3D-Printed Replica and Porcine Explants for Pre-Clinical Optimization of Endoscopic Tumor Treatment by Magnetic Targeting

Background: Animal models have limitations in cancer research, especially regarding anatomy-specific questions. An example is the exact endoscopic placement of magnetic field traps for the targeting of therapeutic nanoparticles. Three-dimensional-printed human replicas may be used to overcome these pitfalls. Methods: We developed a transparent method to fabricate a patient-specific replica, allowing for a broad scope of application. As an example, we then additively manufactured the relevant organs of a patient with locally advanced pancreatic ductal adenocarcinoma. We performed experimental design investigations for a magnetic field trap and explored the best fixation methods on an explanted porcine stomach wall. Results: We describe in detail the eight-step development of a 3D replica from CT data. To guide further users in their decisions, a morphologic box was created. Endoscopies were performed on the replica and the resulting magnetic field was investigated. The best fixation method to hold the magnetic field traps stably in place was the fixation of loops at the stomach wall with endoscopic single-use clips. Conclusions: Using only open access software, the developed method may be used for a variety of cancer-related research questions. A detailed description of the workflow allows one to produce a 3D replica for research or training purposes at low costs.

Ritter (A. Ritter, Beitrag zur Kenntniss des Neurinoms des Magens. "Schw. Med. W." No. 50, 1931)

(A. Ritter, Beitrag zur Kenntniss des Neurinoms des Magens. "Schw. Med. W." No. 50. 1931) describes a rare case of gastric neuroma. During the operation, a tumor the size of a hazelnut 3: 3 tbsp. Was removed from the stomach wall (small curvature, approximately in the middle part of the body). Metastases were not found anywhere.

Treatment of stomach and duodenal ulcers. Grlässner (Wiener kl Woch .. 1921, no. 47)

Grlssner (Wiener kl Woch .. 1921, No. 47) warmly recommends for this purpose a 0.2-0.4% solution of caustic soda with aq. menthae piperitae, 50 timing. every 2 hours, with the entire treatment lasting from 2 to 3 months. Caustic alkali has a threefold effect on the stomach wall: 1) neutralizing, 2) destroying enzymes and 3) cauterizing. It is tolerated by patients very well and usually gives a quick effect in the sense of the disappearance of pain and other subjective disorders.

Cocaine and Amphetamine Regulated Transcript (CART) Expression Changes in the Stomach Wall Affected by Experimentally Induced Gastric Ulcerations

Cocaine- and amphetamine-regulated transcript (CART) is a peptide suggested to play a role in gastrointestinal tract tissue reaction to pathology. Gastric ulceration is a common disorder affecting huge number of people, and additionally, it contributes to the loss of pig livestock production. Importantly, ulceration as a focal disruption affecting deeper layers of the stomach wall differs from other gastrointestinal pathologies and should be studied individually. The pig’s gastrointestinal tract, due to its many similarities to the human counterpart, provides a valuable experimental model for studying digestive system pathologies. To date, the role of CART in gastric ulceration and the expression of the gene encoding CART in porcine gastrointestinal tube are completely unknown. Therefore, we aimed to verify the changes in the CART expression by Q-PCR (gene encoding CART in the tissue) and double immunofluorescence staining combined with confocal microscopy (CART immunofluorescence in enteric nervous system) in the porcine stomach tissues adjacent to gastric ulcerations. Surprisingly, we found that gastric ulcer caused a significant decrease in the expression of CART-encoding gene and huge reduction in the percentage of CART-immunofluorescent myenteric perikarya and neuronal fibers located within the circular muscle layer. Our results indicate a unique CART-dependent gastric response to ulcer disease.

Two cases of gastric penetration by fish bones with differing courses

ABSTRACT Most foreign bodies swallowed accidentally are naturally excreted. Gastric penetration by a fish bone is rare due to anatomical and physiological features such as the thick stomach wall, wide lumen and gastric acid. The most common penetration site is the antral region. The clinical course of fish bone penetration of the gastric antrum may differ depending on the direction of penetration. In this report, two cases of gastric perforation by fish bones that followed different courses are presented. One case was treated conservatively with antibiotics alone, and another case was considered for surgery, due to increased hematoma and penetration of the fish bone into the pancreas. However, the patient’s comorbidities were so severe that surgery was not possible, resulting in meticulous follow-up. Diagnostic imaging was important in these cases.

LARVAE AND ADULTS OF ASCAROPS SP. (NEMATODA: SPIROCERCIDAE) COLLECTED FROM THE STOMACH OF MAXOMYS WHITEHEADI (RODENTIA: MURIDAE) IN KALIMANTAN, INDONESIA

Third-stage larvae and adults of spiruroid nematodes were found from the stomach wall and stomach lumen, respectively, of Maxomys whiteheadi (Rodentia: Murinae) captured in Bukit Soeharto, Kalimantan, Indonesia. Close observation using light microscope and scanning electron microscope (SEM) revealed that it belongs to the genus Ascarops (Nematoda: Spirocercidae), possibly to Ascarops strongylina (Rudolphi, 1819). It is presumed that this species is parasitic in wild boars, Sus barbatus, in the forest of Kalimantan, and utilizes the murine as a paratenic host, in which it usually remains as third larval stage but can occasionally develop to adult stage.

Difficulties in diagnosing a rupture of the left dome of the diaphragm

Rupture of the diaphragm dome in severe combined trauma is an infrequent phenomenon. At the same time, untimely diagnosis in the acute period leads to various serious complications. The article presents a clinical case of treatment of a patient after a severe railway injury, when a rupture of the left dome of the diaphragm with gastric dislocation was not recognized for 4 months. Pain syndrome due to the patient’s empyema of the pleura and necrosis of the stomach wall was stopped for a long time by taking strong drugs.