scholarly journals Stimulatory effect of auxins and cytokinins on carotenes, with differential effects on xanthophylls in the green alga Chlorella pyrenoidosa Chick.

2014 ◽  
Vol 66 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Romuald Czerpak ◽  
Andrzej Bajguz

Research concerning the influence of auxins and cytokinins on the content of carotenoids in <em>Chlorella pyrenoidosa</em> (<em>Chlorophyceae</em>) has been conducted. The strongest stimulating effect on carotenoids content in <em>Ch. pyrenoidosa</em> biomass was exerted by cytokinins (N-6-benzylaminopurine and N-6-furfurylaminopurine) and allantoin, weaker by auxins and their chemical analogues, and the weakest by tryptamine and 2,4-dichlorophenoxyacetic acid compared to the control. Under the influence of cytokinins the content of α- and β-carotene have been stimulated several times stronger than by auxins, and especially 2,4- dichlorophenoxyacetic acid and tryptamine. However, oxygen-rich xanthophylls content was most strongly reduced by cytokinins (60-70% in relation to the control) in the 20 day lasting of <em>Ch. pyrenoidosa</em> cultivation, similarly to auxins: 1-naphthaleneacetic acid, indole-3-butyric acid, 2,4- dichlorophenoxyacetic acid.

1988 ◽  
Vol 18 (7) ◽  
pp. 937-941 ◽  
Author(s):  
M. Rafique Uddin ◽  
Martin M. Meyer Jr. ◽  
J. J. Jokela

Plantlets were obtained by organogenesis from cultured anthers of Populusdeltoides (Bartr.). Anthers formed callus in the dark on modified Murashige and Skoog medium supplemented with 9.0 μM 2,4-dichlorophenoxyacetic acid and 4.7 μM kinetin. Anther calli were differentiated into shoots by sequential transfer in the light onto Murashige and Skoog medium containing 4.4 μM benzylamino purine and 1.1 μM naphthaleneacetic acid for 4 weeks, followed by several transfers to woody plant medium with 2.2 μM benzylamino purine and 1.1 μM naphthaleneacetic acid. The shoots that formed were rooted by excising and transferring to woody plant medium supplemented with 1.0 μM indole-3-butyric acid. A few of these plants were found to be haploid. Two plants developed male terminal inflorescences, but died shortly thereafter.


2016 ◽  
Vol 14 (1) ◽  
pp. 75-86
Author(s):  
Lê Kim Cương ◽  
Nguyễn Hồng Hoàng ◽  
Dương Tấn Nhựt

Ngoc Linh ginseng also known as Vietnamese ginseng (Panax Vietnamensis Ha et Grushv.) is a perennial medicinal plant. This plant is extremely rare and belongs to the Araliaceae family. Scientists are focusing on studies of Ngoc Linh ginseng nowadays. In this research, the effects of 2,4-dichlorophenoxyacetic acid (2,4-D), a-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), 6-benzylaminopurine (BA), Kinetin (KIN), mineral salt formulations and cultural conditions, pH, sucrose concentration, medium volume on cell suspension culture of Panax vietnamensis Ha et Grushv. were investigated. In addition, growth curves and the effect of several plant grow regulators including a-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) on the regeneration of Ngoc Linh ginseng‘s cell suspension were also presented in this study. After 28 days in culture, the results showed that the best growth of a cell suspension of Ngoc Linh ginseng were obtained on ½MS liquid medium supplemented with 1.5 mg/l NAA, 50 g/l sucrose and the most suitable pH was 6.3. The acceptable medium volume for cell suspension growth was 30 ml. The growth curve of Ngoc Linh ginseng’s cell suspension showed that it should be subcultured at the beginning of the stationary phase approximately the 14th-16th day of culture. Ngoc Linh ginseng’s cell suspension exhibited the strongest growth at this time. When Ngoc Linh ginseng’s cell suspension was transferred to fresh medium, somatic embryos were formed in MS medium supplemented with 3.0 mg/l NAA after 30 days culture. The results shown that the potential regeneration of cell suspension of Ngoc Linh ginseng is very high.


2001 ◽  
Vol 66 (4) ◽  
pp. 536-541 ◽  
Author(s):  
J. Ma ◽  
W. Liang ◽  
L. Xu ◽  
S. Wang ◽  
Y. Wei ◽  
...  

1984 ◽  
Vol 62 (7) ◽  
pp. 1393-1397 ◽  
Author(s):  
M. D. Zhou ◽  
T. T. Lee

The callus-promoting activity of most commonly known as well as some rarely tested auxins was compared with that of 2,4-dichlorophenoxyacetic acid (2,4-D) for in vitro culture of the excised embryo of spring and winter wheat (Triticum aestivum L.), cv. Chinese Spring and cv. Fredrick. Different auxins in a concentration range from 1 to 50 μM showed widely different activities. Also the two wheat cultivars responded differently to the auxins. When rapid callus formation with limited root growth was used as the basis for comparison, 2-(2-methyl-4-chlorophenoxy)propionic acid (2-MCPP), α-naphthaleneacetic acid, 3,6-dichloro-2-methoxybenzoic acid (dicamba), 4-amino-3,5,6,trichloropicolinic acid (picloram), γ-(2,4-dichlorophenoxy)butyric acid, 2,4,5-trichlorophenoxyacetic acid, and 2,4,5-trichlorophenoxypropionic acid, in the order of effectiveness, were superior to 2,4,-D for callus induction from the embryo of 'Chinese Spring,' although the concentration required was higher than that of 2,4-D. For the winter wheat 'Fredrick,' however, only picloram, dicamba, and 2-MCPP performed as well as 2,4-D. All auxins tested promoted shoot growth; 2-methyl-4-chlorophenoxypropionic acid was most effective for 'Chinese Spring,' whereas picloram was most effective for 'Fredrick.'


2017 ◽  
Vol 9 (2) ◽  
pp. 27
Author(s):  
Wang Yue ◽  
Sun Fulai ◽  
Gao Qingrong ◽  
Zhang Yanxia ◽  
Wang Nan ◽  
...  

Branched spike wheat is a hexaploid germplasm with branched rachis on its main rachises, and the crucial period for branched rachises occurrence and development is just after the two ridges stage of shoot apex. Natural [indole-3-acetic acid (IAA), indole-3butyric acid (IBA)] and synthetic [(1-naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D)] auxins were applied at this period to investigate the spike traits, seedling growth and photosynthesis related characters and expression of a putative homologue of the LEAFY in branched spike wheat. The four types of experienced auxins induced similar effects on these foresaid characters, although the impact extents were different among the auxins treatments. More branched rachis, spikelets, fertile florets and longer branched rachis were obtained in plants with IAA and IBA at 0.1 mM or NAA and 2,4-D at 1.0mM than those plants with no auxin treated. Auxin treatments also increased fresh and dry mass, photosynthetic pigment and parameters. TFL, a LEAFY-like gene was cloned in branched spike wheat and TFL mRNA expression was quantified using real-time reverse transcriptase-PCR. Application of the auxins accelerated the rise in TFL expression during the periods of branched rachises occurrence and extension. The data supports the hypothesis that auxins play a central role in the regulation branched spike development and TFL might correlate with the development of branched rachises in branched spike wheat.


2007 ◽  
Vol 19 (9) ◽  
pp. 1028-1031 ◽  
Author(s):  
Guo-hua CHANG ◽  
Hao CHEN ◽  
Hai-liu HCEN ◽  
Wei LI ◽  
Gang PAN

1979 ◽  
Vol 15 (1) ◽  
pp. 110-112 ◽  
Author(s):  
Janet Lynn Pelley ◽  
T. T. Bannister

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