scholarly journals Biocontrol Potential of Entomopathogenic Nematodes Heterorhabditis and Steinernema Against Second Instar Grub of White grub, Holotrichia serrata Fab. Infesting Sugarcane

2015 ◽  
Vol 4 (12) ◽  
pp. 1267-1269
Zootaxa ◽  
2020 ◽  
Vol 4878 (1) ◽  
pp. 77-102 ◽  
Author(s):  
AASHA RANA ◽  
AASHAQ HUSSAIN BHAT ◽  
ASHOK KUMAR CHAUBEY ◽  
EBRAHIM SHOKOOHI ◽  
RICARDO A. R. MACHADO

Agricultural soils and open fields from Western Uttar Pradesh (India) were surveyed to determine the presence of entomopathogenic nematodes. From the entomopathogenic nematodes isolated, Heterorhabditis isolates were selected and further characterized using morphological, morphometrical and molecular approaches. The results showed that three isolated nematodes were Heterorhabditis bacteriophora and were associated with Photorhabdus laumondii subsp. clarkei bacteria, while the rests were identified as Heterorhabditis indica. The biocontrol potential of H. bacteriophora against three agricultural pests was evaluated. Nematode infectivity experiments showed that the nematode isolates DH7 and DH8 were highly pathogenic against cotton bollworm (Helicoverpa armigera) and tobacco cutworm (Spodoptera litura), and less pathogenic against white grub (Holotrichia serrata) larvae. This study sets the basis for establishing new biocontrol agents to be used in pest management programs in India.


2012 ◽  
Vol 22 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Lemma Ebssa ◽  
Eugene M. Fuzy ◽  
Matthew W. Bickerton ◽  
Albrecht M. Koppenhöfer

Nematology ◽  
2020 ◽  
Vol 22 (10) ◽  
pp. 1111-1120
Author(s):  
Wenxiu Guo ◽  
Guoyu Zhao ◽  
Congli Wang ◽  
Xianhong Zhou ◽  
Yifan Zhai ◽  
...  

Summary Anomala corpulenta is one of the major white grubs that cause serious damages in peanut production. To develop an environmentally friendly method to control A. corpulenta larvae, the efficacy of species of entomopathogenic nematodes (EPN), application rate and larval stage against A. corpulenta were determined. Results showed that Steinernema longicaudum X-7 and S. glaseri B-4-1 were the most virulent species, which caused 77.8 and 84.4% corrected mortalities of the 2nd instar larvae at 25°C 14 days after treatments. Corrected mortalities of A. corpulenta caused by EPN were significantly affected by EPN application rate. A decrease but no significant difference in susceptibility from the 2nd to the 3rd instar was observed for the test EPN species S. longicaudum, S. glaseri and Heterorhabditis bacteriophora. The field experiments indicated that S. longicaudum and S. glaseri applied at a relatively high rate (⩾5.0 × 103 infective juveniles (IJ) plant−1, i.e., 7.5 × 108 IJ ha−1) were able to provide not only control efficacy against A. corpulenta with ⩾92.7% reduction of larvae and ⩽5.06% of damaged pods, and the peanut yield increase was as good as phoxim, but also gave better control persistency compared to phoxim. Our findings indicated that EPN could be an effective strategy for the management of A. corpulenta in peanut fields.


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