scholarly journals Microcirculation Disorders in Patients with Severe COVID-19

2021 ◽  
Vol 18 (4) ◽  
pp. 7-19
Author(s):  
E. E. Ladozhskaya-Gapeenko ◽  
K. N. Khrapov ◽  
Yu. S. Polushin ◽  
I. V. Shlyk ◽  
N. N. Petrishchev ◽  
...  
Keyword(s):  
Blood Flow ◽  
Endothelial Dysfunction ◽  
Capillary Blood ◽  
Linear Velocity ◽  
Capillary Blood Flow ◽  
Control Group ◽  
Doppler Flowmetry ◽  
Occlusion Test ◽  
Cuff Deflation ◽  
Significant Difference

Impaired microcirculation due  to endothelial dysfunction in COVID-19  is considered  to be  the most important link in the pathogenesis of this disease. However, due  to  the  complexity of its  instrumental  assessment  in critically ill patients,  the data available  in  the  literature on specific manifestations of endothelial dysfunction are very contradictory.The objective:  to determine  the most characteristic capillaroscopic signs of microvascular disorders and  to assess  the  state of microcirculation regulation in patients with severe COVID-19.Subjects  and Methods. When admitted  to  the  intensive  care unit,  60 patients with  COVID-19  and  12  patients with chronic cardiovascular pathology without COVID-19 (Comparison Group) were examined. All patients underwent microscopy of the microcirculatory bed of finger nail bed; the following parameters were assessed: diameters of the venous, arterial and transitional parts of capillaries, height of capillary loops, density of capillaries per  1 mm of the  length of the perivascular zone,  the average  linear velocity of capillary blood  flow (LVCBF), and  thickness of the perivascular zone. The presence of avascular zones,  the number of capillaries in the visualized field with circulating aggregates in the lumen, and the shape of capillaries were taken into account.  In addition, an occlusion test using laser Doppler flowmetry was performed  in 32 patients with COVID-19. The maximum post-occlusive increase in blood flow at the moment of cuff deflation was assessed, as well as changes in the mean value of post-occlusive blood flow relative to the baseline within 3 minutes after cuff deflation.Results.  In 53 (88.3%) patients with COVID-19, abnormalities corresponding to chronic microcirculatory changes in the form of predominance of pathological capillary forms were detected. Microaggregates in the lumen of capillaries and decreased linear velocity of blood flow were revealed in 100% of cases. When comparing groups of patients with different outcomes, statistically significant differences were revealed between the LVCBF parameters (in the survivors -  354.35 ± 44.78 pm/sec, in the deceased - 278.4 ± 26.59 pm/sec), as well as between the values of the perivascular zones thickness  (95.35  ±  15.96 microns versus  159.93 ±  19.90 microns). The results of the post-occlusion  test revealed  a significant difference between the groups in terms of the maximum post-occlusion gain (39.42 ± 3.85 BPU in the group with a favorable outcome, 27.69 ± 3.19 BPU in the group with an unfavorable outcome, 47.23 ±  1.78 BPU in the control group). In both groups, there was no increase in this parameter relative to the initial blood flow. At the same time, in the control group, the average index of post-occlusive blood flow was higher than the initial level.Conclusions. Acute microcirculation disorders with decreased linear velocity of capillary blood flow, circulation of aggregates, increased thickness of the perivascular zone were detected in all patients with severe COVID-19 but especially in those with unfavorable outcomes. Vascular tone regulation disorders were manifested by the absence of reactive hyperemia in response to acute ischemia, as well as a decrease in maximal flow-induced increase. These changes fit into the concept of endothelial dysfunction. Signs of chronic microcirculation disorders in most patients increase the risk of severe COVID-19.

scholarly journals Pancreatic capillary blood flow during caerulein-induced pancreatitis evaluated by a laser-doppler flowmeter in rats

2003 ◽  
Vol 18 (suppl 5) ◽  
pp. 18-22 ◽  
Author(s):  
Roberto Ferreira Meirelles Jr. ◽  
Reginaldo Ceneviva ◽  
José Liberato Ferreira Caboclo ◽  
Michael M. Eisenberg
Keyword(s):  
Blood Flow ◽  
Serum Amylase ◽  
Tissue Injury ◽  
Capillary Blood ◽  
Laser Doppler ◽  
Capillary Blood Flow ◽  
Control Group ◽  
Laser Doppler Flowmeter ◽  
Significant Difference ◽  
Doppler Flowmeter

PURPOSE: The pancreatic capillary blood flow (PCBF) was studied to determine its alterations during caerulein-induced pancreatitis in rats. METHODS: Twenty rats were divided in groups: control and caerulein. A laser-Doppler flowmeter to measure PCBF continuously was used. Blood pressure (BP) and heart rate (HR) were monitored. Serum biochemistry analyses were determined. Histopathological study was performed. RESULTS: The PCBF measured a mean of 109.08 ± 14.54% and 68.24 ± 10.47% in control group and caerulein group, respectively. Caerulein group had a mean decrease of 31.75 ± 16.79%. The serum amylase was 1323.70 ± 239.10U.I-1 and 2184.60 ± 700.46U.I-1 in control and caerulein groups, respectively. There was a significant difference in the PCBF (p<0.05) and serum amylase (p<0.05) when compared to control and caerulein groups. Although micro and microvacuolization were seen in 30% in caerulein group, no significant difference was seen between the groups. CONCLUSION: A decrease in the PCBF may be one of the leading events and it is present before histopathological tissue injury had been established in this model of acute pancreatitis.

THE ROLE OF LASER AND ULTRASONIC DOPPLER FLOWMETRY IN THE CORRECTION OF COSMETIC DEFECTS OF THE ANTERIOR ABDOMINAL WALL AND PREVENTION OF COMPLICATIONS

2021 ◽  
Vol 74 (7) ◽  
pp. 1605-1611
Author(s):  
Orest V. Panchuk ◽  
Yaroslav M. Susak ◽  
Ievgen G. Donets ◽  
Pavlo l. Byck ◽  
Olena F. Panchuk ◽  
...  
Keyword(s):  
Blood Flow ◽  
Abdominal Wall ◽  
Anterior Abdominal Wall ◽  
Main Group ◽  
Control Group ◽  
Entire Study ◽  
Doppler Flowmetry ◽  
Significant Difference ◽  
Simultaneous Operations ◽  
Ultrasonic Doppler Flowmetry

The aim: Of work is to determine changes in blood flow in the vessels of the anterior abdominal wall that occur after plastic surgeries in order to improve the results of operations and to develop new methods for the prevention of complications. Materials and methods: The study was conducted in 132 patients. Patients were divided into 2 groups: main group 64 and control group 68 patients. Main group has patients who underwent abdominoplasty in combination with liposuction; control group has patients who underwent abdominoplasty without liposuction. In both groups we make different simultaneous operations. Laser Doppler Flowmetry and Ultrasonic Doppler Flowmetry were performed to determine the blood flow indices in the flaps. Results: Liposuction volumes averaged 3.57 ± 0.74 liters of lipoaspirate. In the main group there were totaly 4 complications, in the control group complications developed in 9 patients. Comparing daily indicators between the two groups, no statistically significant difference in the dynamics of MI changes was found during the entire study period (p = 0.767). Increase in caliber of vessels, on average, from 1.55 ± 0.8 mm in the preoperative period to 1.68 ± 0.75 mm on the 14th day of the postoperative period was statistically significant (p < 0.05). Conclusions: The combination of abdominoplasty with liposuction and simultaneous operations does not lead to greater development of complications and allows to achieve good aesthetic results.

scholarly journals CORRELATION BETWEEN THE RATE OF DECOMPENSATION IN SPINAL MEMBRANE BLOOD CIRCULATION, THE MAGNITUDE OF SPINAL DEFORMITY AND THE OUTCOMES OF SURGICAL TREATMENT IN PATIENTS WITH IDIOPATHIC SCOLIOSIS

2006 ◽  
pp. 026-032
Author(s):  
Еlena Nikolayevna Schurova ◽  
Aleksandr Timofeyevich Khudyaev ◽  
Pavel Ivanovich Kovalenko
Keyword(s):  
Blood Flow ◽  
Surgical Treatment ◽  
Idiopathic Scoliosis ◽  
Spinal Deformity ◽  
Capillary Blood ◽  
Surgical Correction ◽  
Capillary Blood Flow ◽  
Spine Deformity ◽  
Doppler Flowmetry ◽  
Adjacent Segments

Objective. To study microcirculation in spinal membranes, and its correlation with the magnitude of spinal deformity and the outcomes of surgical treatment in patients with grade III–IV idiopathic scoliosis. Material and Methods. Intraoperative laser Doppler flowmetry was performed to measure microcirculation in spinal membranes at the deformity apex and adjacent segments during surgical correction of scoliosis in 10 patients at the age of 14 to 17 years. Results. It was revealed that the volumetric capillary blood flow in spinal membranes at the deformity apex was decreased by 27–57 % as compared to sufficient level. Decompensation of microcirculation in membranes was more expressed in Sshaped deformity. Blood flow reduction is in direct relation to the angle of spine deformity. Spine deformity correction with intraoperative distraction measuring from 5 to 20 mm could cause neurologic complications if blood flow in spinal membranes at the apex of deformity is less than 20ml/min per 100 g and cranial blood flow ranges from 18 to 40 ml/min per 100 g. These patients should be treated with a more sparing intraoperative distraction of the spine (≤5 mm) followed by conservative enhancement of spinal cord microcirculation. Conclusion. Parameters of volumetric capillary blood flow in spinal membranes at the deformity apex and adjacent segments could be used for prediction of neurological complications in surgical correction of idiopathic scoliosis.

Measurement of Muscle Surface Capillary Blood Flow by Laser Doppler Flowmetry

1992 ◽  
Vol 21 (6) ◽  
pp. 491-493 ◽  
Author(s):  
WENDY M. NORMAN ◽  
MICHAEL H. COURT ◽  
NICHOLAS H. DODMAN ◽  
FRANK S. PIPERS
Keyword(s):  
Blood Flow ◽  
Laser Doppler Flowmetry ◽  
Capillary Blood ◽  
Laser Doppler ◽  
Capillary Blood Flow ◽  
Doppler Flowmetry

scholarly journals Scanning Laser Doppler Flowmetry Shows Reduced Retinal Capillary Blood Flow in CADASIL

Stroke ◽  
2004 ◽  
Vol 35 (11) ◽  
pp. 2449-2452 ◽  
Author(s):  
Mika Harju ◽  
Susanna Tuominen ◽  
Paula Summanen ◽  
Matti Viitanen ◽  
Minna Pöyhönen ◽  
...  
Keyword(s):  
Blood Flow ◽  
Laser Doppler Flowmetry ◽  
Capillary Blood ◽  
Laser Doppler ◽  
Capillary Blood Flow ◽  
Scanning Laser ◽  
Doppler Flowmetry ◽  
Retinal Capillary ◽  
Scanning Laser Doppler Flowmetry

scholarly journals Microcirculatory parameters in compensated and decompensated type 2 diabetes mellitus

2017 ◽  
Vol 89 (10) ◽  
pp. 28-35 ◽  
Author(s):  
O V Suchkova ◽  
Yu I Gurfinkel ◽  
M L Sasonko
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Blood Flow ◽  
Type 2 Diabetes Mellitus ◽  
Capillary Blood ◽  
Capillary Blood Flow ◽  
Control Group ◽  
Functional Changes ◽  
Group 2

Aim. To reveal the features of microcirculatory parameters in compensated and decompensated type 2 diabetes mellitus (T2DM). Subjects and methods. A total of 196 patients with T2DM were examined and divided into 2 groups: 1) 52 patients (40.4% of men) aged 52.8±8.7 years with compensated T2DM (glycated hemoglobin (HbA1с), 6.3±0.5%); 2) 68 patients (38.2% of men) aged 52.8±8.1 years with decompensated T2DM (HbA1с, 9.4±1.7%). Both patient groups had concomitant hypertension (its prevalence, degree, stage of hypertension were comparable). A control group consisted of 76 volunteers (40.8% of men) aged 52.2±8.7 years with normal carbohydrate metabolism and without signs of cardiovascular disease (HbA1с, 5.3±0.49%). Capillary blood flow in the finger nail-fold area was investigated in all the participants. A digital optical capillaroscope with image-processing software was used to obtain quantitative blood microcirculatory parameters. The diameters of arterial and venous capillary segments were measured, by calculating the remodeling rate. The degree of capillary tortuosity, network density, and polymorphism and the size of the perivascular zone (PZ) were estimated. Blood rheological properties and capillary blood flow velocity were also investigated. Results. The decompensated T2DM group compared to the compensated T2DM group was found to have a narrowing of the arterial capillary segment diameter (8.4±2.0 µm; p=0.009) and an increase in remodeling rates (1.47±0.22; p=0.000). The tendency of the PZ size to be larger in patients with decompensated T2DM compared to those with compensated T2DM (p=0.080) and the increase in this indicator compared to the control group (p=0.001) reflect the presence of edema syndrome in Group 2, as laboratory confirmed by a statistically significantly elevated sodium level (p=0.000; p=0.006). The enlarged venous capillary segment demonstrates involvement of the venous component in microcirculatory disorders in T2DM. The reduction in the density of the capillary network and the increase in capillary tortuosity and polymorphism, which were also observed in the patients of both groups versus the control group, are referred to as disorders that are characteristic of T2DM. Conclusion. In decompensated T2DM, capillary bed structural and functional changes are found as a narrowing of the arterial capillary segment, an increase in the rate of remodeling, and enlargement of the PZ. Digital capillaroscopy opens up new possibilities for assessing the magnitude of changes in the microcirculatory system in DM and can simultaneously evaluate the efficiency of treatment, by monitoring the status of the microvasculature.

State of some indices of laser doppler flowmetry in norm and in gingivitis in children

1999 ◽  
Vol 80 (4) ◽  
pp. 287-290
Author(s):  
M. Z. Mirgazizov ◽  
N. K. Khamitova ◽  
E. V. Mamaeva
Keyword(s):  
Blood Flow ◽  
Early Diagnosis ◽  
Laser Doppler Flowmetry ◽  
Capillary Blood ◽  
Laser Doppler ◽  
Capillary Blood Flow ◽  
Doppler Flowmetry ◽  
Microcirculatory Disorders

Capillary blood flow of paradont tissue in norm and in children sufferred from chronic generalized gingivitis and paradontosis in slight form is studied. The method of estimating microcirculatory disorders in paradont tissue using laser doppler fluorometry is developed. The prospects of its wide use in stomatology for early diagnosis of disorders of paradont capillary net-work are stressed.

scholarly journals Reactive oxygen species inactivation improves pancreatic capillary blood flow in caerulein-induced pancreatitis in rats

2003 ◽  
Vol 18 (suppl 5) ◽  
pp. 29-33 ◽  
Author(s):  
Roberto Ferreira Meirelles Jr. ◽  
Reginaldo Ceneviva ◽  
José Liberato Ferreira Caboclo ◽  
Michael M. Eisenberg
Keyword(s):  
Reactive Oxygen Species ◽  
Blood Flow ◽  
Serum Amylase ◽  
Reactive Oxygen ◽  
Serum Biochemistry ◽  
Capillary Blood ◽  
Capillary Blood Flow ◽  
Oxygen Species ◽  
Significant Difference ◽  
Qualitative Changes

PURPOSE: Reactive oxygen species (ROS) inactivation was studied to determine alterations in the pancreatic capillary blood flow (PCBF) during caerulein-induced pancreatitis in rats. METHODS: A laser-Doppler flowmeter to measure PCBF and N-t-Butyl-Phenylnitrone (PBN) compound to inactivate ROS were used. Forty rats were divided in groups: 1) control; 2) caerulein; 3) PBN; 4) caerulein+PBN. Serum biochemistry and histopathological analyses were performed. RESULTS: PCBF measured a mean of 109.08 ± 14.54%, 68.24 ± 10.47%, 102.18 ± 10.23% and 87.73 ± 18.72% in groups 1, 2, 3 and 4, respectively. PCBF in groups 2 and 4 decreased 31.75 ± 16.79% and 12.26 ± 15.24%, respectively. Serum amylase was 1323.70 ± 239.10 U/l, 2184.60 ± 700.46 U/l, 1379.80 ± 265.72 U/l and 1622.10 ± 314.60 U/l in groups 1, 2, 3 and 4, respectively. There was a significant difference in the PCBF and serum amylase when compared groups 2 and 4. Cytoplasmatic vacuolation was present in groups 2 and 4. Otherwise, no qualitative changes were seen. CONCLUSION: ROS inactivation improves PCBF and minimizes the serum amylase increase during caerulein-induced pancreatitis. ROS effect may be one of the leading causative events in this model of acute pancreatitis.

Sign in / Sign up

Export Citation Format

Share Document