scholarly journals AFLP Markers Linked to Eastern Filbert Blight Resistance from OSU 408.040 Hazelnut

2005 ◽  
Vol 130 (3) ◽  
pp. 412-417 ◽  
Author(s):  
Honglin Chen ◽  
Shawn A. Mehlenbacher ◽  
David C. Smith
Keyword(s):  
Fragment Length Polymorphism ◽  
Dominant Gene ◽  
Aflp Markers ◽  
Blight Resistance ◽  
Resistance Locus ◽  
Willamette Valley ◽  
Negative Segregation ◽  
Anisogramma Anomala ◽  
Selection For ◽  
Eastern Filbert Blight

Eastern filbert blight (EFB), caused by Anisogramma anomala (Peck) E. Müller, is a devastating disease to european hazelnut (Corylus avellana L.) orchards in the Willamette Valley of Oregon. Selection OSU 408.040 showed no symptoms or signs of the fungus following greenhouse inoculations, and enzyme-linked immunosorbant assays (ELISAs) were negative. Segregation ratios in three progenies indicate that a single dominant gene controls the resistance. A total of 64 amplified fragment length polymorphism (AFLP) primer combinations were screened using three resistant and three susceptible individuals as well as the parents of the cross OSU 245.098 × OSU 408.040. Primer combinations that showed no more than one recombinant in these six seedlings were investigated in 30 additional seedlings. Markers that showed <15% recombination with resistance were amplified in the remaining seedlings of the population. Five AFLP markers linked in coupling to resistance were identified. B2-125 was located on one side of the resistance locus at a distance of 4.1 centimorgans (cM), while A4-265 (9.2 cM), C2-175 (5.9 cM) and D8-350 (2.5 cM) were on the other side, and A8-150 cosegregated with resistance. Three of these markers (B2-125, C2-175, and D8-350) were also linked in coupling in a similar order in seedlings from a second progeny. These markers may be useful in marker-assisted selection for eastern filbert blight resistance from hazelnut selection OSU 408.040.

scholarly journals Identification and Mapping of DNA Markers Linked to Eastern Filbert Blight Resistance from OSU 408.040 Hazelnut

HortScience ◽  
2012 ◽  
Vol 47 (5) ◽  
pp. 570-573 ◽  
Author(s):  
Vidyasagar R. Sathuvalli ◽  
Shawn A. Mehlenbacher ◽  
David C. Smith
Keyword(s):  
Ssr Markers ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Resistance ◽  
Resistance Locus ◽  
State University ◽  
Willamette Valley ◽  
Sources Of Resistance ◽  
Disease Marker ◽  
Anisogramma Anomala ◽  
Eastern Filbert Blight

Eastern filbert blight (EFB) of European hazelnut (Corylus avellana L.), caused by the pyrenomycete Anisogramma anomala (Peck) E. Müller, is a major disease problem and production constraint in Oregon’s Willamette Valley. Host genetic resistance is viewed as the most economical means of controlling this disease. Marker-assisted selection has been extensively used for ‘Gasaway’ resistance in the hazelnut breeding program at Oregon State University (OSU). Concern over potential breakdown of this single resistance gene prompted a search for new sources of resistance. Selection OSU 408.040 showed no signs or symptoms of the fungus after a series of disease inoculations, and resistance was transmitted to half of its offspring, indicating control by a dominant allele at a single locus. In this study, we identified six random amplified polymorphic DNA (RAPD) and 11 simple sequence repeat (SSR) markers linked to EFB resistance from OSU 408.040. The new markers supplement the previously identified amplified fragment length polymorphism (AFLP) markers. A linkage map constructed in the progeny OSU 245.098 × OSU 408.040 spanned a distance of 19.5 cM with the resistance locus cosegregating with AFLP marker A8-150 and located between SSR markers LG675 and LG682. Using SSR markers as anchor loci, OSU 408.040 resistance was assigned to linkage group 6 (LG6). Comparison with the previously mapped ‘Gasaway’ resistance locus showed that resistance from OSU 408.040 maps to the same location.

scholarly journals Identification of Amplified Fragment Length Polymorphism Markers Linked to Gummy Stem Blight (Didymella bryoniae) Resistance in Melon (Cucumis melo L.) PI 420145

HortScience ◽  
2009 ◽  
Vol 44 (1) ◽  
pp. 32-34 ◽  
Author(s):  
Joseph N. Wolukau ◽  
Xiaohui Zhou ◽  
JinFeng Chen
Keyword(s):  
Molecular Markers ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Dominant Gene ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Didymella Bryoniae ◽  
Stem Blight ◽  
Gummy Stem Blight

Gummy stem blight (GSB) caused by the ascomycete fungus Didymella bryoniae (Auersw.) Rehm is an important disease of melon. Molecular markers linked to resistance would be useful for melon breeding programs. The amplified fragment length polymorphism (AFLP) technique and bulk segregant analysis were used to identify molecular markers linked to the resistance of melon to Didymella bryoniae. Segregation analysis of F2 progeny from a cross of PI 420145, a resistant line, and PI 136170, a susceptible line, showed that resistance to GSB was controlled by a dominant gene. One AFLP marker, E-TG/M-CTC200, was identified that is tightly linked to GSB resistance gene at a distance of 2.0 cM. To our best knowledge, this is the first report of AFLP markers linked to GSB resistance in melon. The identification of AFLP markers provides a step toward the use of marker-assisted selection and the characterization of the gene encoding resistance to GSB in melon.

scholarly journals Genetic Analysis and Identification of Amplified Fragment Length Polymorphism Markers Linked to the alm1 Avirulence Gene of Leptosphaeria maculans

1998 ◽  
Vol 88 (10) ◽  
pp. 1068-1072 ◽  
Author(s):  
Patchara Pongam ◽  
Thomas C. Osborn ◽  
Paul H. Williams
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Leptosphaeria Maculans ◽  
Amplified Fragment Length Polymorphism ◽  
Gene Interaction ◽  
Aflp Markers ◽  
Avirulence Gene ◽  
Resistance Locus ◽  
Gene For Gene

A gene-for-gene interaction was previously suggested by mapping of a single major locus (LEM 1) controlling cotyledon resistance to Leptosphaeria maculans isolate PHW1245 in Brassica napus cv. Major. In this study, we obtained further evidence of a gene-for-gene interaction by studying the inheritance of the corresponding avirulence gene in L. maculans isolate PHW1245. The analysis of segregating F1 progenies and 14 test crosses suggested that a single major gene is involved in the interaction. This putative avirulence gene was designated alm1 after the resistance locus identified in B. napus. Amplified fragment length polymorphism (AFLP) markers were used to generate a rudimentary genetic linkage map of the L. maculans genome and to locate markers linked to the putative avirulence locus. Two flanking AFLP markers, AC/TCC-1 and AC/CAG-5, were linked to alm1 at 3.1 and 8.1 cM, respectively. Identification of markers linked to the avirulence gene indicated that the differential interaction is controlled by a single gene difference between parental isolates and provides further support for the gene-for-gene relationship in the Leptosphaeria-Brassica system.

scholarly journals 521 PB 483 RESISTANCE TO EASTERN FILBERT BLIGHT IN CORYLUS SPECIES AND INTERSPECIFIC HYBRIDS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 506b-506
Author(s):  
Glare J. Coyne ◽  
Shawn A. Mehlenbacher
Keyword(s):  
New York ◽  
Interspecific Hybrid ◽  
Interspecific Hybrids ◽  
Dominant Gene ◽  
Single Dominant Gene ◽  
Sources Of Resistance ◽  
Anisogramma Anomala ◽  
Eastern Filbert Blight ◽  
Serious Disease ◽  
Kernel Traits

Eastern filbert blight (EFB) (Anisogramma anomala) is a serious disease of the European hazelnut (Coryls avellana). A single dominant gene for immunity to EFB from C. avellana `Gasaway' is being combined with good nut and kernel traits using a modified backcross approach. Additional sources of resistance would be highly desirable. Clones and seedlings of six other species (C. columa, C. comuta, C. heterophylla, C. sieboldiana, C. amencana, and C. jaquemontii] and a few interspecific hybrid selections were screened in the greenhouse to identify new sources of resistance. C. jacquemontii seedlings and C. columa clones were highly susceptible. C. comuta, C. hetemphylla, and C. sieboldiana clones were resistant, as were 86% of the C. americana seedlings tested. Five C. americana × C. avellana hybrids from New York were resistant under field conditions. One of four C. comuta × C. avellana and two of three C. hetemphylla × C. avellana hybrids were resistant.

scholarly journals Identification of Co-1 Anthracnose Resistance and Linked Molecular Markers in Common Bean Line A193

Plant Disease ◽  
2001 ◽  
Vol 85 (3) ◽  
pp. 252-255 ◽  
Author(s):  
Azucena Mendoza ◽  
Fernando Hernández ◽  
Sanjuana Hernández ◽  
Daniel Ruíz ◽  
Octavio Martínez de la Vega ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Marker Assisted Selection ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Colletotrichum Lindemuthianum ◽  
Single Dominant Gene ◽  
Selection For ◽  
Race 2

Phaseolus vulgaris line A193 has been shown to be widely resistant to Colletotrichum lindemuthianum, including race 1472, one of the most virulent races of C. lindemuthianum characterized. Resistance to C. lindemuthianum race 1472 in P. vulgaris line A193 was investigated in segregating F2 and F2.3 populations from a cross between A193 and Flor de Mayo Bajio (a commercial cultivar highly susceptible to C. lindemuthianum). Resistance to 1472 in A193 was determined to be conditioned by a single dominant gene. Inoculation of crosses between A193 and cultivars Michigan Dark Red Kidney and Perry Marrow with race 1472 suggest that resistance in A193 is conditioned by the Co-1 gene. Inoculation of the cross A193 × Perry Marrow with C. lindemuthianum race 2, demonstrated that resistance to race 2 in Perry Marrow is also conditioned by a single dominant gene and is distinct to resistance in A193 or Michigan Dark Red Kidney. Three AFLP markers (ECAG/MACC-1, EACA/MAGA-2, EAGG/MAAC-8) linked in repulsion to the Co-1 locus were identified by screening the A193 × Flor de Mayo F2 population with 314 random amplified polymorphic DNA, amplified fragment length polymorphism, and restriction fragment length polymorphism markers. The two most closely linked markers should be useful in marker-assisted selection for Co-1.

scholarly journals Sources of Resistance to Eastern Filbert Blight in Hazelnut

1998 ◽  
Vol 123 (2) ◽  
pp. 253-257 ◽  
Author(s):  
Clarice J. Coyne ◽  
Shawn A. Mehlenbacher ◽  
David C. Smith
Keyword(s):  
Dominant Gene ◽  
Genetic Resistance ◽  
Resistance Breeding ◽  
The United States ◽  
Corylus Avellana ◽  
Sources Of Resistance ◽  
Corylus Cornuta ◽  
Anisogramma Anomala ◽  
Eastern Filbert Blight ◽  
Significant Disease

Eastern filbert blight is an economically significant disease in European hazelnut (Corylus avellana L.) production in the United States. Since genetic resistance is the only viable disease control strategy to this fungal disease caused by Anisogramma anomala (Peck) E. Müller, greenhouse and field screening of germplasm was undertaken to study the inheritance from known resistant sources and to identify new sources for inclusion in the breeding program. We confirmed that `Gasaway' resistance to this disease is conferred by a single dominant gene. No major gen was identified in the field-resistant cultivar Gem. Representatives of six Corylus species were screened to identify new resistant germplasm. Corylus cornuta Marshall var. cornuta, C. cornuta var. californica (A.DC.) Sharp, C. heterophylla Fischer, and C. sieboldiana Blume were highly resistant, as were most C. americana Marshall genotypes and one C. colurna L. clone tested, but C. jacquenontii Decaisne was highly susceptible. In several cases, hybrids of these species with susceptible C. avellana were also resistant. These new sources of resisstance are being incorporated in the resistance breeding effort.

Mapping a gene conferring resistance to Pseudocercosporella herpotrichoides on chromosome 4V of Dasypyrum villosum in a wheat background

Genome ◽  
1998 ◽  
Vol 41 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Ahmet Yildirim ◽  
Stephen S Jones ◽  
Timothy D Murray
Keyword(s):  
Distal Part ◽  
Dominant Gene ◽  
Rflp Markers ◽  
Addition Line ◽  
Dasypyrum Villosum ◽  
Pseudocercosporella Herpotrichoides ◽  
Eyespot Resistance ◽  
Double Recombination ◽  
Simultaneous Selection ◽  
Selection For

The objectives of this study were to map and tag the previously undescribed eyespot resistance gene PchDv on chromosome 4V of Dasypyrum villosum in a wheat background. The 82 F2 plants used for mapping were produced from a cross between a susceptible\i wheat 'Yangmai-5' (4V(4D)) substitution line and a resistant wheat 'Chinese Spring' disomic addition line of chromosome 4V of D. villosum. Segregation for resistance and susceptibility among F2 plants was 3:1, indicating that resistance was controlled by a single dominant gene. PchDv mapped to the distal part of chromosome 4V and was bracketed by two RFLP markers, Xcdo949 and Xbcd588, in a 33-cM interval. This distance could not be reduced, owing to a lack of polymorphic loci in this region. Theoretically, double recombination in this region occurs in 3.3% of the individuals; therefore, 96.7% of the selected genotypes would have PchDv, with simultaneous selection for both flanking markers. Double recombination between the flanking markers was observed in 2 out of 82 (2.4%) F2 individuals.

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