scholarly journals Changes of Antioxidant Defense System and Fatty Acid Composition in Bermudagrass under Chilling Stress

2017 ◽  
Vol 142 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Zhengrong Hu ◽  
Erick Amombo ◽  
Margaret Mukami Gitau ◽  
Aoyue Bi ◽  
Huihui Zhu ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Low Temperature ◽  
Acid Composition ◽  
Antioxidant Defense ◽  
Chilling Stress ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Total Glutathione ◽  
Binding Factor

Bermudagrass (Cynodon dactylon) is a typical and widely used warm-season turfgrass. Low temperature is one of the key environmental stress limiting its utility. However, little information is available about the differences of cold response between bermudagrass genotypes. Here, we analyzed antioxidant defense system and fatty acid composition in cold-resistant genotype WBD128 and cold-sensitive genotype WBDg17 exposed to chilling stress. Low temperature (4 °C) significantly decreased the relative water content, whereas increased the H2O2 and O2− contents, more profoundly for WBDg17. Under chilling condition, WBD128 had higher anti O2− activity than WBDg17. Besides, the contents of total glutathione, reduced glutathione (GSH) and its oxidized form (GSSG) were markedly increased by low temperature in both genotypes, whereas WBD128 had significantly higher values of GSH, total glutathione, and GSH/GSSG ratio than WBDg17. Moreover, chilling stress increased saturated fatty acids (SFAs) percentage (palmitic acid and stearic acid) in WBDg17. After chilling treatment, the proportion of linoleic acid decreased in both genotypes, particularly in WBDg17. As for unsaturated fatty acids (UFAs), the percentage of linolenic acid was increased in WBD128. In addition, chilling treatment decreased the values of double bond index (DBI), UFA/SFA ratio as well as degree of unsaturation in WBDg17. Finally, chilling stress altered the expression patterns of the genes, which encode one kind of late embryogenesis abundant proteins (LEA), superoxide dismutase (Cu/Zn SOD) C-repeat-binding factor/DRE-binding factor (CBF1), and peroxidase (POD-2). Collectively, our results revealed that natural variation of chilling tolerance in bermudagrass genotypes may be largely associated with the alterations of antioxidant defense system and fatty acid composition.

Effect of fabA and desA Mediated Low Temperature Adaption on Shewanella putrefaciens via Regulation of Unsaturated Fatty Acid Metabolism

2019 ◽  
Vol 11 (10) ◽  
pp. 1430-1437
Author(s):  
Li Chen ◽  
Shengping Yang ◽  
Yunfang Qian ◽  
Jing Xie
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Low Temperature ◽  
Acid Composition ◽  
Fatty Acid Metabolism ◽  
Unsaturated Fatty Acid ◽  
Acid Metabolism ◽  
Shewanella Putrefaciens ◽  
Spoilage Bacteria ◽  
Temperature Environment

Shewanella putrefaciensis a kind of spoilage bacteria in low temperature chilled aquatic products, which seriously threats human health and aquaculture. The fatty acid composition of S. putrefaciens cell membranes has been shown to be involved in adaption of bacteria to various environments. However, the specific fatty acid metabolism of S. putrefaciens to the low temperature environment remains unknown. In this study, the growth of S. putrefaciens, the response of fatty acid composition to low temperature production, and the differential expression and synthesis of enzymes related to unsaturated fatty acid synthesis were investigated by lack of fabA and desA in S. putrefaciens. Results showed that loss of fabA and desA suppressed the growth of S. putrefaciens and reduced unsaturated fatty acid contents at low temperature. In addition, the upregulation of fabA, but not desA resulted in accumulation of unsaturated fatty acid. Up-regulations of fabA and desA both resulted in promotion of GPR41 and Retn gene and protein expressions. These results demonstrated that the deletions of fabA and desA resulted in reduction of unsaturated fatty acid and key downstream genes of fatty acid metabolism, which suggested that unsaturated fatty acid was involved in the adaptations of fabA and desA-mediated S. putrefaciens to the low temperature environment. These results provided a tentative mechanism of the synthesis of unsaturated fatty acids in S. putrefaciens under low temperature conditions.

The effect of low temperature on fatty acid composition and tocopherols of the red microalga, Porphyridium cruentum

2007 ◽  
Vol 19 (3) ◽  
pp. 223-227 ◽  
Author(s):  
Yaşar Durmaz ◽  
Margarida Monteiro ◽  
Narcisa Bandarra ◽  
Şevket Gökpinar ◽  
Oya Işik
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Low Temperature ◽  
Acid Composition ◽  
Porphyridium Cruentum ◽  
Red Microalga

scholarly journals Changes in GSH-antioxidant system induced by daunorubicin in human normal and diabetic fibroblasts.

2003 ◽  
Vol 50 (3) ◽  
pp. 825-835 ◽  
Author(s):  
Agnieszka Zatorska ◽  
Janusz Maszewski ◽  
Zofia Jóźwiak
Keyword(s):  
Antioxidant Defense ◽  
Reductase Activity ◽  
Human Fibroblasts ◽  
Antioxidant Defense System ◽  
Free Medium ◽  
Defense System ◽  
Glutathione S Transferase ◽  
Total Glutathione ◽  
Glutathione Reductase Activity ◽  
Drug Free

We investigated the effect of daunorubicin on glutathione content and activity of GSH-related enzymes in cultured normal and diabetic human fibroblasts. Cells were incubated with 4 microM daunorubicin (DNR) for 2 h followed by culture in drug-free medium for up to 72 h. Treatment of diabetic cells with the drug caused a time-dependent depletion of intracellular GSH and a decrease of the GSH to total glutathione ratio. GSH depletion was accompanied by apoptotic changes in morphology of the nucleus. Analysis of GSH-related enzymes showed a significant increase of the activities of Se-dependent and Se-independent peroxidases and glutathione S-transferase. In contrast, glutathione reductase activity was reduced by 50%. Significant differences between normal and diabetic cells exposed to DNR were observed in the level of GST and Se-dependent glutathione peroxidase activities. These findings indicated that daunorubicin efficiently affects the GSH antioxidant defense system both in normal and diabetic fibroblasts leading to disturbances in glutathione content as well as in the activity of GSH-related enzymes.

Sign in / Sign up

Export Citation Format

Share Document