scholarly journals Inheritance of the Anasazi Pattern of Partly Colored Seedcoats in Common Bean

2000 ◽  
Vol 125 (3) ◽  
pp. 340-343 ◽  
Author(s):  
Mark J. Bassett ◽  
Kirk Hartel ◽  
Phil McClean
Keyword(s):  
Common Bean ◽  
Genetic Model ◽  
Recessive Gene ◽  
Recurrent Parent ◽  
Allelism Test ◽  
Single Recessive Gene ◽  
Genetic Stock ◽  
Test Cross ◽  
The Cross ◽  
Colored Pattern

Inheritance of Anasazi pattern of partly colored seedcoats in common bean (Phaseolus vulgaris L.) was studied in a genetic stock t ana B V Anasazi BC3 5-593, whose Anasazi pattern is derived from Plant Introduction (PI) 451802. Line 5-593 is a determinate, Florida dry bean breeding line (with small black seeds) used as the recurrent parent in the development of many genetic stocks. The F2 from the cross t ana B V Anasazi BC3 5-593 × t z virgarcus BC3 5-593 segregated for two nonparental phenotypic classes and was consistent with the hypothesis that a single recessive gene, with tentative symbol ana, produces the Anasazi pattern with t Z ana and a new partly colored pattern Anabip with t z ana. Thus, the Anasazi factor is not an allele at the Z locus. Analysis of 57 random F3 progenies from the cross t ana B V Anasazi BC3 5-593 × t z virgarcus BC3 5-593 supported a genetic model where: 1) with t Z the Anasazi phenotype is controlled by a single recessive gene ana, i.e., genotype t Z ana, 2) the Anabip phenotype has the genotype t z ana, and 3) t Z/z ana produces a restricted Anasazi pattern. The allelism test cross t z ana Anabip BC3 5-593 × t z lers white BC3 5-593 produced complementation in the F2, demonstrating nonallelism of Ana (actually Bip) with the L locus. The allelism test cross t z ana Anabip BC3 5-593 × t z bip bipunctata BC3 5-593 failed to show complementation in F1 and F2, demonstrating allelism of Ana with the Bip locus. Using bulk segregant analysis, molecular markers linked in coupling to the Ana (OM9200, 5.4 cM) and Bip (OJ17700, 6.0 cM) genes were discovered. Allelism was also suggested by the result that the same linkage distance and recombination pattern were observed when the Ana marker was used to score the bipunctata population. We propose the gene symbol bipana for the recessive allele at the Bip locus that produces Anasazi pattern with genotype t Z bipana and the Anabip pattern with genotype t z bipana. Although bipana and bip are both recessive to Bip, their interactions with the Z locus are extraordinarily different. The pattern restrictive power of bipana expresses partly colored pattern with t Z, whereas bip requires t z to express partly colored pattern.

scholarly journals Inheritance of the Partly Colored Seedcoat Pattern, Bipunctata, in Common Bean

1996 ◽  
Vol 121 (6) ◽  
pp. 1032-1034 ◽  
Author(s):  
Mark J. Bassett
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Genetic Background ◽  
Recessive Gene ◽  
Recurrent Parent ◽  
Single Recessive Gene ◽  
Complete Dominance ◽  
The Cross

The development of genetic tester stocks in common bean (Phaseolus vulgaris L.) for the partly colored seedcoat patterns `bipunctata BC3 5-593' (t z bip) and `virgarcus BC3 5-593' (t z) was described. The inheritance of the bipunctata pattern was studied in the F2 from the crosses `bipunctata BC1 5-593' × 5-593 and `bipunctata BC2 5-593' × 5-593. The data supported the hypothesis that a single recessive gene (bip) converts virgarcus (t z Bip) to bipunctata (t z bip). The inheritance of bipunctata was also studied in the F2 from the cross `bipunctata BC3 5-593' × `virgarcus BC3 5-593'. The data supported the hypothesis of complete dominance of Bip over bip in a t z genetic background highly related to the recurrent parent 5-593, where only the parental phenotypes appear in the F2.

scholarly journals Interaction of Two Genes, Fcr and Fcr2, with the t Allele in Common Bean that Restores Color to Flowers

1993 ◽  
Vol 118 (6) ◽  
pp. 881-884 ◽  
Author(s):  
Mark J. Bassett
Keyword(s):  
Common Bean ◽  
Genetic Model ◽  
Flower Color ◽  
Segregation Ratio ◽  
Plant Introduction ◽  
White Flower ◽  
Recurrent Parent ◽  
Genetic Stock ◽  
Dry Bean ◽  
Flower Plant

Plant Introduction (PI) accession 507984 of common bean (Phaseolus vulgaris L.) has partly colored seed coats and either pure white flowers or light laelia flowers. Crosses were made with white-flowered plants of PI 507984: white-flowered plant #1 × the genetic stock t ers ers2 BC2 5-593 and white-flowered plant #2 × recurrent parent dry bean breeding line 5-593. Inheritance was studied in the F1, F2, and F3 of the former cross and the F1 and F2 of the latter cross. PI 507984 (white flower, plant #1) × t ers ers2 BC2 5-593 gave F1 plants with colored flowers and partly colored seeds. The F2 gave a 9:7 segregation ratio (colored flowers to white flowers), and the genetic model proposed is that flower color is restored in the presence of t/t by two complementary genes, Fcr and Fcr-2. That model was confirmed by F3 progeny tests of 21 F2 parents with colored flowers. The cross PI 507984 (white flower, plant #2) × 5-593 gave an F2 segregation ratio of 9:3:4 (bishops-violet: light laelia: white flowers), indicating that the white-flowered PI 507984 has vlae masked by t. Analysis of all the data suggests that PI 507984 is heterogeneous at Fcr and Fcr-2, having all three possible homozygous genotypes, viz., either light laelia flowers from vlae t Fcr Fcr-2 or white flowers from vlae t Fcr fcr-2 or vlae t fcr Fcr-2. The flower color restoration gene in 5-593 is arbitrarily assigned the symbol Fcr. Great variability occurs in partly colored seeds of PI 507984 due to the environment in which the seed was produced.

scholarly journals Classical and Molecular Genetic Studies of the Strong Greenish Yellow Seedcoat Color in `Wagenaar' and `Enola' Common Bean

2002 ◽  
Vol 127 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Mark J. Bassett ◽  
Rian Lee ◽  
Carla Otto ◽  
Phillip E. McClean
Keyword(s):  
Common Bean ◽  
Color Change ◽  
Molecular Genetic ◽  
Recessive Gene ◽  
Recurrent Parent ◽  
Dry Bean ◽  
Greenish Yellow ◽  
The Cross ◽  
Black Seeds ◽  
Relationship Of

Inheritance of the strong greenish-yellow (SGY) seedcoat color in `Wagenaar' common bean (Phaseolus vulgaris L.) was investigated. Line 5-593 is a determinate, Florida dry bean breeding line (with small black seeds) used as the recurrent parent in the development of many genetic stocks, e.g., g b v BC3 5-593. Through crosses with genetic tester stocks, the seedcoat genotype of `Wagenaar' was confirmed to be C J g b vlae Rk. Three randomly amplified polymorphic DNA markers (OAP7850, OAP31400, and OU14950) that cosegregated with the G seedcoat color locus were developed from the F2 population derived from the cross g b v BC2 5-593 × G b v BC3 5-593. From the cross `Wagenaar' × g b v BC3 5-593, 80 F2 plants were classified into 54 non-SGY and 16 SGY seedcoat color plants. When the OAP7850 marker was applied to that population, linkage was not observed with the non-SGY and SGY phenotypes. Conversely, a molecular marker (OAP12400, that was developed from the F2 from the cross `Wagenaar' × g b v BC3 5-593) linked to the locus controlling the SGY phenotype segregated independently of the G locus. Therefore, SGY phenotype is not controlled by the G locus. An F3 progeny test of 76 F2 plants from the cross `Wagenaar' × g b v BC3 5-593 confirmed the hypothesis that a single recessive gene (for which we propose the symbol gy) controls the seedcoat color change from pale greenish yellow (PGY) to SGY. Through crosses with genetic tester stocks, the seedcoat genotype of `Enola' was determined to be C J g b vlae Rk. The test cross `Enola' × `Wagenaar' demonstrated that `Enola' also carries the gy gene. The relationship of `Enola' to the `Mayocoba' market class of common bean and to `Azufrado Peruano 87' is discussed.

scholarly journals The Margo (mar) Seedcoat Color Gene Is a Synonym for the Joker (j) Locus in Common Bean

1996 ◽  
Vol 121 (6) ◽  
pp. 1028-1031 ◽  
Author(s):  
Mark J. Bassett
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Recurrent Parent ◽  
Allelism Test ◽  
Genetic Loci ◽  
Common Gene ◽  
Parent Stock ◽  
The Common ◽  
Color Gene

The inheritance of hilum ring color in common bean (Phaseolus vulgaris L.) was investigated using various genetic tester stocks developed by backcrossing recessive alleles into a recurrent parent stock 5-593 with seedcoat genotype P [C r] D J G B V Rk, viz., mar BC2 5-593, mar BC3 5-593, mar v BC2 5-593, mar d BC2 5-593, and mar d BC3 5-593. The current hypothesis is that the margo character is controlled by mar and hilum ring color is controlled by d but expresses only with mar. The V locus controls flower and seedcoat color. The allelism test crosses `Citroen' (P C d j g b vlae) × mar BC3 5-593 and `Citroen' × mar d BC3 5-593 demonstrated that mar is allelic with j and that the putative d in mar d BC3 5-593 is allelic with the d in `Citroen'. Thus, the former genetic tester stocks mar BC3 5-593 and mar d BC3 5-593 are reclassified as j BC3 5-593 and d j BC3 5-593, respectively, because mar is a synonym for j. Similarly, the former genetic tester stock mar v BC2 5-593 is reclassified as j v BC2 5-593. The interaction of j with d expresses as loss of color in the hilum ring. The development of the white-seeded genetic tester stock P cu d j BC3 5-593 was described in detail, where the all-recessive tester `Prakken 75' was used as the source of the recessive alleles. The previously reported work showing that the partly colored seedcoat gene t interacts with mar to control seedcoat pattern is now interpreted to mean that the joker (J) locus interacts with t to produce partly colored seedcoat patterns. The genetic loci D and V were found to segregate independently. The common gene for dull seedcoats (asper, asp) is discussed and contrasted with j.

scholarly journals A Pleiotropic Mutant for Male Sterility and Spindly Branching at the sb Locus in Common Bean

1991 ◽  
Vol 116 (2) ◽  
pp. 346-348
Author(s):  
Mark J. Bassett
Keyword(s):  
Common Bean ◽  
Male Sterility ◽  
Recessive Gene ◽  
Single Recessive Gene ◽  
Backcross Populations ◽  
Allelism Tests ◽  
Pleiotropic Gene ◽  
Induced Mutant ◽  
Pleiotropic Mutant ◽  
Extensive Breeding

The inheritance of an induced mutant for spindly branch and male sterility (SBMS) was investigated in common bean (Phaseolus vulgaris L.) in F2 and backcross populations. The results support the hypothesis that the mutant is controlled by a single recessive gene. Extensive breeding work with SBMS, involving several thousand F2 progeny, produced no recombinant of the types expected if two closely linked genes controlled the character. Therefore, a single pleiotropic gene apparently controls SBMS. Allelism tests demonstrated that SBMS is allelic with sb but not with sb-2 and sb-3. The gene symbol sbms is proposed for SBMS because it is a new allele at sb, with the order of dominance being Sb > sb > sbms. Various ways to exploit the new mutant for marked male sterility are discussed.

scholarly journals An Induced Mutant for Blue Flowers in Common Bean That Is Not Allelic to V or Sal and Is Linked to Fin

1992 ◽  
Vol 117 (2) ◽  
pp. 317-320 ◽  
Author(s):  
Mark J. Bassett
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Methyl Violet ◽  
Recessive Gene ◽  
Flower Color ◽  
Single Recessive Gene ◽  
Large Variability ◽  
Allelism Tests ◽  
Plant Habit ◽  
Induced Mutant

A mutation for blue (methyl-violet) flower color was induced by gamma irradiation of dry seed in common bean (Phaseolus vulgaris L.). Inheritance and allelism tests demonstrated that the mutation is controlled by a single recessive gene that is not allelic with Sal or V. The gene symbol blu is proposed for this mutant. Linkage was detected between blu and Fin, which controls the change from indeterminate to determinate plant habit. Three linkage estimates ranged from 19 to 35 cM, but the large variability (homogeneity x2 = 45.32) precludes making a conclusive combined estimate of linkage. The tentative combined estimate of linkage is 27 cM.

scholarly journals Characterization and Inheritance of Four Induced Leaf Mutants in Common Bean

1992 ◽  
Vol 117 (3) ◽  
pp. 512-514
Author(s):  
Mark J. Bassett
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Genetic Markers ◽  
Gamma Rays ◽  
Recessive Gene ◽  
Single Recessive Gene ◽  
Leaf Mutants ◽  
Lanceolate Leaf ◽  
Gene Symbols

Dry seeds of common bean (Phaseolus vulgaris L.) were treated with 20 krad (1 rad = 0.01 Gy) of gamma rays to induce plant mutations to be used as genetic markers in mapping studies. Four leaf mutants are described and illustrated. Inheritance studies demonstrated that each is controlled by a single recessive gene. The proposed gene symbols are: cml for chlorotic moderately lanceolate leaf, lbd for leaf-bleaching dwarf, glb for glossy bronzing leaf, and 01 for overlapping leaflets. Linkage tests involving cml and nine previously reported marker mutants failed to detect any linkages.

scholarly journals The Gametic Non-Lethal Gene Gal on Chromosome 5 Is Indispensable for the Transmission of the Co-Induced Semidwarfing Gene d60 in Rice

Biology ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 94
Author(s):  
Motonori Tomita ◽  
Takatoshi Tanisaka
Keyword(s):  
Genetic Model ◽  
Recessive Gene ◽  
Male Gamete ◽  
Lethal Gene ◽  
Single Recessive Gene ◽  
Pollen Mitosis ◽  
Chromosome 5 ◽  
Second Pollen Mitosis ◽  
Generation Sequencing ◽  
Simple Sequence

The gametic lethal gene gal in combination with the semidwarfing gene d60 causes complementary lethality in rice. Here, we attempted to ascertain the existence of gal and clarify male gamete abortion caused by d60 and gal. Through the F2 to F4 generations derived from the cross between D60gal-homozygous and d60Gal-homozygous, progenies of the partial sterile plants (D60d60Galgal) were segregated in a ratio of 1 semidwarf (1 d60d60GalGal):2 tall and quarter sterile (2 D60d60Galgal):6 tall (2 D60d60GalGal:1 D60D60GalGal:2 D60D60Galgal:1 D60D60galgal), which is skewed from the Mendelian ratio of 1 semidwarf:3 tall. However, the F4 generation was derived from fertile and tall heterozygous F2 plants (D60d60GalGal), which were segregated in the Mendelian ratio of 1[semidwarf (d60d60GalGal)]:2[1 semidwarf:3 tall (D60d60GalGal)]:1[tall (D60D60GalGal)]. The backcrossing of D60Gal-homozygous tall F4 plants with Hokuriku 100 resulted in fertile BCF1 and BCF2 segregated in a ratio of 1 semidwarf:3 tall, proving that d60 is inherited as a single recessive gene in the D60d60GalGal genetic background (i.e., in the absence of gal). Further, gal was localized on chromosome 5, which is evident from the deviated segregation of d1 as 1:8 and linkage with simple sequence repeat (SSR) markers. Next-generation sequencing identified the candidate SNP responsible for Gal. In F1 and sterile F2, at the binucleate stage, partial pollen discontinued development. Degraded pollen lost vegetative nuclei, but second pollen mitosis raising two generative nuclei was observed. Thus, our study describes a novel genetic model for a reproductive barrier. This is the first report on such a complementary lethal gene, whose mutation allows the transmission of a co-induced valuable semidwarfing gene d60.

scholarly journals Inheritance of Twisted Pods in Common Bean (Phaseolus vulgaris L.)

1995 ◽  
Vol 120 (6) ◽  
pp. 900-901
Author(s):  
J.R. Baggett ◽  
D. Kean
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Recessive Gene ◽  
Green Bean ◽  
Single Recessive Gene ◽  
Phaseolus Vulgaris L ◽  
Normal Parent

Inheritance of a twisted pod characteristic, in which bean pods develop with a twist that sometimes exceeds 360°, was studied in crosses between round-podded green bean cultivars. In crosses between `Oregon 91G' (normal) or `Oregon 54' (normal) and OSU 5256-1 (twisted), the F1 was normal. Segregation in F2 populations, tested over a 4-year period and including 4,995 plants, clearly fit a 3 normal: 1 twisted ratio. All plants of backcrosses of the F, to the normal parent were normal and backcrosses of the F1 to the twisted parent segregated 1 normal: 1 twisted. The ratios observed indicated that twisted pods are conditioned by a single recessive gene for which the symbol tw is proposed.

scholarly journals A New Allele (Vwf) at the V Locus for Flower and Seedcoat Color in Common Bean

1997 ◽  
Vol 122 (4) ◽  
pp. 519-521 ◽  
Author(s):  
Mark J. Bassett
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Test Cross ◽  
The Cross ◽  
Black Seeds

The inheritance of flower and seedcoat color was studied using Lamprecht line M0137 (PI 527845) of common bean (Phaseolus vulgaris L.) as the source of a new allele, Vwf, at the V locus. The cross M0137 cres Vwf × C v BC2 5-593 (a genetic tester stock) was studied in progeny of the F1, F2, F3, and F4 generations. The observed segregation for flower and seed colors was consistent with the hypothesis that M0137 carried a new allele, Vwf, that produced (in the presence of P C J G B) white flowers and black seeds rather than the white flowers and mineral-brown seeds produced (in the presence of P C J G B) by v. The V/Vwf genotype produced cobalt-violet flowers, the same as V/v. A test cross of F3 Vwf × t BC1 5-593 bipunctata demonstrated that Vwf is not allelic with t, a gene that can produce white or colored flowers and self-colored or partly colored seeds, depending on background genotype.

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