scholarly journals Comparison of cytotoxicity among pectenotoxin-2 and other oxidized pectenotoxins in a rat myoblast cell line (L6) and a human rhabdomyosarcoma cell line (RD)

2015 ◽  
Vol 2 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Ryoji Matsushima ◽  
Saori Kikutsugi ◽  
Ryuichi Watanabe ◽  
Hajime Uchida ◽  
Takeshi Yasumoto ◽  
...  
Keyword(s):  
Cell Line ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Myoblast Cell Line ◽  
Myoblast Cell ◽  
Pectenotoxin 2

scholarly journals Tumor necrosis factor (TNF)-induced protein phosphorylation in a human rhabdomyosarcoma cell line is mediated by 60-kD TNF receptors (TR60)

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2211-2220 ◽  
Author(s):  
A Mire-Sluis ◽  
A Meager
Keyword(s):  
Tumor Necrosis Factor ◽  
Protein Phosphorylation ◽  
Cell Line ◽  
Tumor Necrosis ◽  
Tnf Alpha ◽  
Tnf Receptors ◽  
Protein Kinase C Inhibitors ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Necrosis Factor

Abstract In the present study, we used a cloned derivative, KYM-1D4, of the human rhabdomyosarcoma cell line, KYM-1, known to express high numbers of the two tumor necrosis factor (TNF) receptors, TR60 and TR80, and to be highly sensitive to TNF alpha-mediated cytotoxicity/antiproliferation, to investigate the role of TR60 and TR80 in protein phosphorylation. Using permeabilized KYM-1D4 cells, it was found that TNF alpha strongly induced phosphorylation of proteins of molecular weight 80, 65, 58, 42, and 30 kD. Addition of a monoclonal antibody (MoAb) against TR60 was shown to induce cytotoxicity/antiproliferation in KYM-1D4 cells and the same pattern of protein phosphorylation as TNF alpha, whereas addition of an MoAb against TR80 was both noncytotoxic and ineffective in inducing protein phosphorylation. In contrast, in a highly TNF alpha-resistant KYM-1- derived cell line, 37B8R, no protein phosphorylation was induced with either TNF alpha or the agonistic anti-TR60 MoAb. However, when 37B8R was allowed to revert to partial TNF sensitivity by culture in the absence of TNF alpha, the resultant cell line, 37B8S, was found to regain inducibility of protein phosphorylation by TNF alpha. These results indicate that expression of functional TR60 in KYM-1-related cell lines is principally involved in TNF-mediated cytotoxicity/antiproliferation and is necessary for the induction of protein phosphorylation. Nevertheless, the latter, although apparently strongly associated with cytotoxicity, was probably involved in protective mechanisms because protein kinase C inhibitors that inhibited TNF alpha and anti-TR60-induced phosphorylation increased the cytotoxic/antiproliferative response to these mediators.

scholarly journals Biosynthesis of type V procollagen by A204, a human rhabdomyosarcoma cell line.

1982 ◽  
Vol 257 (15) ◽  
pp. 9016-9024 ◽  
Author(s):  
K Alitalo ◽  
R Myllyla ◽  
H Sage ◽  
P Pritzl ◽  
A Vaheri ◽  
...  
Keyword(s):  
Cell Line ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Type V

scholarly journals Fetal calf serum and retinoic acid affect proliferation and terminal differentiation of a rat rhabdomyosarcoma cell line (BA-HAN-1C)

1989 ◽  
Vol 59 (1) ◽  
pp. 61-67 ◽  
Author(s):  
CD Gerharz ◽  
HE Gabbert ◽  
HK Biesalski ◽  
R Engers ◽  
C Luley
Keyword(s):  
Retinoic Acid ◽  
Cell Line ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Terminal Differentiation ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell

scholarly journals Lentivector-Mediated Transfer of Bmi-1 and Telomerase in Muscle Satellite Cells Yields a Duchenne Myoblast Cell Line with Long-Term Genotypic and Phenotypic Stability

2003 ◽  
Vol 14 (16) ◽  
pp. 1525-1533 ◽  
Author(s):  
Christophe Cudré-Mauroux ◽  
Teresa Occhiodoro ◽  
Stéphane König ◽  
Patrick Salmon ◽  
Laurent Bernheim ◽  
...  
Keyword(s):  
Cell Line ◽  
Satellite Cells ◽  
Phenotypic Stability ◽  
Muscle Satellite Cells ◽  
Myoblast Cell Line ◽  
Myoblast Cell

scholarly journals Geigerin-induced cytotoxicity in a murine myoblast cell line (C2C12)

2017 ◽  
Vol 84 (1) ◽  
Author(s):  
Christo J. Botha ◽  
Sarah J. Clift ◽  
Gezina C.H. Ferreira ◽  
Mxolisi G. Masango
Keyword(s):  
Flow Cytometry ◽  
Cell Line ◽  
Ethical Issues ◽  
Sesquiterpene Lactones ◽  
Annexin V ◽  
Metabolic Activation ◽  
Suitable Model ◽  
Myoblast Cell Line ◽  
Myoblast Cell

Geigeria poisoning in sheep, locally known as ‘vermeersiekte’, is an economically important plant poisoning in southern Africa. The toxic principles contained by the toxic plants are believed to be several sesquiterpene lactones, such as geigerin, vermeeric acid and vermeerin, which cause striated muscle lesions in small stock. Because of ethical issues surrounding the use of live animals in toxicity studies, there is currently a dire need to establish an in vitro model that can be used to replace traditional animal experimentation. The objective of this study was to determine the cytotoxicity of geigerin in a murine myoblast cell line (C2C12) using methyl-thiazol-tetrazolium (MTT) and lactate dehydrogenase (LDH) assays, annexin V and propidium iodide (PI) flow cytometry and transmission electron microscopy (TEM). Mouse myoblasts were exposed to 2.0 mM, 2.5 mM and 5.0 mM geigerin for 24, 48 and 72 h. A concentration-dependent cytotoxic response was observed. Apoptosis was detected by means of annexin V flow cytometry during the first 24 h and apoptotic bodies were also visible on TEM. According to the LDH and PI flow cytometry results, myoblast cell membranes were not injured. We concluded that the murine myoblast cell line (C2C12) is a suitable model for future studies planned to evaluate the cytotoxicity of other and combinations of sesquiterpene lactones, with and without metabolic activation, implicated in ‘vermeersiekte’ and to elucidate the subcellular effects of these myotoxins on cultured myoblasts.

Biosynthesis of 10 kDa and 7.5 kDa insulin-like growth factor II in a human rhabdomyosarcoma cell line

1993 ◽  
Vol 93 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Finn C. Nielsen ◽  
Gisela Haselbacher ◽  
Jan Christiansen ◽  
Mats Lake ◽  
Mette Grønborg ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Insulin Like Growth Factor ◽  
Rhabdomyosarcoma Cell Line ◽  
Factor Ii ◽  
Rhabdomyosarcoma Cell

Glucose Regulated Production of Human Insulin in Genetically Modified Myoblast Cell Line (C2C12)

2006 ◽  
Vol 21 (6) ◽  
pp. 526
Author(s):  
Seungjoon Oh ◽  
Jeong-Taek Woo ◽  
Sung Woon Kim ◽  
Jin-Woo Kim ◽  
Young Seol Kim
Keyword(s):  
Cell Line ◽  
Human Insulin ◽  
Genetically Modified ◽  
Myoblast Cell Line ◽  
Myoblast Cell

scholarly journals Transport Mechanism for L-Lactic Acid in Human Myocytes Using Human Prototypic Embryonal Rhabdomyosarcoma Cell Line (RD Cells)

2005 ◽  
Vol 28 (7) ◽  
pp. 1197-1201 ◽  
Author(s):  
Masaki Kobayashi ◽  
Itaru Fujita ◽  
Shirou Itagaki ◽  
Takeshi Hirano ◽  
Ken Iseki
Keyword(s):  
Lactic Acid ◽  
Cell Line ◽  
Transport Mechanism ◽  
Embryonal Rhabdomyosarcoma ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Rd Cells

Cartap-induced cytotoxicity in mouse C2C12 myoblast cell line and the roles of calcium ion and oxidative stress on the toxic effects

Toxicology ◽  
2006 ◽  
Vol 219 (1-3) ◽  
pp. 73-84 ◽  
Author(s):  
Jiunn-Wang Liao ◽  
Jaw-Jou Kang ◽  
Chian-Ren Jeng ◽  
Shao-Kuang Chang ◽  
Ming-Jang Kuo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Line ◽  
Calcium Ion ◽  
Toxic Effects ◽  
C2c12 Myoblast ◽  
Myoblast Cell Line ◽  
Myoblast Cell ◽  
And Oxidative Stress
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