scholarly journals Etiolation Improves Rooting of Bigtooth Maple Cuttings

2012 ◽  
Vol 22 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Melody Reed Richards ◽  
Larry A. Rupp
Keyword(s):  
Butyric Acid ◽  
Naphthaleneacetic Acid ◽  
Small Water ◽  
Acer Grandidentatum ◽  
Bigtooth Maple ◽  
Wild Accessions

Bigtooth maple (Acer grandidentatum) has potential as a small, water conserving landscape tree in western landscapes. This potential has been hindered in part by the difficulty in asexually propagating superior accessions. The ability of etiolation to enhance rooting of softwood cuttings of selected wild accessions was determined by grafting six accessions onto seedling rootstocks to use as stock plants. Etiolation was applied to stock plants by placing open-ended, black, velour, drawstring bags over the end of pruned shoots at bud swell allowing new shoots to develop and grow out the end of the bag while leaving the base of the shoot covered. In 2009 and 2010, cuttings from etiolated and nonetiolated shoots were treated with 4000 ppm indole-3-butyric acid (IBA) + 2000 ppm naphthaleneacetic acid (NAA), stuck in a premoistened 3 perlite:1 peat (by volume) rooting substrate and placed under intermittent mist. After 4 weeks, 89% (2009) and 85% (2010) of the etiolated cuttings rooted and only 47% (2009) and 17% (2010) of the nonetiolated cuttings rooted. Etiolated cuttings produced on average 11.3 (2009) and 7.2 (2010) roots per cutting and nonetiolated 2.1 (2009) and 0.5 (2010) roots per cutting. Etiolation, and its application through the use of black cloth bags, can be an effective way to increase the rooting of bigtooth maple cuttings and the availability of these plants for use in water conserving landscaping.

scholarly journals Selection and Budding Propagation of Native Bigtooth Maple for Water-conserving Landscapes

2012 ◽  
Vol 22 (5) ◽  
pp. 669-676 ◽  
Author(s):  
Melody Reed Richards ◽  
Larry A. Rupp ◽  
Roger Kjelgren ◽  
V. Philip Rasmussen
Keyword(s):  
Time Course ◽  
Wild Plants ◽  
Small Water ◽  
Acer Grandidentatum ◽  
Global Positioning ◽  
Northern Utah ◽  
Bigtooth Maple ◽  
Plant Form ◽  
Wild Accessions ◽  
Selection Of

The potential of bigtooth maple (Acer grandidentatum) as a small, water-conserving landscape tree for the western United States is limited by the selection of superior accessions from a morphologically diverse gene pool and the ability to propagate wild plants in a nursery environment. Superior accessions were selected based primarily on red fall color. Aerial digital images taken during peak fall color in 2007 and 2008 were synchronized with flight global positioning system (GPS) track files using digital image editor software and visually compared with corresponding satellite images to determine the exact latitude and longitude of selected trees on the ground. Trees were physically located using GPS technology then visually evaluated for initial selection. Criteria included fall color, habitat, relative disease and insect resistance, bud quality, and plant form. From 56 observed trees of interest, six were selected for propagation. Through time-course experiments using multistemmed, bigtooth maple seedling rootstocks in a coppiced stoolbed, the optimum time for chip budding scions of wild accessions in northern Utah was determined to be July through mid-August. Further evaluation of accessions for use in the landscape industry is required.

scholarly journals Rooting of Rose Cuttings in Response to Foliar Applications of Auxin and Surfactant

2004 ◽  
Vol 14 (4) ◽  
pp. 479-483 ◽  
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
Ken M. Tilt ◽  
John M. Ruter
Keyword(s):  
Butyric Acid ◽  
Root Length ◽  
Potassium Salt ◽  
Foliar Application ◽  
Foliar Spray ◽  
Shoot Length ◽  
Naphthaleneacetic Acid ◽  
Total Root Length ◽  
Spray Application ◽  
Negative Effects

In five experiments, singlenode cuttings of `Red Cascade' miniature rose (Rosa) were treated with a basal quick-dip (prior to insertion into the rooting substrate) or sprayed to the drip point with a single foliar application (after insertion) of Dip `N Grow [indole-3-butyric acid (IBA) + 1-naphthaleneacetic acid (NAA)], the potassium salt of indole-3-butyric acid (K-IBA), or the potassium salt of 1-naphthaleneacetic acid (K-NAA); a single foliar spray application of Dip `N Grow with and without Kinetic surfactant; or multiple foliar spray applications of Dip `N Grow. Spray treatments were compared with their respective basal quick-dip controls {4920.4 μm [1000 mg·L-1 (ppm)] IBA + 2685.2 μm (500 mg·L-1) NAA, 4144.2 μm (1000 mg·L-1) K-IBA, or 4458.3 μm (1000 mg·L-1) K-NAA}. Cuttings sprayed with 0 to 246.0 μm (50 mg·L-1) IBA + 134.3 μm (25 mg·L-1) NAA, 0 to 207.2 μm (50 mg·L-1) K-IBA, or 0 to 222.9 μm (50 mg·L-1) K-NAA resulted in rooting percentages, total root length, percent rooted cuttings with shoots, and shoot length similar to or less than control cuttings. Exceptions were cuttings sprayed with 0 to 2.23 μm(0.5 mg·L-1) K-NAA, which exhibited shoot length greater than the control cuttings. Addition of 1.0 mL·L-1 (1000 ppm) Kinetic organosilicone surfactant to spray treatments resulted in greater total root length and shoot length. Repeated sprays (daily up to seven consecutive days) had no or negative effects on root and shoot development.

scholarly journals 578 PB 059 IN VITRO ROOTING OF PYRUS GERMPLASM

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 514e-514
Author(s):  
Barbara M. Reed
Keyword(s):  
Butyric Acid ◽  
Growth Regulators ◽  
In Vitro Rooting ◽  
The Other ◽  
Naphthaleneacetic Acid ◽  
Auxin Treatment ◽  
Wide Range ◽  
Hybrid Selection ◽  
Higher Temperature

Cultures of 49 Pyrus species and cultivars and one Pyronia (Pyrus × Cydonia hybrid) selection were screened in vitro to determine a rooting method suitable for a wide range of germplasm. Auxin treatment was required for rooting in most cases. Eighteen of the 50 accessions rooted with a 15 sec. 10 mM indole-3-butyric acid (IBA) dip followed by growth on medium with no growth regulators (NCR). Medium with 10 μM IBA for one week followed by NCR medium produced 12 rooted accessions, but NCR medium alone produced little or no rooting. A 15 sec. dip in 10 mM naphthaleneacetic acid (NAA) followed by NCR medium was tested on 29 accessions which rooted poorly on the other three treatments. Twice as many (28%) rooted on NAA as on either IBA treatment (14% each). Additional treatments combining IBA with darkness or higher temperature were also tested and were successful for some cultivars. P. calleryana, P. koehnei, P. pashia, P. hondoensis, P. ussuriensis, P. betulifolia, P. regelii, P. pyrifolia hybrid cv. Shinseiki and the Pyronia selection failed to root. Twenty two of the 32 P. communis cultivars rooted on at least one treatment.

scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

scholarly journals In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

2018 ◽  
Vol 10 (4) ◽  
pp. 402
Author(s):  
Francisca S. Sá ◽  
Jorge M. P. Porto ◽  
Alone L. Brito ◽  
José R. F. Santana ◽  
Rafaeli A. V. Souza ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Activated Charcoal ◽  
Indole Acetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Indole Butyric Acid ◽  
In Vitro Plantlets ◽  
In Vitro Micropropagation

This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. Nodal segments were obtained from seedlings germinated in vitro with 60 days of life. These were inoculated in MS/2 supplemented with 87.64 µM of sucrose and solidified with 0.7% of agar, supplemented with different concentrations of cytokinin 6-benzylaminopurine (0.0; 2.0; 4.0 and 8.0 µM) associated with different concentrations of auxin, indole acetic acid (0.0; 0.5 and 1.0 µM) and naphthaleneacetic acid (0.0; 0.5 and 1.0 µM). The sprouting were individualized and transferred to MS/2 cultures with different concentrations of indole butyric acid (0.0; 1.0; 2.0 and 3.0 µM), and presence and absence of activated charcoal (1.0 g L-1). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzylaminopurine and indole butyric acid are efficient in micropropagation of B. gardneriana, however, further studies should be performed to optimize this protocol.

scholarly journals Chemical and Biological Stability of Indole-3-Butyric Acid (IBA) After Long-term Storage at Selected Temperatures and Light Regimes

1988 ◽  
Vol 6 (2) ◽  
pp. 39-41 ◽  
Author(s):  
James A. Robbins ◽  
Mark J. Campidonica ◽  
David W. Burger
Keyword(s):  
Butyric Acid ◽  
Storage Temperature ◽  
Naphthaleneacetic Acid ◽  
Light Regimes ◽  
Long Term Storage ◽  
Color Development ◽  
Clear Glass ◽  
Indole 3 Acetic Acid ◽  
Term Storage

Concentrated [4.9 mM (1,000 ppm) and 24.6 mM (5,000 ppm)] IBA solutions in 50% isopropyl alcohol were stored in amber and clear glass bottles at 3 temperatures [22–25°, 6°, O°C (72–77°, 43°, 32°F)]. No significant change in biological activity of the solutions or breakdown of IBA was observed for solutions stored for 4 and 6 months. Solution color changed during storage. Color development was dependant on storage temperature, but not on exposure to light. Chemical names used: IAA = indole-3-acetic acid; IBA = indole-3-butyric acid; NAA = 1-naphthaleneacetic acid

scholarly journals Stimulatory effect of auxins and cytokinins on carotenes, with differential effects on xanthophylls in the green alga Chlorella pyrenoidosa Chick.

2014 ◽  
Vol 66 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Romuald Czerpak ◽  
Andrzej Bajguz
Keyword(s):  
Green Alga ◽  
Butyric Acid ◽  
Chlorella Pyrenoidosa ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Differential Effects ◽  
Green Alga Chlorella ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Β Carotene

Research concerning the influence of auxins and cytokinins on the content of carotenoids in <em>Chlorella pyrenoidosa</em> (<em>Chlorophyceae</em>) has been conducted. The strongest stimulating effect on carotenoids content in <em>Ch. pyrenoidosa</em> biomass was exerted by cytokinins (N-6-benzylaminopurine and N-6-furfurylaminopurine) and allantoin, weaker by auxins and their chemical analogues, and the weakest by tryptamine and 2,4-dichlorophenoxyacetic acid compared to the control. Under the influence of cytokinins the content of α- and β-carotene have been stimulated several times stronger than by auxins, and especially 2,4- dichlorophenoxyacetic acid and tryptamine. However, oxygen-rich xanthophylls content was most strongly reduced by cytokinins (60-70% in relation to the control) in the 20 day lasting of <em>Ch. pyrenoidosa</em> cultivation, similarly to auxins: 1-naphthaleneacetic acid, indole-3-butyric acid, 2,4- dichlorophenoxyacetic acid.

scholarly journals Rooting Responses of Female and Male Cuttings of Leucadendron elimense E. Phillips subsp. elimense on the Type and Concentration of Auxin

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jenny Liedtke ◽  
Muhali Jimoh ◽  
Charles Laubscher
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Root Length ◽  
Restoration Ecology ◽  
Natural Habitat ◽  
Naphthaleneacetic Acid ◽  
Root Number ◽  
Bottom Heat ◽  
Indole 3 Acetic Acid

Abstract Female and male plants of difficult-to-root species Leucadendron elimense subsp. elimense were investigated for rooting potential with three rooting hormones: indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) at three different concentrations (2000 ppm, 4000 ppm, and 6000 ppm). The experiment was made under essential misting, bottom heat, and a naturally ventilated greenhouse. After 18 weeks the experiment terminated and callusing and rooting percentage, root number, and root length were determined. Female cuttings rooted in the highest percentage (80%), root number (>30), and root length (close to 100 mm) after treatment with IAA at 4000 ppm. Under the above treatment male cuttings rooted in 70%, with a mean root number of 24 and root length of 90 mm. The efficient rooting results could aid in saving the species in its natural habitat and supporting restoration ecology, as well as introducing this species into the flower market.

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