soil Surface ◽  
Berberis Thunbergii ◽  
Slow Release Fertilizers ◽  
Picea Omorika ◽  
One Year ◽  
Two Stages

Reserve, slow-release fertilizers (SRF) enable to simplify the whole system of plant nutrition and fertilisation. Tabletted fertilizers of the Silvamix series represent a prospective product of Czech provenience. At our university, these fertilizers have been tested and used since the year 1991. Ornamental woody species grown in containers were investigated in two stages. Experiments with ornamental plants were established using one-year-old cuttings and seedlings of the following deciduous and evergreen woody species:Cotoneaster dammeri Skogholm,Berberis thunbergii,Potentilla fruticosa Snowflake,Ligustrum vulgare AtrovirensandPicea omorika. After planting into containers, fertilizers in the dose of 1 tablet (i.e. 10 g) per litre of substrate were applied either to roots level or on the soil surface in the container. Silvamix in the dose of 5 g.l-1was used as the tested fertilizer in the second stage. It was applied during the planting in the form of tablets and/or a powder. Control plants were fertilized in the course of growing season using a common agricultural fertilizer Cererit Z. The annual plants increments were measured. These experiments demonstrated a long-term optimum effect of this product on woody species and an equal quality and efficiency of its tabletted and powdered forms.

Fructan in winter wheat, triticale, and fall rye cultivars of varying cold hardiness

1988 ◽  
Vol 66 (9) ◽  
pp. 1723-1728 ◽  
Author(s):  
Michio Suzuki ◽  
H. G. Nass
Keyword(s):  
Winter Wheat ◽  
Cold Hardiness ◽  
Wheat Cultivar ◽  
Degree Of Polymerization ◽  
Lethal Temperature ◽  
Winter Wheat Cultivar ◽  
Freezing Resistance ◽  
Winter Cereals ◽  
Opposite Trend ◽  
High Concentrations

Eight winter wheat, one triticale, and three fall rye cultivars with mean lethal temperature (LT50) values from −5.5 to −20.0 °C were harvested in late November and analyzed for fructans. Fructose, sucrose, and oligofructans with a degree of polymerization (DP) of 6 or lower were found in all cultivars. The concentration of DP 4 fructan was higher than that of DP 5 in winter wheat and triticale, while the opposite trend was found in fall rye. Fructans with a DP of 7 or higher (high DP fructans) were found at high concentrations in hardy winter wheat and fall rye. The high DP fructan was very low or negligible in the least hardy winter wheat cultivar 'Super X'. Fructans in winter cereals consisted mainly of inulin type with a β-2-1 linkage. The activity of phlein sucrase, which catalyzes synthesis of phlein, was much lower in winter cereals compared with phlein-rich grasses. It was concluded that high DP fructans of inulin type in basal top tissues of winter cereals were more closely associated with freezing resistance than low DP fructans.

scholarly journals Changes in cold hardiness of silver fir and larch bare-rooted seedlings during autumn and spring

2012 ◽  
Vol 50 (No. 5) ◽  
pp. 237-242 ◽  
Author(s):  
M. Sarvaš
Keyword(s):  
Root System ◽  
Electrolyte Leakage ◽  
Untreated Control ◽  
Cold Hardiness ◽  
Silver Fir ◽  
Clear Tendency ◽  
Control Root

The objective of this study was to obtain information about changes in cold hardiness of larch and silver fir seedlings during autumn and spring by help of measurements of electrolyte leakage from shoots (SEL) and root system (REL). The values of electrolyte leakage from the untreated (control) root system of silver fir decreased during autumn (from 28% on September 25 to 24% on November 27). Minimum values were reached on March 26. A decrease in electrolyte leakage was found for silver fir shoots (SEL) (the maximum was detected on October 2 – 12% and minimum on November 27 – 7%). Contrary to REL, SEL increased in March. The rate of electrolyte leakage from treated (after artificial frost) roots and shoots decreased during autumn (REL and SEL minimum on November 27). The change in the rate of electrolyte leakage from untreated larch roots was similar to that from silver fir roots during autumn. The values continually decreased from 26% (on September 25) to 12% (on November 27). The course of electrolyte leakage from the treated root system was similar for both species. The differences between electrolyte leakage from larch shoots (treated und untreated ones) were statistically significant, but without any clear tendency during autumn.

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