scholarly journals Moscato Cerletti, a rediscovered aromatic cultivar with oenological potential in warm and dry areas

OENO One ◽  
2021 ◽  
Vol 55 (3) ◽  
pp. 123-140
Author(s):  
Antonio Sparacio ◽  
Francesco Mercati ◽  
Filippo Sciara ◽  
Antonino Pisciotta ◽  
Felice Capraro ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Historical Documents ◽  
Genetic Analyses ◽  
Changing Climate ◽  
Dry Areas ◽  
Storage Ability

Baron Antonio Mendola was devoted to the study of grapevine, applying ampelography and dabbling in crosses between cultivars in order to select new ones, of which Moscato Cerletti, obtained in 1869, was the most interesting. Grillo, one of the most important white cultivars in Sicily, was ascertained to be an offspring of Catarratto Comune and Zibibbo, the same parents which Mendola claimed he used to obtain Moscato Cerletti. Thus the hypothesis of synonymy between Moscato Cerletti and Grillo or the same parentage for both sets of parents needs to be verified.In the present study, historical documents were consulted and genetic analyses and ampelographic, agronomic and qualitative characterisation carried out to determine the distinctiveness of each cultivars. These were also compared with Catarratto Comune and Zibibbo in order to establish the Moscato Cerletti pedigree. Due to their different SSR profiles, Grillo and Moscato Cerletti were confirmed as two distinct cultivars; they also differed in ripening times and sugar storage ability, as well as in the aromatic grape produced by Moscato Cerletti only. The trio genotype genetic analysis confirmed that Zibibbo is a parent of Moscato Cerletti (justifying the aromatic grape), whilst the SSR profiles did not show Catarratto Comune to be a second parent.Moscato Cerletti was found to have oenological potential in the production of sparkling muscat wines due to its ability to adapt to a changing climate in warm and dry environments and in different winegrowing regions.

scholarly journals Development and characterization of EST-SSR markers for Taxus mairei (Taxaceae) and their transferability across species

2016 ◽  
Vol 65 (1) ◽  
pp. 67-70 ◽  
Author(s):  
X. Wu ◽  
Y. Wen ◽  
S. Ueno ◽  
Y. Tsumura
Keyword(s):  
Molecular Markers ◽  
Genetic Analysis ◽  
Ssr Markers ◽  
Taxus Cuspidata ◽  
Genetic Analyses ◽  
Polymorphic Loci ◽  
Expected Heterozygosity ◽  
Taxus Mairei ◽  
Ssr Loci

AbstractTaxusis an important genus which is well-known for Taxol. Its genetic analyses were lagged behind those of other conifers due to lack of suitable molecular markers. In this paper, we explored polymorphic loci forTaxus maireiand tested their transferability across species based on 150 EST-SSR loci already developed forTaxus cuspidatapreviously. The results showed that 103 loci were polymorphic, the number of alleles per locus ranged from 2 to 11 over 16 individuals. The observed heterozygosity (Ho) and expected heterozygosity (He) varied from 0 to 1 and 0.0625 to 0.891, respectively. ThePICvalues ranged from 0.11 to 0.754 with an average of 0.453. The average cross-species transferability was 96.07% among 5 species. Most of these loci can be used as universal markers inTaxusgenus. The PCA results showed these markers have strong power to identify different species. These markers will be useful for further studies on genetic analysis and conversation ofTaxus mairei.

scholarly journals Cytogenetic definition and morphogenetic analysis of Delta, a gene affecting neurogenesis in Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 118 (2) ◽  
pp. 235-245
Author(s):  
A K Alton ◽  
K Fechtel ◽  
A L Terry ◽  
S B Meikle ◽  
M A Muskavitch
Keyword(s):  
Nervous System ◽  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Inverse Correlation ◽  
Small Interval ◽  
Genetic Analyses ◽  
The Third ◽  
Complementation Groups ◽  
Definition Of ◽  
Morphogenetic Analysis

Abstract We have conducted a genetic analysis of a small interval of the third chromosome known to include Delta (Dl), a locus that affects the segregation of the ectoderm into neural and epidermal lineages during embryogenesis and the morphogenesis of some ectodermally derived structures, in Drosophila melanogaster. This analysis has led to the definition of seven independent complementation groups, one of which is Delta, within the interval extending from 91F6-13 to 92A2. Among the extant mutations in these seven loci, only mutations in Dl lead to the so-called neurogenic phenotype: hypertrophy of the nervous system and reduction of the epidermis. Combined cytogenetic and genetic analyses allow us to define absolute proximal (91F5-92A1) and distal (92A2) cytogenetic limits for the Dl locus. We have isolated hypomorphic and amorphic alleles of Dl and find that, for any given allele, there is an inverse correlation between neural hypertrophy and epidermal reduction in embryos and a direct correlation between the severity of embryonic phenotypes in mutant homozygotes and hemizygotes and the imaginal phenotype in heterozygous adults.

scholarly journals Genetic analysis of axonemal mutants in Paramecium tetraurelia defective in their response to calcium

1984 ◽  
Vol 43 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Robert D. Hinrichsen ◽  
Ching Kung
Keyword(s):  
Genetic Analysis ◽  
Elevated Temperatures ◽  
Complementation Group ◽  
Paramecium Tetraurelia ◽  
Genetic Analyses ◽  
Complementation Groups

SUMMARYSix axonemal mutants of Paramecium tetraurelia have been isolated that are unable to respond properly to calcium. The mutants, designated atalantas, cannot swim backward when stimulated by ions or heat. Genetic analyses reveal that all six mutants are recessive and fall into four complementation groups. Three of the mutants in one complementation group are phenotypically non-leaky, one is leaky and two are extremely leaky, only displaying their phenotypes at elevated temperatures. The complete mutants, ataA, are also abnormal in their forward swimming. This abnormality co-segregates with the inability to swim backward. ataA1 is not allelic to several membrane mutants of P. tetraurelia.

scholarly journals Application of DNA Fingerprints for Identification and Genetic Analyses of Mango (Mangifera indica) Genotypes

1995 ◽  
Vol 120 (2) ◽  
pp. 259-264 ◽  
Author(s):  
A. Adato ◽  
D. Sharon ◽  
U. Lavi ◽  
J. Hillel ◽  
S. Gazit
Keyword(s):  
Genetic Analysis ◽  
Genomic Dna ◽  
Genetic Relatedness ◽  
Mangifera Indica ◽  
Genetic Distances ◽  
Dna Fingerprint ◽  
Dna Fingerprints ◽  
Genetic Analyses ◽  
Young Leaves ◽  
Relatedness Analysis

DNA fingerprint information was used for identification of mango (Mangifera indica L.) cultivars for genetic relatedness analysis of20 mango cultivars and for genetic analysis of a family structure. Genomic DNA was extracted from young leaves, digested with Hind III or Dra I, and hybridized with 10 different DNA probes. Jeffreys' minisatellite probe 33.6 was the most useful, resulting in well-resolved bands representing highly polymorphic loci. Specific patterns were obtained for each cultivar. The probability of obtaining a similar pattern for two different cultivars was 9.4 × 10-6. Based on DNA fingerprint information, genetic distances between 20 mango cultivars were evaluated and an evolutionary tree was established. Analysis of DNA fingerprint band patterns of 12 progeny resulting from a cross between `Tommy Atkins' and `Keitt' mango revealed neither linked nor allelic bands. Application of the reported results for identification, genetic analyses, and mango breeding is discussed.

Tramadol-Related Deaths: Genetic Analysis in Relation to Metabolic Ratios

2021 ◽  
Author(s):  
Sanaa M Aly ◽  
Océane Tartar ◽  
Naoual Sabaouni ◽  
Benjamin Hennart ◽  
Jean-michel Gaulier ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Genetic Variants ◽  
Metabolic Phenotype ◽  
Genetic Analyses ◽  
Blood Concentrations ◽  
Cyp2d6 Phenotype ◽  
Postmortem Blood ◽  
Ultrarapid Metabolizers ◽  
Death Cases

Abstract Tramadol (TR) metabolism is mainly dependent on the enzymatic activity of CYP2D6, which is controlled by genetic polymorphisms. Individuals are classified as poor (PMs), intermediate (IMs), extensive (EMs) or ultrarapid metabolizers (UMs) according to their genotype or phenotype. The determination of the metabolic phenotype for CYP2D6 can be of utmost importance in forensic and clinical contexts that involve TR intake. The present study aimed to describe CYP2D6 genetic variants in cases of TR-related deaths and to assess which metabolic ratio(s) (MRs) would allow to determine CYP2D6 phenotype without having to perform genetic analyses. Forty-eight postmortem blood samples were selected from TR-related death cases previously analyzed in a forensic context in North of France between 2013 and 2019. Initial available data included blood concentrations of TR and its two main metabolites (M1 & M2) determined using a LC-MS/MS method. TR metabolism was expressed as various MRs comprising TR/M1, TR/M2 and M2/M1. After DNA extraction, sequencing was used for genetic variant detections that affect CYP2D6 activity/expression. In the present study, the allelic variants with the higher frequency were CYP2D6*1 (68%), followed by *4 (21%). The most frequent phenotype is EMs (59.6%), followed by IMs (23.4%), PMs (12.8%) and UMs (6.4%). There was no significant correlation between each calculated MR and the genotypically predicted phenotypes, except for M2/M1 which appears related to the PM phenotype. The observed distribution of CYP2D6 genetic variants in this TR-related death population was similar to that found in the general Caucasian population. The present study displayed that the blood M2/M1 ratio could be the best-correlated TR MR to the PM phenotype, and could thus be used in forensic contexts where genetic analyses are not possible or poorly informative. For the other phenotypes, especially the UM phenotype, genetic analysis appears to be the only reliable method to predict the CYP2D6 phenotype.

scholarly journals Genetics of the Deflagellation Pathway in Chlamydomonas

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 927-936
Author(s):  
Rip J Finst ◽  
Peter J Kim ◽  
Lynne M Quarmby
Keyword(s):  
Genetic Analysis ◽  
Transition Zone ◽  
Ultraviolet Irradiation ◽  
Insertional Mutagenesis ◽  
Chemical Stimulus ◽  
Loss Of Function ◽  
Genetic Analyses ◽  
Microtubule Severing ◽  
Nonionic Detergent ◽  
Complementation Groups

Abstract Signal-induced deflagellation in Chlamydomonas involves Ca2+-activated breakage of the nine outer-doublet axonemal microtubules at a specific site in the flagellar transition zone. In this study, we isolated 13 new deflagellation mutants that can be divided into two phenotypic classes, the Adf class and the Fa class. Cells with the Adf deflagellation phenotype are defective in acid-stimulated Ca2+ influx, but can be induced to deflagellate by treatment with nonionic detergent and Ca2+. Genetic analyses show that the five new Adf mutations, as well as the previously identified adf1 mutation, are alleles of the ADF1 gene. Mutants in the second phenotypic class, the Fa mutants, fail to deflagellate in response to any known chemical stimulus and are defective in Ca2+-activated microtubule severing. Genetic analysis of these eight new Fa strains demonstrated that they define two complementation groups, and one of these contains the previously identified fa1 mutation. Diploid analysis showed that five alleles map to the FA1 gene, whereas four alleles define a novel gene that we have named FA2. The isolation of multiple mutant alleles of each gene, generated by either ultraviolet irradiation or insertional mutagenesis, indicates that ADF1, FA1, and FA2 may be the only genes that can be identified in a loss-of-function screen. These alleles should provide a better understanding of the regulation of microtubule severing by Ca2+.

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