scholarly journals Study into biodegradation of cocamidopropyl betaine, an amphoteric surfactant, by Pseudomonas bacteria and activated sludge

2021 ◽  
Vol 11 (3) ◽  
pp. 441-448
Author(s):  
A. S. Burlachenko ◽  
O. V. Salishcheva ◽  
L. S. Dyshlyuk
Keyword(s):  
Activated Sludge ◽  
Pseudomonas Putida ◽  
Pseudomonas Fluorescens ◽  
Half Life ◽  
Pseudomonas Stutzeri ◽  
Minimum Time ◽  
Time Interval ◽  
Pseudomonas Mendocina ◽  
Pseudomonas Oleovorans ◽  
Cocamidopropyl Betaine

Abstract: The paper examines the biodegradation rate of cocamidopropyl betaine by bacteria of the genus Pseudomonas and activated sludge. The following microorganisms were taken as destructor strains: Pseudomonas fluorescens TR (VKPM B-4881), Pseudomonas putida TP-19 (B-6582), Pseudomonas stutzeri T (B-4904), Pseudomonas putida TSh-18 (B-2950), Pseudomonas putida TO (B-3959), Pseudomonas mendocina 2S (B-4710), Pseudomonas oleovorans TF4-1L (B-8621) and activated sludge obtained at activated sludge reactors of a Kuzbass plant. Biooxidation of surfactant samples was carried out in 250 cm3 glass flasks, placed into an incubator shaker, at a constant temperature of 30ºС for pure cultures and 18ºС for activated sludge. The destructor strain should reduce the surfactant concentration to safe values within a minimum time interval. Pseudomonas stutzeri T (B-4904) and Pseudomonas fluorescens TR (B-4881) strains provided the shortest half-life of the surfactant under study – 2.5 and 2.6 days, respectively. For Pseudomonas putida TO (B-3959), Pseudomonas putida TSh-18 (B-2950) and Pseudomonas oleovorans TF4-1L (B-8621) strains, these values amounted to 3.0, 4.5 and 4.9 days, respectively. The maximum half-life of the surfactant under study was demonstrated by Pseudomonas mendocina 2S (B-4710) and Pseudomonas putida TP-19 (B-6582) microorganisms – 5.5 and 6.0 days, respectively. The maximum biodegradation of the surfactant was observed under its exposure to the biocenosis of microorganisms. Over 14 days, the concentration of cocamidopropyl betaine decreased to 0.27% of its initial concentration. The efficiency of Pseudomonas bacteria as destructors of surfactants was demonstrated. Bacteria of this genus exhibit a shorter generation time and a higher rate of biomass growth when compared to other strains and a shorter period of adaptation to surfactants when compared to activated sludge. Capable of reducing surfactant concentrations to safe values in a minimum time interval, Pseudomonas strains can be used as an effective agent in the development of technologies for wastewater purification from amphoteric surfactants.

Characterization of saccharolytic nonfermentative bacteria associated with man

1970 ◽  
Vol 16 (5) ◽  
pp. 351-362 ◽  
Author(s):  
M. J. Pickett ◽  
Margaret M. Pedersen
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Pseudomonas Fluorescens ◽  
Pseudomonas Stutzeri ◽  
Clinical Isolates ◽  
Gram Negative ◽  
Clinical Strains ◽  
Pseudomonas Maltophilia ◽  
Reference Strains

Features of 378 clinical isolates of saccharolytic, nonfermentative Gram-negative rods and 20 reference strains were examined. All but four of the clinical strains were assigned to recognized taxa, namely Acinetobacter, Chromobacterium, Flavobacterium, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas maltophilia, Pseudomonas multivorans, Pseudomonas putida, Pseudomonas stutzeri, and Xanthomonas.

scholarly journals Rizobacterias con potencial antagonista in vitro a Mycosphaerella fijiensis Morelet

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Karen Tatiana Chávez Arteaga ◽  
Jefferson Javier Guato Molina ◽  
Jorge Luis Rodríguez Acosta ◽  
Ángel Virgilio Cedeño Moreira ◽  
Ricardo Fernando Romero Meza ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Serratia Marcescens ◽  
Pseudomonas Fluorescens ◽  
Mycosphaerella Fijiensis ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Pseudomonas Veronii ◽  
Enterobacter Asburiae

El empleo de bio-controladores en la agricultura beneficia los aspectos fisiológicos en plantas, a diferencia de la constante aplicación de pesticidas en el cultivo del banano ha ocasionado la pérdida de la sensibilidad en M. fijiensis, reduciendo la microbiota del suelo. El objetivo se enfocó en caracterizar el potencial antagónico de las PGPR en inhibición de germinación de ascósporas y desarrollo micelial de M. fijiensis. Se realizaron cultivos monospóricos de M. fijiensis e identificado por PCR. Se evaluaron los extractos celulares de Pseudomonas putida PB3-6, Klebsiella variicola BO3-4, Enterobacter asburiae BA4-19, Serratia marcescens PM3-8, Enterobacter asburiae PM3-14, Pseudomonas protegens CHA0, Pseudomonas fluorescens WCS417, Pseudomonas veronii R4 y Bacillus subtilis ATCC 5540 para sus evaluaciones antagonistas: a) Inhibición del tubo germinativo de las ascósporas al 2% y b) Desarrollo micelial al (2 y 10 %). La PCR empleado en la identificación de M. fijiensis se confirma el producto de amplificación de 1018 pb. El factor antagónico de los extractos celulares al 2 % de PM3-14 y CHA0 inhibe sobre el 80 % al desarrollo de los tubos germinativos. La inhibición al desarrollo micelial del extracto celular al 2 %, de CHA0 logró una efectividad del 54 % y las cepas (PM3-8, PM3-14 y BA4-19) con (32, 26 y 26 %). Al 10 % del extracto de la cepa PM3-8 inhibe el desarrollo micelial con niveles de turbidez de 0,47 (OD600nm). El empleo de estos bio-controladores en la agricultura ofrecerá una alternativa para beneficiar en la reducción del uso de agroquímicos

scholarly journals Genome-wide analysis of the FleQ direct regulon in Pseudomonas fluorescens F113 and Pseudomonas putida KT2440

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Esther Blanco-Romero ◽  
Miguel Redondo-Nieto ◽  
Francisco Martínez-Granero ◽  
Daniel Garrido-Sanz ◽  
Maria Isabel Ramos-González ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Pseudomonas Fluorescens ◽  
Pseudomonas Putida Kt2440 ◽  
Genome Wide Analysis ◽  
Genome Wide

Microbiology of activated sludge treating wastewater containing Ni(II) and Cr(VI)

1996 ◽  
Vol 34 (5-6) ◽  
pp. 183-191 ◽  
Author(s):  
Filiz B. Dilek ◽  
Celal F. Gökçay
Keyword(s):  
Activated Sludge ◽  
Bacillus Cereus ◽  
Laboratory Scale ◽  
Pseudomonas Mendocina ◽  
Biochemical Tests ◽  
Arthrobacter Simplex ◽  
Simulated Wastewater ◽  
Acinetobacter Sp

The species of microorganisms dominating a laboratory-scale once-through activated sludge unit treating simulated wastewater with different influent strengths (650 and 1300 mgl−1 COD) and containing 5 to 10 mgl−1 Ni(II) and 1 to 50 mgl−1 Cr(VI) were identified by standard biochemical tests. Within this framework, combinations of metals and influent COD concentrations were tried. Organisms, namely Proteus rettgeri, Acinetobacter sp., Flavobacterium lutescens, Bacillus cereus var. albolactis, Klebsiella sp., Arthrobacter simplex, and Pseudomonas mendocina, were identified at different stages of the experiments.

Effects of PGPR (Pseudomonas sp.) and Ag-nanoparticles on Enzymatic Activity and Physiology of Cucumber

2020 ◽  
Vol 11 (2) ◽  
pp. 124-136 ◽  
Author(s):  
Sehrish Nawaz ◽  
Asghari Bano
Keyword(s):  
Superoxide Dismutase ◽  
Pseudomonas Putida ◽  
Ag Nanoparticles ◽  
Phenylalanine Ammonia Lyase ◽  
Combined Treatment ◽  
Pseudomonas Stutzeri ◽  
Plant Growth Promoting Rhizobacteria ◽  
Vegetative Phase ◽  
Pal Activity ◽  
Plant Growth Promoting

Background: The present investigation aimed to evaluate the role of Plant Growth- Promoting Rhizobacteria (PGPR) and Ag-nanoparticles on two varieties (American variety, Poinsett 76 and Desi variety, Sialkot selection) of cucumber plants. Methods: Cucumber seeds prior to sowing, were inoculated with two strains of PGPR, Pseudomonas putida (KX574857) and Pseudomonas stutzeri (KX574858) at the rate of 106 cells/ml. Agnanoparticles (5ppm) were sprayed on the plant at early vegetative phase 27 d after sowing. Results: The proline, sugar, protein, phenolics, flavonoids, chlorophyll and carotenoids contents of leaves of plants and the activities of Phenylalanine Ammonia-Lyase (PAL), Superoxide Dismutase (SOD) and Catalase (CAT) were determined from leaves of plants at early vegetative phase. After 3 months of seeds sowing, Ag-nanoparticles enhanced the length of root but decreased the length of shoot and fresh weight of root and shoot as compared to control whereas, the leaf protein, proline, phenolics, flavonoids, chlorophyll b, total chlorophyll, sugar and Phenylalanine Ammonia-Lyase (PAL) activity of plants were increased significantly over control. Ag-nanoparticles also suppressed the effect of PGPR for root, shoot length but augmented the protein and phenolics contents of leaves of both the varieties. Conclusion: The combined treatment of Ag-nanoparticles and PGPR enhanced flavonoids content of leaves and the activities of PAL, SOD and CAT in leaves of plants over control. Agnanoparticles effectively increased the Phenylalanine Ammonia-Lyase (PAL), Catalase (CAT) and superoxide dismutase (SOD) activities in leaves of both the varieties. Pseudomonas putida may be used either alone or in combination with Ag-nanoparticles to enhance the antioxidant and defense enzyme activities. Hence, the plant can tolerate the diseases and stresses in a much better way with higher protein and phenolics content.

scholarly journals IMP-1 and a Novel Metallo-β-Lactamase, VIM-6, in Fluorescent Pseudomonads Isolated in Singapore

2004 ◽  
Vol 48 (6) ◽  
pp. 2334-2336 ◽  
Author(s):  
Tse Hsien Koh ◽  
Grace Chee Yeng Wang ◽  
Li-Hwei Sng
Keyword(s):  
Pseudomonas Putida ◽  
Pseudomonas Fluorescens ◽  
Fluorescent Pseudomonads ◽  
Gene Variant ◽  
Original Sequence ◽  
Carbapenem Resistant

ABSTRACT Four carbapenem-resistant Pseudomonas spp. were isolated from patients in Singapore. One Pseudomonas putida isolate contained a bla IMP-1 identical to that first described in Japan. The sequence of a variant bla IMP-1 in Pseudomonas fluorescens contained four silent mutations compared with the original sequence. The remaining P. putida isolates contained bla VIM-6, a novel VIM gene variant.

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