scholarly journals Rooting Responses of Female and Male Cuttings of Leucadendron elimense E. Phillips subsp. elimense on the Type and Concentration of Auxin

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jenny Liedtke ◽  
Muhali Jimoh ◽  
Charles Laubscher
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Root Length ◽  
Restoration Ecology ◽  
Natural Habitat ◽  
Naphthaleneacetic Acid ◽  
Root Number ◽  
Bottom Heat ◽  
Indole 3 Acetic Acid

Abstract Female and male plants of difficult-to-root species Leucadendron elimense subsp. elimense were investigated for rooting potential with three rooting hormones: indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) at three different concentrations (2000 ppm, 4000 ppm, and 6000 ppm). The experiment was made under essential misting, bottom heat, and a naturally ventilated greenhouse. After 18 weeks the experiment terminated and callusing and rooting percentage, root number, and root length were determined. Female cuttings rooted in the highest percentage (80%), root number (>30), and root length (close to 100 mm) after treatment with IAA at 4000 ppm. Under the above treatment male cuttings rooted in 70%, with a mean root number of 24 and root length of 90 mm. The efficient rooting results could aid in saving the species in its natural habitat and supporting restoration ecology, as well as introducing this species into the flower market.

Effect of auxin and phenobarbital on the ultrastructure and digitoxin content in Digitalis purpurea tissue culture

1996 ◽  
Vol 74 (3) ◽  
pp. 378-382 ◽  
Author(s):  
Mercedes Bonfill ◽  
Javier Palazón ◽  
Rosa M. Cusidó ◽  
M. Teresa Piñol ◽  
Carmen Morales
Keyword(s):  
Acetic Acid ◽  
Volume Fraction ◽  
Volume Ratio ◽  
Naphthaleneacetic Acid ◽  
Acetic Acid Medium ◽  
Digitalis Purpurea ◽  
Murashige Skoog ◽  
Mitochondrial Volume ◽  
Callus Tissues ◽  
Indole 3 Acetic Acid

Callus derived from Digitalis purpurea hypocotils were grown during a 6-week period on solid Murashige–Skoog medium supplemented with 1 mg/L 6-benzylaminopurine, 0.01 mg/L gibberellic acid and 0.1 mg/L indole-3-acetic acid or α-naphthaleneacetic acid, with or without phenobarbital (40 mg/L). The presence of phenobarbital in the culture medium caused a reduction of the vacuole/cytoplasm ratio. At the same time, the chloroplastic volume fraction decreased in callus tissue cells grown in media supplemented with phenobarbital, while the mitochondrial volume ratio increased. Digitoxin content was enhanced in callus tissues, especially in those grown on indole-3-acetic acid medium supplemented with phenobarbital. The relationship between ultrastructure of D. purpurea callus and digitoxin content is discussed. Keywords: Digitalis purpurea tissue cultures, digitoxin, phenobarbital, mitochondria, chloroplast.

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

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